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The map label for this gene is puuR [H]

Identifier: 66045941

GI number: 66045941

Start: 3286524

End: 3287135

Strand: Reverse

Name: puuR [H]

Synonym: Psyr_2705

Alternate gene names: 66045941

Gene position: 3287135-3286524 (Counterclockwise)

Preceding gene: 66045942

Following gene: 66045939

Centisome position: 53.94

GC content: 58.5

Gene sequence:

>612_bases
ATGGACGATCCATTGCGCCGGGGCGCTGCCTCATTCACCGGATATCTTTCACATAAGGCCACTATGGATACGGGAGCACG
ACTCAAACTGGTGCGTGAAAGCTACAAGCTGTCACAGCGTGAGCTCGCCCGACGCAGTGGTGTGACCAATGCCACGATTT
CCCTTATCGAACAAAATCGTGTCAGTCCTTCCATCAGCTCGCTGAAAAAGCTGCTTGAGGGGATTCCCATGACGCTGGCG
GATTTTTTCACATTCGATCAGCCTCCGGGACAGGATCAGTACGTGTTTCGAGCCGGTGATCAACCCGATCTGGGGCGCAA
CGGTGCCCGTTTGCTGCTGGTGGGCGCGACCCTGCCAAGCCGGCAGATGCGTTTTCTGCGTGAGCAATACGCGCCGGGTG
CCGACTCGGGCGATGAGCCGATCGTTCACAGCGAAGGCGAGGAGTGCGGTCTGGTGACGCGCGGCACCGTGGAGCTGACC
ATCGATGGGCAAGTCAACATTCTCGGTCCGGGGGACGGCTATTATTTCCCGACCACCTTGCCGCATCGCTTCCGCAATAT
CGGCCATGACGAAGCCGAGATCATCAGCGCCAACACACCGGCCAACTTCTAG

Upstream 100 bases:

>100_bases
GAGGCCTGCCAGAGCTATGCGCGCAACCGCCGCTAGAGCAATGAGCTGCTGAGTGTTGCGGGCAAACGTGATTTAATATC
CGGCTTGACGGCTTCGGGTA

Downstream 100 bases:

>100_bases
CATGCGCTTGCTGGCAGGCGTCACGATAACGTGATGATCGTTCAGCACGTCTGGCAACACGTCTGGATAAAATTCAACTG
ACCAGCGCTGTCGGCCTGTA

Product: helix-hairpin-helix DNA-binding motif-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MDDPLRRGAASFTGYLSHKATMDTGARLKLVRESYKLSQRELARRSGVTNATISLIEQNRVSPSISSLKKLLEGIPMTLA
DFFTFDQPPGQDQYVFRAGDQPDLGRNGARLLLVGATLPSRQMRFLREQYAPGADSGDEPIVHSEGEECGLVTRGTVELT
IDGQVNILGPGDGYYFPTTLPHRFRNIGHDEAEIISANTPANF

Sequences:

>Translated_203_residues
MDDPLRRGAASFTGYLSHKATMDTGARLKLVRESYKLSQRELARRSGVTNATISLIEQNRVSPSISSLKKLLEGIPMTLA
DFFTFDQPPGQDQYVFRAGDQPDLGRNGARLLLVGATLPSRQMRFLREQYAPGADSGDEPIVHSEGEECGLVTRGTVELT
IDGQVNILGPGDGYYFPTTLPHRFRNIGHDEAEIISANTPANF
>Mature_203_residues
MDDPLRRGAASFTGYLSHKATMDTGARLKLVRESYKLSQRELARRSGVTNATISLIEQNRVSPSISSLKKLLEGIPMTLA
DFFTFDQPPGQDQYVFRAGDQPDLGRNGARLLLVGATLPSRQMRFLREQYAPGADSGDEPIVHSEGEECGLVTRGTVELT
IDGQVNILGPGDGYYFPTTLPHRFRNIGHDEAEIISANTPANF

Specific function: Represses puuD [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787557, Length=179, Percent_Identity=39.1061452513966, Blast_Score=125, Evalue=2e-30,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013096
- InterPro:   IPR011051
- InterPro:   IPR001387
- InterPro:   IPR010982
- InterPro:   IPR014710 [H]

Pfam domain/function: PF07883 Cupin_2; PF01381 HTH_3 [H]

EC number: NA

Molecular weight: Translated: 22296; Mature: 22296

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS50943 HTH_CROC1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDPLRRGAASFTGYLSHKATMDTGARLKLVRESYKLSQRELARRSGVTNATISLIEQNR
CCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC
VSPSISSLKKLLEGIPMTLADFFTFDQPPGQDQYVFRAGDQPDLGRNGARLLLVGATLPS
CCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCEEEEEECCCCH
RQMRFLREQYAPGADSGDEPIVHSEGEECGLVTRGTVELTIDGQVNILGPGDGYYFPTTL
HHHHHHHHHCCCCCCCCCCCCEECCCCCCCEEECCEEEEEECCEEEEEECCCCCCCCCCC
PHRFRNIGHDEAEIISANTPANF
HHHHHHCCCCCCEEEECCCCCCC
>Mature Secondary Structure
MDDPLRRGAASFTGYLSHKATMDTGARLKLVRESYKLSQRELARRSGVTNATISLIEQNR
CCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC
VSPSISSLKKLLEGIPMTLADFFTFDQPPGQDQYVFRAGDQPDLGRNGARLLLVGATLPS
CCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCEEEEEECCCCH
RQMRFLREQYAPGADSGDEPIVHSEGEECGLVTRGTVELTIDGQVNILGPGDGYYFPTTL
HHHHHHHHHCCCCCCCCCCCCEECCCCCCCEEECCEEEEEECCEEEEEECCCCCCCCCCC
PHRFRNIGHDEAEIISANTPANF
HHHHHHCCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553 [H]