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The map label for this gene is htrB [H]

Identifier: 66044859

GI number: 66044859

Start: 1816749

End: 1817681

Strand: Direct

Name: htrB [H]

Synonym: Psyr_1614

Alternate gene names: 66044859

Gene position: 1816749-1817681 (Clockwise)

Preceding gene: 66044853

Following gene: 66044860

Centisome position: 29.81

GC content: 59.16

Gene sequence:

>933_bases
ATGGATCGCCCGCGTCTTAAAGCCAGTTTCCTTCACCCCCGTTTCTGGCCGCTATGGCTGGGGCTGGGGCTCTTGTGGCT
GATTGTTCAATTGCCATTTTGTGTTCTGCTGGTCATCGGTCGGGCACTGGGCGCCATCATGTACCGTGTGGCCACTGATC
GAAAAAAAATCGCGTCGCGCAATCTGGAGCTGTGCTTCCCGCATTTGTCCGCGGCCGAGCGCAAGCGTCTGCTCAAGGAA
AACTTCGCCTCCACCGGGATTGCCTTTTTCGAGATGGCGATGAGCTGGTGGTGGTCGAAAAAACGTCTGGCCAGGCTTGC
CCACGTCGAAGGGCTCGAGCATTTGCAGAATGCCCAGGAAAAAGGCGAGGGCGTGATTCTGATGGCGCTGCATTTCACCA
CGCTGGAAATCGGCGCGGCGCTTTTGGGCCAGCAGCACACCATCGACGGCATGTATCGCGAACACAAGAGCCCGTTGTTC
GACTTCGTCCAGCGTCGCGGGCGCGAGCGCCATAACCTGGATTCGCTGGCTGTCGAGCGAGACGACGTGCGCGGCATGCT
CAAGTTACTGCGTGCCGGCCGGGCCATCTGGTATGCGCCGGATCAGGACTATGGCGCCAAGCAAAGCATTTTTGTGCCGC
TGTTCGGCATCCAGGCCGCGACCGTGACCGCCACCAGCAAGTTCGCCCGCCTGGGCCGTGCGCAGGTTGTGCCCTTCACT
CAGCGGCGTCTGGCAGACGGCAGTGGTTACCGGCTGGTGATCCATCCGCCGCTGGATAACTTTCCGGGGGAAAGCGACGA
AGCCGATTGCCTGCGCATCAACCAATGGGTGGAAAAGGCCGTCACTGAATGCCCGGAACAATATCTTTGGGCCCATCGAC
GCTTCAAAAGTCGACCGCCAGGTGAACCGAAGCTATACAACAAGCATAAATAA

Upstream 100 bases:

>100_bases
TGTCGCTAAAAGTGCCGAGACTTATAGCGAGAACGCCCGCGAGCCGCAAGCGGCCAGAACCGTTGTAGAATGCGCGGCCT
TTGTTCTGACGGAAGCCCAA

Downstream 100 bases:

>100_bases
AACGCGTCTTCGATCACCGAGGTCAGTTGTAGATGCCAGATCCTGCTGTTGCGATCAATGCTGTGAGGAGTCCGGTCGAA
CCGGTAACCGGGCTCATTCT

Product: lipid A biosynthesis lauroyl acyltransferase

Products: NA

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MDRPRLKASFLHPRFWPLWLGLGLLWLIVQLPFCVLLVIGRALGAIMYRVATDRKKIASRNLELCFPHLSAAERKRLLKE
NFASTGIAFFEMAMSWWWSKKRLARLAHVEGLEHLQNAQEKGEGVILMALHFTTLEIGAALLGQQHTIDGMYREHKSPLF
DFVQRRGRERHNLDSLAVERDDVRGMLKLLRAGRAIWYAPDQDYGAKQSIFVPLFGIQAATVTATSKFARLGRAQVVPFT
QRRLADGSGYRLVIHPPLDNFPGESDEADCLRINQWVEKAVTECPEQYLWAHRRFKSRPPGEPKLYNKHK

Sequences:

>Translated_310_residues
MDRPRLKASFLHPRFWPLWLGLGLLWLIVQLPFCVLLVIGRALGAIMYRVATDRKKIASRNLELCFPHLSAAERKRLLKE
NFASTGIAFFEMAMSWWWSKKRLARLAHVEGLEHLQNAQEKGEGVILMALHFTTLEIGAALLGQQHTIDGMYREHKSPLF
DFVQRRGRERHNLDSLAVERDDVRGMLKLLRAGRAIWYAPDQDYGAKQSIFVPLFGIQAATVTATSKFARLGRAQVVPFT
QRRLADGSGYRLVIHPPLDNFPGESDEADCLRINQWVEKAVTECPEQYLWAHRRFKSRPPGEPKLYNKHK
>Mature_310_residues
MDRPRLKASFLHPRFWPLWLGLGLLWLIVQLPFCVLLVIGRALGAIMYRVATDRKKIASRNLELCFPHLSAAERKRLLKE
NFASTGIAFFEMAMSWWWSKKRLARLAHVEGLEHLQNAQEKGEGVILMALHFTTLEIGAALLGQQHTIDGMYREHKSPLF
DFVQRRGRERHNLDSLAVERDDVRGMLKLLRAGRAIWYAPDQDYGAKQSIFVPLFGIQAATVTATSKFARLGRAQVVPFT
QRRLADGSGYRLVIHPPLDNFPGESDEADCLRINQWVEKAVTECPEQYLWAHRRFKSRPPGEPKLYNKHK

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=304, Percent_Identity=45.3947368421053, Blast_Score=293, Evalue=1e-80,
Organism=Escherichia coli, GI87082094, Length=306, Percent_Identity=43.7908496732026, Blast_Score=269, Evalue=2e-73,
Organism=Escherichia coli, GI1788161, Length=308, Percent_Identity=31.8181818181818, Blast_Score=149, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 35635; Mature: 35635

Theoretical pI: Translated: 10.43; Mature: 10.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRPRLKASFLHPRFWPLWLGLGLLWLIVQLPFCVLLVIGRALGAIMYRVATDRKKIASR
CCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NLELCFPHLSAAERKRLLKENFASTGIAFFEMAMSWWWSKKRLARLAHVEGLEHLQNAQE
CHHEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KGEGVILMALHFTTLEIGAALLGQQHTIDGMYREHKSPLFDFVQRRGRERHNLDSLAVER
HCCCEEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHCCHHCCHHHHHHHH
DDVRGMLKLLRAGRAIWYAPDQDYGAKQSIFVPLFGIQAATVTATSKFARLGRAQVVPFT
HHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCHH
QRRLADGSGYRLVIHPPLDNFPGESDEADCLRINQWVEKAVTECPEQYLWAHRRFKSRPP
HHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCC
GEPKLYNKHK
CCCCCCCCCC
>Mature Secondary Structure
MDRPRLKASFLHPRFWPLWLGLGLLWLIVQLPFCVLLVIGRALGAIMYRVATDRKKIASR
CCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NLELCFPHLSAAERKRLLKENFASTGIAFFEMAMSWWWSKKRLARLAHVEGLEHLQNAQE
CHHEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KGEGVILMALHFTTLEIGAALLGQQHTIDGMYREHKSPLFDFVQRRGRERHNLDSLAVER
HCCCEEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHCCHHCCHHHHHHHH
DDVRGMLKLLRAGRAIWYAPDQDYGAKQSIFVPLFGIQAATVTATSKFARLGRAQVVPFT
HHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCHH
QRRLADGSGYRLVIHPPLDNFPGESDEADCLRINQWVEKAVTECPEQYLWAHRRFKSRPP
HHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCC
GEPKLYNKHK
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]