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The map label for this gene is citA [H]

Identifier: 66045140

GI number: 66045140

Start: 2173554

End: 2174846

Strand: Reverse

Name: citA [H]

Synonym: Psyr_1899

Alternate gene names: 66045140

Gene position: 2174846-2173554 (Counterclockwise)

Preceding gene: 66045143

Following gene: 66045137

Centisome position: 35.69

GC content: 61.25

Gene sequence:

>1293_bases
ATGGCCTCCACAACAAGTAAGGGCAAAGCGATCTTTCGCGTTGTCAGCGGTAACTTCCTCGAGATGTTCGATTTCATGGT
CTACGGCTTCTATGCCACGGCCATCGCAAAAACCTTCTTCCCTTCCGACAGCGCTTTCGCTTCGTTGATGCTGTCGCTGG
CGACCTTCGGCGCAGGCTTCCTGATGCGTCCGCTGGGCGCAATCTTTCTCGGTGCCTACATCGACCGTCACGGGCGTCGC
AAAGGGCTGATTATCACCCTCGCGATGATGGCAATGGGCACTCTGTTGATTGCCTGCGTGCCGGGCTACTCCACATTGGG
CGTCATCGCCCCCTTGCTGGTGCTGCTCGGTCGTTTGCTGCAAGGTTTCTCGGCAGGTGTGGAACTGGGTGGTGTTTCGG
TGTACCTGGCTGAAATCTCGACACCGGGCCGCAAAGGCTTCTTCGTCAGCTGGCAGTCCGCCAGCCAACAGGCCGCCGTG
GTCTTCGCCGGCTTGCTGGGCGTGGGTCTCAACCACTGGCTGAGCCCGGAGCAGATGGGTGAATGGGGCTGGCGCGTGCC
GTTCCTGATCGGCTGCCTGATCGTGCCGGCCATCTTCATCATCCGTCGCTCGCTGGAAGAGTCGCCGGAGTTCGAAGCCC
GCACGCATCGCCCGACGCTGAGTGAAGTGGTGCGGTCTATCGGCCAGAACTTCGGTCTGGTGCTGGGCGGCATGGCGCTG
GTGGTCATGACCACCGTGACGTTCTACCTGATCACCGCTTACACCCCGACCTTCGGCAAGAACGAGTTGAACCTGACAGA
CCTGGAAAGCCTGCTGGTGACGGTCTGCGTTGGTGTGTCCAACTTCATCTGGCTGCCGATCATGGGCTCGTTCTCGGACC
GCATCGGCCGCAAGCCGCTGCTGATTGCGGCGACCGTACTGGCAATCGCCACTGCCTACCCTGCTCTTTCGTGGCTGGTT
GCGCACCCGAGCTTCGGCAACCTGCTGATGGTCGAGTTGTGGCTGTCGTTCCTGTATGGCTCCTACAACGGGGCAATGGT
CGTGGCGCTGACCGAGATCATGCCGGTCGACGTCCGCACCACCGGCTTCTCGCTGGCCTACAGCCTGGCGACTGCGACCT
TCGGTGGTTTTACCCCGGCGGCCTGTACTTACCTGATTCACGTACTGGACAACAAGGCAGCGCCAGGTATCTGGCTAACC
GGCGCAGCGGTGCTGGGTCTGGTTGCGACGCTGATACTGTTCCGCAAGGGTGGGCAGAAGCTTGAAGCGCCTGTCGCGGC
TTCCGCCAGCTGA

Upstream 100 bases:

>100_bases
GTTACAAGTGCGCATCGCCAGTTGCACACACGAAATGGCGATGCGAACGGGATAAAAACGACAACGGACTCCAAGCCGAC
GAGCCTGAGCGAGTAACTAA

Downstream 100 bases:

>100_bases
TCGATCGCGGCATTGCAACAAGAAAACCCCGGCTCGAGAGTCTCTCGGCCGGGGTTTTGCGTTTTCAGACCTTGCGTTTC
CAGGGCCGAAAGCTGAGCCA

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter; Citrate utilization determinant; Citrate utilization protein A [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MASTTSKGKAIFRVVSGNFLEMFDFMVYGFYATAIAKTFFPSDSAFASLMLSLATFGAGFLMRPLGAIFLGAYIDRHGRR
KGLIITLAMMAMGTLLIACVPGYSTLGVIAPLLVLLGRLLQGFSAGVELGGVSVYLAEISTPGRKGFFVSWQSASQQAAV
VFAGLLGVGLNHWLSPEQMGEWGWRVPFLIGCLIVPAIFIIRRSLEESPEFEARTHRPTLSEVVRSIGQNFGLVLGGMAL
VVMTTVTFYLITAYTPTFGKNELNLTDLESLLVTVCVGVSNFIWLPIMGSFSDRIGRKPLLIAATVLAIATAYPALSWLV
AHPSFGNLLMVELWLSFLYGSYNGAMVVALTEIMPVDVRTTGFSLAYSLATATFGGFTPAACTYLIHVLDNKAAPGIWLT
GAAVLGLVATLILFRKGGQKLEAPVAASAS

Sequences:

>Translated_430_residues
MASTTSKGKAIFRVVSGNFLEMFDFMVYGFYATAIAKTFFPSDSAFASLMLSLATFGAGFLMRPLGAIFLGAYIDRHGRR
KGLIITLAMMAMGTLLIACVPGYSTLGVIAPLLVLLGRLLQGFSAGVELGGVSVYLAEISTPGRKGFFVSWQSASQQAAV
VFAGLLGVGLNHWLSPEQMGEWGWRVPFLIGCLIVPAIFIIRRSLEESPEFEARTHRPTLSEVVRSIGQNFGLVLGGMAL
VVMTTVTFYLITAYTPTFGKNELNLTDLESLLVTVCVGVSNFIWLPIMGSFSDRIGRKPLLIAATVLAIATAYPALSWLV
AHPSFGNLLMVELWLSFLYGSYNGAMVVALTEIMPVDVRTTGFSLAYSLATATFGGFTPAACTYLIHVLDNKAAPGIWLT
GAAVLGLVATLILFRKGGQKLEAPVAASAS
>Mature_429_residues
ASTTSKGKAIFRVVSGNFLEMFDFMVYGFYATAIAKTFFPSDSAFASLMLSLATFGAGFLMRPLGAIFLGAYIDRHGRRK
GLIITLAMMAMGTLLIACVPGYSTLGVIAPLLVLLGRLLQGFSAGVELGGVSVYLAEISTPGRKGFFVSWQSASQQAAVV
FAGLLGVGLNHWLSPEQMGEWGWRVPFLIGCLIVPAIFIIRRSLEESPEFEARTHRPTLSEVVRSIGQNFGLVLGGMALV
VMTTVTFYLITAYTPTFGKNELNLTDLESLLVTVCVGVSNFIWLPIMGSFSDRIGRKPLLIAATVLAIATAYPALSWLVA
HPSFGNLLMVELWLSFLYGSYNGAMVVALTEIMPVDVRTTGFSLAYSLATATFGGFTPAACTYLIHVLDNKAAPGIWLTG
AAVLGLVATLILFRKGGQKLEAPVAASAS

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=435, Percent_Identity=29.8850574712644, Blast_Score=211, Evalue=7e-56,
Organism=Escherichia coli, GI1788942, Length=424, Percent_Identity=34.4339622641509, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1788292, Length=436, Percent_Identity=29.1284403669725, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI1789941, Length=427, Percent_Identity=29.2740046838407, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI87082231, Length=206, Percent_Identity=28.1553398058252, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 45978; Mature: 45847

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASTTSKGKAIFRVVSGNFLEMFDFMVYGFYATAIAKTFFPSDSAFASLMLSLATFGAGF
CCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHH
LMRPLGAIFLGAYIDRHGRRKGLIITLAMMAMGTLLIACVPGYSTLGVIAPLLVLLGRLL
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
QGFSAGVELGGVSVYLAEISTPGRKGFFVSWQSASQQAAVVFAGLLGVGLNHWLSPEQMG
HHHHCCCEECCEEEEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHC
EWGWRVPFLIGCLIVPAIFIIRRSLEESPEFEARTHRPTLSEVVRSIGQNFGLVLGGMAL
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHH
VVMTTVTFYLITAYTPTFGKNELNLTDLESLLVTVCVGVSNFIWLPIMGSFSDRIGRKPL
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHCCCHH
LIAATVLAIATAYPALSWLVAHPSFGNLLMVELWLSFLYGSYNGAMVVALTEIMPVDVRT
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEHHCCCCEEC
TGFSLAYSLATATFGGFTPAACTYLIHVLDNKAAPGIWLTGAAVLGLVATLILFRKGGQK
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCC
LEAPVAASAS
CCCCCCCCCC
>Mature Secondary Structure 
ASTTSKGKAIFRVVSGNFLEMFDFMVYGFYATAIAKTFFPSDSAFASLMLSLATFGAGF
CCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHH
LMRPLGAIFLGAYIDRHGRRKGLIITLAMMAMGTLLIACVPGYSTLGVIAPLLVLLGRLL
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
QGFSAGVELGGVSVYLAEISTPGRKGFFVSWQSASQQAAVVFAGLLGVGLNHWLSPEQMG
HHHHCCCEECCEEEEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHC
EWGWRVPFLIGCLIVPAIFIIRRSLEESPEFEARTHRPTLSEVVRSIGQNFGLVLGGMAL
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHH
VVMTTVTFYLITAYTPTFGKNELNLTDLESLLVTVCVGVSNFIWLPIMGSFSDRIGRKPL
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHCCCHH
LIAATVLAIATAYPALSWLVAHPSFGNLLMVELWLSFLYGSYNGAMVVALTEIMPVDVRT
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEHHCCCCEEC
TGFSLAYSLATATFGGFTPAACTYLIHVLDNKAAPGIWLTGAAVLGLVATLILFRKGGQK
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCC
LEAPVAASAS
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]