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The map label for this gene is kgtP [H]

Identifier: 66045419

GI number: 66045419

Start: 2532242

End: 2533618

Strand: Reverse

Name: kgtP [H]

Synonym: Psyr_2179

Alternate gene names: 66045419

Gene position: 2533618-2532242 (Counterclockwise)

Preceding gene: 66045429

Following gene: 66045418

Centisome position: 41.58

GC content: 54.68

Gene sequence:

>1377_bases
ATGTCTGCATCAACCAGCGACTCAACACGCCACACTGAAAAGTCCGATCAGATGAAAAGTCCGAATCGTGTCAGAGCCAT
CATGGGTGCCTGCTCGGGCAATCTGGTGGAGTGGTACGACTTTTTCATTTACGCCTATACCGCCATCTATTTTGCGAGCT
CGTTTTTCCCCAAAGGCGACACCACCAGTCAGTTGCTCGCAACCGCCGGCGTCTTCGCCGTCGGCTTTTTCATGCGGCCG
CTCGGCGGTTGGATTTTTGGCTGGATTGCTGATACCCGCGGCCGCAAGGTATCGATGATTATCTCGGTGTTCATGATGTG
CGCCGGTTCCTTGTTGATTGCCGTCATGCCGACCTATGAAACCATCGGTGTGGCGGCGCCAGTTTTGCTTGTCGTTGCCA
GGCTGATTCAGGGTCTGTCAGTCGGCGCGGAGTACGGCACCGGCGCCACCTACATCAGTGAAATCGCGACACCTGGGCGT
CGTTGTTTCTATGGATCATTCCAGTACTTCACCATCATCGCAGGCCAACTCCTCGCCCTGATGACCGTGGTCATACTTCA
ACAAACCCTTACCGGCGAGGAATTGCGGGAATGGGGCTGGAGAATTCCTTTTTTCATCGGCGCCTTTAGTTCTATCGTAG
TCGTGTATTTGCGTCGCGCGATGCATGAAACCGCCACAAAAAAAGAAATGAACCGCAAAGATGCCGGCTCGCTACGTGGC
ATGTTCAAACACAAACGCGCAGTGGCACTGGTGGTCGCGTTTACCATTGGCGGTTCACTTTACTTCTACACCTTCACCAC
CTACATGCAGAAATTTCTGGTGATCTCTGCGGGGTTTAGTCCGGAAACGGTGAGCTTCATCATGACGGCCGCCCTGGTCG
GTTTCATGTTTTGTCAGCCACTCTTTGGTCTGCTCGCCGACCGAATCGGAATCAAGGTCCATATGTTGTTGTTTTCCGGG
TTGGCCATGCTACTGGTGATCCCCCTGCTCTATTCACTGCAATCAGTCAGCAGTCCATTTACAGCGTTCCTGCTGGTATT
TGGCGGCCTGGCAATCGCGTCGCTCTACACCCCGATTGCCGGTATCGTCAAAGCTGAACTGTTCCCGCCAGCGGTACGTG
CATTGGGTGTGGGCTTTCCTTATGCCGTCGGCAACGCGGCATTCGGTGGCACCGCCGAATACGTCGCCTTGTCGCTTCGG
TCGCAGGGTATTGAGGAGTATTTCTTCTTCTACGTCGCGGCCGTAGTAACCATCACGTTCATCGCAGCGACGCTAATGCC
CAACCTGTCCCGCTATGGTTACATCAGCGGCTCGGGTGAGATCGAAGAACGCACCGGACTGAGGGGCTGCATCAAATCGC
AGGCTGCCAGGATCTGA

Upstream 100 bases:

>100_bases
CAGCACTTGCGTTAAGCACATCAACCCATCGAGCTAGCAACGTCGGACACGCATCAGCGTGGGCCTCGTATCGTCTCGCC
TTGAAACAAGGGTGAACAAA

Downstream 100 bases:

>100_bases
GGCACTGACGATTACCGTGGTTTTTTGAAAGGGCCCATGATGATCCATGGGCCCTTTTAAAATCACTGGATAGCGGATAC
CGATGCTACTTGTGATGAAT

Product: major facilitator transporter

Products: Proton [Cytoplasm]; 2-oxoglutarate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MSASTSDSTRHTEKSDQMKSPNRVRAIMGACSGNLVEWYDFFIYAYTAIYFASSFFPKGDTTSQLLATAGVFAVGFFMRP
LGGWIFGWIADTRGRKVSMIISVFMMCAGSLLIAVMPTYETIGVAAPVLLVVARLIQGLSVGAEYGTGATYISEIATPGR
RCFYGSFQYFTIIAGQLLALMTVVILQQTLTGEELREWGWRIPFFIGAFSSIVVVYLRRAMHETATKKEMNRKDAGSLRG
MFKHKRAVALVVAFTIGGSLYFYTFTTYMQKFLVISAGFSPETVSFIMTAALVGFMFCQPLFGLLADRIGIKVHMLLFSG
LAMLLVIPLLYSLQSVSSPFTAFLLVFGGLAIASLYTPIAGIVKAELFPPAVRALGVGFPYAVGNAAFGGTAEYVALSLR
SQGIEEYFFFYVAAVVTITFIAATLMPNLSRYGYISGSGEIEERTGLRGCIKSQAARI

Sequences:

>Translated_458_residues
MSASTSDSTRHTEKSDQMKSPNRVRAIMGACSGNLVEWYDFFIYAYTAIYFASSFFPKGDTTSQLLATAGVFAVGFFMRP
LGGWIFGWIADTRGRKVSMIISVFMMCAGSLLIAVMPTYETIGVAAPVLLVVARLIQGLSVGAEYGTGATYISEIATPGR
RCFYGSFQYFTIIAGQLLALMTVVILQQTLTGEELREWGWRIPFFIGAFSSIVVVYLRRAMHETATKKEMNRKDAGSLRG
MFKHKRAVALVVAFTIGGSLYFYTFTTYMQKFLVISAGFSPETVSFIMTAALVGFMFCQPLFGLLADRIGIKVHMLLFSG
LAMLLVIPLLYSLQSVSSPFTAFLLVFGGLAIASLYTPIAGIVKAELFPPAVRALGVGFPYAVGNAAFGGTAEYVALSLR
SQGIEEYFFFYVAAVVTITFIAATLMPNLSRYGYISGSGEIEERTGLRGCIKSQAARI
>Mature_457_residues
SASTSDSTRHTEKSDQMKSPNRVRAIMGACSGNLVEWYDFFIYAYTAIYFASSFFPKGDTTSQLLATAGVFAVGFFMRPL
GGWIFGWIADTRGRKVSMIISVFMMCAGSLLIAVMPTYETIGVAAPVLLVVARLIQGLSVGAEYGTGATYISEIATPGRR
CFYGSFQYFTIIAGQLLALMTVVILQQTLTGEELREWGWRIPFFIGAFSSIVVVYLRRAMHETATKKEMNRKDAGSLRGM
FKHKRAVALVVAFTIGGSLYFYTFTTYMQKFLVISAGFSPETVSFIMTAALVGFMFCQPLFGLLADRIGIKVHMLLFSGL
AMLLVIPLLYSLQSVSSPFTAFLLVFGGLAIASLYTPIAGIVKAELFPPAVRALGVGFPYAVGNAAFGGTAEYVALSLRS
QGIEEYFFFYVAAVVTITFIAATLMPNLSRYGYISGSGEIEERTGLRGCIKSQAARI

Specific function: Uptake of alpha-ketoglutarate across the boundary membrane with the concomitant import of a cation (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788942, Length=426, Percent_Identity=56.3380281690141, Blast_Score=451, Evalue=1e-128,
Organism=Escherichia coli, GI1790550, Length=440, Percent_Identity=31.5909090909091, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI1788292, Length=443, Percent_Identity=30.2483069977427, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1789941, Length=433, Percent_Identity=31.6397228637413, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI87082404, Length=394, Percent_Identity=25.8883248730964, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI87082231, Length=472, Percent_Identity=24.364406779661, Blast_Score=83, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 49941; Mature: 49810

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHCCHHHCEEECCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SASTSDSTRHTEKSDQMKSPNRVRAIMGACSGNLVEWYDFFIYAYTAIYFASSFFPKGD
CCCCCCCCCCCCHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCC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HHHHHHCCHHHCEEECCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 2-oxoglutarate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 2-oxoglutarate [Periplasm] = Proton [Cytoplasm] + 2-oxoglutarate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2118499; 9205837; 9278503; 1709493; 2190187; 2053984; 8419306 [H]