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The map label for this gene is vanB [H]

Identifier: 66045945

GI number: 66045945

Start: 3290248

End: 3291198

Strand: Direct

Name: vanB [H]

Synonym: Psyr_2709

Alternate gene names: 66045945

Gene position: 3290248-3291198 (Clockwise)

Preceding gene: 66045944

Following gene: 66045951

Centisome position: 53.99

GC content: 61.2

Gene sequence:

>951_bases
ATGATCGACGTGACAGTGTTATCACGTAACGATGAAGCGCTGGATATCTGCAGCTTTGAACTGGTGCGGGCAGACGGAGC
GCTGTTGCCACCGTTCAGCGCAGGCGCGCACATTGACGTGCACCTGCCTGACGGGCTGATCCGCCAGTACTCGCTGTGCA
ATGCCCCGAGCGAGCGTCACCGCTATCTGATCGGCGTCCTCAACGACCCGGCCTCGCGTGGGGGCTCACGCAGCCTGCAT
CAGCAGATTCAGCCTGGCGCGCAGTTGCGCATCAGCGAACCGCGCAACCTGTTCCCGCTGGCTGAAAACAGCCGACGCAC
GCTGCTGTTCGCCGGCGGTATCGGCATCACCCCGATCCTGTGCATGGCCGAACAGCTTGCGCTGGAGGCTGCCGATTTCG
AGTTGCACTACTGCGTGCGTTCCGCCGAGCGCGGCGCCTTTATCGAGCGCCTGAAGCATTCGGCATTTGCCGAGCGCATC
ACGCTGCACTTCGATGAACAGCCGGACACCGCACTGGAGGCTGGTAAAGTGCTCGCCAACCCACAGCCCGATACCCATCT
GTACGTGTGCGGGCCAGGCGGTTTCATGCAATACATCCTCGACAGCGCGCGGGCCGCTGGCTGGTCCGAGGACACTCTGC
ACCGCGAGTACTTCTCCGCAGAGCCGGTAGACACCAGCAACGACGGCAGTTTTTCGATAGAAATCGCCAGTACCGGGCAG
GTCATTGCCATTCCGGCCAATAAAACCGTCGCTCAGGTGCTCGAAAGCCACGGCATCGATATTCCACTGTCGTGTGAGCA
AGGTGTCTGCGGCACCTGCCTGACGCGGGTGTTGAAAGGCATTCCGGATCATCGCGATCTGTTTCTGACCGAAGACGAGC
AGGCGCTCAACGATCAGTTCACGCCCTGCTGCTCGCGTTCGAAGACGCCCGTGCTGGTGCTCGATATCTGA

Upstream 100 bases:

>100_bases
CAGATTCCCGTGATGGCGACGAAATGACCGGCCATTGCCCGTCGAAATACGAAGAAAACAATCAGAAGTGACCCTTGCCG
CGGTCACCCGAGGACCTGAC

Downstream 100 bases:

>100_bases
TCAGTCCGGGCGCTGAATGACCTTCACGCGCTCGTTCTGCCCGGCGCCGAAGATTTCCGCATAGCGCAAGGTGGCGTTGG
CATGCTCGCGCATGATGGCC

Product: ferredoxin:oxidoreductase FAD/NAD(P)-binding:oxidoreductase FAD-binding region

Products: 3,4-dihydroxybenzoate; NAD+; H2O; formaldehyde

Alternate protein names: Vanillate degradation ferredoxin-like protein [H]

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MIDVTVLSRNDEALDICSFELVRADGALLPPFSAGAHIDVHLPDGLIRQYSLCNAPSERHRYLIGVLNDPASRGGSRSLH
QQIQPGAQLRISEPRNLFPLAENSRRTLLFAGGIGITPILCMAEQLALEAADFELHYCVRSAERGAFIERLKHSAFAERI
TLHFDEQPDTALEAGKVLANPQPDTHLYVCGPGGFMQYILDSARAAGWSEDTLHREYFSAEPVDTSNDGSFSIEIASTGQ
VIAIPANKTVAQVLESHGIDIPLSCEQGVCGTCLTRVLKGIPDHRDLFLTEDEQALNDQFTPCCSRSKTPVLVLDI

Sequences:

>Translated_316_residues
MIDVTVLSRNDEALDICSFELVRADGALLPPFSAGAHIDVHLPDGLIRQYSLCNAPSERHRYLIGVLNDPASRGGSRSLH
QQIQPGAQLRISEPRNLFPLAENSRRTLLFAGGIGITPILCMAEQLALEAADFELHYCVRSAERGAFIERLKHSAFAERI
TLHFDEQPDTALEAGKVLANPQPDTHLYVCGPGGFMQYILDSARAAGWSEDTLHREYFSAEPVDTSNDGSFSIEIASTGQ
VIAIPANKTVAQVLESHGIDIPLSCEQGVCGTCLTRVLKGIPDHRDLFLTEDEQALNDQFTPCCSRSKTPVLVLDI
>Mature_316_residues
MIDVTVLSRNDEALDICSFELVRADGALLPPFSAGAHIDVHLPDGLIRQYSLCNAPSERHRYLIGVLNDPASRGGSRSLH
QQIQPGAQLRISEPRNLFPLAENSRRTLLFAGGIGITPILCMAEQLALEAADFELHYCVRSAERGAFIERLKHSAFAERI
TLHFDEQPDTALEAGKVLANPQPDTHLYVCGPGGFMQYILDSARAAGWSEDTLHREYFSAEPVDTSNDGSFSIEIASTGQ
VIAIPANKTVAQVLESHGIDIPLSCEQGVCGTCLTRVLKGIPDHRDLFLTEDEQALNDQFTPCCSRSKTPVLVLDI

Specific function: Unknown

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788104, Length=320, Percent_Identity=38.4375, Blast_Score=215, Evalue=3e-57,
Organism=Escherichia coli, GI1787658, Length=286, Percent_Identity=27.972027972028, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1787098, Length=284, Percent_Identity=24.2957746478873, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1788903, Length=247, Percent_Identity=27.9352226720648, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR000951
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.14.13.82

Molecular weight: Translated: 34532; Mature: 34532

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDVTVLSRNDEALDICSFELVRADGALLPPFSAGAHIDVHLPDGLIRQYSLCNAPSERH
CEEEEEECCCCCEEEHHEEEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHHCCCCCCCC
RYLIGVLNDPASRGGSRSLHQQIQPGAQLRISEPRNLFPLAENSRRTLLFAGGIGITPIL
EEEEEEECCCCCCCCCHHHHHHCCCCCEEEECCCCCCCCCCCCCCEEEEEECCCCHHHHH
CMAEQLALEAADFELHYCVRSAERGAFIERLKHSAFAERITLHFDEQPDTALEAGKVLAN
HHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHEEEEEECCCCCHHHHCCCEECC
PQPDTHLYVCGPGGFMQYILDSARAAGWSEDTLHREYFSAEPVDTSNDGSFSIEIASTGQ
CCCCCEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCEEEEEEECCCC
VIAIPANKTVAQVLESHGIDIPLSCEQGVCGTCLTRVLKGIPDHRDLFLTEDEQALNDQF
EEEEECCHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHCCCCCCCEEEECCHHHHCCCC
TPCCSRSKTPVLVLDI
CHHHCCCCCCEEEEEC
>Mature Secondary Structure
MIDVTVLSRNDEALDICSFELVRADGALLPPFSAGAHIDVHLPDGLIRQYSLCNAPSERH
CEEEEEECCCCCEEEHHEEEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHHCCCCCCCC
RYLIGVLNDPASRGGSRSLHQQIQPGAQLRISEPRNLFPLAENSRRTLLFAGGIGITPIL
EEEEEEECCCCCCCCCHHHHHHCCCCCEEEECCCCCCCCCCCCCCEEEEEECCCCHHHHH
CMAEQLALEAADFELHYCVRSAERGAFIERLKHSAFAERITLHFDEQPDTALEAGKVLAN
HHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHEEEEEECCCCCHHHHCCCEECC
PQPDTHLYVCGPGGFMQYILDSARAAGWSEDTLHREYFSAEPVDTSNDGSFSIEIASTGQ
CCCCCEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCEEEEEEECCCC
VIAIPANKTVAQVLESHGIDIPLSCEQGVCGTCLTRVLKGIPDHRDLFLTEDEQALNDQF
EEEEECCHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHCCCCCCCEEEECCHHHHCCCC
TPCCSRSKTPVLVLDI
CHHHCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: vanillate; O2; NADH; H+

Specific reaction: vanillate + O2 + NADH + H+ = 3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579070 [H]