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The map label for this gene is pilT [H]

Identifier: 66046137

GI number: 66046137

Start: 3485827

End: 3486996

Strand: Reverse

Name: pilT [H]

Synonym: Psyr_2906

Alternate gene names: 66046137

Gene position: 3486996-3485827 (Counterclockwise)

Preceding gene: 66046139

Following gene: 66046129

Centisome position: 57.22

GC content: 61.11

Gene sequence:

>1170_bases
ATGGATTTCCCGGCGCTGTTGAAGATTCTGGCCAGTCAGGACGGATCGGACCTGTACCTCTCCACCGGAGCGCCACCGTG
CGCCAAGTTCAACGGTGTGCTCAAGCCACTGGGCAGCGAAACCTTCAAGCCGGGGGAGGTCGCAGTCATTGCCCAGGGGC
TGATGGACGAAGAACAGAAGCTCGAGTTTCAACGCGAACTGGAGATGAACCTCGCCGTTTCGATGGCGGGCATCGGCCGG
TTCCGGATCAATATCTTTATGCAGCGCAACGAAGTGTCCATCGTCGCGCGGAATATCAAACTGGACATCCCGCGTTTCGA
AGACCTGTTCCTGCCGCCCGTGCTGCTCGACGTGATCATGGAAAAGCGCGGTCTGGTGCTGTTTGTCGGGGCCACGGGTT
CGGGCAAGTCGACCTCATTGGCGGCGCTGATCGACTACCGTAACCGCAACGCCAGCGGCCATATCATCACCATTGAGGAC
CCGGTCGAGTTCATCCATCGCCACAAGAAATCGATCGTCAACCAGCGCGAAGTCGGGGTCGATACCCGCAGCTTCCATGC
GGCATTGAAAAACACGCTGCGCCAGGCGCCGGATGTGATTCTGATCGGCGAGATTCGTGATCGCGAGACCATGGAGCACG
CGCTGGCGTTTGCCGACACCGGCCACCTGGCGATCTCGACCCTGCACGCCAACAACGCCAATCAGGCGCTGGACCGGATC
ATCAATTTCTTCCCGGAAGAGCGTCGCGCGCAGTTGCTGCATGACCTGGGCAACAACCTCAAGGCATTCGTCTCCCAGCG
ACTGGTCAGAACACCGGATGGCAAGCGCCGCGCGGCGGTGGAAGTGATGATGGGCACACCGACCATTCGCGACCTGATCC
AGCGCAATGAACTGACCGAACTCAAGGGCATCATGGAGAAGTCCGGCAGCCTCGGTATGCAGACGTTCGACTCTGCGCTA
TTCAATCTGGCCGTCGAAGGGGCGATCAGCGAAGAAGAAGCCCTCAAGAACGCCGACTCGCAGAACAACGTGCGCCTGCG
CCTCAAACTGCACAGTGAAGGCGGCGCCGGGAGCCTCACCACACCGCCGCCGGCCCCGACCGGCAGCAGCACGGCAAGCA
CCGCAGAGTGGGGGCTGGTCGATGATGACGCGCCGGGTCCGCAGGCGTAA

Upstream 100 bases:

>100_bases
AGGCTGCCATTATGCCTGCTCTTGCTGAGCGACGCCCGCGCGTCACACGTGCTGTCCTGACGGTCGTGGTCTCTTTACTT
CATTTTGTACGGTATTGATT

Downstream 100 bases:

>100_bases
ATCGGTGACGCTACGCCCCCCCGGGCAGAAGCTTCGTTGTGGGCCAGATCATTCCGGATGCGCGGCCGCCAGCAATTCCT
GCGTGTAGGGATGTTGCGGT

Product: pilus retraction protein PilT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLGSETFKPGEVAVIAQGLMDEEQKLEFQRELEMNLAVSMAGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEDLFLPPVLLDVIMEKRGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVRTPDGKRRAAVEVMMGTPTIRDLIQRNELTELKGIMEKSGSLGMQTFDSAL
FNLAVEGAISEEEALKNADSQNNVRLRLKLHSEGGAGSLTTPPPAPTGSSTASTAEWGLVDDDAPGPQA

Sequences:

>Translated_389_residues
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLGSETFKPGEVAVIAQGLMDEEQKLEFQRELEMNLAVSMAGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEDLFLPPVLLDVIMEKRGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVRTPDGKRRAAVEVMMGTPTIRDLIQRNELTELKGIMEKSGSLGMQTFDSAL
FNLAVEGAISEEEALKNADSQNNVRLRLKLHSEGGAGSLTTPPPAPTGSSTASTAEWGLVDDDAPGPQA
>Mature_389_residues
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLGSETFKPGEVAVIAQGLMDEEQKLEFQRELEMNLAVSMAGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEDLFLPPVLLDVIMEKRGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVRTPDGKRRAAVEVMMGTPTIRDLIQRNELTELKGIMEKSGSLGMQTFDSAL
FNLAVEGAISEEEALKNADSQNNVRLRLKLHSEGGAGSLTTPPPAPTGSSTASTAEWGLVDDDAPGPQA

Specific function: May be involved in pilus retraction [H]

COG id: COG5008

COG function: function code NU; Tfp pilus assembly protein, ATPase PilU

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=327, Percent_Identity=33.3333333333333, Blast_Score=208, Evalue=4e-55,
Organism=Escherichia coli, GI1789723, Length=157, Percent_Identity=40.7643312101911, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1786296, Length=366, Percent_Identity=30.8743169398907, Blast_Score=96, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 42578; Mature: 42578

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLGSETFKPGEVAVIAQGLMDEEQK
CCHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCEEEEEHHHCCHHHH
LEFQRELEMNLAVSMAGIGRFRINIFMQRNEVSIVARNIKLDIPRFEDLFLPPVLLDVIM
HHHHHHHHHHHEEEEECCCEEEEEEEEECCCEEEEEEEEEECCCCHHHHCCCHHHHHHHH
EKRGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIEDPVEFIHRHKKSIVNQREVGV
HCCCEEEEEECCCCCCCCEEEEEEEECCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCC
DTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
CHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHH
INFFPEERRAQLLHDLGNNLKAFVSQRLVRTPDGKRRAAVEVMMGTPTIRDLIQRNELTE
HHHCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHCHHHH
LKGIMEKSGSLGMQTFDSALFNLAVEGAISEEEALKNADSQNNVRLRLKLHSEGGAGSLT
HHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCEEEEEEEECCCCCCCCC
TPPPAPTGSSTASTAEWGLVDDDAPGPQA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLGSETFKPGEVAVIAQGLMDEEQK
CCHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCEEEEEHHHCCHHHH
LEFQRELEMNLAVSMAGIGRFRINIFMQRNEVSIVARNIKLDIPRFEDLFLPPVLLDVIM
HHHHHHHHHHHEEEEECCCEEEEEEEEECCCEEEEEEEEEECCCCHHHHCCCHHHHHHHH
EKRGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIEDPVEFIHRHKKSIVNQREVGV
HCCCEEEEEECCCCCCCCEEEEEEEECCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCC
DTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
CHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHH
INFFPEERRAQLLHDLGNNLKAFVSQRLVRTPDGKRRAAVEVMMGTPTIRDLIQRNELTE
HHHCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHCHHHH
LKGIMEKSGSLGMQTFDSALFNLAVEGAISEEEALKNADSQNNVRLRLKLHSEGGAGSLT
HHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCEEEEEEEECCCCCCCCC
TPPPAPTGSSTASTAEWGLVDDDAPGPQA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]