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The map label for this gene is gudD [H]

Identifier: 66045952

GI number: 66045952

Start: 3298441

End: 3299715

Strand: Direct

Name: gudD [H]

Synonym: Psyr_2716

Alternate gene names: 66045952

Gene position: 3298441-3299715 (Clockwise)

Preceding gene: 66045951

Following gene: 66045955

Centisome position: 54.13

GC content: 61.1

Gene sequence:

>1275_bases
TTGAAAATCATCCGTGTAACCGTGACCCCTATCGCCTTTCGCGACCCGCCGTTGCTCAACGCCAGTGGTATTCACGAACC
CTTTGCCCTGCGCTCGATCATCGAGGTGGAAAGCGACAACGGCTACATCGGCCTCGGTGAAAGCTATGGTGATGCCCCGG
CACTGGCGATTCAGCAACAGGTGCAGACCCAGCTGATCGGGCTCGACCCGTTCAACCTCAATCAGCTGCGCAGCATCGTG
CAGGCGACCGTGGCGGCGCATAAGCCTGCCAGCCTGGCCGGTGCCGAACTGGCGCCGGGCTCACACGCCAGCAAGGCGGT
CAGCAATGCTTATTCTGCGTTCGAAGTAGCGTTTCTGGACCTGCAGGCACGTTCCATGAACCTGCCACTGGTGGACCTGC
TCGGCGGTGCCATTCGTGACCAGATACCCTTCAGCGCCTATCTGTTCTTCAAATATGCGCAGCATGCCGATGCGCCTTAC
GCGCCCGACAGCTGGGGTGAAGCGCTCAGCGAAGAGCAGATCGTCGCCCAGGCCCGGCGCATGATCGACGCCTACGGTTT
CAAAAGCATCAAGCTCAAGGCCGGTGCACTGCAGCCGGAACACGAGGTGTCGTGCATCAAGGCGCTGAAAAAAGCGTTTC
CCGGTTACCCGCTGCGCATCGACCCCAACGGCAACTGGTCACTGGAGACCTCGATTCGGATGGCCGAACTGCTGGGCGAT
GATCTGCAATATTACGAAGACCCGACGCCCGGCCTCGACGGCATGTCCGAATTGCACAAACGCACCGGACTGCCGCTGGC
GACCAACATGGTGGTGACCGATTTCGACGAGTTCCGCCGCAGCGTGGCACTGGACAGCGTGCAGATCGTGCTCGCCGACC
ACCATTACTGGGGCGGCCTGCGCGACACTCAGGCGCTGGCGAAGATGTGCGAAACCTTTGGCCTGGGCGTGTCGATGCAC
TCCAACTCGCATCTGGGCATCAGCCTCATGGCCATGGCCCACGTCGCAGCGTCGGTGCCCAATCTGGACTACGCCTGCGA
CACGCACTACCCGTGGCAGGAACCGGACGAGGAAGTCATCAAGGGCGGCAAACTGCCGATTGTCGACGGCTGTGTCAGCA
TCACCCGCGCCCCTGGCATGGGGCTGGAACTGGACTACGACCAGCTCGGCAAGCTCAACGACCAGTACCACAGCTGTGGC
ATCCGGCAGCGCGATGATGTGAAGCAGATGCAGAAGTACAATCCGGACTGGAAGGCGGTCAAGCCAAGATACTGA

Upstream 100 bases:

>100_bases
CCGCGGCCGCCCGTCCAGGGCTGAGCGGCCCGCAGCGCTCAGCCCTGCCGTCAATGGCACCCCTTCCAGCCTCAAACCCT
GACCTCCGGGAGACCGCCCT

Downstream 100 bases:

>100_bases
GACCATCCGGCCCAGCTCAATCGGCTCGCTGAGCAGGAGCGGTCGTCTGGTAGACACCCGGTGGATCCTGCCGGGTGCGG
ATTGGTGATCTTGCTGGCAG

Product: mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: GDH; GlucD [H]

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MKIIRVTVTPIAFRDPPLLNASGIHEPFALRSIIEVESDNGYIGLGESYGDAPALAIQQQVQTQLIGLDPFNLNQLRSIV
QATVAAHKPASLAGAELAPGSHASKAVSNAYSAFEVAFLDLQARSMNLPLVDLLGGAIRDQIPFSAYLFFKYAQHADAPY
APDSWGEALSEEQIVAQARRMIDAYGFKSIKLKAGALQPEHEVSCIKALKKAFPGYPLRIDPNGNWSLETSIRMAELLGD
DLQYYEDPTPGLDGMSELHKRTGLPLATNMVVTDFDEFRRSVALDSVQIVLADHHYWGGLRDTQALAKMCETFGLGVSMH
SNSHLGISLMAMAHVAASVPNLDYACDTHYPWQEPDEEVIKGGKLPIVDGCVSITRAPGMGLELDYDQLGKLNDQYHSCG
IRQRDDVKQMQKYNPDWKAVKPRY

Sequences:

>Translated_424_residues
MKIIRVTVTPIAFRDPPLLNASGIHEPFALRSIIEVESDNGYIGLGESYGDAPALAIQQQVQTQLIGLDPFNLNQLRSIV
QATVAAHKPASLAGAELAPGSHASKAVSNAYSAFEVAFLDLQARSMNLPLVDLLGGAIRDQIPFSAYLFFKYAQHADAPY
APDSWGEALSEEQIVAQARRMIDAYGFKSIKLKAGALQPEHEVSCIKALKKAFPGYPLRIDPNGNWSLETSIRMAELLGD
DLQYYEDPTPGLDGMSELHKRTGLPLATNMVVTDFDEFRRSVALDSVQIVLADHHYWGGLRDTQALAKMCETFGLGVSMH
SNSHLGISLMAMAHVAASVPNLDYACDTHYPWQEPDEEVIKGGKLPIVDGCVSITRAPGMGLELDYDQLGKLNDQYHSCG
IRQRDDVKQMQKYNPDWKAVKPRY
>Mature_424_residues
MKIIRVTVTPIAFRDPPLLNASGIHEPFALRSIIEVESDNGYIGLGESYGDAPALAIQQQVQTQLIGLDPFNLNQLRSIV
QATVAAHKPASLAGAELAPGSHASKAVSNAYSAFEVAFLDLQARSMNLPLVDLLGGAIRDQIPFSAYLFFKYAQHADAPY
APDSWGEALSEEQIVAQARRMIDAYGFKSIKLKAGALQPEHEVSCIKALKKAFPGYPLRIDPNGNWSLETSIRMAELLGD
DLQYYEDPTPGLDGMSELHKRTGLPLATNMVVTDFDEFRRSVALDSVQIVLADHHYWGGLRDTQALAKMCETFGLGVSMH
SNSHLGISLMAMAHVAASVPNLDYACDTHYPWQEPDEEVIKGGKLPIVDGCVSITRAPGMGLELDYDQLGKLNDQYHSCG
IRQRDDVKQMQKYNPDWKAVKPRY

Specific function: Catalyzes the dehydration of glucarate to 5-keto-4- deoxy-D-glucarate (5-kdGluc) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GlucD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789150, Length=433, Percent_Identity=33.4872979214781, Blast_Score=227, Evalue=9e-61,
Organism=Escherichia coli, GI1789151, Length=443, Percent_Identity=34.0857787810384, Blast_Score=222, Evalue=3e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.40 [H]

Molecular weight: Translated: 46613; Mature: 46613

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIIRVTVTPIAFRDPPLLNASGIHEPFALRSIIEVESDNGYIGLGESYGDAPALAIQQQ
CEEEEEEECEEEECCCCCCCCCCCCCHHHHHHHHEEECCCCEEECCCCCCCCHHHHHHHH
VQTQLIGLDPFNLNQLRSIVQATVAAHKPASLAGAELAPGSHASKAVSNAYSAFEVAFLD
HHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEEEEE
LQARSMNLPLVDLLGGAIRDQIPFSAYLFFKYAQHADAPYAPDSWGEALSEEQIVAQARR
EEHHCCCCCHHHHHCHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
MIDAYGFKSIKLKAGALQPEHEVSCIKALKKAFPGYPLRIDPNGNWSLETSIRMAELLGD
HHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCEEEHHHHHHHHHHCC
DLQYYEDPTPGLDGMSELHKRTGLPLATNMVVTDFDEFRRSVALDSVQIVLADHHYWGGL
HHHHHCCCCCCCCHHHHHHHHCCCCEECCCEEECHHHHHHHHHHCCEEEEEECCCCCCCC
RDTQALAKMCETFGLGVSMHSNSHLGISLMAMAHVAASVPNLDYACDTHYPWQEPDEEVI
HHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHCCCCCCCEECCCCCCCCCCHHHH
KGGKLPIVDGCVSITRAPGMGLELDYDQLGKLNDQYHSCGIRQRDDVKQMQKYNPDWKAV
CCCCCCEEHHHHHHHCCCCCCCEECHHHHCCCCCHHHHCCCCCHHHHHHHHHCCCCCCCC
KPRY
CCCC
>Mature Secondary Structure
MKIIRVTVTPIAFRDPPLLNASGIHEPFALRSIIEVESDNGYIGLGESYGDAPALAIQQQ
CEEEEEEECEEEECCCCCCCCCCCCCHHHHHHHHEEECCCCEEECCCCCCCCHHHHHHHH
VQTQLIGLDPFNLNQLRSIVQATVAAHKPASLAGAELAPGSHASKAVSNAYSAFEVAFLD
HHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEEEEE
LQARSMNLPLVDLLGGAIRDQIPFSAYLFFKYAQHADAPYAPDSWGEALSEEQIVAQARR
EEHHCCCCCHHHHHCHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
MIDAYGFKSIKLKAGALQPEHEVSCIKALKKAFPGYPLRIDPNGNWSLETSIRMAELLGD
HHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCEEEHHHHHHHHHHCC
DLQYYEDPTPGLDGMSELHKRTGLPLATNMVVTDFDEFRRSVALDSVQIVLADHHYWGGL
HHHHHCCCCCCCCHHHHHHHHCCCCEECCCEEECHHHHHHHHHHCCEEEEEECCCCCCCC
RDTQALAKMCETFGLGVSMHSNSHLGISLMAMAHVAASVPNLDYACDTHYPWQEPDEEVI
HHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHCCCCCCCEECCCCCCCCCCHHHH
KGGKLPIVDGCVSITRAPGMGLELDYDQLGKLNDQYHSCGIRQRDDVKQMQKYNPDWKAV
CCCCCCEEHHHHHHHCCCCCCCEECHHHHCCCCCHHHHCCCCCHHHHHHHHHCCCCCCCC
KPRY
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]