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The map label for this gene is alkB [H]

Identifier: 66046216

GI number: 66046216

Start: 3576049

End: 3576735

Strand: Direct

Name: alkB [H]

Synonym: Psyr_2985

Alternate gene names: 66046216

Gene position: 3576049-3576735 (Clockwise)

Preceding gene: 66046215

Following gene: 66046221

Centisome position: 58.68

GC content: 62.74

Gene sequence:

>687_bases
ATGCAACGCAAAGGTGACACTCCCGCCACTCTGGACCTGTTCGCGGACCAGACGGCCCAGCCTCGCAGCGATGAGCGGAT
CGGGCCCGGGTCATGGCTGTTCAGAGGCTTTGCACTGCCGGCCATGCCGCAGCTGCTGCCTGCGCTTGAAGAGACACTGG
GCCTCTCCCCGTTCCGGCACATGCAGACCCCCAGCGGTCTGAGCATGTCTGCAGCACTGAGCAGTTGCGGACAACTGGGC
TGGATAACCGACCGCCACGGTTACCGGTACAGCGCGACCGACCCACAAACAGGACAGGCCTGGCCGACGATGCCGGACGT
ATTCATGCAGCTGGCGCAGGATGCCGCACTGGCAGCCGGCTATGCCGGGTTCGTGCCTGATGCCTGCCTGATCAATCGCT
ATATTCCGGGCGCCAAGATGTCCCTGCATCAGGACCGAGACGAATACGACCATCGCTGGCCAGTGGTCTCCGTATCACTG
GGCATACCGGCCATCTTTCAGTTCGGCGGCCTGCTGCGCGGCGACAAGACTCAACGCATCTCCCTGTTTCATGGCGACGT
GGTGGTCTGGGGTGGCGAGGACAGATTGCGTTTCCACGGCATTTTGCCCATCAAGCAGGCCGAGCATCCGCAGTTGGGTG
AGCAACGGATCAACCTGACCTTCCGCAAGGCTGGCCGCGACAGCTGA

Upstream 100 bases:

>100_bases
CGAGGCCGCCTTGAAGAAAATGCACGGCGAGAGCGACGACCTTATTACCGAGGTCAAGGTCAGCAAGGCTCTCTGATACT
CACCGGAGCTCGATCCGATC

Downstream 100 bases:

>100_bases
TCAGGCCTCTTCAAGCACACCGGAAGTACGGGCCAGCGCCGGGCATACTGCCCGGTGCAGGTTCAGTGCAGTATTGATGA
TGCCGATGTGGCTGAACGCC

Product: 2OG-Fe(II) oxygenase family protein

Products: NA

Alternate protein names: Alkylated DNA repair protein AlkB [H]

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MQRKGDTPATLDLFADQTAQPRSDERIGPGSWLFRGFALPAMPQLLPALEETLGLSPFRHMQTPSGLSMSAALSSCGQLG
WITDRHGYRYSATDPQTGQAWPTMPDVFMQLAQDAALAAGYAGFVPDACLINRYIPGAKMSLHQDRDEYDHRWPVVSVSL
GIPAIFQFGGLLRGDKTQRISLFHGDVVVWGGEDRLRFHGILPIKQAEHPQLGEQRINLTFRKAGRDS

Sequences:

>Translated_228_residues
MQRKGDTPATLDLFADQTAQPRSDERIGPGSWLFRGFALPAMPQLLPALEETLGLSPFRHMQTPSGLSMSAALSSCGQLG
WITDRHGYRYSATDPQTGQAWPTMPDVFMQLAQDAALAAGYAGFVPDACLINRYIPGAKMSLHQDRDEYDHRWPVVSVSL
GIPAIFQFGGLLRGDKTQRISLFHGDVVVWGGEDRLRFHGILPIKQAEHPQLGEQRINLTFRKAGRDS
>Mature_228_residues
MQRKGDTPATLDLFADQTAQPRSDERIGPGSWLFRGFALPAMPQLLPALEETLGLSPFRHMQTPSGLSMSAALSSCGQLG
WITDRHGYRYSATDPQTGQAWPTMPDVFMQLAQDAALAAGYAGFVPDACLINRYIPGAKMSLHQDRDEYDHRWPVVSVSL
GIPAIFQFGGLLRGDKTQRISLFHGDVVVWGGEDRLRFHGILPIKQAEHPQLGEQRINLTFRKAGRDS

Specific function: Dioxygenase that repairs alkylated DNA and RNA containing 3-methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine. Has lower activity towards alkylated DNA containing ethenoadenine, and no detectable a

COG id: COG3145

COG function: function code L; Alkylated DNA repair protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe2OG dioxygenase domain [H]

Homologues:

Organism=Homo sapiens, GI87298840, Length=124, Percent_Identity=29.8387096774194, Blast_Score=66, Evalue=2e-11,
Organism=Escherichia coli, GI1788541, Length=216, Percent_Identity=57.8703703703704, Blast_Score=244, Evalue=4e-66,
Organism=Drosophila melanogaster, GI45555401, Length=98, Percent_Identity=37.7551020408163, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 25216; Mature: 25216

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRKGDTPATLDLFADQTAQPRSDERIGPGSWLFRGFALPAMPQLLPALEETLGLSPFRH
CCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHH
MQTPSGLSMSAALSSCGQLGWITDRHGYRYSATDPQTGQAWPTMPDVFMQLAQDAALAAG
CCCCCCCHHHHHHHHCCCCCCEECCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
YAGFVPDACLINRYIPGAKMSLHQDRDEYDHRWPVVSVSLGIPAIFQFGGLLRGDKTQRI
CHHCCCCHHHHHHCCCCCCCHHHCCCHHHCCCCCEEEEECCCHHHHHHCCCCCCCCCCEE
SLFHGDVVVWGGEDRLRFHGILPIKQAEHPQLGEQRINLTFRKAGRDS
EEEECCEEEECCCCCEEEEECCCCCCCCCCCCCCHHEEEEEECCCCCC
>Mature Secondary Structure
MQRKGDTPATLDLFADQTAQPRSDERIGPGSWLFRGFALPAMPQLLPALEETLGLSPFRH
CCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHH
MQTPSGLSMSAALSSCGQLGWITDRHGYRYSATDPQTGQAWPTMPDVFMQLAQDAALAAG
CCCCCCCHHHHHHHHCCCCCCEECCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
YAGFVPDACLINRYIPGAKMSLHQDRDEYDHRWPVVSVSLGIPAIFQFGGLLRGDKTQRI
CHHCCCCHHHHHHCCCCCCCHHHCCCHHHCCCCCEEEEECCCHHHHHHCCCCCCCCCCEE
SLFHGDVVVWGGEDRLRFHGILPIKQAEHPQLGEQRINLTFRKAGRDS
EEEECCEEEECCCCCEEEEECCCCCCCCCCCCCCHHEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 1904855 [H]