Click here to switch to the map view.

The map label for this gene is thiF [C]

Identifier: 66045163

GI number: 66045163

Start: 2195237

End: 2196166

Strand: Direct

Name: thiF [C]

Synonym: Psyr_1922

Alternate gene names: 66045163

Gene position: 2195237-2196166 (Clockwise)

Preceding gene: 66045162

Following gene: 66045164

Centisome position: 36.02

GC content: 53.98

Gene sequence:

>930_bases
ATGTTGAAGAAAGCACGCATTGTTAAATTTCCAGGCGTTTTCGACGAGCGGGTATACAACGAGCGGGTTTCACGCAGCGG
TTGTTTTCTCGGCAACTCGAAACTGGAGCAGATGGCCAATCAGGGTAAGCTTTCCAATGCTGTTGTCGGCGTCGCAGGCG
CAGGGGGTATTGGCGGTGCCGTGGCCCTCGGGCTGGCACGGTTGGGTGTAAGGCATATCAAAATTGCCGATCCCGATTCT
TTCGAAATCAGCAATATCAACCGACAGTTTGGTGCTTCAGTGGAAACCGTCGGTCGTAACAAGGCGTTGGTGGTTGCCGA
GCTGGTTCACCGTTTGGCGGGGGACGTGAGCGTTGATGTGTTTCCGGAGGGTATACAGAAGCACACCGCTGAAGCGTTTG
TGGAGGACTGTGATCTGGTGTTGGATCAAATGGACTTCTACAGCATTGGCGAGCGCTATGCACTGCACAGGGCGTACCGT
AACTCAGCCAGGTGCAAAGGCTTGCTGGCATCCTCGGTGGTCGGTTGGGGCGCGAACATCTACAAGTTCGACAAGGCAGG
CCTGACGCTGGAGGATTTTTACGATATTCCGGAAGAGGCGCAGATGACACCTGAACTGGTCGAAAGGCTGGTCATGCTCC
AGGCCTCTTATCAGCCTCGTTTTCCGAGCATTCCCGATATTTTCAGCTGGATGAAAGAGACAGGAAACGTGCCTATTCTT
TCCGTGGCACCGACGGCTTCGCATTACCTGATTCTCTGTCGTTCAGCGCTGATGCTTTGCGACCTGGAGGGCGCACCGTA
CAGCACCGAACTTCCTCCCATGCCCAAGTATTACTGGTTCGACGGCTCTACGTTCGACAACGGTATTTATGAATTCGACG
GCACGTGGGCAAACCCCGAGGAGCATCGCAAGCACTTCAATGTCAATTGA

Upstream 100 bases:

>100_bases
ACTCTATCGCGAAGTACGGGTTTTTCTGGAAGGCTCATATAACCGATTGTCACTGGCCTGTAGTTTTTGCGGCGGCCGCA
ACTAAAGCAAGGAGCAGATC

Downstream 100 bases:

>100_bases
ACCAGCTGGCCCCGATCGGGGCCAGCCCTTTGAGGAAGGCGCCGTGAACGAAGTGCTCGATACTCAGGAACGGCACAATA
TTTCCAGGTTCCTCCTGGCC

Product: UBA/THIF-type NAD/FAD binding fold

Products: ThiS-COSH; AMP; ThiS-COAMP; pyrophosphate [C]

Alternate protein names: Molybdopterin/Thiamine Biosynthesis Family Protein; Molybdopterin Biosynthesis Protein MoeW

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MLKKARIVKFPGVFDERVYNERVSRSGCFLGNSKLEQMANQGKLSNAVVGVAGAGGIGGAVALGLARLGVRHIKIADPDS
FEISNINRQFGASVETVGRNKALVVAELVHRLAGDVSVDVFPEGIQKHTAEAFVEDCDLVLDQMDFYSIGERYALHRAYR
NSARCKGLLASSVVGWGANIYKFDKAGLTLEDFYDIPEEAQMTPELVERLVMLQASYQPRFPSIPDIFSWMKETGNVPIL
SVAPTASHYLILCRSALMLCDLEGAPYSTELPPMPKYYWFDGSTFDNGIYEFDGTWANPEEHRKHFNVN

Sequences:

>Translated_309_residues
MLKKARIVKFPGVFDERVYNERVSRSGCFLGNSKLEQMANQGKLSNAVVGVAGAGGIGGAVALGLARLGVRHIKIADPDS
FEISNINRQFGASVETVGRNKALVVAELVHRLAGDVSVDVFPEGIQKHTAEAFVEDCDLVLDQMDFYSIGERYALHRAYR
NSARCKGLLASSVVGWGANIYKFDKAGLTLEDFYDIPEEAQMTPELVERLVMLQASYQPRFPSIPDIFSWMKETGNVPIL
SVAPTASHYLILCRSALMLCDLEGAPYSTELPPMPKYYWFDGSTFDNGIYEFDGTWANPEEHRKHFNVN
>Mature_309_residues
MLKKARIVKFPGVFDERVYNERVSRSGCFLGNSKLEQMANQGKLSNAVVGVAGAGGIGGAVALGLARLGVRHIKIADPDS
FEISNINRQFGASVETVGRNKALVVAELVHRLAGDVSVDVFPEGIQKHTAEAFVEDCDLVLDQMDFYSIGERYALHRAYR
NSARCKGLLASSVVGWGANIYKFDKAGLTLEDFYDIPEEAQMTPELVERLVMLQASYQPRFPSIPDIFSWMKETGNVPIL
SVAPTASHYLILCRSALMLCDLEGAPYSTELPPMPKYYWFDGSTFDNGIYEFDGTWANPEEHRKHFNVN

Specific function: Catalyzes The Adenylation By ATP Of The Carboxyl- Terminal Glycine Of This. [C]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.7.- [C]

Molecular weight: Translated: 34253; Mature: 34253

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKARIVKFPGVFDERVYNERVSRSGCFLGNSKLEQMANQGKLSNAVVGVAGAGGIGGA
CCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCEEEECCCCCCHHH
VALGLARLGVRHIKIADPDSFEISNINRQFGASVETVGRNKALVVAELVHRLAGDVSVDV
HHHHHHHHCCEEEEECCCCCCCCCCCCHHHCCCHHHCCCCCCHHHHHHHHHHHCCCEEEE
FPEGIQKHTAEAFVEDCDLVLDQMDFYSIGERYALHRAYRNSARCKGLLASSVVGWGANI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
YKFDKAGLTLEDFYDIPEEAQMTPELVERLVMLQASYQPRFPSIPDIFSWMKETGNVPIL
EEECCCCCCHHHHHCCCHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEE
SVAPTASHYLILCRSALMLCDLEGAPYSTELPPMPKYYWFDGSTFDNGIYEFDGTWANPE
EECCCHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCEEEECCCCCCCCEEEECCCCCCHH
EHRKHFNVN
HHHHCCCCC
>Mature Secondary Structure
MLKKARIVKFPGVFDERVYNERVSRSGCFLGNSKLEQMANQGKLSNAVVGVAGAGGIGGA
CCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCEEEECCCCCCHHH
VALGLARLGVRHIKIADPDSFEISNINRQFGASVETVGRNKALVVAELVHRLAGDVSVDV
HHHHHHHHCCEEEEECCCCCCCCCCCCHHHCCCHHHCCCCCCHHHHHHHHHHHCCCEEEE
FPEGIQKHTAEAFVEDCDLVLDQMDFYSIGERYALHRAYRNSARCKGLLASSVVGWGANI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
YKFDKAGLTLEDFYDIPEEAQMTPELVERLVMLQASYQPRFPSIPDIFSWMKETGNVPIL
EEECCCCCCHHHHHCCCHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEE
SVAPTASHYLILCRSALMLCDLEGAPYSTELPPMPKYYWFDGSTFDNGIYEFDGTWANPE
EECCCHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCEEEECCCCCCCCEEEECCCCCCHH
EHRKHFNVN
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine; ThiS protein; ATP [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP ThiS protein + ATP = ThiS-COAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA