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The map label for this gene is hsaD [H]

Identifier: 66046330

GI number: 66046330

Start: 3718736

End: 3719734

Strand: Reverse

Name: hsaD [H]

Synonym: Psyr_3101

Alternate gene names: 66046330

Gene position: 3719734-3718736 (Counterclockwise)

Preceding gene: 66046332

Following gene: 66046327

Centisome position: 61.04

GC content: 59.46

Gene sequence:

>999_bases
ATGCGGGCTGCAAAGATTGCCGGCATGTTGATGTCGTTGACGCTGTCAGGCGCAGCACTGGCTGAGACGCCCGGTTATGG
CCAGCAACTGGAAGGTTTCACGTACCCCCATGCGCTGCACAAATTCAGTTTCCAGTCCCAGGGCCAGACGCTGGAAATGG
GTTATATGGACGTCGCGCCCAGTGGCAAAGCCAACGGCCGCACGGCGGTGCTGATGCATGGCAAGAATTTTTGCGCTGCG
ACCTGGGAAGACACCATCAAGGGACTGAGTGCCGCGGGCTATCGCGTCGTCGCAGCGGATCAGATCGGTTTCTGCACCTC
GACCAAGCCGGAGCACTACCAGTACAGCTTCCAGCAGTTGTCGATGAACACCCACGCGCTGCTGGAAAAACTGGGCCTCG
ACAAGGTGTCGGTGGTCGGTCACTCCACGGGCGGCATGCTGGCCACGCGTTACGCATTGTTGTACCCGAAGCAGACCGAG
AAGCTGGTGATGGTCAACCCTATCGGTCTGGAAGACTGGAAAGCACTCGGCGTGCCGTACCGCAGCGTCGACCAGTGGTA
TGAGCGAGAGCTGAAGACCACCGCCGAAGGCATTCGCGCCTACGAGCAGAAGACTTACTACGACGGCCGCTGGAAGCCTG
AGTATGACAAGTGGGTCGACATGCTGGCCGGTTTGAACAAAGGGCCGGGTCACAAGGTCGTGGCCTGGAACTCGGCGTTG
ATCTACGACATGATTTTCACCCAGCCGGTGTTCTACGAGTTCCCCAGGTTGCAGGTGCCGACGGTGCTGATGATTGGCGA
TGCCGATACCACTGCCATCGGCAGCGACATCGCCCCGCCTGAGGTCAAGGCGAAGATCGGCAATTACAAGGTACTGGGCA
AGCAGGTGGCGCAGATGATCCCGGGCGCCAGGCTGGTGGAATTCAAGGGCAAGGGCCATGCCCCGCAGATGGAAGACCCG
CAGGGCTTCAACAAGGCACTGGTCTCCGAACTTCAATAA

Upstream 100 bases:

>100_bases
AAAGGGCGAATCTGTTGTGGGAAACAGGGTCTACTTGCAGGTCCGCCGCGTGCAGTTCAGCGGTTCGAGAATTCGACTCT
ATTTACAGGAGGTGCCCCTT

Downstream 100 bases:

>100_bases
GCTGCACGTCATGCCACGCGGCAACCTGGTCCGATAGCGACCAGGTTGTCTGCGACCGGCATTACCGATTAAACGGCAAT
CTCTACCTGATCACCTTTGA

Product: Alpha/beta hydrolase fold

Products: NA

Alternate protein names: 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase; HOPDA hydrolase; Meta-cleavage product hydrolase; MCP hydrolase [H]

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MRAAKIAGMLMSLTLSGAALAETPGYGQQLEGFTYPHALHKFSFQSQGQTLEMGYMDVAPSGKANGRTAVLMHGKNFCAA
TWEDTIKGLSAAGYRVVAADQIGFCTSTKPEHYQYSFQQLSMNTHALLEKLGLDKVSVVGHSTGGMLATRYALLYPKQTE
KLVMVNPIGLEDWKALGVPYRSVDQWYERELKTTAEGIRAYEQKTYYDGRWKPEYDKWVDMLAGLNKGPGHKVVAWNSAL
IYDMIFTQPVFYEFPRLQVPTVLMIGDADTTAIGSDIAPPEVKAKIGNYKVLGKQVAQMIPGARLVEFKGKGHAPQMEDP
QGFNKALVSELQ

Sequences:

>Translated_332_residues
MRAAKIAGMLMSLTLSGAALAETPGYGQQLEGFTYPHALHKFSFQSQGQTLEMGYMDVAPSGKANGRTAVLMHGKNFCAA
TWEDTIKGLSAAGYRVVAADQIGFCTSTKPEHYQYSFQQLSMNTHALLEKLGLDKVSVVGHSTGGMLATRYALLYPKQTE
KLVMVNPIGLEDWKALGVPYRSVDQWYERELKTTAEGIRAYEQKTYYDGRWKPEYDKWVDMLAGLNKGPGHKVVAWNSAL
IYDMIFTQPVFYEFPRLQVPTVLMIGDADTTAIGSDIAPPEVKAKIGNYKVLGKQVAQMIPGARLVEFKGKGHAPQMEDP
QGFNKALVSELQ
>Mature_332_residues
MRAAKIAGMLMSLTLSGAALAETPGYGQQLEGFTYPHALHKFSFQSQGQTLEMGYMDVAPSGKANGRTAVLMHGKNFCAA
TWEDTIKGLSAAGYRVVAADQIGFCTSTKPEHYQYSFQQLSMNTHALLEKLGLDKVSVVGHSTGGMLATRYALLYPKQTE
KLVMVNPIGLEDWKALGVPYRSVDQWYERELKTTAEGIRAYEQKTYYDGRWKPEYDKWVDMLAGLNKGPGHKVVAWNSAL
IYDMIFTQPVFYEFPRLQVPTVLMIGDADTTAIGSDIAPPEVKAKIGNYKVLGKQVAQMIPGARLVEFKGKGHAPQMEDP
QGFNKALVSELQ

Specific function: Catalyzes the hydrolysis of a carbon-carbon bond in 4,5- 9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oic acid (4,9-DSHA) to yield 9,17-dioxo-1,2,3,4,10,19- hexanorandrostan-5-oic acid (DOHNAA) and 2-hydroxy-hexa-2,4- dienoic acid (HHD). Is

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. HsaD family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.7.1.8 [H]

Molecular weight: Translated: 36674; Mature: 36674

Theoretical pI: Translated: 8.53; Mature: 8.53

Prosite motif: PS00120 LIPASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAAKIAGMLMSLTLSGAALAETPGYGQQLEGFTYPHALHKFSFQSQGQTLEMGYMDVAP
CCHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCHHHHHHCCCCCCCCEEEECEEEECC
SGKANGRTAVLMHGKNFCAATWEDTIKGLSAAGYRVVAADQIGFCTSTKPEHYQYSFQQL
CCCCCCCEEEEEECCCCEEECHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHH
SMNTHALLEKLGLDKVSVVGHSTGGMLATRYALLYPKQTEKLVMVNPIGLEDWKALGVPY
CCCHHHHHHHHCCCEEEEEECCCCCHHHHHEEEECCCCCCCEEEECCCCCCCHHHHCCCH
RSVDQWYERELKTTAEGIRAYEQKTYYDGRWKPEYDKWVDMLAGLNKGPGHKVVAWNSAL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCHH
IYDMIFTQPVFYEFPRLQVPTVLMIGDADTTAIGSDIAPPEVKAKIGNYKVLGKQVAQMI
HHHHHHCCHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHC
PGARLVEFKGKGHAPQMEDPQGFNKALVSELQ
CCCEEEEECCCCCCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MRAAKIAGMLMSLTLSGAALAETPGYGQQLEGFTYPHALHKFSFQSQGQTLEMGYMDVAP
CCHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCHHHHHHCCCCCCCCEEEECEEEECC
SGKANGRTAVLMHGKNFCAATWEDTIKGLSAAGYRVVAADQIGFCTSTKPEHYQYSFQQL
CCCCCCCEEEEEECCCCEEECHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHH
SMNTHALLEKLGLDKVSVVGHSTGGMLATRYALLYPKQTEKLVMVNPIGLEDWKALGVPY
CCCHHHHHHHHCCCEEEEEECCCCCHHHHHEEEECCCCCCCEEEECCCCCCCHHHHCCCH
RSVDQWYERELKTTAEGIRAYEQKTYYDGRWKPEYDKWVDMLAGLNKGPGHKVVAWNSAL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCHH
IYDMIFTQPVFYEFPRLQVPTVLMIGDADTTAIGSDIAPPEVKAKIGNYKVLGKQVAQMI
HHHHHHCCHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHC
PGARLVEFKGKGHAPQMEDPQGFNKALVSELQ
CCCEEEEECCCCCCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]