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The map label for this gene is folC [H]

Identifier: 66044910

GI number: 66044910

Start: 1869795

End: 1871099

Strand: Direct

Name: folC [H]

Synonym: Psyr_1665

Alternate gene names: 66044910

Gene position: 1869795-1871099 (Clockwise)

Preceding gene: 66044909

Following gene: 66044911

Centisome position: 30.68

GC content: 63.75

Gene sequence:

>1305_bases
ATGACTTCTACGTCCCTGAGCGACTGGCTCGCTTACCTGGAGCAACTGCATCCGTCTGCCATCGACATGGGGCTGGACCG
GTCTCGACAGGTGGCGCTGCAGCTGGGGCTTACCCGCCCGGCGCGCCGGGTGATCACGGTGACGGGCACCAACGGCAAGG
GTTCAACCTGCGCCTTTGTCGCCGCCTTGTTGCAGGCTCAGGGCCTGAAGGTCGGTGTCTACAGTTCCCCGCATCTGATT
CGCTATAACGAGCGGGTAAAGGTGCAGGGCGTCGAGGCAACCGACCTGGAGTTATGCGATGCCTTCGCTGCGGTCGAAGC
GGCACGCGGCGACGTCACCCTGACTTATTTCGAAATGGGCACGCTGGCGGCATTCTGGCTGTTCGAGCAGGCCCGGCTCG
ATGCGGTCGTTCTGGAAGTCGGTCTGGGCGGTCGCCTGGATGCGGTCAATCTCGTCGATGCGGATATGGCGCTGGTCACC
AGTATCGGGGTCGATCATGCCGAGTACCTGGGCGACACCCGTGAGTCGGTCGCCTTCGAGAAAGCCGGGATCTTTCGCGC
AGGTTGTCCTGCGCTGTGCGGTGATCCGGCGCCACCCGAGCCGCTGCTGGCCAAAGCCCGCGAGCTGGGCTGTCCGTTGT
TGTTGCGTGGCCGGGATTTCGATCTTTCCATAGGCGATGACAGCTGGGACTGGCGCGGTGTCGCTCACGGCGAACAGATC
GAGCTGCGCGGTCTGCCGCTGCTCGACCTGCCCATGCAGAATGCAGCGCTGGCGCTTCAGGCCTTCGCCCTGCTGGGGTA
TCCATTGCAACCGGATGCGACAGGCAAGGTACTTGCCGAGACCCGGCTCGCCGGTCGTCTCGATCGCAGGGTCGTCAAGT
GGCACGGCAAGTCGCTCAACCTGCTGCTGGATGTAGGCCATAACCCCCACGCGGCACAGTTTTTGGGGCAGCGCCTGGCG
CGGCAGCCGGTTGGCGGCAAGCGTCGTGCGGTGTTTGGCCTGCTCAGCGACAAGGATCTCGACGGTGTGGTCGCGCACCT
GGCGCCGGTCATTGAAGAGTGGGCCGTTGCGCCGCTTCCGACCTCGCGCAGCCGGCCGGCAGAGGAGTTGCAGACGGCCT
TGCAGAACCTTGGGGCCCGCGTGACGTCCTATCAGAGTATCGCGTTGGCGCTGGAAGCTCAGTGCGCGCATGCCACGCCC
GATGACGAGATTCTGCTGTTCGGTTCATTTTACTGTGTTGCCGAGGCCCTTGAATGGTTGGCCCGGTACACCGATGAGGA
CGCTGCAAATGGCTTTGCTGGATAA

Upstream 100 bases:

>100_bases
GCCTGGGTAACCTGCTGGCACAGATGATGAACCTGCCGACCCCTCGTTTTGTCGCCCCCGTTATCGAGCCAATCGTCGTC
CCGCCGGCCCCTGCGACGAT

Downstream 100 bases:

>100_bases
CGTATTCAAGCAGCGAATGGTGGGAGCACTGGTGCTGATCGCCGTGGCGGTCATCTTCCTGCCCATGCTTTTCACGCGTG
AAGATGAAACACGTCAGGTG

Product: folylpolyglutamate synthetase

Products: NA

Alternate protein names: Folylpolyglutamate synthase; Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase; Dihydrofolate synthase [H]

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MTSTSLSDWLAYLEQLHPSAIDMGLDRSRQVALQLGLTRPARRVITVTGTNGKGSTCAFVAALLQAQGLKVGVYSSPHLI
RYNERVKVQGVEATDLELCDAFAAVEAARGDVTLTYFEMGTLAAFWLFEQARLDAVVLEVGLGGRLDAVNLVDADMALVT
SIGVDHAEYLGDTRESVAFEKAGIFRAGCPALCGDPAPPEPLLAKARELGCPLLLRGRDFDLSIGDDSWDWRGVAHGEQI
ELRGLPLLDLPMQNAALALQAFALLGYPLQPDATGKVLAETRLAGRLDRRVVKWHGKSLNLLLDVGHNPHAAQFLGQRLA
RQPVGGKRRAVFGLLSDKDLDGVVAHLAPVIEEWAVAPLPTSRSRPAEELQTALQNLGARVTSYQSIALALEAQCAHATP
DDEILLFGSFYCVAEALEWLARYTDEDAANGFAG

Sequences:

>Translated_434_residues
MTSTSLSDWLAYLEQLHPSAIDMGLDRSRQVALQLGLTRPARRVITVTGTNGKGSTCAFVAALLQAQGLKVGVYSSPHLI
RYNERVKVQGVEATDLELCDAFAAVEAARGDVTLTYFEMGTLAAFWLFEQARLDAVVLEVGLGGRLDAVNLVDADMALVT
SIGVDHAEYLGDTRESVAFEKAGIFRAGCPALCGDPAPPEPLLAKARELGCPLLLRGRDFDLSIGDDSWDWRGVAHGEQI
ELRGLPLLDLPMQNAALALQAFALLGYPLQPDATGKVLAETRLAGRLDRRVVKWHGKSLNLLLDVGHNPHAAQFLGQRLA
RQPVGGKRRAVFGLLSDKDLDGVVAHLAPVIEEWAVAPLPTSRSRPAEELQTALQNLGARVTSYQSIALALEAQCAHATP
DDEILLFGSFYCVAEALEWLARYTDEDAANGFAG
>Mature_433_residues
TSTSLSDWLAYLEQLHPSAIDMGLDRSRQVALQLGLTRPARRVITVTGTNGKGSTCAFVAALLQAQGLKVGVYSSPHLIR
YNERVKVQGVEATDLELCDAFAAVEAARGDVTLTYFEMGTLAAFWLFEQARLDAVVLEVGLGGRLDAVNLVDADMALVTS
IGVDHAEYLGDTRESVAFEKAGIFRAGCPALCGDPAPPEPLLAKARELGCPLLLRGRDFDLSIGDDSWDWRGVAHGEQIE
LRGLPLLDLPMQNAALALQAFALLGYPLQPDATGKVLAETRLAGRLDRRVVKWHGKSLNLLLDVGHNPHAAQFLGQRLAR
QPVGGKRRAVFGLLSDKDLDGVVAHLAPVIEEWAVAPLPTSRSRPAEELQTALQNLGARVTSYQSIALALEAQCAHATPD
DEILLFGSFYCVAEALEWLARYTDEDAANGFAG

Specific function: Conversion of folates to polyglutamate derivatives [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=182, Percent_Identity=39.010989010989, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI66932990, Length=182, Percent_Identity=39.010989010989, Blast_Score=129, Evalue=4e-30,
Organism=Escherichia coli, GI1788654, Length=416, Percent_Identity=47.1153846153846, Blast_Score=344, Evalue=7e-96,
Organism=Caenorhabditis elegans, GI17553150, Length=374, Percent_Identity=29.144385026738, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI71984923, Length=385, Percent_Identity=28.5714285714286, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17553148, Length=385, Percent_Identity=28.5714285714286, Blast_Score=129, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6323760, Length=328, Percent_Identity=29.2682926829268, Blast_Score=118, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6324815, Length=179, Percent_Identity=35.195530726257, Blast_Score=112, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24641571, Length=151, Percent_Identity=38.4105960264901, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24581568, Length=146, Percent_Identity=34.9315068493151, Blast_Score=100, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17; =6.3.2.12 [H]

Molecular weight: Translated: 46770; Mature: 46639

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSTSLSDWLAYLEQLHPSAIDMGLDRSRQVALQLGLTRPARRVITVTGTNGKGSTCAFV
CCCCCHHHHHHHHHHHCCHHHHHCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCHHHHH
AALLQAQGLKVGVYSSPHLIRYNERVKVQGVEATDLELCDAFAAVEAARGDVTLTYFEMG
HHHHHHCCCEEEECCCCCEEEECCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEEHH
TLAAFWLFEQARLDAVVLEVGLGGRLDAVNLVDADMALVTSIGVDHAEYLGDTRESVAFE
HHHHHHHHHHHHHCEEEEEECCCCCCCEEEEECHHHHHHHHHCCCHHHHHCCCHHHHHHH
KAGIFRAGCPALCGDPAPPEPLLAKARELGCPLLLRGRDFDLSIGDDSWDWRGVAHGEQI
HCCCCCCCCCHHCCCCCCCCHHHHHHHHCCCCEEEECCCEEEEECCCCCCCCCCCCCCEE
ELRGLPLLDLPMQNAALALQAFALLGYPLQPDATGKVLAETRLAGRLDRRVVKWHGKSLN
EECCCCEECCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEE
LLLDVGHNPHAAQFLGQRLARQPVGGKRRAVFGLLSDKDLDGVVAHLAPVIEEWAVAPLP
EEEECCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCC
TSRSRPAEELQTALQNLGARVTSYQSIALALEAQCAHATPDDEILLFGSFYCVAEALEWL
CCCCCCHHHHHHHHHHHCCHHHHHHHEEEEEECHHCCCCCCCCEEEEEHHHHHHHHHHHH
ARYTDEDAANGFAG
HHHCCCCCCCCCCC
>Mature Secondary Structure 
TSTSLSDWLAYLEQLHPSAIDMGLDRSRQVALQLGLTRPARRVITVTGTNGKGSTCAFV
CCCCHHHHHHHHHHHCCHHHHHCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCHHHHH
AALLQAQGLKVGVYSSPHLIRYNERVKVQGVEATDLELCDAFAAVEAARGDVTLTYFEMG
HHHHHHCCCEEEECCCCCEEEECCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEEHH
TLAAFWLFEQARLDAVVLEVGLGGRLDAVNLVDADMALVTSIGVDHAEYLGDTRESVAFE
HHHHHHHHHHHHHCEEEEEECCCCCCCEEEEECHHHHHHHHHCCCHHHHHCCCHHHHHHH
KAGIFRAGCPALCGDPAPPEPLLAKARELGCPLLLRGRDFDLSIGDDSWDWRGVAHGEQI
HCCCCCCCCCHHCCCCCCCCHHHHHHHHCCCCEEEECCCEEEEECCCCCCCCCCCCCCEE
ELRGLPLLDLPMQNAALALQAFALLGYPLQPDATGKVLAETRLAGRLDRRVVKWHGKSLN
EECCCCEECCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEE
LLLDVGHNPHAAQFLGQRLARQPVGGKRRAVFGLLSDKDLDGVVAHLAPVIEEWAVAPLP
EEEECCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCC
TSRSRPAEELQTALQNLGARVTSYQSIALALEAQCAHATPDDEILLFGSFYCVAEALEWL
CCCCCCHHHHHHHHHHHCCHHHHHHHEEEEEECHHCCCCCCCCEEEEEHHHHHHHHHHHH
ARYTDEDAANGFAG
HHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3040739; 3040734; 9205837; 9278503; 1989505 [H]