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The map label for this gene is cpg2 [H]

Identifier: 66045156

GI number: 66045156

Start: 2189228

End: 2190526

Strand: Direct

Name: cpg2 [H]

Synonym: Psyr_1915

Alternate gene names: 66045156

Gene position: 2189228-2190526 (Clockwise)

Preceding gene: 66045154

Following gene: 66045157

Centisome position: 35.93

GC content: 60.89

Gene sequence:

>1299_bases
ATGCTGTTCAACTCGCGATCATCTGAAGGAACCAGCATGCATTCACGACGCTTCCCCATCACTGCCGCAATCGGACTTTA
CCTGGTGTCCATGGGCGTGTTTTCCATGGTTGCCGACGCCGCCGAGCTGTCACCCGATCAGTTGCTGAAAAAAGCCCAGG
CCGAACAACCGGCCTACCTCGACACTGTCCGCCAGCTCGTTGATGTCGATACAGGCACCGGCCAGGCTCCCGGGCTGAAG
ACCGTCAGCGCAATGCTGGTCGAACGTCTCAAGGCGCTGGGTGCAGAAGTCTCCACCACGCCGGCAGAGCCTTCGGCGGG
GGACAATATCGTCGGTACTTTCAAAGGCACCGGCACACGGTCTTTTCTGTTGATGGTTCACTACGACACGGTGTTCGGGC
CGGGCACCGCCGCCAAGCGTCCGTTCAGACTCGACAGCGAGCGCGCCTATGGCCCAGGGGTGGCAGACGCCAAGGGCGGC
GTGGCGATGATTCTGCATGCGCTGCAGCTGTTGCAGAATGAGCGGTTCAAGGCGTTCGGCACGCTCACTGTGCTCTTCAA
CCCGGACGAGGAGACCGGCTCCAGCGGCTCGAAAAAAGTCATCGCCGAACTCGCCCGCCAGCATGACTACGTGTTTTCGT
TCGAGCCGCCTGACAAGGATGCCGTCACCGTCGCCACCAACGGCATCAATGGCCTGATTCTCGAGGTCAAAGGCAAGTCG
TCACACGCAGGCTCTGCTCCCGAGGCCGGACGCAATGCGGCCATCGAGCTGGCGCACCAACTGCTGCAGCTCAAGGACCT
CGGCGACCCGGGCAAAGGCACCACTGTCAACTGGACGCTGATCAAGGGCGGGGAGAAGCGCAACATCATTCCATCCAGCG
CTTCGGCCGAGGCGGACATGCGCTATTCCGACCTCAGCGAGAGCGATCGCGTATTGGCTGACGGACAAAAGATCGTGAAG
AAGACGCTGGTCGATGGCACCGAAGTGACCTTGCGAATGGAAAAAGGCCGGCCGCCGCTGGCCAGAAAGCCCGGCTCCGA
CCAGTTGGCGAAAACCGCGCAGACGCTTTACCAGAAAATTGGCCGCAGCGTGGAGCCGATTGCCATGCGTTTCGGTACCG
ACGCTGGCTATGCCTACGTGCCGGGCAGTGCAAAGCCTGCGGTGCTCGAAACCATGGGCGTGGTCGGTGCCGGCCTGCAT
GCCGACGATGAATACATCGAATTATCGAGCATCGCACCCAGGCTTTACCTGACCGTGGCGTTGATTACGCAGTTGTCGAA
GGGTGATGCAACGCCCTGA

Upstream 100 bases:

>100_bases
TATCAGGCCATCGGGCCTCTGGCAGCAGACATTTGCCTTGATATGAGCCGGTCTCGCAACCGGTCTGGCCTGTGGCGTCG
AGCGGCGCATTGGGGTAAAG

Downstream 100 bases:

>100_bases
TGGCCTGTGAGGTGAGCGCTGAAAGGCGTGGTCTTCGGTCCGCGCCATCTGTCGAGGTATCTGGCATTGCGATTGACCTG
AGCGCCGATAAACGATTAAC

Product: glutamate carboxypeptidase

Products: NA

Alternate protein names: CPDG2; Folate hydrolase G2; Glutamate carboxypeptidase; Pteroylmonoglutamic acid hydrolase G2; Glucarpidase [H]

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MLFNSRSSEGTSMHSRRFPITAAIGLYLVSMGVFSMVADAAELSPDQLLKKAQAEQPAYLDTVRQLVDVDTGTGQAPGLK
TVSAMLVERLKALGAEVSTTPAEPSAGDNIVGTFKGTGTRSFLLMVHYDTVFGPGTAAKRPFRLDSERAYGPGVADAKGG
VAMILHALQLLQNERFKAFGTLTVLFNPDEETGSSGSKKVIAELARQHDYVFSFEPPDKDAVTVATNGINGLILEVKGKS
SHAGSAPEAGRNAAIELAHQLLQLKDLGDPGKGTTVNWTLIKGGEKRNIIPSSASAEADMRYSDLSESDRVLADGQKIVK
KTLVDGTEVTLRMEKGRPPLARKPGSDQLAKTAQTLYQKIGRSVEPIAMRFGTDAGYAYVPGSAKPAVLETMGVVGAGLH
ADDEYIELSSIAPRLYLTVALITQLSKGDATP

Sequences:

>Translated_432_residues
MLFNSRSSEGTSMHSRRFPITAAIGLYLVSMGVFSMVADAAELSPDQLLKKAQAEQPAYLDTVRQLVDVDTGTGQAPGLK
TVSAMLVERLKALGAEVSTTPAEPSAGDNIVGTFKGTGTRSFLLMVHYDTVFGPGTAAKRPFRLDSERAYGPGVADAKGG
VAMILHALQLLQNERFKAFGTLTVLFNPDEETGSSGSKKVIAELARQHDYVFSFEPPDKDAVTVATNGINGLILEVKGKS
SHAGSAPEAGRNAAIELAHQLLQLKDLGDPGKGTTVNWTLIKGGEKRNIIPSSASAEADMRYSDLSESDRVLADGQKIVK
KTLVDGTEVTLRMEKGRPPLARKPGSDQLAKTAQTLYQKIGRSVEPIAMRFGTDAGYAYVPGSAKPAVLETMGVVGAGLH
ADDEYIELSSIAPRLYLTVALITQLSKGDATP
>Mature_432_residues
MLFNSRSSEGTSMHSRRFPITAAIGLYLVSMGVFSMVADAAELSPDQLLKKAQAEQPAYLDTVRQLVDVDTGTGQAPGLK
TVSAMLVERLKALGAEVSTTPAEPSAGDNIVGTFKGTGTRSFLLMVHYDTVFGPGTAAKRPFRLDSERAYGPGVADAKGG
VAMILHALQLLQNERFKAFGTLTVLFNPDEETGSSGSKKVIAELARQHDYVFSFEPPDKDAVTVATNGINGLILEVKGKS
SHAGSAPEAGRNAAIELAHQLLQLKDLGDPGKGTTVNWTLIKGGEKRNIIPSSASAEADMRYSDLSESDRVLADGQKIVK
KTLVDGTEVTLRMEKGRPPLARKPGSDQLAKTAQTLYQKIGRSVEPIAMRFGTDAGYAYVPGSAKPAVLETMGVVGAGLH
ADDEYIELSSIAPRLYLTVALITQLSKGDATP

Specific function: Catalyzes the hydrolysis of reduced and non-reduced folates to pteroates and L-glutamate. This enzyme has a broad specificity [H]

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family [H]

Homologues:

Organism=Escherichia coli, GI1790395, Length=396, Percent_Identity=27.2727272727273, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR017150
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.4.17.11 [H]

Molecular weight: Translated: 45863; Mature: 45863

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFNSRSSEGTSMHSRRFPITAAIGLYLVSMGVFSMVADAAELSPDQLLKKAQAEQPAYL
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHH
DTVRQLVDVDTGTGQAPGLKTVSAMLVERLKALGAEVSTTPAEPSAGDNIVGTFKGTGTR
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCC
SFLLMVHYDTVFGPGTAAKRPFRLDSERAYGPGVADAKGGVAMILHALQLLQNERFKAFG
EEEEEEEECCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEE
TLTVLFNPDEETGSSGSKKVIAELARQHDYVFSFEPPDKDAVTVATNGINGLILEVKGKS
EEEEEECCCCCCCCCCHHHHHHHHHHHCCEEEEECCCCCCEEEEEECCCCEEEEEEECCC
SHAGSAPEAGRNAAIELAHQLLQLKDLGDPGKGTTVNWTLIKGGEKRNIIPSSASAEADM
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCC
RYSDLSESDRVLADGQKIVKKTLVDGTEVTLRMEKGRPPLARKPGSDQLAKTAQTLYQKI
CHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHH
GRSVEPIAMRFGTDAGYAYVPGSAKPAVLETMGVVGAGLHADDEYIELSSIAPRLYLTVA
CCCCCCHHHEECCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCEEEHHHHCHHHHHHHH
LITQLSKGDATP
HHHHHCCCCCCC
>Mature Secondary Structure
MLFNSRSSEGTSMHSRRFPITAAIGLYLVSMGVFSMVADAAELSPDQLLKKAQAEQPAYL
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHH
DTVRQLVDVDTGTGQAPGLKTVSAMLVERLKALGAEVSTTPAEPSAGDNIVGTFKGTGTR
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCC
SFLLMVHYDTVFGPGTAAKRPFRLDSERAYGPGVADAKGGVAMILHALQLLQNERFKAFG
EEEEEEEECCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEE
TLTVLFNPDEETGSSGSKKVIAELARQHDYVFSFEPPDKDAVTVATNGINGLILEVKGKS
EEEEEECCCCCCCCCCHHHHHHHHHHHCCEEEEECCCCCCEEEEEECCCCEEEEEEECCC
SHAGSAPEAGRNAAIELAHQLLQLKDLGDPGKGTTVNWTLIKGGEKRNIIPSSASAEADM
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCC
RYSDLSESDRVLADGQKIVKKTLVDGTEVTLRMEKGRPPLARKPGSDQLAKTAQTLYQKI
CHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHH
GRSVEPIAMRFGTDAGYAYVPGSAKPAVLETMGVVGAGLHADDEYIELSSIAPRLYLTVA
CCCCCCHHHEECCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCEEEHHHHCHHHHHHHH
LITQLSKGDATP
HHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6396165; 3838935; 9083113 [H]