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The map label for this gene is pilR [H]

Identifier: 66046242

GI number: 66046242

Start: 3604772

End: 3606097

Strand: Reverse

Name: pilR [H]

Synonym: Psyr_3011

Alternate gene names: 66046242

Gene position: 3606097-3604772 (Counterclockwise)

Preceding gene: 66046244

Following gene: 66046239

Centisome position: 59.18

GC content: 61.24

Gene sequence:

>1326_bases
ATGCATGGATCGGCCCCAACACGACGTCTGTTAATGCTCGACGGCGGTGACGACTGTCATCCATTGATCGCAGTTCTGAT
CGCAGCCGGCTGGACGGTGCAGGAAAGCCCATCGGGTTCGGCAATCATTTCTGATTGTGATGTAGGCCTCATTCACCTGA
CGTCCGATCATTTTGAACACGTGTCCAGGCTCGAGGCGCTGTTCGTTAACAGCCCCGTGCGCTGGATTGCAGTGCTGTCA
GCCAGTGATTTGCGACGTGAGAAAATCGGCGATTTCGTCTGCCGCTGGTTTTTCGACTTCCATACGCTGCCTTTCGATGC
AGGTCGGCTGCATGCCTCGCTGGACAGAGCATTGACTGCCGGGCACCCGAATGTGCAGGGCGCGCCATCGCAATTCAGCG
CGGAGCCCGAGCTGCTCGGCGACAGTCGGCCTGTCCGTGAATTGCGCAGGCTGCTGAGCAAACTGGCGCCCACCGATTCG
CCGGTACTGATCAGGGGCGAGCGGGGCACGGGCAAGGAACTGATCGCGCGCAGCCTGCATACTCAGTCCTTGCGACGTGA
CAAACCCTTTATCGTCGTTGATTGCGCAACGCCTGACGGGCAGTCGATCCACGCCGTGCTGTTCGGTCATGAGGACGGCG
AATTTGACGATGCTCAAGAGCGCAGGGTCGGGCTGCTTGAGGCCGCAGACGGCGGCACGCTGTTGCTCGATGAGGTCGGT
GAGCTCCCGCTGGATACCCAGGCGAGCCTGCTGCGCTTTCTCGAAGACCGGCAGATCGAACGCGCGGACGGCGGCGAACC
GATTGCAGTGGATGTGCGGGTGCTGGCCGCCACTCGCGAGGATCTTGAAACGGCCGTACGCAAAAAGCGCTTTCGCGATG
ACCTGTATTACCAGCTCAATGTACTGCAGGTCGGTGTAGCGCCGCTGCGCGAGCGCCATGGCGATCTGGCGCTGCTGGCT
AACCATTTCGCCCATTTGTACAGTCAGGAAACCGGCAGGCGGGCGCGCAGTTTCAGTCAGGATGCGCTGGTGGCGCTGGG
CAAGCACGATTGGCCGGGCAATGTGCGCGAACTGGCCGGTCGCGTTCGGCGCGGGCTGGTGCTGGCCGAAGGCAGGCAGA
TAGAGGCGGCAAACCTGGGCCTGCTTGGCGAAGAGGACAGCATTGGCAGCATGGGGACGCTGGAGGATTACAAGTCGCGT
GCCGAGCGTCAGGCGTTGTGCGACGTGTTGACGCGCCACAGTGACAATTTGAGTGTGGCGGCCAGAGTGCTGGGTATTTC
GCGGCCAACGTTCTATCGGCTGTTACACAAGCACCAGATCCGCTGA

Upstream 100 bases:

>100_bases
CGCGTCAAATATTTGCTAGGGTGATGGTTGGGCTCGCCAACAGACTGTTTATAAAAAGCTTTTAAAAATCCCTCCATACG
CCGGGCGCCAAGGAAACTTC

Downstream 100 bases:

>100_bases
GTCGCCCAGCAACTGTGAGACAGCGGTCAGCGCCTGTTCGCGGCGCTGCTCCCAGTTACCGCCCAGTACCCGGTAAGCCT
GCAGGTGGTGTTCCAGCCAT

Product: helix-turn-helix, Fis-type

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MHGSAPTRRLLMLDGGDDCHPLIAVLIAAGWTVQESPSGSAIISDCDVGLIHLTSDHFEHVSRLEALFVNSPVRWIAVLS
ASDLRREKIGDFVCRWFFDFHTLPFDAGRLHASLDRALTAGHPNVQGAPSQFSAEPELLGDSRPVRELRRLLSKLAPTDS
PVLIRGERGTGKELIARSLHTQSLRRDKPFIVVDCATPDGQSIHAVLFGHEDGEFDDAQERRVGLLEAADGGTLLLDEVG
ELPLDTQASLLRFLEDRQIERADGGEPIAVDVRVLAATREDLETAVRKKRFRDDLYYQLNVLQVGVAPLRERHGDLALLA
NHFAHLYSQETGRRARSFSQDALVALGKHDWPGNVRELAGRVRRGLVLAEGRQIEAANLGLLGEEDSIGSMGTLEDYKSR
AERQALCDVLTRHSDNLSVAARVLGISRPTFYRLLHKHQIR

Sequences:

>Translated_441_residues
MHGSAPTRRLLMLDGGDDCHPLIAVLIAAGWTVQESPSGSAIISDCDVGLIHLTSDHFEHVSRLEALFVNSPVRWIAVLS
ASDLRREKIGDFVCRWFFDFHTLPFDAGRLHASLDRALTAGHPNVQGAPSQFSAEPELLGDSRPVRELRRLLSKLAPTDS
PVLIRGERGTGKELIARSLHTQSLRRDKPFIVVDCATPDGQSIHAVLFGHEDGEFDDAQERRVGLLEAADGGTLLLDEVG
ELPLDTQASLLRFLEDRQIERADGGEPIAVDVRVLAATREDLETAVRKKRFRDDLYYQLNVLQVGVAPLRERHGDLALLA
NHFAHLYSQETGRRARSFSQDALVALGKHDWPGNVRELAGRVRRGLVLAEGRQIEAANLGLLGEEDSIGSMGTLEDYKSR
AERQALCDVLTRHSDNLSVAARVLGISRPTFYRLLHKHQIR
>Mature_441_residues
MHGSAPTRRLLMLDGGDDCHPLIAVLIAAGWTVQESPSGSAIISDCDVGLIHLTSDHFEHVSRLEALFVNSPVRWIAVLS
ASDLRREKIGDFVCRWFFDFHTLPFDAGRLHASLDRALTAGHPNVQGAPSQFSAEPELLGDSRPVRELRRLLSKLAPTDS
PVLIRGERGTGKELIARSLHTQSLRRDKPFIVVDCATPDGQSIHAVLFGHEDGEFDDAQERRVGLLEAADGGTLLLDEVG
ELPLDTQASLLRFLEDRQIERADGGEPIAVDVRVLAATREDLETAVRKKRFRDDLYYQLNVLQVGVAPLRERHGDLALLA
NHFAHLYSQETGRRARSFSQDALVALGKHDWPGNVRELAGRVRRGLVLAEGRQIEAANLGLLGEEDSIGSMGTLEDYKSR
AERQALCDVLTRHSDNLSVAARVLGISRPTFYRLLHKHQIR

Specific function: Member of the two-component regulatory system pilS/pilR that regulates the expression of type 4 fimbriae. When activated by pilS it acts in conjunction with sigma-54 as a transcriptional activator [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790437, Length=434, Percent_Identity=33.4101382488479, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI1788550, Length=358, Percent_Identity=38.268156424581, Blast_Score=223, Evalue=3e-59,
Organism=Escherichia coli, GI1789233, Length=333, Percent_Identity=37.8378378378378, Blast_Score=218, Evalue=5e-58,
Organism=Escherichia coli, GI1790299, Length=367, Percent_Identity=36.2397820163488, Blast_Score=207, Evalue=2e-54,
Organism=Escherichia coli, GI1788905, Length=348, Percent_Identity=35.9195402298851, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI87082117, Length=311, Percent_Identity=39.2282958199357, Blast_Score=201, Evalue=6e-53,
Organism=Escherichia coli, GI1789087, Length=318, Percent_Identity=36.7924528301887, Blast_Score=191, Evalue=9e-50,
Organism=Escherichia coli, GI87081872, Length=318, Percent_Identity=37.4213836477987, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1786524, Length=316, Percent_Identity=35.7594936708861, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI1787583, Length=307, Percent_Identity=34.5276872964169, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI87082152, Length=243, Percent_Identity=40.7407407407407, Blast_Score=164, Evalue=8e-42,
Organism=Escherichia coli, GI87081858, Length=303, Percent_Identity=29.042904290429, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1789828, Length=223, Percent_Identity=32.7354260089686, Blast_Score=117, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 48819; Mature: 48819

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00676 SIGMA54_INTERACT_2 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHGSAPTRRLLMLDGGDDCHPLIAVLIAAGWTVQESPSGSAIISDCDVGLIHLTSDHFEH
CCCCCCCCEEEEEECCCCHHHHHHHHHHCCCEEECCCCCCEEEECCCEEEEEECCHHHHH
VSRLEALFVNSPVRWIAVLSASDLRREKIGDFVCRWFFDFHTLPFDAGRLHASLDRALTA
HHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHC
GHPNVQGAPSQFSAEPELLGDSRPVRELRRLLSKLAPTDSPVLIRGERGTGKELIARSLH
CCCCCCCCCCCCCCCCHHCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHH
TQSLRRDKPFIVVDCATPDGQSIHAVLFGHEDGEFDDAQERRVGLLEAADGGTLLLDEVG
HHHHHCCCCEEEEECCCCCCCCEEEEEEECCCCCCCCHHHHHCCEEEECCCCEEEHHHHC
ELPLDTQASLLRFLEDRQIERADGGEPIAVDVRVLAATREDLETAVRKKRFRDDLYYQLN
CCCCCCHHHHHHHHHHCCHHHCCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHEEEE
VLQVGVAPLRERHGDLALLANHFAHLYSQETGRRARSFSQDALVALGKHDWPGNVRELAG
HHHHCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEECCCCCCCCHHHHHH
RVRRGLVLAEGRQIEAANLGLLGEEDSIGSMGTLEDYKSRAERQALCDVLTRHSDNLSVA
HHHHCEEEECCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHH
ARVLGISRPTFYRLLHKHQIR
HHHHHCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MHGSAPTRRLLMLDGGDDCHPLIAVLIAAGWTVQESPSGSAIISDCDVGLIHLTSDHFEH
CCCCCCCCEEEEEECCCCHHHHHHHHHHCCCEEECCCCCCEEEECCCEEEEEECCHHHHH
VSRLEALFVNSPVRWIAVLSASDLRREKIGDFVCRWFFDFHTLPFDAGRLHASLDRALTA
HHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHC
GHPNVQGAPSQFSAEPELLGDSRPVRELRRLLSKLAPTDSPVLIRGERGTGKELIARSLH
CCCCCCCCCCCCCCCCHHCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHH
TQSLRRDKPFIVVDCATPDGQSIHAVLFGHEDGEFDDAQERRVGLLEAADGGTLLLDEVG
HHHHHCCCCEEEEECCCCCCCCEEEEEEECCCCCCCCHHHHHCCEEEECCCCEEEHHHHC
ELPLDTQASLLRFLEDRQIERADGGEPIAVDVRVLAATREDLETAVRKKRFRDDLYYQLN
CCCCCCHHHHHHHHHHCCHHHCCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHEEEE
VLQVGVAPLRERHGDLALLANHFAHLYSQETGRRARSFSQDALVALGKHDWPGNVRELAG
HHHHCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEECCCCCCCCHHHHHH
RVRRGLVLAEGRQIEAANLGLLGEEDSIGSMGTLEDYKSRAERQALCDVLTRHSDNLSVA
HHHHCEEEECCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHH
ARVLGISRPTFYRLLHKHQIR
HHHHHCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1317379; 8097014; 7911557; 10984043 [H]