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The map label for this gene is gacA [H]

Identifier: 66046128

GI number: 66046128

Start: 3476311

End: 3476979

Strand: Reverse

Name: gacA [H]

Synonym: Psyr_2897

Alternate gene names: 66046128

Gene position: 3476979-3476311 (Counterclockwise)

Preceding gene: 66046129

Following gene: 66046127

Centisome position: 57.06

GC content: 56.65

Gene sequence:

>669_bases
ATGACCGTTGCGCGAGGTGTCTGCTTGATTAAGGTGCTAGTTGTCGATGACCATGATCTTGTCCGGACAGGCATCACACG
CATGCTGGCCGACATTGATGGTCTGCAGGTTGTCGGACAGGCCGATTCCGGCGAGGAATCCCTGAAAAAAGCGCGCGAGC
TCAAGCCCGACGTCGTGCTGATGGACGTCAAGATGCCGGGTATCGGAGGTCTGGAAGCGACGCGCAAGATGCTGCGCAGC
CACCCGGATATCAAGGTCGTCGCTGTCACCGTCTGTGAAGAAGATCCTTTCCCGACCCGTTTGCTGCAGGCCGGCGCTGC
CGGTTACATGACCAAGGGCGCCGGGCTCGCTGAAATGGTCCAGGCCATTCGCCTGGTGTTTGCCGGTCAGCGTTACATCA
GCCCGCAGATTGCCCAGCAACTGGCATTGAAGTCATTTCAGCCGCAGGTCAACAATTCGCCGTTCGATCTGCTGTCCGAG
CGCGAGATCCAGATTGCCCTGATGATTGTTGGCTGTCAGAAAGTCCAGACCATCTCGGACAAGCTGTGCCTGTCACCGAA
AACCGTTAATACCTACCGATACCGTATCTTTGAAAAGCTCTCGATCAGCAGTGATGTTGAACTGGCTTTGCTCGCTGTAC
GTCACGGCATGGTTGATGCCAGCGCCTGA

Upstream 100 bases:

>100_bases
TGCAGCCCTCCCGGATGCGCCTGCCATCATATTGATGTGAGACGGTGTTCGCAGTGCTAGGTCTGCCTCGTTGTTTTTGT
TATGGTGATTGGCATTCGTT

Downstream 100 bases:

>100_bases
ACATGACCCAGACATTTGATCCAGGTGCATTTCTCGCGACCTGTAGCGGTCGCCCCGGCGTCTACCGTATGTTCGACGCC
GAAGCCACACTGCTTTACGT

Product: LuxR response regulator receiver

Products: NA

Alternate protein names: Global activator [H]

Number of amino acids: Translated: 222; Mature: 221

Protein sequence:

>222_residues
MTVARGVCLIKVLVVDDHDLVRTGITRMLADIDGLQVVGQADSGEESLKKARELKPDVVLMDVKMPGIGGLEATRKMLRS
HPDIKVVAVTVCEEDPFPTRLLQAGAAGYMTKGAGLAEMVQAIRLVFAGQRYISPQIAQQLALKSFQPQVNNSPFDLLSE
REIQIALMIVGCQKVQTISDKLCLSPKTVNTYRYRIFEKLSISSDVELALLAVRHGMVDASA

Sequences:

>Translated_222_residues
MTVARGVCLIKVLVVDDHDLVRTGITRMLADIDGLQVVGQADSGEESLKKARELKPDVVLMDVKMPGIGGLEATRKMLRS
HPDIKVVAVTVCEEDPFPTRLLQAGAAGYMTKGAGLAEMVQAIRLVFAGQRYISPQIAQQLALKSFQPQVNNSPFDLLSE
REIQIALMIVGCQKVQTISDKLCLSPKTVNTYRYRIFEKLSISSDVELALLAVRHGMVDASA
>Mature_221_residues
TVARGVCLIKVLVVDDHDLVRTGITRMLADIDGLQVVGQADSGEESLKKARELKPDVVLMDVKMPGIGGLEATRKMLRSH
PDIKVVAVTVCEEDPFPTRLLQAGAAGYMTKGAGLAEMVQAIRLVFAGQRYISPQIAQQLALKSFQPQVNNSPFDLLSER
EIQIALMIVGCQKVQTISDKLCLSPKTVNTYRYRIFEKLSISSDVELALLAVRHGMVDASA

Specific function: Forms part of a two-component regulatory system gacA/gacA(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=212, Percent_Identity=58.4905660377358, Blast_Score=260, Evalue=6e-71,
Organism=Escherichia coli, GI1790102, Length=201, Percent_Identity=33.3333333333333, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1786747, Length=197, Percent_Identity=27.9187817258883, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788521, Length=202, Percent_Identity=28.2178217821782, Blast_Score=88, Evalue=4e-19,
Organism=Escherichia coli, GI1788712, Length=207, Percent_Identity=26.0869565217391, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1787473, Length=202, Percent_Identity=27.7227722772277, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 24235; Mature: 24104

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVARGVCLIKVLVVDDHDLVRTGITRMLADIDGLQVVGQADSGEESLKKARELKPDVVL
CCCCCCEEEEEEEEECCCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCEEE
MDVKMPGIGGLEATRKMLRSHPDIKVVAVTVCEEDPFPTRLLQAGAAGYMTKGAGLAEMV
EEECCCCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHCCCCCCCCCCCHHHHH
QAIRLVFAGQRYISPQIAQQLALKSFQPQVNNSPFDLLSEREIQIALMIVGCQKVQTISD
HHHHHHHHCCHHCCHHHHHHHHHHHCCCCCCCCCHHHHCCCCEEEEEEEECCHHHHHHHH
KLCLSPKTVNTYRYRIFEKLSISSDVELALLAVRHGMVDASA
HHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TVARGVCLIKVLVVDDHDLVRTGITRMLADIDGLQVVGQADSGEESLKKARELKPDVVL
CCCCCEEEEEEEEECCCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCEEE
MDVKMPGIGGLEATRKMLRSHPDIKVVAVTVCEEDPFPTRLLQAGAAGYMTKGAGLAEMV
EEECCCCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHCCCCCCCCCCCHHHHH
QAIRLVFAGQRYISPQIAQQLALKSFQPQVNNSPFDLLSEREIQIALMIVGCQKVQTISD
HHHHHHHHCCHHCCHHHHHHHHHHHCCCCCCCCCHHHHCCCCEEEEEEEECCHHHHHHHH
KLCLSPKTVNTYRYRIFEKLSISSDVELALLAVRHGMVDASA
HHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8002569 [H]