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The map label for this gene is kefB [H]

Identifier: 66045379

GI number: 66045379

Start: 2497985

End: 2499793

Strand: Reverse

Name: kefB [H]

Synonym: Psyr_2139

Alternate gene names: 66045379

Gene position: 2499793-2497985 (Counterclockwise)

Preceding gene: 66045381

Following gene: 66045375

Centisome position: 41.02

GC content: 60.75

Gene sequence:

>1809_bases
ATGCCCCATGAAGGCAGCCTGCTGCAGGTCGCAGTGGTATTCCTGCTCGCCGCCGTGCTGACTGTGCCGCTGGCCAAGCG
GTTGAAGCTGGGCGCGGTGATCGGCTATTTGTTCGCCGGCGTGGTCATCGGCCCTTCGGTATTGGGCCTGATCGGTGATA
CCGAGAGCGTCAGCCATATTTCCGAACTTGGCGTGGTACTGCTGCTGTTCATCATTGGCCTGGAGCTTTCGCCCAAACGT
TTATGGGTCATGCGCAAATCCGTGTTTGGCGTAGGCATGGCGCAGGTACTGCTGACCGGCCTGATCATCGGCGGGGTCGC
CTTCGCTGCCTTCGGCCAGTCGCTGAACACCGCGGCCATTCTGGGCCTGGGCCTTGCCCTGTCGTCCACGGCCTTCGGCC
TGCAGAGCCTGGCGGAACGCAAACAGCTGAACAGCCCGCACGGCAGGATGGCGTTCGCGATTCTGCTGTTTCAGGACATT
GCGGCGATCCCGCTGATTGCCATGGTGCCGTTTCTGGCCGGGGCTGACCACGCGATGGACACCGGCGAAAGCATCAACCA
CGGTCTGCGCGTGCTGGGCAGCATTGCCATTGTGGTGATTGGCGGGCGCTATTTGCTGCGCCCGGTGTTCCGGATCGTCG
CCAAGACCAAAATCCAGGAAGTTTCGACCGCCACGGCCTTGCTGGTGGTGATAGGCACAGCGTGGCTGATGGAACTGGTG
GGAGTGTCCATGGCGCTGGGCGCGTTTCTTGCTGGCCTGTTGCTGGCCGACTCCGAATACCGTCACGAACTGGAAGCACA
GATCGAACCGTTCAAAGGGTTATTGCTGGGGCTGTTCTTCATCAGTGTCGGCATGGGCGCCAACGTTGGCCTGTTGCTCA
GCTCGCCGCTGATCGTGCTGGGCCTGACGCTGTTGCTGATCGCAATCAAGCTGCCGATGCTGTTCCTGATCGGTCGCACC
CTTGGCGAACTCAACAACCGCAGCGCGCTGCAGCTGGGTCTGGTACTGGCCGCAGGCGGCGAGTTTGCCTTTGTGGTGTT
CAAGATCGGCAGCGCTCAGGGGCTGTTCGAGGCCGGGCTATACGACATGCTGGTGTTGACCATCACCTTGTCGATGGCGG
TCACGCCGTTGCTGTTCATTGCTCTGGCGCGCTGGATCAAACCCAAGGTCAAGCCTGTCGAGGTGCCGGAGGAATACCGC
GATATCCAGACCGACAAACCACGCGTCGTCATTGCCGGCATGGGCCGTATGGGACAGATTGTCGCGCGTATCCTGCGCGC
GCAGAAGATTCCTTTCATCGCCCTGGATACCGCCGTCGAGTCCATCGAGTTCTCCCGTAGCTTTGGCAATGTACCGGTCT
TCTATGGCGACCCGTTGCGCCCGGAGATACTGCGTGCCGCCAAAGTCGATGAGGCCGAATACTTCGTCATCGCGACCGAT
GATCCTGACACCAACATCAAGACTGCCGAGCTGATCCGCAAGCTGTATCCGCACATGAAGATCATTGCCCGCGCACGTAA
CCGCCAGCATGTTCATCGGTTGATGGACCTGGGCGCTCACGCGGTTCGCGAAACGTTCTATTCAAGCCTGGAAATGAGTC
GCCAGACCCTCATGGGCCTGGGCCTCAGCGAAACACAGGCCGACGCACGCATCAGTCGCTTCAAACGTCACGACGAACAG
GTGCTGGCCGCTCAGCATGAGGTGTATGACGACGACGCCAAGGTCCTGCAGACCGCCCGCGAAGCCAGGGCCGACCTCGC
GCAACTGTTCGAGGCAGACCGTCTGGACGAAGAAGCCGCGAAAAGCTGA

Upstream 100 bases:

>100_bases
ATTATCGTCATGCTGCGATGCGCGTTGCCGTGTAAAACTCCCATGCTTTAATACCGCCCCTGCTTCAACAGTGTGTTCAA
TTATTACCAAGGAGTTTTAC

Downstream 100 bases:

>100_bases
CCTGCTCATGCGCCGCAGGCTGGTCTGCGGCGCAACTCAGGCGTAGGGGCTGTCCCAGTAACGCGCGTACATGCCGAGCT
TCTTCTTCACGCCGCGGGGC

Product: potassium efflux system protein

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter; NEM-activatable K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 602; Mature: 601

Protein sequence:

>602_residues
MPHEGSLLQVAVVFLLAAVLTVPLAKRLKLGAVIGYLFAGVVIGPSVLGLIGDTESVSHISELGVVLLLFIIGLELSPKR
LWVMRKSVFGVGMAQVLLTGLIIGGVAFAAFGQSLNTAAILGLGLALSSTAFGLQSLAERKQLNSPHGRMAFAILLFQDI
AAIPLIAMVPFLAGADHAMDTGESINHGLRVLGSIAIVVIGGRYLLRPVFRIVAKTKIQEVSTATALLVVIGTAWLMELV
GVSMALGAFLAGLLLADSEYRHELEAQIEPFKGLLLGLFFISVGMGANVGLLLSSPLIVLGLTLLLIAIKLPMLFLIGRT
LGELNNRSALQLGLVLAAGGEFAFVVFKIGSAQGLFEAGLYDMLVLTITLSMAVTPLLFIALARWIKPKVKPVEVPEEYR
DIQTDKPRVVIAGMGRMGQIVARILRAQKIPFIALDTAVESIEFSRSFGNVPVFYGDPLRPEILRAAKVDEAEYFVIATD
DPDTNIKTAELIRKLYPHMKIIARARNRQHVHRLMDLGAHAVRETFYSSLEMSRQTLMGLGLSETQADARISRFKRHDEQ
VLAAQHEVYDDDAKVLQTAREARADLAQLFEADRLDEEAAKS

Sequences:

>Translated_602_residues
MPHEGSLLQVAVVFLLAAVLTVPLAKRLKLGAVIGYLFAGVVIGPSVLGLIGDTESVSHISELGVVLLLFIIGLELSPKR
LWVMRKSVFGVGMAQVLLTGLIIGGVAFAAFGQSLNTAAILGLGLALSSTAFGLQSLAERKQLNSPHGRMAFAILLFQDI
AAIPLIAMVPFLAGADHAMDTGESINHGLRVLGSIAIVVIGGRYLLRPVFRIVAKTKIQEVSTATALLVVIGTAWLMELV
GVSMALGAFLAGLLLADSEYRHELEAQIEPFKGLLLGLFFISVGMGANVGLLLSSPLIVLGLTLLLIAIKLPMLFLIGRT
LGELNNRSALQLGLVLAAGGEFAFVVFKIGSAQGLFEAGLYDMLVLTITLSMAVTPLLFIALARWIKPKVKPVEVPEEYR
DIQTDKPRVVIAGMGRMGQIVARILRAQKIPFIALDTAVESIEFSRSFGNVPVFYGDPLRPEILRAAKVDEAEYFVIATD
DPDTNIKTAELIRKLYPHMKIIARARNRQHVHRLMDLGAHAVRETFYSSLEMSRQTLMGLGLSETQADARISRFKRHDEQ
VLAAQHEVYDDDAKVLQTAREARADLAQLFEADRLDEEAAKS
>Mature_601_residues
PHEGSLLQVAVVFLLAAVLTVPLAKRLKLGAVIGYLFAGVVIGPSVLGLIGDTESVSHISELGVVLLLFIIGLELSPKRL
WVMRKSVFGVGMAQVLLTGLIIGGVAFAAFGQSLNTAAILGLGLALSSTAFGLQSLAERKQLNSPHGRMAFAILLFQDIA
AIPLIAMVPFLAGADHAMDTGESINHGLRVLGSIAIVVIGGRYLLRPVFRIVAKTKIQEVSTATALLVVIGTAWLMELVG
VSMALGAFLAGLLLADSEYRHELEAQIEPFKGLLLGLFFISVGMGANVGLLLSSPLIVLGLTLLLIAIKLPMLFLIGRTL
GELNNRSALQLGLVLAAGGEFAFVVFKIGSAQGLFEAGLYDMLVLTITLSMAVTPLLFIALARWIKPKVKPVEVPEEYRD
IQTDKPRVVIAGMGRMGQIVARILRAQKIPFIALDTAVESIEFSRSFGNVPVFYGDPLRPEILRAAKVDEAEYFVIATDD
PDTNIKTAELIRKLYPHMKIIARARNRQHVHRLMDLGAHAVRETFYSSLEMSRQTLMGLGLSETQADARISRFKRHDEQV
LAAQHEVYDDDAKVLQTAREARADLAQLFEADRLDEEAAKS

Specific function: Transport system that facilitates potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=392, Percent_Identity=26.2755102040816, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1789749, Length=597, Percent_Identity=44.2211055276382, Blast_Score=451, Evalue=1e-128,
Organism=Escherichia coli, GI1786232, Length=590, Percent_Identity=43.728813559322, Blast_Score=449, Evalue=1e-127,
Organism=Escherichia coli, GI1786685, Length=554, Percent_Identity=30.5054151624549, Blast_Score=190, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6322367, Length=427, Percent_Identity=22.4824355971897, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR020884
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 65118; Mature: 64987

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHEGSLLQVAVVFLLAAVLTVPLAKRLKLGAVIGYLFAGVVIGPSVLGLIGDTESVSHI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
SELGVVLLLFIIGLELSPKRLWVMRKSVFGVGMAQVLLTGLIIGGVAFAAFGQSLNTAAI
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
LGLGLALSSTAFGLQSLAERKQLNSPHGRMAFAILLFQDIAAIPLIAMVPFLAGADHAMD
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCC
TGESINHGLRVLGSIAIVVIGGRYLLRPVFRIVAKTKIQEVSTATALLVVIGTAWLMELV
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVSMALGAFLAGLLLADSEYRHELEAQIEPFKGLLLGLFFISVGMGANVGLLLSSPLIVL
HHHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHH
GLTLLLIAIKLPMLFLIGRTLGELNNRSALQLGLVLAAGGEFAFVVFKIGSAQGLFEAGL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHEECCCCEEEEEEEECCCCCHHHHHH
YDMLVLTITLSMAVTPLLFIALARWIKPKVKPVEVPEEYRDIQTDKPRVVIAGMGRMGQI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCEEEEECCCHHHHH
VARILRAQKIPFIALDTAVESIEFSRSFGNVPVFYGDPLRPEILRAAKVDEAEYFVIATD
HHHHHHHHCCCEEEHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHCCCCCCEEEEEEC
DPDTNIKTAELIRKLYPHMKIIARARNRQHVHRLMDLGAHAVRETFYSSLEMSRQTLMGL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GLSETQADARISRFKRHDEQVLAAQHEVYDDDAKVLQTAREARADLAQLFEADRLDEEAA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHC
KS
CC
>Mature Secondary Structure 
PHEGSLLQVAVVFLLAAVLTVPLAKRLKLGAVIGYLFAGVVIGPSVLGLIGDTESVSHI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
SELGVVLLLFIIGLELSPKRLWVMRKSVFGVGMAQVLLTGLIIGGVAFAAFGQSLNTAAI
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
LGLGLALSSTAFGLQSLAERKQLNSPHGRMAFAILLFQDIAAIPLIAMVPFLAGADHAMD
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCC
TGESINHGLRVLGSIAIVVIGGRYLLRPVFRIVAKTKIQEVSTATALLVVIGTAWLMELV
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVSMALGAFLAGLLLADSEYRHELEAQIEPFKGLLLGLFFISVGMGANVGLLLSSPLIVL
HHHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHH
GLTLLLIAIKLPMLFLIGRTLGELNNRSALQLGLVLAAGGEFAFVVFKIGSAQGLFEAGL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHEECCCCEEEEEEEECCCCCHHHHHH
YDMLVLTITLSMAVTPLLFIALARWIKPKVKPVEVPEEYRDIQTDKPRVVIAGMGRMGQI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCEEEEECCCHHHHH
VARILRAQKIPFIALDTAVESIEFSRSFGNVPVFYGDPLRPEILRAAKVDEAEYFVIATD
HHHHHHHHCCCEEEHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHCCCCCCEEEEEEC
DPDTNIKTAELIRKLYPHMKIIARARNRQHVHRLMDLGAHAVRETFYSSLEMSRQTLMGL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GLSETQADARISRFKRHDEQVLAAQHEVYDDDAKVLQTAREARADLAQLFEADRLDEEAA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHC
KS
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA