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The map label for this gene is ygiD [H]

Identifier: 66046184

GI number: 66046184

Start: 3541469

End: 3542302

Strand: Direct

Name: ygiD [H]

Synonym: Psyr_2953

Alternate gene names: 66046184

Gene position: 3541469-3542302 (Clockwise)

Preceding gene: 66046183

Following gene: 66046185

Centisome position: 58.12

GC content: 58.51

Gene sequence:

>834_bases
ATGAACACTTCATTCCCCCTGCTGTTTGTGTCTCATGGTGCTCCCACGTTCGCCATCGAGCCCGGCCTGGCAGGCCCGAG
ACTGGCCAGGCTCGGTCGTGAGCTGCCAAAACCTGATGCCATCGTGGTTGTCTCGCCGCACTGGATGACCCGTGGCGAAG
TCAGGGTTACGTCGAGCACAGCACCTGAAACCATTCATGACTTTGGTGGTTTTCCGGGCGCTTTGTATACGTTGTCCTAC
CCGGCTCCGGGCGCACCAGCGTTAGCGAGTCATATTGTTGAGCTGCTGCAGACGGCGGGCTGGCAATCGCGGCTCGATTC
CGCCCGCGGCCTGGATCACGGTGCCTGGATACCACTTATGTATCTGGCACCCGACGCAGACATACCGGTCGTTCAGGTGT
CCATGCCCGCCACGCTTGATACACGCAGCGCCTGGCAGCTCGGCGCAGCACTCAAGCCACTGCGTGAGATGAACGTATTG
ATCGTGGCGTCAGGCAGCCTCACCCACAACCTGTACGAGTTCCGCGGCGCAACAAGCCACGGCGCCGACTACGTCAAACA
GTTCGCAGCATGGACCGCCCTGACCCTGCAGGCAGGAAACATTGATCAGTTGCTCGACTACAAGCAGTACGCACCTTCAG
CCGAACGTGCACATCCGACCGATGAGCATTTCCTGCCGCTGGTTATTGCGCTCGGGGCAGCTGGTCACGGTTATCAGACA
CAGGTAATGGACGGCGGTATTACCCACGGCGTTCTGGCGATGGACAGCTACCTGTTTACCTCAATTGATAGCGAACGATT
CGAGTCAGCTCAAACCGTTCAAGGTGTGAAATAA

Upstream 100 bases:

>100_bases
TTTCAACATCTACCTCGGCTACGGCGCGGGTCTGGGCACTTCGGTCGCTCCGGTCTGGCTGAGCAAGTAAGCAACACATC
ATCAATCAGGAGCAGTCACC

Downstream 100 bases:

>100_bases
CCTCATGCAAAACATCAACTTCTCTGAACCCGCACGCGAGTCCGAGTCGGGTATTTTTTTCCGTAAGGCATGTAGCACTG
TCAACCCAAAGGCACGGTTG

Product: catalytic LigB subunit of aromatic ring-opening dioxygenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MNTSFPLLFVSHGAPTFAIEPGLAGPRLARLGRELPKPDAIVVVSPHWMTRGEVRVTSSTAPETIHDFGGFPGALYTLSY
PAPGAPALASHIVELLQTAGWQSRLDSARGLDHGAWIPLMYLAPDADIPVVQVSMPATLDTRSAWQLGAALKPLREMNVL
IVASGSLTHNLYEFRGATSHGADYVKQFAAWTALTLQAGNIDQLLDYKQYAPSAERAHPTDEHFLPLVIALGAAGHGYQT
QVMDGGITHGVLAMDSYLFTSIDSERFESAQTVQGVK

Sequences:

>Translated_277_residues
MNTSFPLLFVSHGAPTFAIEPGLAGPRLARLGRELPKPDAIVVVSPHWMTRGEVRVTSSTAPETIHDFGGFPGALYTLSY
PAPGAPALASHIVELLQTAGWQSRLDSARGLDHGAWIPLMYLAPDADIPVVQVSMPATLDTRSAWQLGAALKPLREMNVL
IVASGSLTHNLYEFRGATSHGADYVKQFAAWTALTLQAGNIDQLLDYKQYAPSAERAHPTDEHFLPLVIALGAAGHGYQT
QVMDGGITHGVLAMDSYLFTSIDSERFESAQTVQGVK
>Mature_277_residues
MNTSFPLLFVSHGAPTFAIEPGLAGPRLARLGRELPKPDAIVVVSPHWMTRGEVRVTSSTAPETIHDFGGFPGALYTLSY
PAPGAPALASHIVELLQTAGWQSRLDSARGLDHGAWIPLMYLAPDADIPVVQVSMPATLDTRSAWQLGAALKPLREMNVL
IVASGSLTHNLYEFRGATSHGADYVKQFAAWTALTLQAGNIDQLLDYKQYAPSAERAHPTDEHFLPLVIALGAAGHGYQT
QVMDGGITHGVLAMDSYLFTSIDSERFESAQTVQGVK

Specific function: Unknown

COG id: COG3384

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DODA-type extradiol aromatic ring- opening dioxygenase family [H]

Homologues:

Organism=Escherichia coli, GI226510974, Length=269, Percent_Identity=40.8921933085502, Blast_Score=163, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014436
- InterPro:   IPR004183 [H]

Pfam domain/function: PF02900 LigB [H]

EC number: NA

Molecular weight: Translated: 29769; Mature: 29769

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTSFPLLFVSHGAPTFAIEPGLAGPRLARLGRELPKPDAIVVVSPHWMTRGEVRVTSST
CCCCCCEEEEECCCCEEEECCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEECCC
APETIHDFGGFPGALYTLSYPAPGAPALASHIVELLQTAGWQSRLDSARGLDHGAWIPLM
CCHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEE
YLAPDADIPVVQVSMPATLDTRSAWQLGAALKPLREMNVLIVASGSLTHNLYEFRGATSH
EECCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHCCCCCC
GADYVKQFAAWTALTLQAGNIDQLLDYKQYAPSAERAHPTDEHFLPLVIALGAAGHGYQT
CHHHHHHHHHHHEEEEECCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHEECCCCCCCEE
QVMDGGITHGVLAMDSYLFTSIDSERFESAQTVQGVK
EEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MNTSFPLLFVSHGAPTFAIEPGLAGPRLARLGRELPKPDAIVVVSPHWMTRGEVRVTSST
CCCCCCEEEEECCCCEEEECCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEECCC
APETIHDFGGFPGALYTLSYPAPGAPALASHIVELLQTAGWQSRLDSARGLDHGAWIPLM
CCHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEE
YLAPDADIPVVQVSMPATLDTRSAWQLGAALKPLREMNVLIVASGSLTHNLYEFRGATSH
EECCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHCCCCCC
GADYVKQFAAWTALTLQAGNIDQLLDYKQYAPSAERAHPTDEHFLPLVIALGAAGHGYQT
CHHHHHHHHHHHEEEEECCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHEECCCCCCCEE
QVMDGGITHGVLAMDSYLFTSIDSERFESAQTVQGVK
EEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1314093; 9278503 [H]