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The map label for this gene is cobA [H]

Identifier: 66045338

GI number: 66045338

Start: 2445837

End: 2446598

Strand: Reverse

Name: cobA [H]

Synonym: Psyr_2098

Alternate gene names: 66045338

Gene position: 2446598-2445837 (Counterclockwise)

Preceding gene: 66045339

Following gene: 66045329

Centisome position: 40.15

GC content: 62.47

Gene sequence:

>762_bases
ATGAGCGCAAAAGTATGGCTGGTAGGCGCGGGCCCCGGCGACCCGGAATTGCTGACCCTCAAGGCAGTACGCGCCTTGCA
TGAAGCCGACGTGGTGCTGATCGACGATCTGGTCAACCCCGCAGTGCTGGAGCATTGTCCGGGGGCGCGGGTGATCGCGG
TCGGCAAGCGCGGCGGTTGCCGCTCGACGCCGCAAGCGTTTATCCATCGCCTGATGCTGCGCTACGTCCGTCAGGGCAAA
TGTGTGGTCCGCCTCAAAGGCGGCGATCCGTGCATCTTCGGACGTGGTGGTGAAGAGGCTCAATGGCTGCAGGAACACGG
TGTGGCCGTGGAGCTGGTCAACGGCATTACAGCGGGTCTTGCTGGTGCAACACAGTGCGGCATTTCGCTGACCCTGCGCG
GTATAAGCCGTGGCGTGACACTGGTCACCGCCCACACTCAGGATGACAGCACGCTCAACTGGCAAGCGCTCGCGCAAGGC
GGAACCACCCTGGTGGTTTACATGGGCGTGGCGAAACTGGCGGAAATTCGCGAGAGCCTGCTGGCAGGCGGAATGAGTGG
CGAGATGCCGGTGGCGATGATCGAGAACGCTTCGCTGCCATGGCAGCGCGAATGCCGTAGCACCCTGAATGCCATGCAAC
AGGACGCAGCCGCTTTCCAGCTGAAGAGCCCCGCCATTCTGGTGATCGGCGCGGTTGCGGCTTACAGCAATGAGCTGCTG
AATCAAACCCTGATGGACCACGCGCAGCTGAATGCCAGCTGA

Upstream 100 bases:

>100_bases
CTCAAGACACAGTTGGGCTGCGGCACCCAGTGCGGCTCCTGCGTGCCGGAAATAAGAAGACTGATCCATACCGTGGCGGT
TACAGAATGAGGAATGCGGC

Downstream 100 bases:

>100_bases
CACCAGGCCCGTCTGAACAAGCACCCTGCCAAATCGCAGGCAAAGAAAAGCCCGGCCTGAGCCGGGCTTTTCAGTACAAG
CGGCTAATTACTTAGCTTGA

Product: uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM [H]

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MSAKVWLVGAGPGDPELLTLKAVRALHEADVVLIDDLVNPAVLEHCPGARVIAVGKRGGCRSTPQAFIHRLMLRYVRQGK
CVVRLKGGDPCIFGRGGEEAQWLQEHGVAVELVNGITAGLAGATQCGISLTLRGISRGVTLVTAHTQDDSTLNWQALAQG
GTTLVVYMGVAKLAEIRESLLAGGMSGEMPVAMIENASLPWQRECRSTLNAMQQDAAAFQLKSPAILVIGAVAAYSNELL
NQTLMDHAQLNAS

Sequences:

>Translated_253_residues
MSAKVWLVGAGPGDPELLTLKAVRALHEADVVLIDDLVNPAVLEHCPGARVIAVGKRGGCRSTPQAFIHRLMLRYVRQGK
CVVRLKGGDPCIFGRGGEEAQWLQEHGVAVELVNGITAGLAGATQCGISLTLRGISRGVTLVTAHTQDDSTLNWQALAQG
GTTLVVYMGVAKLAEIRESLLAGGMSGEMPVAMIENASLPWQRECRSTLNAMQQDAAAFQLKSPAILVIGAVAAYSNELL
NQTLMDHAQLNAS
>Mature_252_residues
SAKVWLVGAGPGDPELLTLKAVRALHEADVVLIDDLVNPAVLEHCPGARVIAVGKRGGCRSTPQAFIHRLMLRYVRQGKC
VVRLKGGDPCIFGRGGEEAQWLQEHGVAVELVNGITAGLAGATQCGISLTLRGISRGVTLVTAHTQDDSTLNWQALAQGG
TTLVVYMGVAKLAEIRESLLAGGMSGEMPVAMIENASLPWQRECRSTLNAMQQDAAAFQLKSPAILVIGAVAAYSNELLN
QTLMDHAQLNAS

Specific function: Catalyzes both methylations at C-2 and C-7 of uroporphyrinogen III leading to precorrin-1 and precorrin-2; their oxidative esterification gives respectively factor I octamethyl ester and sirohydrochlorin [H]

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=237, Percent_Identity=43.0379746835443, Blast_Score=177, Evalue=6e-46,
Organism=Saccharomyces cerevisiae, GI6322922, Length=248, Percent_Identity=30.6451612903226, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.107 [H]

Molecular weight: Translated: 26771; Mature: 26640

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAKVWLVGAGPGDPELLTLKAVRALHEADVVLIDDLVNPAVLEHCPGARVIAVGKRGGC
CCCEEEEEECCCCCCCEEHHHHHHHHHHCCEEEEHHHCCHHHHHHCCCCEEEEEECCCCC
RSTPQAFIHRLMLRYVRQGKCVVRLKGGDPCIFGRGGEEAQWLQEHGVAVELVNGITAGL
CCCHHHHHHHHHHHHHHCCCEEEEEECCCEEEECCCCCHHHHHHHCCCEEEEHHHHHHHC
AGATQCGISLTLRGISRGVTLVTAHTQDDSTLNWQALAQGGTTLVVYMGVAKLAEIRESL
CCHHHCCCEEEEEECCCCEEEEEEECCCCCCCCHHHHHCCCCEEEEEHHHHHHHHHHHHH
LAGGMSGEMPVAMIENASLPWQRECRSTLNAMQQDAAAFQLKSPAILVIGAVAAYSNELL
HHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHEEECCCCEEEEEHHHHHHHHHH
NQTLMDHAQLNAS
HHHHHHHHHCCCC
>Mature Secondary Structure 
SAKVWLVGAGPGDPELLTLKAVRALHEADVVLIDDLVNPAVLEHCPGARVIAVGKRGGC
CCEEEEEECCCCCCCEEHHHHHHHHHHCCEEEEHHHCCHHHHHHCCCCEEEEEECCCCC
RSTPQAFIHRLMLRYVRQGKCVVRLKGGDPCIFGRGGEEAQWLQEHGVAVELVNGITAGL
CCCHHHHHHHHHHHHHHCCCEEEEEECCCEEEECCCCCHHHHHHHCCCEEEEHHHHHHHC
AGATQCGISLTLRGISRGVTLVTAHTQDDSTLNWQALAQGGTTLVVYMGVAKLAEIRESL
CCHHHCCCEEEEEECCCCEEEEEEECCCCCCCCHHHHHCCCCEEEEEHHHHHHHHHHHHH
LAGGMSGEMPVAMIENASLPWQRECRSTLNAMQQDAAAFQLKSPAILVIGAVAAYSNELL
HHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHEEECCCCEEEEEHHHHHHHHHH
NQTLMDHAQLNAS
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7959054 [H]