Click here to switch to the map view.

The map label for this gene is speE [H]

Identifier: 66045108

GI number: 66045108

Start: 2139549

End: 2140409

Strand: Direct

Name: speE [H]

Synonym: Psyr_1864

Alternate gene names: 66045108

Gene position: 2139549-2140409 (Clockwise)

Preceding gene: 66045107

Following gene: 66045109

Centisome position: 35.11

GC content: 57.14

Gene sequence:

>861_bases
ATGAGCGATTATCAGGAAACGTTATACGAAGGCTACGGCCAACGCTTTCGTATGGAAAAAATGCTTCACGAAGTACGTAC
CGATCACCAGCACCTGGTGATCTTCCAGAACCCGCGCATGGGCCGTGTCATGGCGCTGGACGGCGTCATTCAGACCACCG
AAGCGGACGAATTCATCTACCACGAAATGCTCACCCATGTGCCGATTCTGGCGCACGGTGCAGCCAAGCGCGTACTGATC
ATCGGCGGTGGCGACGGCGGCATGCTGCGTGAAGTCGCCAAGCACATGAGCGTCGAACATATCACCATGGTCGAGATCGA
TGCGACCGTGGTCGAGATGTGCAAGGAATATCTGCCCAATCATTCCAGCGGTGCCTTCGACGATTCGCGTCTGAACCTGG
TGATCGACGACGGCATGCGCTTCGTCGCGACCACCGAAGAGAAGTTCGACGTGATCATCTCCGACTCCACCGATCCGATC
GGCCCGGGTGAAGTCCTGTTCTCGGAAAACTTTTATCAGGCCTGCCATCGCTGCCTCAATGAAGGCGGCATTCTGGTCAC
CCAGAACGGCACGCCTTTCATGCAGTTGAGCGGTGTGCAGACCACGGCCGGGCGCATGAACGGTCTGTTTGCCGACTGGC
ATTTCTATCACGCAGCGATCCCGACCTACATTGGCGGCGCAATGACTTTCGCCTGGGGTTCGACCGACAAGAGCTATCGC
AAACTGCCGCTGGAAACCCTGCGTCAGCGTTTCTCGGGCAGCGGTATTGTTACGCGTTATTACAATCCGGAAGTCCACAT
CGGCGCATTCGCCTTGCCACAGTACGTTCTGCAGGCCGTAGGCAAGGCCAGCAACGACTGA

Upstream 100 bases:

>100_bases
TGCATAGAGGTTCGACCTGTCATGTTCCTCGACGTAGAATGCGCGCCTTTGTTGCGGGCGGTCGGCCGTCCTGTCATCAG
TACCCAGCGGGAGCTTACCC

Downstream 100 bases:

>100_bases
TGAGTCGGTCCGGCTCCTCGAGCCGGATTTTCTCGAGCGTGGCCTGTGAGGCTTTATAAGCGCTTAATTCGCGACAGAAA
TTTCACACGGTCTGTCACAA

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY [H]

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MSDYQETLYEGYGQRFRMEKMLHEVRTDHQHLVIFQNPRMGRVMALDGVIQTTEADEFIYHEMLTHVPILAHGAAKRVLI
IGGGDGGMLREVAKHMSVEHITMVEIDATVVEMCKEYLPNHSSGAFDDSRLNLVIDDGMRFVATTEEKFDVIISDSTDPI
GPGEVLFSENFYQACHRCLNEGGILVTQNGTPFMQLSGVQTTAGRMNGLFADWHFYHAAIPTYIGGAMTFAWGSTDKSYR
KLPLETLRQRFSGSGIVTRYYNPEVHIGAFALPQYVLQAVGKASND

Sequences:

>Translated_286_residues
MSDYQETLYEGYGQRFRMEKMLHEVRTDHQHLVIFQNPRMGRVMALDGVIQTTEADEFIYHEMLTHVPILAHGAAKRVLI
IGGGDGGMLREVAKHMSVEHITMVEIDATVVEMCKEYLPNHSSGAFDDSRLNLVIDDGMRFVATTEEKFDVIISDSTDPI
GPGEVLFSENFYQACHRCLNEGGILVTQNGTPFMQLSGVQTTAGRMNGLFADWHFYHAAIPTYIGGAMTFAWGSTDKSYR
KLPLETLRQRFSGSGIVTRYYNPEVHIGAFALPQYVLQAVGKASND
>Mature_285_residues
SDYQETLYEGYGQRFRMEKMLHEVRTDHQHLVIFQNPRMGRVMALDGVIQTTEADEFIYHEMLTHVPILAHGAAKRVLII
GGGDGGMLREVAKHMSVEHITMVEIDATVVEMCKEYLPNHSSGAFDDSRLNLVIDDGMRFVATTEEKFDVIISDSTDPIG
PGEVLFSENFYQACHRCLNEGGILVTQNGTPFMQLSGVQTTAGRMNGLFADWHFYHAAIPTYIGGAMTFAWGSTDKSYRK
LPLETLRQRFSGSGIVTRYYNPEVHIGAFALPQYVLQAVGKASND

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=279, Percent_Identity=37.9928315412186, Blast_Score=211, Evalue=5e-55,
Organism=Escherichia coli, GI1786312, Length=279, Percent_Identity=58.4229390681004, Blast_Score=354, Evalue=5e-99,
Organism=Caenorhabditis elegans, GI25154106, Length=282, Percent_Identity=39.3617021276596, Blast_Score=183, Evalue=7e-47,
Organism=Caenorhabditis elegans, GI212645616, Length=333, Percent_Identity=35.7357357357357, Blast_Score=181, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6323175, Length=279, Percent_Identity=40.1433691756272, Blast_Score=224, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6325326, Length=277, Percent_Identity=35.7400722021661, Blast_Score=196, Evalue=4e-51,
Organism=Drosophila melanogaster, GI24645443, Length=276, Percent_Identity=38.4057971014493, Blast_Score=198, Evalue=3e-51,
Organism=Drosophila melanogaster, GI21355941, Length=214, Percent_Identity=40.1869158878505, Blast_Score=157, Evalue=7e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 32021; Mature: 31890

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1 ; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDYQETLYEGYGQRFRMEKMLHEVRTDHQHLVIFQNPRMGRVMALDGVIQTTEADEFIY
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEECCEEECCCHHHHHH
HEMLTHVPILAHGAAKRVLIIGGGDGGMLREVAKHMSVEHITMVEIDATVVEMCKEYLPN
HHHHHHCCEEECCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHCCC
HSSGAFDDSRLNLVIDDGMRFVATTEEKFDVIISDSTDPIGPGEVLFSENFYQACHRCLN
CCCCCCCCCEEEEEEECCEEEEEECCCCEEEEEECCCCCCCCCCEEECCHHHHHHHHHHC
EGGILVTQNGTPFMQLSGVQTTAGRMNGLFADWHFYHAAIPTYIGGAMTFAWGSTDKSYR
CCCEEEECCCCCEEEECCCCHHHHHHCCEEEHHHHHHHHHHHHHCCCEEEEECCCCCHHH
KLPLETLRQRFSGSGIVTRYYNPEVHIGAFALPQYVLQAVGKASND
CCCHHHHHHHHCCCCEEEEEECCCEEECHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SDYQETLYEGYGQRFRMEKMLHEVRTDHQHLVIFQNPRMGRVMALDGVIQTTEADEFIY
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEECCEEECCCHHHHHH
HEMLTHVPILAHGAAKRVLIIGGGDGGMLREVAKHMSVEHITMVEIDATVVEMCKEYLPN
HHHHHHCCEEECCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHCCC
HSSGAFDDSRLNLVIDDGMRFVATTEEKFDVIISDSTDPIGPGEVLFSENFYQACHRCLN
CCCCCCCCCEEEEEEECCEEEEEECCCCEEEEEECCCCCCCCCCEEECCHHHHHHHHHHC
EGGILVTQNGTPFMQLSGVQTTAGRMNGLFADWHFYHAAIPTYIGGAMTFAWGSTDKSYR
CCCEEEECCCCCEEEECCCCHHHHHHCCEEEHHHHHHHHHHHHHCCCEEEEECCCCCHHH
KLPLETLRQRFSGSGIVTRYYNPEVHIGAFALPQYVLQAVGKASND
CCCHHHHHHHHCCCCEEEEEECCCEEECHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]