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The map label for this gene is puuB [H]

Identifier: 66045663

GI number: 66045663

Start: 2807649

End: 2808932

Strand: Reverse

Name: puuB [H]

Synonym: Psyr_2427

Alternate gene names: 66045663

Gene position: 2808932-2807649 (Counterclockwise)

Preceding gene: 66045664

Following gene: 66045661

Centisome position: 46.1

GC content: 61.37

Gene sequence:

>1284_bases
ATGGCAAACACGCCCTACCCGCAGTCCTATTACGCGGCTTCAGCCAATGCTGCCCCGGAACGTCCCGTGCTGCAGGGCGA
AGTCGAAACTGACGTGTGTGTCATCGGAGCCGGTTACACAGGCTTGTCCAGTGCTCTGTTTCTGCTGGAAAACGGATTTC
GGGTCACGGTGCTGGAAGCGGCGAAAGTCGGCTTCGGCGCATCGGGGCGTAATGGCGGGCAGATCGTCAACAGCTACAGC
CGCGACATCGATGTCATCGAGCGAACCGTGGGGCCACGCCAGGCGCAATTGCTCGGGCAGATGGCCTTCGAAGGCGCATC
GATCATTCGGGATCGGGTTTCCCGTTACGGCATTCAGTGCGACCTGAAAGATGGCGGTGTATTCGCAGCGCTGACCAGCA
AGCAGCTGGCGCATCTCGAGGCCCAGCAGCGGCTCTGGGAGCGCTACGGGCATACCGGCCTCGAGCTGATGGACAAGCGC
CGGATCAATGAAGTTGTGGCCTGTGATCAGTACATCGGCGGGTTGCTGGACATGACCGGTGGGCATATTCATCCACTCAA
CCTGGCGCTGGGTGAAGCCGCCGCTGTCGAGACGCTGGGTGGCACCCTCTATGAGCAATCAGCGGCCATTCGTATCGAGC
GCGGGGCAAATCCTGTGGTGCATACGGCGCAAGGCAGGGTCCGGGCCAAGTTCATTATCGTGGCCGGCAATGCCTACCTG
GGCAATCTGGTCCCGGAGCTGGCGGCCAAGTCGATGCCATGTGGCACTCAGGTCATCACCACCGAGCCGTTGAGCGATGA
ACTGGCCAAGACACTTCTGCCGCAGGACTACTGCGTCGAAGACTGTAATTACCTGCTGGATTACTACCGTCTCAGCGCCG
ACAAGCGCCTGATCTTCGGCGGCGGCGTGGTGTACGGTGCCAGAGACCCCGCCAATATCGAAGCCATCATCCGGCCAAAA
ATGCTCAAGGCGTTTCCGCAGCTCAAGAACGTAAAGATCGACTACGCATGGACCGGCAACTTCCTGCTGACCCTGTCTCG
TCTGCCCCAGGTTGGACGACTGGGCGACAACATCTATTACTCGCAGGGCTGCAGCGGACACGGTGTGACCTACACCCACC
TGGCCGGCAAAGTACTGGCCGAGGCGCTGCGCGGTCAGGCAGAGCGCTTCGATGCGTTCGCCGACCTGCCCCACTACCCG
TTCCCCGGCGGACAGGTGCTGCGCACCCCGCTGACTGCGCTGGGCGCCTGGTATTACAGCCTGCGTGACCGACTCGGTTT
CTAG

Upstream 100 bases:

>100_bases
CAATCAACAGGCAACGGCTCTTTTTGCCTGCTGCTGGCAGAGCTTTTACATCTCGGGTACGATTGCCGCCATCGTCAAGA
ATATTAAACGGAGTCTCAGG

Downstream 100 bases:

>100_bases
GTTCGCTGAACGTAAAACGGCACCTTACAGGTGCCGTTTTTGTTCCTGCCGCGTTGAGTTCATTTAACGCCGCGTGCCAG
CGTTCTTTTCGCCTCGCCCG

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MANTPYPQSYYAASANAAPERPVLQGEVETDVCVIGAGYTGLSSALFLLENGFRVTVLEAAKVGFGASGRNGGQIVNSYS
RDIDVIERTVGPRQAQLLGQMAFEGASIIRDRVSRYGIQCDLKDGGVFAALTSKQLAHLEAQQRLWERYGHTGLELMDKR
RINEVVACDQYIGGLLDMTGGHIHPLNLALGEAAAVETLGGTLYEQSAAIRIERGANPVVHTAQGRVRAKFIIVAGNAYL
GNLVPELAAKSMPCGTQVITTEPLSDELAKTLLPQDYCVEDCNYLLDYYRLSADKRLIFGGGVVYGARDPANIEAIIRPK
MLKAFPQLKNVKIDYAWTGNFLLTLSRLPQVGRLGDNIYYSQGCSGHGVTYTHLAGKVLAEALRGQAERFDAFADLPHYP
FPGGQVLRTPLTALGAWYYSLRDRLGF

Sequences:

>Translated_427_residues
MANTPYPQSYYAASANAAPERPVLQGEVETDVCVIGAGYTGLSSALFLLENGFRVTVLEAAKVGFGASGRNGGQIVNSYS
RDIDVIERTVGPRQAQLLGQMAFEGASIIRDRVSRYGIQCDLKDGGVFAALTSKQLAHLEAQQRLWERYGHTGLELMDKR
RINEVVACDQYIGGLLDMTGGHIHPLNLALGEAAAVETLGGTLYEQSAAIRIERGANPVVHTAQGRVRAKFIIVAGNAYL
GNLVPELAAKSMPCGTQVITTEPLSDELAKTLLPQDYCVEDCNYLLDYYRLSADKRLIFGGGVVYGARDPANIEAIIRPK
MLKAFPQLKNVKIDYAWTGNFLLTLSRLPQVGRLGDNIYYSQGCSGHGVTYTHLAGKVLAEALRGQAERFDAFADLPHYP
FPGGQVLRTPLTALGAWYYSLRDRLGF
>Mature_426_residues
ANTPYPQSYYAASANAAPERPVLQGEVETDVCVIGAGYTGLSSALFLLENGFRVTVLEAAKVGFGASGRNGGQIVNSYSR
DIDVIERTVGPRQAQLLGQMAFEGASIIRDRVSRYGIQCDLKDGGVFAALTSKQLAHLEAQQRLWERYGHTGLELMDKRR
INEVVACDQYIGGLLDMTGGHIHPLNLALGEAAAVETLGGTLYEQSAAIRIERGANPVVHTAQGRVRAKFIIVAGNAYLG
NLVPELAAKSMPCGTQVITTEPLSDELAKTLLPQDYCVEDCNYLLDYYRLSADKRLIFGGGVVYGARDPANIEAIIRPKM
LKAFPQLKNVKIDYAWTGNFLLTLSRLPQVGRLGDNIYYSQGCSGHGVTYTHLAGKVLAEALRGQAERFDAFADLPHYPF
PGGQVLRTPLTALGAWYYSLRDRLGF

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=424, Percent_Identity=65.8018867924528, Blast_Score=577, Evalue=1e-166,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 46440; Mature: 46308

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANTPYPQSYYAASANAAPERPVLQGEVETDVCVIGAGYTGLSSALFLLENGFRVTVLEA
CCCCCCCCCCEECCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCEEEEEEE
AKVGFGASGRNGGQIVNSYSRDIDVIERTVGPRQAQLLGQMAFEGASIIRDRVSRYGIQC
ECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
DLKDGGVFAALTSKQLAHLEAQQRLWERYGHTGLELMDKRRINEVVACDQYIGGLLDMTG
EECCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
GHIHPLNLALGEAAAVETLGGTLYEQSAAIRIERGANPVVHTAQGRVRAKFIIVAGNAYL
CCEEEEHEECCCHHHHHHHCCHHHCCCCEEEEECCCCCEEEECCCCEEEEEEEEECCHHH
GNLVPELAAKSMPCGTQVITTEPLSDELAKTLLPQDYCVEDCNYLLDYYRLSADKRLIFG
HHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEE
GGVVYGARDPANIEAIIRPKMLKAFPQLKNVKIDYAWTGNFLLTLSRLPQVGRLGDNIYY
CEEEECCCCCCCCEEEECHHHHHHCHHHHCEEEEEEECCCCEEEEHHCCCCCCCCCCEEE
SQGCSGHGVTYTHLAGKVLAEALRGQAERFDAFADLPHYPFPGGQVLRTPLTALGAWYYS
ECCCCCCCEEHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
LRDRLGF
HHHHCCC
>Mature Secondary Structure 
ANTPYPQSYYAASANAAPERPVLQGEVETDVCVIGAGYTGLSSALFLLENGFRVTVLEA
CCCCCCCCCEECCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCEEEEEEE
AKVGFGASGRNGGQIVNSYSRDIDVIERTVGPRQAQLLGQMAFEGASIIRDRVSRYGIQC
ECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
DLKDGGVFAALTSKQLAHLEAQQRLWERYGHTGLELMDKRRINEVVACDQYIGGLLDMTG
EECCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
GHIHPLNLALGEAAAVETLGGTLYEQSAAIRIERGANPVVHTAQGRVRAKFIIVAGNAYL
CCEEEEHEECCCHHHHHHHCCHHHCCCCEEEEECCCCCEEEECCCCEEEEEEEEECCHHH
GNLVPELAAKSMPCGTQVITTEPLSDELAKTLLPQDYCVEDCNYLLDYYRLSADKRLIFG
HHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEE
GGVVYGARDPANIEAIIRPKMLKAFPQLKNVKIDYAWTGNFLLTLSRLPQVGRLGDNIYY
CEEEECCCCCCCCEEEECHHHHHHCHHHHCEEEEEEECCCCEEEEHHCCCCCCCCCCEEE
SQGCSGHGVTYTHLAGKVLAEALRGQAERFDAFADLPHYPFPGGQVLRTPLTALGAWYYS
ECCCCCCCEEHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
LRDRLGF
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]