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The map label for this gene is dusA [H]

Identifier: 66045176

GI number: 66045176

Start: 2209059

End: 2210078

Strand: Direct

Name: dusA [H]

Synonym: Psyr_1936

Alternate gene names: 66045176

Gene position: 2209059-2210078 (Clockwise)

Preceding gene: 66045174

Following gene: 66045177

Centisome position: 36.25

GC content: 59.31

Gene sequence:

>1020_bases
ATGACACCTGAAAGCCTAGAAACCCCCGTAAATATTGGCCAACCAGCCCCAGTGCTATCCCGCCGCTTCTCCGTCGCCCC
CATGATGGACTGGACCGACCACCACTGCCGCTACTTCATGCGCAAGCTCTCAAAGCATGCCTTGCTCTACACCGAGATGG
TCACCACCGGTGCCTTGCTGCATGGCGACCGCGAGCGGTTCCTGCGCCATGATGAAACCGAGCATCCGCTGGCATTGCAG
TTGGGTGGCAGCACGGCGGCGGATCTGGCTGCGTGTTCGCGTCTGGCGCAAGAGGCGGGGTATGACGAGGTCAATCTGAA
CGTCGGTTGCCCGAGTGACCGGGTGCAGAACAACATGATCGGTGCGTGCCTGATGGCGCATCCTGAGCTGGTGGCGGATT
GCGTGAAGGCGATGCGCGATGCGGTCGGTATTCCGGTCACGGTCAAGCACCGCATCGGTATCAACGGCCGTGACAGCTAC
GCCGAGTTGTGTGATTTCGTCGGCAAGGTTCAGGAGGCGGGTTGCGAGAGTTTCACAGTTCATGCGCGTATCGCGATTCT
CGAAGGGCTGTCTCCCAAGGAAAACCGCGATATTCCGCCACTGCGTTATGACGTGGTCGCGCAGTTGAAGTCTGACTTTC
CCGGCCTGGAGATTGTGCTCAATGGCGGCATCAAGACGCTGGAGCAGTGCAGTGAGCATTTGCAGACGTTCGATGGCGTG
ATGTTGGGGCGTGAGGCTTATCACAACCCGTATCTGTTAGCGCATGTCGATCAGCAGTTGTTCGGCAGCACTGCGCCGGT
CATCAGTCGTTACGATGCGTTGGAGTCGATGCGGCCGTACATTGCGGCGCACATTGCCAGCGGCGGCAACATGCACCACG
TCACCCGGCATATGCTCGGTCTTGGTCTGGGTTTCCCTGGCGCGCGACGCTTCCGTCAGTTGCTGTCGGTTGACATACAC
AAGGCCGAAAATCCCATGCTGTTGCTGGATCAGGCCGCGGCGTTTCTTCAGGGGCATTGA

Upstream 100 bases:

>100_bases
TCGAACAGCTACATTTACAGATCAATTGCTACATCGTGGCAATCGCTTGCTCAAAACGAACAAGCCGGGCTAAAAACAGC
TGCACTCAAGATCAATAAAC

Downstream 100 bases:

>100_bases
TCGCATGTGCGGTGCGTTGATCTGAGCTGGATCAAAGGAAACGCTTATTCCAGTAGCGGCGTTGTCGGGCTGCGGTTATG
TTGAGTGCATAACCCAACAA

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MTPESLETPVNIGQPAPVLSRRFSVAPMMDWTDHHCRYFMRKLSKHALLYTEMVTTGALLHGDRERFLRHDETEHPLALQ
LGGSTAADLAACSRLAQEAGYDEVNLNVGCPSDRVQNNMIGACLMAHPELVADCVKAMRDAVGIPVTVKHRIGINGRDSY
AELCDFVGKVQEAGCESFTVHARIAILEGLSPKENRDIPPLRYDVVAQLKSDFPGLEIVLNGGIKTLEQCSEHLQTFDGV
MLGREAYHNPYLLAHVDQQLFGSTAPVISRYDALESMRPYIAAHIASGGNMHHVTRHMLGLGLGFPGARRFRQLLSVDIH
KAENPMLLLDQAAAFLQGH

Sequences:

>Translated_339_residues
MTPESLETPVNIGQPAPVLSRRFSVAPMMDWTDHHCRYFMRKLSKHALLYTEMVTTGALLHGDRERFLRHDETEHPLALQ
LGGSTAADLAACSRLAQEAGYDEVNLNVGCPSDRVQNNMIGACLMAHPELVADCVKAMRDAVGIPVTVKHRIGINGRDSY
AELCDFVGKVQEAGCESFTVHARIAILEGLSPKENRDIPPLRYDVVAQLKSDFPGLEIVLNGGIKTLEQCSEHLQTFDGV
MLGREAYHNPYLLAHVDQQLFGSTAPVISRYDALESMRPYIAAHIASGGNMHHVTRHMLGLGLGFPGARRFRQLLSVDIH
KAENPMLLLDQAAAFLQGH
>Mature_338_residues
TPESLETPVNIGQPAPVLSRRFSVAPMMDWTDHHCRYFMRKLSKHALLYTEMVTTGALLHGDRERFLRHDETEHPLALQL
GGSTAADLAACSRLAQEAGYDEVNLNVGCPSDRVQNNMIGACLMAHPELVADCVKAMRDAVGIPVTVKHRIGINGRDSYA
ELCDFVGKVQEAGCESFTVHARIAILEGLSPKENRDIPPLRYDVVAQLKSDFPGLEIVLNGGIKTLEQCSEHLQTFDGVM
LGREAYHNPYLLAHVDQQLFGSTAPVISRYDALESMRPYIAAHIASGGNMHHVTRHMLGLGLGFPGARRFRQLLSVDIHK
AENPMLLLDQAAAFLQGH

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=270, Percent_Identity=27.7777777777778, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI239788483, Length=258, Percent_Identity=24.8062015503876, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI239788462, Length=258, Percent_Identity=24.8062015503876, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI145693211, Length=314, Percent_Identity=59.8726114649682, Blast_Score=382, Evalue=1e-107,
Organism=Escherichia coli, GI1789660, Length=248, Percent_Identity=23.3870967741935, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788462, Length=223, Percent_Identity=27.3542600896861, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17507177, Length=284, Percent_Identity=25.3521126760563, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323560, Length=273, Percent_Identity=23.0769230769231, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 37378; Mature: 37247

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPESLETPVNIGQPAPVLSRRFSVAPMMDWTDHHCRYFMRKLSKHALLYTEMVTTGALL
CCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HGDRERFLRHDETEHPLALQLGGSTAADLAACSRLAQEAGYDEVNLNVGCPSDRVQNNMI
CCCHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHH
GACLMAHPELVADCVKAMRDAVGIPVTVKHRIGINGRDSYAELCDFVGKVQEAGCESFTV
HHHHHCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEH
HARIAILEGLSPKENRDIPPLRYDVVAQLKSDFPGLEIVLNGGIKTLEQCSEHLQTFDGV
HEEEHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHH
MLGREAYHNPYLLAHVDQQLFGSTAPVISRYDALESMRPYIAAHIASGGNMHHVTRHMLG
HHHHHHHCCCEEEEECCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LGLGFPGARRFRQLLSVDIHKAENPMLLLDQAAAFLQGH
HCCCCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCC
>Mature Secondary Structure 
TPESLETPVNIGQPAPVLSRRFSVAPMMDWTDHHCRYFMRKLSKHALLYTEMVTTGALL
CCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HGDRERFLRHDETEHPLALQLGGSTAADLAACSRLAQEAGYDEVNLNVGCPSDRVQNNMI
CCCHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHH
GACLMAHPELVADCVKAMRDAVGIPVTVKHRIGINGRDSYAELCDFVGKVQEAGCESFTV
HHHHHCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEH
HARIAILEGLSPKENRDIPPLRYDVVAQLKSDFPGLEIVLNGGIKTLEQCSEHLQTFDGV
HEEEHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHH
MLGREAYHNPYLLAHVDQQLFGSTAPVISRYDALESMRPYIAAHIASGGNMHHVTRHMLG
HHHHHHHCCCEEEEECCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LGLGFPGARRFRQLLSVDIHKAENPMLLLDQAAAFLQGH
HCCCCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]