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The map label for this gene is acrA [H]

Identifier: 66045523

GI number: 66045523

Start: 2653659

End: 2654819

Strand: Reverse

Name: acrA [H]

Synonym: Psyr_2283

Alternate gene names: 66045523

Gene position: 2654819-2653659 (Counterclockwise)

Preceding gene: 66045534

Following gene: 66045522

Centisome position: 43.57

GC content: 63.82

Gene sequence:

>1161_bases
ATGAGTAGTTTCAGCGATTGGCGTGTCATGCCTGCAGTAGCCCTGCTGGCAGCGTTGGGTCTGTCCGGGTGCGAGCAGAA
ACAGCAGCAGGATCACGCTGCATCAACAGCGCCCAAAGAGGTGGAAGCCATTACCGTCAACACCGAATCGTTCACAGTGG
TTGACGAATTGCCCGGCCGTATCGAACCCGTGCGTGTGGCGCAGGTTCGTGCCCGGGTGGCCGGTATCGTGCTGAGCCGC
AACTTCGAGGAAGGCGCCGACGTGAAAGCCGGCGCAGTGCTGTTCCAGATTGATCCGGCGCCGTTCAAGGCCGCGCTGTC
CAAGGCGCAGGGAGATCTGGCACGGACCCAGGCCACGTTGTTCGAGACTCAGGCTACGGTCAAGCGCTATGAGTCGCTGG
TGGAAATTGAAGCGGTGAGTCGTCAGACCTTCGACACGGCCCGCTCCGCGCTGCAAAACGCTGTCGCAGCGAAGCGTTCA
GCCGAGGCCGATGTGGAAACAGCCAGGCTGAACCTGGGCTATGCCACCGTCAAGGCGCCGATTTCGGGCCGCATCGGCCG
CGCGATGGTCACCGAGGGCGCGCTGGTCGGTCAGGGCGAAACCACCCTGCTGGCCACCATTCAACAACTCGACCCGGTCT
ACGCCGACTTCACCCAGCCGGCTGCCGAGGCCTTGCAGATGCGCGCTGCAATACAGGAAGGCCGCTTGCCCAAGGGTGGC
GAAGACGCGTTGTCGCTGAGTGTCGACGGCACCGACTACACGAGCACCGGCACCCTGCTGTTTACCGATGTTGCAGTAGA
CCGGACCACCGGTCAGGTGTCGTTGCGGGCCCGCTTTGCCAATCCCAAGGGCGTGCTGCTGCCCGGTATGTACGTGCGGG
TACGCACACCGCAACGCGTCGACAGCAACGCCATTCTTGTTCCGCAACGTGCCGTTCAGCGCTCCGGTGACGGTGCCGCC
CGCGTGCTGGTGATTGCCCAGGACGGCACGGTCGAAGCGCGTCCGGTCAAAACCGGGGCGATGCAGGATTCGCGCTGGCA
GATCACCGAAGGCCTGAAGGCAGGCGAACAGGTCATTGTCGGCAACATGACCGGGCTCAGCGCCGGTGACAAGGTCGCCC
GCAAATCGCCCTCCGCCCAGCCGCAGGCCAAGGCGCAATAA

Upstream 100 bases:

>100_bases
AATTAGTCACTAAAGCCTCATGTGAGTCAATATTGACTCATCGCCTTTCAGAGTGGATCATTCTCGGCTTTAGCATCTGA
TGGCTGCGGCCGGGTGAACG

Downstream 100 bases:

>100_bases
AGCCGCCTTCAGCGCGACCGAGGATCATTGTCCATGTCTCTCTTCTTTATCAAGCGCCCCAACTTCGCCTGGGTACTCGC
CCTGTTCATCCTGCTCGCCG

Product: secretion protein HlyD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MSSFSDWRVMPAVALLAALGLSGCEQKQQQDHAASTAPKEVEAITVNTESFTVVDELPGRIEPVRVAQVRARVAGIVLSR
NFEEGADVKAGAVLFQIDPAPFKAALSKAQGDLARTQATLFETQATVKRYESLVEIEAVSRQTFDTARSALQNAVAAKRS
AEADVETARLNLGYATVKAPISGRIGRAMVTEGALVGQGETTLLATIQQLDPVYADFTQPAAEALQMRAAIQEGRLPKGG
EDALSLSVDGTDYTSTGTLLFTDVAVDRTTGQVSLRARFANPKGVLLPGMYVRVRTPQRVDSNAILVPQRAVQRSGDGAA
RVLVIAQDGTVEARPVKTGAMQDSRWQITEGLKAGEQVIVGNMTGLSAGDKVARKSPSAQPQAKAQ

Sequences:

>Translated_386_residues
MSSFSDWRVMPAVALLAALGLSGCEQKQQQDHAASTAPKEVEAITVNTESFTVVDELPGRIEPVRVAQVRARVAGIVLSR
NFEEGADVKAGAVLFQIDPAPFKAALSKAQGDLARTQATLFETQATVKRYESLVEIEAVSRQTFDTARSALQNAVAAKRS
AEADVETARLNLGYATVKAPISGRIGRAMVTEGALVGQGETTLLATIQQLDPVYADFTQPAAEALQMRAAIQEGRLPKGG
EDALSLSVDGTDYTSTGTLLFTDVAVDRTTGQVSLRARFANPKGVLLPGMYVRVRTPQRVDSNAILVPQRAVQRSGDGAA
RVLVIAQDGTVEARPVKTGAMQDSRWQITEGLKAGEQVIVGNMTGLSAGDKVARKSPSAQPQAKAQ
>Mature_385_residues
SSFSDWRVMPAVALLAALGLSGCEQKQQQDHAASTAPKEVEAITVNTESFTVVDELPGRIEPVRVAQVRARVAGIVLSRN
FEEGADVKAGAVLFQIDPAPFKAALSKAQGDLARTQATLFETQATVKRYESLVEIEAVSRQTFDTARSALQNAVAAKRSA
EADVETARLNLGYATVKAPISGRIGRAMVTEGALVGQGETTLLATIQQLDPVYADFTQPAAEALQMRAAIQEGRLPKGGE
DALSLSVDGTDYTSTGTLLFTDVAVDRTTGQVSLRARFANPKGVLLPGMYVRVRTPQRVDSNAILVPQRAVQRSGDGAAR
VLVIAQDGTVEARPVKTGAMQDSRWQITEGLKAGEQVIVGNMTGLSAGDKVARKSPSAQPQAKAQ

Specific function: AcrAB is a drug efflux protein with a broad substrate specificity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=362, Percent_Identity=45.5801104972376, Blast_Score=297, Evalue=8e-82,
Organism=Escherichia coli, GI1789665, Length=376, Percent_Identity=43.6170212765958, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI1789929, Length=374, Percent_Identity=40.3743315508021, Blast_Score=260, Evalue=1e-70,
Organism=Escherichia coli, GI87082036, Length=319, Percent_Identity=32.6018808777429, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI87081796, Length=332, Percent_Identity=26.2048192771084, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1789637, Length=179, Percent_Identity=28.4916201117318, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 40927; Mature: 40796

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSFSDWRVMPAVALLAALGLSGCEQKQQQDHAASTAPKEVEAITVNTESFTVVDELPGR
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCEEEHHHCCCC
IEPVRVAQVRARVAGIVLSRNFEEGADVKAGAVLFQIDPAPFKAALSKAQGDLARTQATL
CCCHHHHHHHHHHHHHHEECCCCCCCCCCCCEEEEEECCCHHHHHHHHHHCHHHHHHHHH
FETQATVKRYESLVEIEAVSRQTFDTARSALQNAVAAKRSAEADVETARLNLGYATVKAP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEEECC
ISGRIGRAMVTEGALVGQGETTLLATIQQLDPVYADFTQPAAEALQMRAAIQEGRLPKGG
CCCCCCHHEEECCEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
EDALSLSVDGTDYTSTGTLLFTDVAVDRTTGQVSLRARFANPKGVLLPGMYVRVRTPQRV
CCEEEEEECCCCCCCCCCEEEEEEEEECCCCEEEEEEEECCCCCEEECCEEEEEECCCCC
DSNAILVPQRAVQRSGDGAARVLVIAQDGTVEARPVKTGAMQDSRWQITEGLKAGEQVIV
CCCEEEECHHHHHCCCCCCEEEEEEECCCCEEECCCCCCCCCCCCCHHHHHCCCCCEEEE
GNMTGLSAGDKVARKSPSAQPQAKAQ
ECCCCCCCCCHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SSFSDWRVMPAVALLAALGLSGCEQKQQQDHAASTAPKEVEAITVNTESFTVVDELPGR
CCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCEEEHHHCCCC
IEPVRVAQVRARVAGIVLSRNFEEGADVKAGAVLFQIDPAPFKAALSKAQGDLARTQATL
CCCHHHHHHHHHHHHHHEECCCCCCCCCCCCEEEEEECCCHHHHHHHHHHCHHHHHHHHH
FETQATVKRYESLVEIEAVSRQTFDTARSALQNAVAAKRSAEADVETARLNLGYATVKAP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEEECC
ISGRIGRAMVTEGALVGQGETTLLATIQQLDPVYADFTQPAAEALQMRAAIQEGRLPKGG
CCCCCCHHEEECCEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
EDALSLSVDGTDYTSTGTLLFTDVAVDRTTGQVSLRARFANPKGVLLPGMYVRVRTPQRV
CCEEEEEECCCCCCCCCCEEEEEEEEECCCCEEEEEEEECCCCCEEECCEEEEEECCCCC
DSNAILVPQRAVQRSGDGAARVLVIAQDGTVEARPVKTGAMQDSRWQITEGLKAGEQVIV
CCCEEEECHHHHHCCCCCCEEEEEEECCCCEEECCCCCCCCCCCCCHHHHHCCCCCEEEE
GNMTGLSAGDKVARKSPSAQPQAKAQ
ECCCCCCCCCHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]