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The map label for this gene is dusC [H]

Identifier: 66045219

GI number: 66045219

Start: 2297844

End: 2298809

Strand: Direct

Name: dusC [H]

Synonym: Psyr_1979

Alternate gene names: 66045219

Gene position: 2297844-2298809 (Clockwise)

Preceding gene: 66045218

Following gene: 66045220

Centisome position: 37.71

GC content: 62.84

Gene sequence:

>966_bases
ATGCAAATTGCTCTGGCGCCCATGGAGGGGTTGGTCGACGACATCCTGCGTAATGTGCTGACGAAAGTCGGCGGCATCGA
CTGGTGCGTGACCGAATTCATCCGGGTGTCCGAGCGTTTGATGCCGGCGCACTACTTCTACAAGTACGCTTCGGAGTTTC
ATAACGGCGCGAAGACTGACGCCGGTACGCCTTTACGGCTCCAGCTGTTGGGCTCCGATCCGGTGTGTCTGGCCGAGAAT
GCCGCGTTCGCCTGCGAGCTGGGCGCGCCGGTGCTGGACCTGAACTTCGGTTGCCCGGCCAAGACCGTCAACCGTTCCCG
TGGTGGCGCAGTTCTGCTCAAGGAGCCGGAACTGCTGCACACCATTGTCAGCCAGGTGCGCCGTGCTGTGCCCAAAGACA
TACCGGTCACCGCCAAGATGCGTTTGGGCTACGAAAACACCGACCTGGCGCTGGATTGCGCCAGAGCGTTGGCTGACGGC
GGGGCCGAGCAAATCGTCGTGCATGCGCGCACCAAGGTCGACGGCTACAAGCCGCCGGCGCACTGGGAGTGGATCGCGCG
CATCCAGGAAGTGGTCAAGGTCCCGGTAGTTGCCAACGGGGAAATCTGGACCGTTGAGGATTGGCGGCGATGCCGCGAAA
TCTGCGGCGCGCGGGACATCATGATCGGTCGCGGGCTGGTGGCGCGCCCTGATCTCGCCCGGCAGATTGCTGCGGCGCAG
AAGGGCGAAGAGGTGGTGCCGATGACTTGGGACGCGTTGCAGCCGATGCTGCGGGTCTTCTGGCAGGACTGTCTGGTGAA
GATGACGCTGATTCAGGCACCGGGTCGGCTCAAGCAGTGGCTGGTGCTGCTGACCAAAAGCTACCCCGAGGCGACCCTGA
TGTTCAATACGTTGCGCCGCGAGACCGACTGCGATCGCATCAGCGTATTGCTGGGCTGCGCGCCCGGTTGCTTACCCAAA
AGCTGA

Upstream 100 bases:

>100_bases
CCGAGTTCATCGAGGGCTATGGCGCAGCCATCTCTGACAGCGCCGTTCCCAATCCGCACGTCGGGCAGTAAACTGTCGGA
CTTTTTTTCGAGTGTGACCC

Downstream 100 bases:

>100_bases
TCCCGACGAACGGTCAAAAAAATCCTGAAAAAAGTCTCTTGAAAAGCGAAAGGCTGTCCCTATTTTGGTGATCAGGCGAT
GCCGAAAACGGGTCGCTTAA

Product: dihydrouridine synthase, DuS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MQIALAPMEGLVDDILRNVLTKVGGIDWCVTEFIRVSERLMPAHYFYKYASEFHNGAKTDAGTPLRLQLLGSDPVCLAEN
AAFACELGAPVLDLNFGCPAKTVNRSRGGAVLLKEPELLHTIVSQVRRAVPKDIPVTAKMRLGYENTDLALDCARALADG
GAEQIVVHARTKVDGYKPPAHWEWIARIQEVVKVPVVANGEIWTVEDWRRCREICGARDIMIGRGLVARPDLARQIAAAQ
KGEEVVPMTWDALQPMLRVFWQDCLVKMTLIQAPGRLKQWLVLLTKSYPEATLMFNTLRRETDCDRISVLLGCAPGCLPK
S

Sequences:

>Translated_321_residues
MQIALAPMEGLVDDILRNVLTKVGGIDWCVTEFIRVSERLMPAHYFYKYASEFHNGAKTDAGTPLRLQLLGSDPVCLAEN
AAFACELGAPVLDLNFGCPAKTVNRSRGGAVLLKEPELLHTIVSQVRRAVPKDIPVTAKMRLGYENTDLALDCARALADG
GAEQIVVHARTKVDGYKPPAHWEWIARIQEVVKVPVVANGEIWTVEDWRRCREICGARDIMIGRGLVARPDLARQIAAAQ
KGEEVVPMTWDALQPMLRVFWQDCLVKMTLIQAPGRLKQWLVLLTKSYPEATLMFNTLRRETDCDRISVLLGCAPGCLPK
S
>Mature_321_residues
MQIALAPMEGLVDDILRNVLTKVGGIDWCVTEFIRVSERLMPAHYFYKYASEFHNGAKTDAGTPLRLQLLGSDPVCLAEN
AAFACELGAPVLDLNFGCPAKTVNRSRGGAVLLKEPELLHTIVSQVRRAVPKDIPVTAKMRLGYENTDLALDCARALADG
GAEQIVVHARTKVDGYKPPAHWEWIARIQEVVKVPVVANGEIWTVEDWRRCREICGARDIMIGRGLVARPDLARQIAAAQ
KGEEVVPMTWDALQPMLRVFWQDCLVKMTLIQAPGRLKQWLVLLTKSYPEATLMFNTLRRETDCDRISVLLGCAPGCLPK
S

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusC subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=229, Percent_Identity=28.82096069869, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI40807366, Length=173, Percent_Identity=31.7919075144509, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI239788483, Length=173, Percent_Identity=29.4797687861272, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI239788462, Length=148, Percent_Identity=31.0810810810811, Blast_Score=72, Evalue=8e-13,
Organism=Escherichia coli, GI1788462, Length=300, Percent_Identity=41.3333333333333, Blast_Score=233, Evalue=1e-62,
Organism=Escherichia coli, GI1789660, Length=250, Percent_Identity=30.8, Blast_Score=110, Evalue=9e-26,
Organism=Escherichia coli, GI145693211, Length=245, Percent_Identity=25.7142857142857, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI25144369, Length=225, Percent_Identity=31.1111111111111, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17507177, Length=200, Percent_Identity=31.5, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17543114, Length=170, Percent_Identity=27.6470588235294, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323560, Length=203, Percent_Identity=29.064039408867, Blast_Score=83, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6323437, Length=205, Percent_Identity=29.2682926829268, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI19921524, Length=188, Percent_Identity=31.9148936170213, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24580595, Length=229, Percent_Identity=27.9475982532751, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19920448, Length=229, Percent_Identity=27.9475982532751, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24585320, Length=233, Percent_Identity=26.6094420600858, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI45549423, Length=141, Percent_Identity=32.6241134751773, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 35627; Mature: 35627

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIALAPMEGLVDDILRNVLTKVGGIDWCVTEFIRVSERLMPAHYFYKYASEFHNGAKTD
CEEEECHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
AGTPLRLQLLGSDPVCLAENAAFACELGAPVLDLNFGCPAKTVNRSRGGAVLLKEPELLH
CCCCEEEEEECCCCEEEECCCCEEEECCCCEEECCCCCCHHHHCCCCCCEEEEECHHHHH
TIVSQVRRAVPKDIPVTAKMRLGYENTDLALDCARALADGGAEQIVVHARTKVDGYKPPA
HHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCH
HWEWIARIQEVVKVPVVANGEIWTVEDWRRCREICGARDIMIGRGLVARPDLARQIAAAQ
HHHHHHHHHHHHHCCEEECCCEEEHHHHHHHHHHCCCCCEECCCCCCCCCHHHHHHHHHH
KGEEVVPMTWDALQPMLRVFWQDCLVKMTLIQAPGRLKQWLVLLTKSYPEATLMFNTLRR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHC
ETDCDRISVLLGCAPGCLPKS
CCCHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MQIALAPMEGLVDDILRNVLTKVGGIDWCVTEFIRVSERLMPAHYFYKYASEFHNGAKTD
CEEEECHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
AGTPLRLQLLGSDPVCLAENAAFACELGAPVLDLNFGCPAKTVNRSRGGAVLLKEPELLH
CCCCEEEEEECCCCEEEECCCCEEEECCCCEEECCCCCCHHHHCCCCCCEEEEECHHHHH
TIVSQVRRAVPKDIPVTAKMRLGYENTDLALDCARALADGGAEQIVVHARTKVDGYKPPA
HHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCH
HWEWIARIQEVVKVPVVANGEIWTVEDWRRCREICGARDIMIGRGLVARPDLARQIAAAQ
HHHHHHHHHHHHHCCEEECCCEEEHHHHHHHHHHCCCCCEECCCCCCCCCHHHHHHHHHH
KGEEVVPMTWDALQPMLRVFWQDCLVKMTLIQAPGRLKQWLVLLTKSYPEATLMFNTLRR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHC
ETDCDRISVLLGCAPGCLPKS
CCCHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]