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The map label for this gene is miaE [H]

Identifier: 66044973

GI number: 66044973

Start: 1962478

End: 1963089

Strand: Direct

Name: miaE [H]

Synonym: Psyr_1728

Alternate gene names: 66044973

Gene position: 1962478-1963089 (Clockwise)

Preceding gene: 66044972

Following gene: 66044974

Centisome position: 32.21

GC content: 59.97

Gene sequence:

>612_bases
ATGTACCCGATCCCTGAAATCGAAGCCTTCCTGCTTTGCCGCACCCCCGACAGCTGGGTCCAGGCCGCGCTGCGCAATCT
GGACATCCTGCTGATCGACCATGCGAACAACGAGAAGAAGGCCGCCGGGGCCGCGTTCCAGTTCATGTTTCAGTACAACG
ACAAGTTCGACCTGTTGAGCAAGATGTCACGTCTGGCCCGGGAAGAACTGCGCCATTTCGAGCAAGTCATCAGCATCATC
AGAAAGCGCCAGATCCCCATGGCCAATATCAGCTCGGCCCGTTATGCCGGAGCGCTGCGCAAGCTGGTGCGCAATCACAA
CCCGTACCGGCTGACCGATGCGCTGATCGTCGGTGCCATCGTCGAGGCGCGTTCCTGCGAACGCTTCGCCGCGCTGGTGC
CGCATCTGGACGAAGAGCTGGCCAAGTTCTATGGCGGCCTGCTGAAGTCCGAAGCGCGGCACTTTCAGGACTACCTCAAG
CTGGCTTACAGCTACGGCGACAAGGCTGATGTCGATGCCAAGATCGAGGAGATCCGTCTGGCCGAGCGTGAGCTGATCGA
AAGCCCTGACGAAGAGTTCCGCTTTCACAGTGGCGTACCTGTCGCCGCCTGA

Upstream 100 bases:

>100_bases
CGCCTTCGGCGCCTGACAAACCCGGCATGCTTTTGTGTCGCGGGGCATCGTTGCTGCTCGTATAATGCCCGGCCGTGCCT
CTCCGTCATTCTGTGAGCCT

Downstream 100 bases:

>100_bases
TTGCTCTCGCGTTCCCTCCAAAACGACCGCCTTGCCAGCGGTCGTTTTTTTATGGGCATTTAGTAAGCAAGCTAATAAAT
GCTTTGACAACGGCTGCCTA

Product: tRNA--hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MYPIPEIEAFLLCRTPDSWVQAALRNLDILLIDHANNEKKAAGAAFQFMFQYNDKFDLLSKMSRLAREELRHFEQVISII
RKRQIPMANISSARYAGALRKLVRNHNPYRLTDALIVGAIVEARSCERFAALVPHLDEELAKFYGGLLKSEARHFQDYLK
LAYSYGDKADVDAKIEEIRLAERELIESPDEEFRFHSGVPVAA

Sequences:

>Translated_203_residues
MYPIPEIEAFLLCRTPDSWVQAALRNLDILLIDHANNEKKAAGAAFQFMFQYNDKFDLLSKMSRLAREELRHFEQVISII
RKRQIPMANISSARYAGALRKLVRNHNPYRLTDALIVGAIVEARSCERFAALVPHLDEELAKFYGGLLKSEARHFQDYLK
LAYSYGDKADVDAKIEEIRLAERELIESPDEEFRFHSGVPVAA
>Mature_203_residues
MYPIPEIEAFLLCRTPDSWVQAALRNLDILLIDHANNEKKAAGAAFQFMFQYNDKFDLLSKMSRLAREELRHFEQVISII
RKRQIPMANISSARYAGALRKLVRNHNPYRLTDALIVGAIVEARSCERFAALVPHLDEELAKFYGGLLKSEARHFQDYLK
LAYSYGDKADVDAKIEEIRLAERELIESPDEEFRFHSGVPVAA

Specific function: Production of the modified nucleoside 2-methylthio-cis- ribozeatin (MS[2]IO[6]A) found in some tRNAs. Catalyzes the oxygen-dependent transformation of MS[2]I[6]A into MS[2]IO[6]A [H]

COG id: COG4445

COG function: function code FJ; Hydroxylase for synthesis of 2-methylthio-cis-ribozeatin in tRNA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012347
- InterPro:   IPR010386 [H]

Pfam domain/function: PF06175 MiaE [H]

EC number: NA

Molecular weight: Translated: 23284; Mature: 23284

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYPIPEIEAFLLCRTPDSWVQAALRNLDILLIDHANNEKKAAGAAFQFMFQYNDKFDLLS
CCCCCCCCEEEEECCCHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHH
KMSRLAREELRHFEQVISIIRKRQIPMANISSARYAGALRKLVRNHNPYRLTDALIVGAI
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
VEARSCERFAALVPHLDEELAKFYGGLLKSEARHFQDYLKLAYSYGDKADVDAKIEEIRL
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
AERELIESPDEEFRFHSGVPVAA
HHHHHHHCCCHHHHHCCCCCCCC
>Mature Secondary Structure
MYPIPEIEAFLLCRTPDSWVQAALRNLDILLIDHANNEKKAAGAAFQFMFQYNDKFDLLS
CCCCCCCCEEEEECCCHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHH
KMSRLAREELRHFEQVISIIRKRQIPMANISSARYAGALRKLVRNHNPYRLTDALIVGAI
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
VEARSCERFAALVPHLDEELAKFYGGLLKSEARHFQDYLKLAYSYGDKADVDAKIEEIRL
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
AERELIESPDEEFRFHSGVPVAA
HHHHHHHCCCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253666; 11677609 [H]