Click here to switch to the map view.

The map label for this gene is glgX [H]

Identifier: 66046228

GI number: 66046228

Start: 3592521

End: 3594704

Strand: Direct

Name: glgX [H]

Synonym: Psyr_2997

Alternate gene names: 66046228

Gene position: 3592521-3594704 (Clockwise)

Preceding gene: 66046227

Following gene: 66046229

Centisome position: 58.95

GC content: 59.62

Gene sequence:

>2184_bases
ATGAATACGAAGTCCACTACACCGGCAGAAGATAATTCAGCCCCGCTAGACAACCCTGGCAGTACCCCGTCTCGCATTCG
TGAAGGACTTCCCTTTCCTCTCGGTGCAAGCTGGGATGGCCTGGGCGTCAACTTCGCCCTGTTCTCGGCAAACGCCACCA
AGGTAGAGCTGTGCCTGTTCGACTCCACTGGCGAAATCGAACTGGAACGCATCGAACTTCCGGAATACACCGACGAGATT
TACCACGGCTATCTGCCGGATGCGCACCCGGGCCAGATCTACGGCTATCGCGTGTACGGCCCGTACGATCCGAAGAATGG
TCATCGTTTCAACCACAACAAGCTGCTGATCGACCCGTACGCCAAGCAACTGGTCGGCGAACTGAAGTGGTCGGAAGCGC
TGTTCGGTTACACCATCGGCCATCCTGACGGCGACCTGAGCTTCGACGAGCGCGACAGCGCCCCTTTCGTACCGAAAAGC
AAGGTGATCGATGAGGCCTACACCTGGGGCCGTGATCAGCGCGTTGGCACGCCTTGGGATAAAACGATTTTCTACGAAAC
CCACGTACGCGGCATCACCATGCGTCACCCTGAGGTGGCCGAAGAGCTGCGCGGCACGTTCGCCGGTCTCGGATCAGCGC
CGGTCGTCGAGCACATTCGCAAGCTGGGTGTGACCTCCGTCGAGCTGCTGCCGATTCATGCCTTCGTCAACGACCAGCAT
TTGCTGCAAAAGGGCATGACCAACTACTGGGGCTACAACAGCATCGCGTTCTTCGCCCCGGACCCGCGTTACCTGGCAAG
CGGCAAGATTGCCGAGTTCAAGGAAATGGTTGCGCACCTGCACCACGCCGGTCTCGAGGTCATCCTCGATGTGGTCTACA
ACCACACCGCCGAAGGCAACGAGCTGGGCCCTACCCTGTCGATGCGTGGTATCGACAACGCCTCGTACTACCGCCTGATG
CCGGACGACAAGCGCTACTACATCAACGACTCGGGCACTGGCAACACCCTCGACCTGAGCCACCCTTGCGTATTGCAAAT
GGTCACCGACTCGCTGCGCTACTGGGCTTCGGAAATGCACGTCGACGGCTTCCGCTTCGACCTGGCGACCATTCTGGGCC
GCTACCACGATGGTTTCGACGAGCGCCACAGCTTCCTGGTCGCCTGCCGTCAGGATCCGGTGCTGCGTCAGGTCAAACTG
GTCGCCGAGCCGTGGGACTGCGGTCCGGGCGGCTACCAGGTGGGCGGTTTCCCACCAGGCTGGATGGAATGGAACGACAA
GTTCCGCGACACGGTTCGTGCCTTCTGGAAAGGCGACGAAGCTCAACTGGCCGACTTCGCTGCGCGCATGACTGCCTCGG
GCAACATGTTCAACCAGCGCGGTCGTCGTCCGCAGGCATCCGTGAACTTCATCACTGCTCACGATGGTTTCACGCTGCAT
GACCTGGTGTCGTACAACGACAAGCACAACGAAGCCAACGACGAAAACAACCAGGATGGCAGCAACAACAACCTGTCCTG
GAACCATGGCGTCGAAGGTCCGACCGAAGATCCGGAAATCAACAGCCTGCGTCTGCGCCAGATGCGTAACTTCTTCGCCA
CCCTGCTGCTGGCACAAGGCACGCCGATGATTGTTGCGGGCGACGAGTTCGCGCGCACTCAGCACGGCAACAACAATGCC
TACTGCCAGGACAGCGAGATTGGCTGGATCAACTGGGATCTGGACGAAGATGGCGAAGCGCTGCTCAAGTTCGTCACCCG
CGTGATCAAGCTGCGTCAGACCTACCCGATCCTGCGTCGCAGCCGCTTCCTGGTAGGCGACTACAACGAAGAGATCGGCG
TCAAGGACGTCACCTGGCTTGCTCCGGATGGCAACGAGATGAGTGTCGAGCAGTGGCACGATGCCAATGGCCGTTGCCTG
GGCATGCTGATGGATGGTCGCGCACAGGAAACCGGTATCCGTCGCGCGGGTGCCGACGCCACGCTGTTGCTGGTGGTCAA
CGCGCATCACGACGGCGTCAACTTCACCCTGCCGGAAGTGCCTGAAGGTACCCATTACGAGTGCCTGATCGACACCAATC
AGGATGATGCACGCAGCAAGGAGCACTTTGCGTTCGGTTCGCAATACACCGTAACGCCGCGTTCCCTGCTGCTGTTCGAA
CTGAAGCGTGAAGACCAGCAATGA

Upstream 100 bases:

>100_bases
AGGCCTGCTACACCCCCTTAGATTATCGTCAGTATCGGAGCCCGCGGCTTCGATAGCGGCCTTGGCCGCTCAGAAAATCA
TTCGACGTCAGGAAGTCACC

Downstream 100 bases:

>100_bases
TTGATGCCTTGAGTGAGTTCCTCGACTGGCGCAAGCACGGTTATGCCGATACGCGTGCGCTGGGCGAGTGCTTACAGGCA
TTGGTCAATGAAGGTCTGGA

Product: glycoside hydrolase, family alpha amylase catalytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 727; Mature: 727

Protein sequence:

>727_residues
MNTKSTTPAEDNSAPLDNPGSTPSRIREGLPFPLGASWDGLGVNFALFSANATKVELCLFDSTGEIELERIELPEYTDEI
YHGYLPDAHPGQIYGYRVYGPYDPKNGHRFNHNKLLIDPYAKQLVGELKWSEALFGYTIGHPDGDLSFDERDSAPFVPKS
KVIDEAYTWGRDQRVGTPWDKTIFYETHVRGITMRHPEVAEELRGTFAGLGSAPVVEHIRKLGVTSVELLPIHAFVNDQH
LLQKGMTNYWGYNSIAFFAPDPRYLASGKIAEFKEMVAHLHHAGLEVILDVVYNHTAEGNELGPTLSMRGIDNASYYRLM
PDDKRYYINDSGTGNTLDLSHPCVLQMVTDSLRYWASEMHVDGFRFDLATILGRYHDGFDERHSFLVACRQDPVLRQVKL
VAEPWDCGPGGYQVGGFPPGWMEWNDKFRDTVRAFWKGDEAQLADFAARMTASGNMFNQRGRRPQASVNFITAHDGFTLH
DLVSYNDKHNEANDENNQDGSNNNLSWNHGVEGPTEDPEINSLRLRQMRNFFATLLLAQGTPMIVAGDEFARTQHGNNNA
YCQDSEIGWINWDLDEDGEALLKFVTRVIKLRQTYPILRRSRFLVGDYNEEIGVKDVTWLAPDGNEMSVEQWHDANGRCL
GMLMDGRAQETGIRRAGADATLLLVVNAHHDGVNFTLPEVPEGTHYECLIDTNQDDARSKEHFAFGSQYTVTPRSLLLFE
LKREDQQ

Sequences:

>Translated_727_residues
MNTKSTTPAEDNSAPLDNPGSTPSRIREGLPFPLGASWDGLGVNFALFSANATKVELCLFDSTGEIELERIELPEYTDEI
YHGYLPDAHPGQIYGYRVYGPYDPKNGHRFNHNKLLIDPYAKQLVGELKWSEALFGYTIGHPDGDLSFDERDSAPFVPKS
KVIDEAYTWGRDQRVGTPWDKTIFYETHVRGITMRHPEVAEELRGTFAGLGSAPVVEHIRKLGVTSVELLPIHAFVNDQH
LLQKGMTNYWGYNSIAFFAPDPRYLASGKIAEFKEMVAHLHHAGLEVILDVVYNHTAEGNELGPTLSMRGIDNASYYRLM
PDDKRYYINDSGTGNTLDLSHPCVLQMVTDSLRYWASEMHVDGFRFDLATILGRYHDGFDERHSFLVACRQDPVLRQVKL
VAEPWDCGPGGYQVGGFPPGWMEWNDKFRDTVRAFWKGDEAQLADFAARMTASGNMFNQRGRRPQASVNFITAHDGFTLH
DLVSYNDKHNEANDENNQDGSNNNLSWNHGVEGPTEDPEINSLRLRQMRNFFATLLLAQGTPMIVAGDEFARTQHGNNNA
YCQDSEIGWINWDLDEDGEALLKFVTRVIKLRQTYPILRRSRFLVGDYNEEIGVKDVTWLAPDGNEMSVEQWHDANGRCL
GMLMDGRAQETGIRRAGADATLLLVVNAHHDGVNFTLPEVPEGTHYECLIDTNQDDARSKEHFAFGSQYTVTPRSLLLFE
LKREDQQ
>Mature_727_residues
MNTKSTTPAEDNSAPLDNPGSTPSRIREGLPFPLGASWDGLGVNFALFSANATKVELCLFDSTGEIELERIELPEYTDEI
YHGYLPDAHPGQIYGYRVYGPYDPKNGHRFNHNKLLIDPYAKQLVGELKWSEALFGYTIGHPDGDLSFDERDSAPFVPKS
KVIDEAYTWGRDQRVGTPWDKTIFYETHVRGITMRHPEVAEELRGTFAGLGSAPVVEHIRKLGVTSVELLPIHAFVNDQH
LLQKGMTNYWGYNSIAFFAPDPRYLASGKIAEFKEMVAHLHHAGLEVILDVVYNHTAEGNELGPTLSMRGIDNASYYRLM
PDDKRYYINDSGTGNTLDLSHPCVLQMVTDSLRYWASEMHVDGFRFDLATILGRYHDGFDERHSFLVACRQDPVLRQVKL
VAEPWDCGPGGYQVGGFPPGWMEWNDKFRDTVRAFWKGDEAQLADFAARMTASGNMFNQRGRRPQASVNFITAHDGFTLH
DLVSYNDKHNEANDENNQDGSNNNLSWNHGVEGPTEDPEINSLRLRQMRNFFATLLLAQGTPMIVAGDEFARTQHGNNNA
YCQDSEIGWINWDLDEDGEALLKFVTRVIKLRQTYPILRRSRFLVGDYNEEIGVKDVTWLAPDGNEMSVEQWHDANGRCL
GMLMDGRAQETGIRRAGADATLLLVVNAHHDGVNFTLPEVPEGTHYECLIDTNQDDARSKEHFAFGSQYTVTPRSLLLFE
LKREDQQ

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=624, Percent_Identity=47.1153846153846, Blast_Score=566, Evalue=1e-162,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 82106; Mature: 82106

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTKSTTPAEDNSAPLDNPGSTPSRIREGLPFPLGASWDGLGVNFALFSANATKVELCLF
CCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEE
DSTGEIELERIELPEYTDEIYHGYLPDAHPGQIYGYRVYGPYDPKNGHRFNHNKLLIDPY
CCCCCEEEEEEECCCHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCEECCCEEEECHH
AKQLVGELKWSEALFGYTIGHPDGDLSFDERDSAPFVPKSKVIDEAYTWGRDQRVGTPWD
HHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCC
KTIFYETHVRGITMRHPEVAEELRGTFAGLGSAPVVEHIRKLGVTSVELLPIHAFVNDQH
CEEEEEECCCEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEEEEEEECCHHH
LLQKGMTNYWGYNSIAFFAPDPRYLASGKIAEFKEMVAHLHHAGLEVILDVVYNHTAEGN
HHHHCCHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
ELGPTLSMRGIDNASYYRLMPDDKRYYINDSGTGNTLDLSHPCVLQMVTDSLRYWASEMH
CCCCEEEECCCCCCCEEEECCCCCEEEECCCCCCCEEECCCCHHHHHHHHHHHHHHHHHC
VDGFRFDLATILGRYHDGFDERHSFLVACRQDPVLRQVKLVAEPWDCGPGGYQVGGFPPG
CCCHHHHHHHHHHHHCCCHHHCCEEEEEECCCHHHHHHHHEECCCCCCCCCEECCCCCCC
WMEWNDKFRDTVRAFWKGDEAQLADFAARMTASGNMFNQRGRRPQASVNFITAHDGFTLH
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHCCCCCCCCEEEEEECCCCCHH
DLVSYNDKHNEANDENNQDGSNNNLSWNHGVEGPTEDPEINSLRLRQMRNFFATLLLAQG
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
TPMIVAGDEFARTQHGNNNAYCQDSEIGWINWDLDEDGEALLKFVTRVIKLRQTYPILRR
CCEEEECCHHHHHCCCCCCCEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
SRFLVGDYNEEIGVKDVTWLAPDGNEMSVEQWHDANGRCLGMLMDGRAQETGIRRAGADA
CEEEEECCCCCCCCCEEEEECCCCCCCCHHHHCCCCCCEEEEEECCCCHHHCHHHCCCCE
TLLLVVNAHHDGVNFTLPEVPEGTHYECLIDTNQDDARSKEHFAFGSQYTVTPRSLLLFE
EEEEEEECCCCCCEEECCCCCCCCCEEEEEECCCCCCCCCHHEECCCEEEECHHHHEEEE
LKREDQQ
EHHCCCC
>Mature Secondary Structure
MNTKSTTPAEDNSAPLDNPGSTPSRIREGLPFPLGASWDGLGVNFALFSANATKVELCLF
CCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEE
DSTGEIELERIELPEYTDEIYHGYLPDAHPGQIYGYRVYGPYDPKNGHRFNHNKLLIDPY
CCCCCEEEEEEECCCHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCEECCCEEEECHH
AKQLVGELKWSEALFGYTIGHPDGDLSFDERDSAPFVPKSKVIDEAYTWGRDQRVGTPWD
HHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCC
KTIFYETHVRGITMRHPEVAEELRGTFAGLGSAPVVEHIRKLGVTSVELLPIHAFVNDQH
CEEEEEECCCEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEEEEEEECCHHH
LLQKGMTNYWGYNSIAFFAPDPRYLASGKIAEFKEMVAHLHHAGLEVILDVVYNHTAEGN
HHHHCCHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
ELGPTLSMRGIDNASYYRLMPDDKRYYINDSGTGNTLDLSHPCVLQMVTDSLRYWASEMH
CCCCEEEECCCCCCCEEEECCCCCEEEECCCCCCCEEECCCCHHHHHHHHHHHHHHHHHC
VDGFRFDLATILGRYHDGFDERHSFLVACRQDPVLRQVKLVAEPWDCGPGGYQVGGFPPG
CCCHHHHHHHHHHHHCCCHHHCCEEEEEECCCHHHHHHHHEECCCCCCCCCEECCCCCCC
WMEWNDKFRDTVRAFWKGDEAQLADFAARMTASGNMFNQRGRRPQASVNFITAHDGFTLH
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHCCCCCCCCEEEEEECCCCCHH
DLVSYNDKHNEANDENNQDGSNNNLSWNHGVEGPTEDPEINSLRLRQMRNFFATLLLAQG
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
TPMIVAGDEFARTQHGNNNAYCQDSEIGWINWDLDEDGEALLKFVTRVIKLRQTYPILRR
CCEEEECCHHHHHCCCCCCCEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
SRFLVGDYNEEIGVKDVTWLAPDGNEMSVEQWHDANGRCLGMLMDGRAQETGIRRAGADA
CEEEEECCCCCCCCCEEEEECCCCCCCCHHHHCCCCCCEEEEEECCCCHHHCHHHCCCCE
TLLLVVNAHHDGVNFTLPEVPEGTHYECLIDTNQDDARSKEHFAFGSQYTVTPRSLLLFE
EEEEEEECCCCCCEEECCCCCCCCCEEEEEECCCCCCCCCHHEECCCEEEECHHHHEEEE
LKREDQQ
EHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]