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The map label for this gene is estB [H]

Identifier: 66045977

GI number: 66045977

Start: 3326933

End: 3328123

Strand: Reverse

Name: estB [H]

Synonym: Psyr_2741

Alternate gene names: 66045977

Gene position: 3328123-3326933 (Counterclockwise)

Preceding gene: 66045978

Following gene: 66045975

Centisome position: 54.62

GC content: 65.16

Gene sequence:

>1191_bases
ATGGCTGTCCTGAACGAGGCGGGGATACAGCGTCTTGACCATGTCTGGCGCAGTTTCGTCGAGCAGGGTCGGATCGTCGG
TGGCGTGCTGTTGCTGGCTCGGCATGGGCGTCTGGAATACGCCAGTGCGCGCGGCTGGGCCGATCGTGAACAGCAGATTC
CGGTCACCCGCCGCACGCGTTTTCGTCTGGCCTCACTGACCAAGCTGTTGACCTCGGTCGCGGTGTTGCGGCTCTGCGAA
CGGGGTGTGCTGGATCTGCACGCTTCGGTGACGCACTGGCTGCCAGCCTTTCGACCGCATCTGGCCAATGGCCGCGAGCC
GCTCATCACGCTGCAGCACCTGCTGTCACACGCGTCGGGCTTGAGCTACGGCTTTGAGCAAGCGCCCGATAACGCTTATC
AGCGCGCTGGGGTGTCGGACGGGCTGGATCGCGTACCGTTCGACCTGCAGGAAAACCTGAGGCGTTTGGCGCGTGTGCCC
TTGCTGTTTGAGCCCGGCAGCGCCTGGGGGTACTCGCTGGCCACGGATGTATTGGGTGCAGTGATCGAACAGGCCACGGG
GCTGGCGTTGTCAGAAGCCATCGATAAATGGGTCACCGTGCCTCTGCGCATGCATGCGACCTCATTCAGGCACCTGCCGG
AATGTCCGCTGGCCAGCGCCTACAAGGATGGGGTCAGTGGCCCGCAGCGTATCGGCGATGACGATGGGGTGTTGCTCGAC
AGCGGCCGGGCGCGGCTGTCCGCAGCCCGCGCCTTTGACTCATGCGCGTATGAGTCGGGCGGGGCGGGAATGCTCGGCAC
GGCTGATGACTATCTGCGGCTGCTCGAATGCCTGCGTCTCGGCGGCGCGCCGCTATTGAGTGAAGCCGGTACCGCACGCT
TGCTGGGCAATGCGATCGGCCAGACGATCATCGCCAGCCGCGGGCCCGGCTGGAGGTTCGGGCTGGGGCCGATGATCCTT
ATCGATCCAGTGCTTGCCGGGCAGCGGCAGGGGGCGGGAACCTGGTCGTGGTGCGGCCTGTATGGCAGTCATTATTGGGT
CGATCCGCGTGCTGCGCTGAGCATGGTGGTCCTGACCAACACGGCGGTTGCCGGCGCGTGGGGCGAGTTTGCCGACGCAA
TCGTCGATGCGCTTTACCCCAAGCTGGGGGTCAGGCCCGGGCAAATTGCTTGCGGTAGCTGCTCGGGCTGA

Upstream 100 bases:

>100_bases
GATGGCTTTCGCCGATGGCTGCTCGCACACCAACCCGATTCGCCATTCGGCGCAGGCCTACGCGCAGATCTTCAGCCGTG
CAGTGAGGGGCGAGTGAACC

Downstream 100 bases:

>100_bases
TGCCCACCAGATTGCGGAAGTGCCGGCGAAACGACTCTTCGGAGCCGAAGCCGGCGCGGTCCACCACTTCATTCAGGCGC
AATTTCGACGATTCCAGCAG

Product: Beta-lactamase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAVLNEAGIQRLDHVWRSFVEQGRIVGGVLLLARHGRLEYASARGWADREQQIPVTRRTRFRLASLTKLLTSVAVLRLCE
RGVLDLHASVTHWLPAFRPHLANGREPLITLQHLLSHASGLSYGFEQAPDNAYQRAGVSDGLDRVPFDLQENLRRLARVP
LLFEPGSAWGYSLATDVLGAVIEQATGLALSEAIDKWVTVPLRMHATSFRHLPECPLASAYKDGVSGPQRIGDDDGVLLD
SGRARLSAARAFDSCAYESGGAGMLGTADDYLRLLECLRLGGAPLLSEAGTARLLGNAIGQTIIASRGPGWRFGLGPMIL
IDPVLAGQRQGAGTWSWCGLYGSHYWVDPRAALSMVVLTNTAVAGAWGEFADAIVDALYPKLGVRPGQIACGSCSG

Sequences:

>Translated_396_residues
MAVLNEAGIQRLDHVWRSFVEQGRIVGGVLLLARHGRLEYASARGWADREQQIPVTRRTRFRLASLTKLLTSVAVLRLCE
RGVLDLHASVTHWLPAFRPHLANGREPLITLQHLLSHASGLSYGFEQAPDNAYQRAGVSDGLDRVPFDLQENLRRLARVP
LLFEPGSAWGYSLATDVLGAVIEQATGLALSEAIDKWVTVPLRMHATSFRHLPECPLASAYKDGVSGPQRIGDDDGVLLD
SGRARLSAARAFDSCAYESGGAGMLGTADDYLRLLECLRLGGAPLLSEAGTARLLGNAIGQTIIASRGPGWRFGLGPMIL
IDPVLAGQRQGAGTWSWCGLYGSHYWVDPRAALSMVVLTNTAVAGAWGEFADAIVDALYPKLGVRPGQIACGSCSG
>Mature_395_residues
AVLNEAGIQRLDHVWRSFVEQGRIVGGVLLLARHGRLEYASARGWADREQQIPVTRRTRFRLASLTKLLTSVAVLRLCER
GVLDLHASVTHWLPAFRPHLANGREPLITLQHLLSHASGLSYGFEQAPDNAYQRAGVSDGLDRVPFDLQENLRRLARVPL
LFEPGSAWGYSLATDVLGAVIEQATGLALSEAIDKWVTVPLRMHATSFRHLPECPLASAYKDGVSGPQRIGDDDGVLLDS
GRARLSAARAFDSCAYESGGAGMLGTADDYLRLLECLRLGGAPLLSEAGTARLLGNAIGQTIIASRGPGWRFGLGPMILI
DPVLAGQRQGAGTWSWCGLYGSHYWVDPRAALSMVVLTNTAVAGAWGEFADAIVDALYPKLGVRPGQIACGSCSG

Specific function: Acts on short-chain (C4-C6) fatty acid esters and triglycerides, including tertiary alcohol esters. Activity on p- nitrophenyl esters is generally higher than on o-nitrophenyl esters. Lacks beta-lactamase activity; it hydrolyzes the ester bond of cephalos

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-A beta-lactamase family [H]

Homologues:

Organism=Escherichia coli, GI87082101, Length=384, Percent_Identity=25.78125, Blast_Score=72, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17550934, Length=203, Percent_Identity=27.5862068965517, Blast_Score=72, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466
- InterPro:   IPR000871 [H]

Pfam domain/function: PF00144 Beta-lactamase [H]

EC number: NA

Molecular weight: Translated: 42609; Mature: 42478

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVLNEAGIQRLDHVWRSFVEQGRIVGGVLLLARHGRLEYASARGWADREQQIPVTRRTR
CCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEHHCCCCCCHHHCCCCHHHHH
FRLASLTKLLTSVAVLRLCERGVLDLHASVTHWLPAFRPHLANGREPLITLQHLLSHASG
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
LSYGFEQAPDNAYQRAGVSDGLDRVPFDLQENLRRLARVPLLFEPGSAWGYSLATDVLGA
CCCCCCCCCCHHHHHCCCCCCHHCCCHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHHH
VIEQATGLALSEAIDKWVTVPLRMHATSFRHLPECPLASAYKDGVSGPQRIGDDDGVLLD
HHHHHHCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHCCCCCCEEEC
SGRARLSAARAFDSCAYESGGAGMLGTADDYLRLLECLRLGGAPLLSEAGTARLLGNAIG
CCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHH
QTIIASRGPGWRFGLGPMILIDPVLAGQRQGAGTWSWCGLYGSHYWVDPRAALSMVVLTN
HHHHHCCCCCCEECCCCHHEECHHHHCCCCCCCCCHHHHHCCCCEEECHHHHHHHHHHHC
TAVAGAWGEFADAIVDALYPKLGVRPGQIACGSCSG
CHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
AVLNEAGIQRLDHVWRSFVEQGRIVGGVLLLARHGRLEYASARGWADREQQIPVTRRTR
CCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEHHCCCCCCHHHCCCCHHHHH
FRLASLTKLLTSVAVLRLCERGVLDLHASVTHWLPAFRPHLANGREPLITLQHLLSHASG
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
LSYGFEQAPDNAYQRAGVSDGLDRVPFDLQENLRRLARVPLLFEPGSAWGYSLATDVLGA
CCCCCCCCCCHHHHHCCCCCCHHCCCHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHHH
VIEQATGLALSEAIDKWVTVPLRMHATSFRHLPECPLASAYKDGVSGPQRIGDDDGVLLD
HHHHHHCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHCCCCCCEEEC
SGRARLSAARAFDSCAYESGGAGMLGTADDYLRLLECLRLGGAPLLSEAGTARLLGNAIG
CCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHH
QTIIASRGPGWRFGLGPMILIDPVLAGQRQGAGTWSWCGLYGSHYWVDPRAALSMVVLTN
HHHHHCCCCCCEECCCCHHEECHHHHCCCCCCCCCHHHHHCCCCEEECHHHHHHHHHHHC
TAVAGAWGEFADAIVDALYPKLGVRPGQIACGSCSG
CHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11472796; 11847270 [H]