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The map label for this gene is topB [H]

Identifier: 66045484

GI number: 66045484

Start: 2608140

End: 2610110

Strand: Reverse

Name: topB [H]

Synonym: Psyr_2244

Alternate gene names: 66045484

Gene position: 2610110-2608140 (Counterclockwise)

Preceding gene: 66045485

Following gene: 66045483

Centisome position: 42.83

GC content: 63.93

Gene sequence:

>1971_bases
ATGCGGCTGTTTCTCTGCGAAAAACCCTCCCAGGCCAAAGATATTGCGGCTGCGCTCGGCGCAACCCGGCGTGGCGACGG
CTGCTGGGTGGGTGCCAATGCGACGGTAACCTGGTGCATCGGCCACCTGCTGGAAACCGCCCCGCCCGATGCCTACGATG
CACGTTACAAACGCTGGGTACTGGAAGACCTGCCCATCGTGCCGGAAAAATGGAAGATGCTGGTCAAGCCCAGAACCGCC
AGCCAGTTCAAGGCAGTCAAGCGGCTGCTGGGTGAAGCGACGGAGCTGGTGATTGCCACCGACGCCGACCGTGAGGGTGA
AATGATCGCCCGCGAACTGATCGAGCACTGTCGCTATCGCGGGCCGATTCAGCGTTTGTGGCTGTCGGCACTGGATGACG
CCTCGATCCGCAAGGCACTGGCGGCGCTCAAGCCCGGTGCCGATACGTTCAGCCTGTATCACTCGGCACTGGGCCGATCA
CGTGCCGACTGGCTGATCGGCATGAACATGAGCCGCCTGTTCACCCTGCTGGGTCGGCAATCCGGTTATCAGGGCGTCCT
TCCGGTCGGCCGGGTACAAACCCCGACCCTGCGACTGGTGGTGGACCGTGATCGCAGTATCGCCAACTTTGTCCCGGCGC
CCTATTGGGCTATCGACGTCAGGCTGCTGCATGACAATCAGGCGTTCATTGCCCAGTGGCGCGCACCCTCGGATGCCTGC
GACGATCAGGACCGCTGCCTGAATCAGGCGCTGGCGCAACAGGCTGCGCAAGCGATGCGCGCCGCAGCCAGCGCACGGCT
GGTCAAGCTGCGCACCGAGCGCATTCGCGAAGCGGCGCCCTTGCCGTTCGACCTGGGCACCCTGCAGGAAATCTGCTCGA
AAAAGCTCGGGCTCGGCGCGCAGGAAACCCTGGACATCGCCCAGTCCCTGTATGAAACCCACAAGGTCATCACCTACCCG
CGCAGCGATTGCGGATACCTGCCGAACAGTCAGCACGGTGAAGCCGCAGGCATCATCGCGGCGCTGTGCAAGGCCGACCC
TGCGCTGGCAAACCTGTCGCCGCACCTCGACCCACAACGTCGGTCACGGGCCTGGAATGACAGCAAAGTGACGGCCCACC
ACGGCATCATTCCCACCGCAGCTGTACGCGGCGTAGAACGTCTGAGCGGTAAACCGCGAGCGGTGTACACCCTGATCAGG
GCGCGTTATCTGGCTCAGTTTCTGCCCAACCATGAATACGACCGCACCCAGGCCGATTTCGACTGTGCAGGCCACGCGCT
GCGTGCGGTGGGCAAGCAGATTGTCGAGCCGGGCTGGAAGCGTGCAATGCCCGAAGCGCTGGCCCCGGCCAAGGGTCGCG
AAGCGCCTGCCCCGCAGCCGTTGCCGGCATTGTTTCAGGGCTGCGAGTGTGCAGTCGCCGAGGTCATCCTCAAGGACCTC
TGGACCCAGCCGCCCAAGCCGTTCACCGAGGGAGACCTGATCAAGGCGATGAAGAACGTCGCCAAACTGGTGGAAGACCC
GCTGCTCAAACAGAAGCTCAAGGACACCACCGGCATCGGCACCGAAGCCACCCGCGCCGGGATCATTCAAGGCCTGCTGG
ACCGTGGCTACCTGACCAAGCAGGGCAAGTCGCTGTGCGCAGCTCCGGCAGCCTTCAGCCTGATCGACGCCGTGCCCCGT
GCCATCGCCGATCCGGGCACCACCGCCATCTGGGAACAGGCGCTGGACATGGTCCAGAGCGGCGAAATGAGCCTTGAAGA
GTTCGTCGCCAAACAGGCGGCGTGGATGAGCAAACAGGTGAGCCGTTGCGTCGGCATGCGTATGAGCATCAGCGGACCGG
CCAGCCCGGCAGGCGTTAAACCGCCCTGGAAGAAAAAACGCAAGACCACAACGCGCGTCGCCAAGACCGCCACAACGGAC
ACTGCGGCGAAAAAGCCGCGGCGTGCTGTCAGACCGGCCGCCAAGGCGTAG

Upstream 100 bases:

>100_bases
ACAGTGCGTTTATCTGTATATCCATACAGATAAAACTTGCCCGCTACGTATTCAACGTTCATCCTTGCAGCCCTTGCACC
GGTAATCGACGACGGTAGTC

Downstream 100 bases:

>100_bases
GCCGCAACAGCGATAGCCAGACCAGCCATTGCACATTCAAGCGTTACGAACAGGTGTTACAACATGTCAGACCGAGAGCT
CATCATCCCGCCCACCATGC

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 656; Mature: 656

Protein sequence:

>656_residues
MRLFLCEKPSQAKDIAAALGATRRGDGCWVGANATVTWCIGHLLETAPPDAYDARYKRWVLEDLPIVPEKWKMLVKPRTA
SQFKAVKRLLGEATELVIATDADREGEMIARELIEHCRYRGPIQRLWLSALDDASIRKALAALKPGADTFSLYHSALGRS
RADWLIGMNMSRLFTLLGRQSGYQGVLPVGRVQTPTLRLVVDRDRSIANFVPAPYWAIDVRLLHDNQAFIAQWRAPSDAC
DDQDRCLNQALAQQAAQAMRAAASARLVKLRTERIREAAPLPFDLGTLQEICSKKLGLGAQETLDIAQSLYETHKVITYP
RSDCGYLPNSQHGEAAGIIAALCKADPALANLSPHLDPQRRSRAWNDSKVTAHHGIIPTAAVRGVERLSGKPRAVYTLIR
ARYLAQFLPNHEYDRTQADFDCAGHALRAVGKQIVEPGWKRAMPEALAPAKGREAPAPQPLPALFQGCECAVAEVILKDL
WTQPPKPFTEGDLIKAMKNVAKLVEDPLLKQKLKDTTGIGTEATRAGIIQGLLDRGYLTKQGKSLCAAPAAFSLIDAVPR
AIADPGTTAIWEQALDMVQSGEMSLEEFVAKQAAWMSKQVSRCVGMRMSISGPASPAGVKPPWKKKRKTTTRVAKTATTD
TAAKKPRRAVRPAAKA

Sequences:

>Translated_656_residues
MRLFLCEKPSQAKDIAAALGATRRGDGCWVGANATVTWCIGHLLETAPPDAYDARYKRWVLEDLPIVPEKWKMLVKPRTA
SQFKAVKRLLGEATELVIATDADREGEMIARELIEHCRYRGPIQRLWLSALDDASIRKALAALKPGADTFSLYHSALGRS
RADWLIGMNMSRLFTLLGRQSGYQGVLPVGRVQTPTLRLVVDRDRSIANFVPAPYWAIDVRLLHDNQAFIAQWRAPSDAC
DDQDRCLNQALAQQAAQAMRAAASARLVKLRTERIREAAPLPFDLGTLQEICSKKLGLGAQETLDIAQSLYETHKVITYP
RSDCGYLPNSQHGEAAGIIAALCKADPALANLSPHLDPQRRSRAWNDSKVTAHHGIIPTAAVRGVERLSGKPRAVYTLIR
ARYLAQFLPNHEYDRTQADFDCAGHALRAVGKQIVEPGWKRAMPEALAPAKGREAPAPQPLPALFQGCECAVAEVILKDL
WTQPPKPFTEGDLIKAMKNVAKLVEDPLLKQKLKDTTGIGTEATRAGIIQGLLDRGYLTKQGKSLCAAPAAFSLIDAVPR
AIADPGTTAIWEQALDMVQSGEMSLEEFVAKQAAWMSKQVSRCVGMRMSISGPASPAGVKPPWKKKRKTTTRVAKTATTD
TAAKKPRRAVRPAAKA
>Mature_656_residues
MRLFLCEKPSQAKDIAAALGATRRGDGCWVGANATVTWCIGHLLETAPPDAYDARYKRWVLEDLPIVPEKWKMLVKPRTA
SQFKAVKRLLGEATELVIATDADREGEMIARELIEHCRYRGPIQRLWLSALDDASIRKALAALKPGADTFSLYHSALGRS
RADWLIGMNMSRLFTLLGRQSGYQGVLPVGRVQTPTLRLVVDRDRSIANFVPAPYWAIDVRLLHDNQAFIAQWRAPSDAC
DDQDRCLNQALAQQAAQAMRAAASARLVKLRTERIREAAPLPFDLGTLQEICSKKLGLGAQETLDIAQSLYETHKVITYP
RSDCGYLPNSQHGEAAGIIAALCKADPALANLSPHLDPQRRSRAWNDSKVTAHHGIIPTAAVRGVERLSGKPRAVYTLIR
ARYLAQFLPNHEYDRTQADFDCAGHALRAVGKQIVEPGWKRAMPEALAPAKGREAPAPQPLPALFQGCECAVAEVILKDL
WTQPPKPFTEGDLIKAMKNVAKLVEDPLLKQKLKDTTGIGTEATRAGIIQGLLDRGYLTKQGKSLCAAPAAFSLIDAVPR
AIADPGTTAIWEQALDMVQSGEMSLEEFVAKQAAWMSKQVSRCVGMRMSISGPASPAGVKPPWKKKRKTTTRVAKTATTD
TAAKKPRRAVRPAAKA

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=635, Percent_Identity=25.1968503937008, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI4507635, Length=506, Percent_Identity=23.7154150197628, Blast_Score=103, Evalue=4e-22,
Organism=Escherichia coli, GI1788061, Length=603, Percent_Identity=41.6252072968491, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI1787529, Length=511, Percent_Identity=27.2015655577299, Blast_Score=134, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI32563869, Length=622, Percent_Identity=24.7588424437299, Blast_Score=115, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17555378, Length=641, Percent_Identity=23.8689547581903, Blast_Score=114, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6323263, Length=623, Percent_Identity=25.0401284109149, Blast_Score=98, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24585251, Length=649, Percent_Identity=26.502311248074, Blast_Score=125, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24640096, Length=628, Percent_Identity=21.4968152866242, Blast_Score=92, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 71862; Mature: 71862

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLFLCEKPSQAKDIAAALGATRRGDGCWVGANATVTWCIGHLLETAPPDAYDARYKRWV
CEEEEECCCCHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHH
LEDLPIVPEKWKMLVKPRTASQFKAVKRLLGEATELVIATDADREGEMIARELIEHCRYR
HHCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCHHEEEEEECCCCCHHHHHHHHHHHHHCC
GPIQRLWLSALDDASIRKALAALKPGADTFSLYHSALGRSRADWLIGMNMSRLFTLLGRQ
CHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEECCCHHHHHHHHHCC
SGYQGVLPVGRVQTPTLRLVVDRDRSIANFVPAPYWAIDVRLLHDNQAFIAQWRAPSDAC
CCCCCCCCCCCCCCCEEEEEEECCCCHHHCCCCCEEEEEEEEEECCCHHHHCCCCCCCCC
DDQDRCLNQALAQQAAQAMRAAASARLVKLRTERIREAAPLPFDLGTLQEICSKKLGLGA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCH
QETLDIAQSLYETHKVITYPRSDCGYLPNSQHGEAAGIIAALCKADPALANLSPHLDPQR
HHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHCCCCCCCHHH
RSRAWNDSKVTAHHGIIPTAAVRGVERLSGKPRAVYTLIRARYLAQFLPNHEYDRTQADF
HHCCCCCCCEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCH
DCAGHALRAVGKQIVEPGWKRAMPEALAPAKGREAPAPQPLPALFQGCECAVAEVILKDL
HHHHHHHHHHHHHHHCCCHHHCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
WTQPPKPFTEGDLIKAMKNVAKLVEDPLLKQKLKDTTGIGTEATRAGIIQGLLDRGYLTK
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCC
QGKSLCAAPAAFSLIDAVPRAIADPGTTAIWEQALDMVQSGEMSLEEFVAKQAAWMSKQV
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
SRCVGMRMSISGPASPAGVKPPWKKKRKTTTRVAKTATTDTAAKKPRRAVRPAAKA
HHHHCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHCCHHHCCCCCCC
>Mature Secondary Structure
MRLFLCEKPSQAKDIAAALGATRRGDGCWVGANATVTWCIGHLLETAPPDAYDARYKRWV
CEEEEECCCCHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHH
LEDLPIVPEKWKMLVKPRTASQFKAVKRLLGEATELVIATDADREGEMIARELIEHCRYR
HHCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCHHEEEEEECCCCCHHHHHHHHHHHHHCC
GPIQRLWLSALDDASIRKALAALKPGADTFSLYHSALGRSRADWLIGMNMSRLFTLLGRQ
CHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEECCCHHHHHHHHHCC
SGYQGVLPVGRVQTPTLRLVVDRDRSIANFVPAPYWAIDVRLLHDNQAFIAQWRAPSDAC
CCCCCCCCCCCCCCCEEEEEEECCCCHHHCCCCCEEEEEEEEEECCCHHHHCCCCCCCCC
DDQDRCLNQALAQQAAQAMRAAASARLVKLRTERIREAAPLPFDLGTLQEICSKKLGLGA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCH
QETLDIAQSLYETHKVITYPRSDCGYLPNSQHGEAAGIIAALCKADPALANLSPHLDPQR
HHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHCCCCCCCHHH
RSRAWNDSKVTAHHGIIPTAAVRGVERLSGKPRAVYTLIRARYLAQFLPNHEYDRTQADF
HHCCCCCCCEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCH
DCAGHALRAVGKQIVEPGWKRAMPEALAPAKGREAPAPQPLPALFQGCECAVAEVILKDL
HHHHHHHHHHHHHHHCCCHHHCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
WTQPPKPFTEGDLIKAMKNVAKLVEDPLLKQKLKDTTGIGTEATRAGIIQGLLDRGYLTK
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCC
QGKSLCAAPAAFSLIDAVPRAIADPGTTAIWEQALDMVQSGEMSLEEFVAKQAAWMSKQV
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
SRCVGMRMSISGPASPAGVKPPWKKKRKTTTRVAKTATTDTAAKKPRRAVRPAAKA
HHHHCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]