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The map label for this gene is cheR [C]

Identifier: 66045682

GI number: 66045682

Start: 2833881

End: 2834723

Strand: Direct

Name: cheR [C]

Synonym: Psyr_2446

Alternate gene names: 66045682

Gene position: 2833881-2834723 (Clockwise)

Preceding gene: 66045681

Following gene: 66045683

Centisome position: 46.51

GC content: 55.16

Gene sequence:

>843_bases
ATGACAAGCCCGGACCGCACCATGCACCGTGATCGAAGCATGGATATCGAGCTGCGCCTGCTGATCGAGGCGATTTACCT
TCAATACAGCTACGACTTTCGTGATTACTCCGGCGCTTCGGTCAAGCGTCGCGTGATCCATGCGCTGCGTCAGTTCGATT
GTGCGACCATCTCCGCCCTGCAGGAACGTGTGCTCCACGATCCTGCAGCATTCATGCAACTGCTGCAGACTCTCACGATT
CCGGTCAGTGAGATGTTCCGTGACCCGTCGCATTTTCTGGCAATCCGCAACGAGGTCGTGCCGGTACTGCGCACCTACCC
ATCACTCAAGGTGTGGATTGCCGGTTGCAGCACGGGCGAGGAGGTCTATTCCACGGCTATTCTGTTGCGCGAGGAGGGGC
TGTTGGAGCGGACCATCATCTATGCGACCGATATCAACCCCAGCTCGCTGGAAAAGGCCAAGCAGGGCATCTTCTCCATG
GACAACGTGCGGGCCTATGCCGAGAACTATCGAAAGGCGGGAGGGCAGCGCGATTTTGCCGAGTACTACACGTCTGCCTA
TGACTATGCGATCTTCGACAAGACGCTGTGCGAGAACGTCACGTTTGCCGATCACAGTCTGGCCACAGATAGTGTATTCT
CCGAAACGCAATTTATTTCTTGTCGTAACGTACTGATTTATTTCAACAAAAAGCTTCAGGATCGAGCGTTCGGGCTGTTT
CATGATTCGTTGTGCCATCGTGGCTTTCTGGCGTTGGGCAGCAAGGAAACCCTGGATTTCTCCGCCTACGGTGGCGCCTT
CGAGGCGCTGGTGAAGCCCGAGCGGATTTACCGGAAATTATGA

Upstream 100 bases:

>100_bases
GCCAAGCCCATTGATCTGGACCGTCTGTTTTCGCTGATTCGCGTCTGGCTGCCGAAGATGGAGCGTATCTGAATGAGGGC
TCAATGTAGAGGCGTCTGCG

Downstream 100 bases:

>100_bases
AAATCACGTTTGGCGATGACACGCTCAGCCACCCGTTGCCCGTGGTCGATGCCATTGTCGTCGGCGCCTCGGCGGGCGGC
GTGGAGGCGCTGCTGAAAAT

Product: MCP methyltransferase, CheR-type:MCP methyltransferase, CheR-type

Products: S-adenosyl-L-homocysteine; protein L-glutamate methyl ester

Alternate protein names: ORF19 [H]

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MTSPDRTMHRDRSMDIELRLLIEAIYLQYSYDFRDYSGASVKRRVIHALRQFDCATISALQERVLHDPAAFMQLLQTLTI
PVSEMFRDPSHFLAIRNEVVPVLRTYPSLKVWIAGCSTGEEVYSTAILLREEGLLERTIIYATDINPSSLEKAKQGIFSM
DNVRAYAENYRKAGGQRDFAEYYTSAYDYAIFDKTLCENVTFADHSLATDSVFSETQFISCRNVLIYFNKKLQDRAFGLF
HDSLCHRGFLALGSKETLDFSAYGGAFEALVKPERIYRKL

Sequences:

>Translated_280_residues
MTSPDRTMHRDRSMDIELRLLIEAIYLQYSYDFRDYSGASVKRRVIHALRQFDCATISALQERVLHDPAAFMQLLQTLTI
PVSEMFRDPSHFLAIRNEVVPVLRTYPSLKVWIAGCSTGEEVYSTAILLREEGLLERTIIYATDINPSSLEKAKQGIFSM
DNVRAYAENYRKAGGQRDFAEYYTSAYDYAIFDKTLCENVTFADHSLATDSVFSETQFISCRNVLIYFNKKLQDRAFGLF
HDSLCHRGFLALGSKETLDFSAYGGAFEALVKPERIYRKL
>Mature_279_residues
TSPDRTMHRDRSMDIELRLLIEAIYLQYSYDFRDYSGASVKRRVIHALRQFDCATISALQERVLHDPAAFMQLLQTLTIP
VSEMFRDPSHFLAIRNEVVPVLRTYPSLKVWIAGCSTGEEVYSTAILLREEGLLERTIIYATDINPSSLEKAKQGIFSMD
NVRAYAENYRKAGGQRDFAEYYTSAYDYAIFDKTLCENVTFADHSLATDSVFSETQFISCRNVLIYFNKKLQDRAFGLFH
DSLCHRGFLALGSKETLDFSAYGGAFEALVKPERIYRKL

Specific function: Methylation Of The Membrane-Bound Methyl-Accepting Chemotaxis Proteins (Mcp) To Form Gamma-Glutamyl Methyl Ester Residues In Mcp. [C]

COG id: COG1352

COG function: function code NT; Methylase of chemotaxis methyl-accepting proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 cheR-type methyltransferase domain [H]

Homologues:

Organism=Escherichia coli, GI1788193, Length=270, Percent_Identity=26.6666666666667, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022642
- InterPro:   IPR000780
- InterPro:   IPR022641
- InterPro:   IPR000014
- InterPro:   IPR013656
- InterPro:   IPR000673 [H]

Pfam domain/function: PF01339 CheB_methylest; PF01739 CheR; PF03705 CheR_N; PF08448 PAS_4 [H]

EC number: 2.1.1.80

Molecular weight: Translated: 32139; Mature: 32008

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS50123 CHER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSPDRTMHRDRSMDIELRLLIEAIYLQYSYDFRDYSGASVKRRVIHALRQFDCATISAL
CCCCCCHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHH
QERVLHDPAAFMQLLQTLTIPVSEMFRDPSHFLAIRNEVVPVLRTYPSLKVWIAGCSTGE
HHHHHCCHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCH
EVYSTAILLREEGLLERTIIYATDINPSSLEKAKQGIFSMDNVRAYAENYRKAGGQRDFA
HHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
EYYTSAYDYAIFDKTLCENVTFADHSLATDSVFSETQFISCRNVLIYFNKKLQDRAFGLF
HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHH
HDSLCHRGFLALGSKETLDFSAYGGAFEALVKPERIYRKL
HHHHHHCCHHHCCCCCCEEEHHCCHHHHHHCCHHHHHHCC
>Mature Secondary Structure 
TSPDRTMHRDRSMDIELRLLIEAIYLQYSYDFRDYSGASVKRRVIHALRQFDCATISAL
CCCCCHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHH
QERVLHDPAAFMQLLQTLTIPVSEMFRDPSHFLAIRNEVVPVLRTYPSLKVWIAGCSTGE
HHHHHCCHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCH
EVYSTAILLREEGLLERTIIYATDINPSSLEKAKQGIFSMDNVRAYAENYRKAGGQRDFA
HHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
EYYTSAYDYAIFDKTLCENVTFADHSLATDSVFSETQFISCRNVLIYFNKKLQDRAFGLF
HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHH
HDSLCHRGFLALGSKETLDFSAYGGAFEALVKPERIYRKL
HHHHHHCCHHHCCCCCCEEEHHCCHHHHHHCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Ca2+; Mg2+ [C]

Kcat value (1/min): 10 [C]

Specific activity: NA

Km value (mM): 0.017 {S-adenosyl-L-methionine}} 0.01 {S-adenosyl-L-methionine}} [C]

Substrates: S-adenosyl-L-methionine; protein L-glutamate

Specific reaction: S-adenosyl-L-methionine + protein L-glutamate = S-adenosyl-L-homocysteine + protein L-glutamate methyl ester

General reaction: Methyl group transfer [C]

Inhibitor: A9145C; Ca2+; Methylated receptor protein; S-Adenosyl -L-homocysteine; Sinefungin [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8497190 [H]