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The map label for this gene is yafJ [H]

Identifier: 66044821

GI number: 66044821

Start: 1771329

End: 1772129

Strand: Direct

Name: yafJ [H]

Synonym: Psyr_1576

Alternate gene names: 66044821

Gene position: 1771329-1772129 (Clockwise)

Preceding gene: 66044820

Following gene: 66044822

Centisome position: 29.07

GC content: 59.68

Gene sequence:

>801_bases
ATGTGTGAACTACTGGGCATGAGCGCCAACGTCCCGACCGACATTGTCTTCAGCTTTACCGGGCTGATGCAGCGTGGCGG
CCGCACCGGTCCGCATCGCGATGGCTGGGGCATTGCCTTCTACGAGGGCCGTGGCCTGCGTCTGTTTCAGGACCCGGCAG
CCAGCAGCGAATCGGAAGTCGCACAGTTGGTGCAGCGTTATCCCATCAAGAGCGAAGTCGTGATTGGCCATATTCGGCAG
GCCAACGTCGGCAAGGTCTGCCTGTCCAATACCCATCCTTTCGTCCGTGAGCTCTGGGGCCGTAACTGGTGCTTTGCGCA
TAACGGTCAGCTGGCGGATTTCACCCCGGGCGTTACTTTTTATCGGCCAGTGGGCGATACCGACAGCGAAGCGGCATTCT
GCGACTTGCTCAATCGCGTCCGCGAAACCTTTCCCGAGCCCGTCGACGTCGAGGCGTTGCTGCCTACGCTGATTCAGGCG
TGTGCCGAATATCGCAGTAAAGGCGTGTTCAACTGCCTGTTGAGCGACGGCGACTGGCTGTTTTGCTTTTGCAGTACCAA
ACTGGTTCAGATCACTCGCCGTGCTCCGTTCGGTCCGGCGAGGTTGAAAGATGTCGACGTGATTGTCGATTTTCAGGCTG
AAACCACACCGCACGACGTGGTCACGGTCATCGCGACCGAACCCTTGACCGAGAACGAAAACTGGAACCGCTATGAGCCG
GGCCAGTGGAGCCTGTGGCGCAAGGGCGAGTGCGTGGTGCAAGGCTCGGTCGACACCGTCACCGAGACTGCCAGGCCGTA
A

Upstream 100 bases:

>100_bases
GCGCGGGTCGAGCCGCGGCTGTAGCTCCCGCTTCGTTGGCAAGCGCCGCTACCTGCGGCGCATGACCCGCTGCAAAACGT
CTTGAAGCGAGAACCTGAGC

Downstream 100 bases:

>100_bases
TACACCAGCACATCGGCTTACTGGATAAAAAAGAAGAGGGATATTGATGCTGCGAAGTTATCTGCGATTGGTCCTGTTCA
CCATGGGCCTGCTGTTCGGG

Product: glutamine amidotransferase, class-II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MCELLGMSANVPTDIVFSFTGLMQRGGRTGPHRDGWGIAFYEGRGLRLFQDPAASSESEVAQLVQRYPIKSEVVIGHIRQ
ANVGKVCLSNTHPFVRELWGRNWCFAHNGQLADFTPGVTFYRPVGDTDSEAAFCDLLNRVRETFPEPVDVEALLPTLIQA
CAEYRSKGVFNCLLSDGDWLFCFCSTKLVQITRRAPFGPARLKDVDVIVDFQAETTPHDVVTVIATEPLTENENWNRYEP
GQWSLWRKGECVVQGSVDTVTETARP

Sequences:

>Translated_266_residues
MCELLGMSANVPTDIVFSFTGLMQRGGRTGPHRDGWGIAFYEGRGLRLFQDPAASSESEVAQLVQRYPIKSEVVIGHIRQ
ANVGKVCLSNTHPFVRELWGRNWCFAHNGQLADFTPGVTFYRPVGDTDSEAAFCDLLNRVRETFPEPVDVEALLPTLIQA
CAEYRSKGVFNCLLSDGDWLFCFCSTKLVQITRRAPFGPARLKDVDVIVDFQAETTPHDVVTVIATEPLTENENWNRYEP
GQWSLWRKGECVVQGSVDTVTETARP
>Mature_266_residues
MCELLGMSANVPTDIVFSFTGLMQRGGRTGPHRDGWGIAFYEGRGLRLFQDPAASSESEVAQLVQRYPIKSEVVIGHIRQ
ANVGKVCLSNTHPFVRELWGRNWCFAHNGQLADFTPGVTFYRPVGDTDSEAAFCDLLNRVRETFPEPVDVEALLPTLIQA
CAEYRSKGVFNCLLSDGDWLFCFCSTKLVQITRRAPFGPARLKDVDVIVDFQAETTPHDVVTVIATEPLTENENWNRYEP
GQWSLWRKGECVVQGSVDTVTETARP

Specific function: Unknown

COG id: COG0121

COG function: function code R; Predicted glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786417, Length=255, Percent_Identity=61.1764705882353, Blast_Score=314, Evalue=4e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000583
- InterPro:   IPR017932 [H]

Pfam domain/function: PF00310 GATase_2 [H]

EC number: NA

Molecular weight: Translated: 29747; Mature: 29747

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCELLGMSANVPTDIVFSFTGLMQRGGRTGPHRDGWGIAFYEGRGLRLFQDPAASSESEV
CCCEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCEEEEECCCCCCHHHH
AQLVQRYPIKSEVVIGHIRQANVGKVCLSNTHPFVRELWGRNWCFAHNGQLADFTPGVTF
HHHHHHCCCCCCEEEEECCCCCCCEEEECCCCHHHHHHHCCCEEEEECCCCCCCCCCCEE
YRPVGDTDSEAAFCDLLNRVRETFPEPVDVEALLPTLIQACAEYRSKGVFNCLLSDGDWL
EECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCEE
FCFCSTKLVQITRRAPFGPARLKDVDVIVDFQAETTPHDVVTVIATEPLTENENWNRYEP
EEEECCHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCC
GQWSLWRKGECVVQGSVDTVTETARP
CCCCEEECCCEEEECCHHHHHHCCCC
>Mature Secondary Structure
MCELLGMSANVPTDIVFSFTGLMQRGGRTGPHRDGWGIAFYEGRGLRLFQDPAASSESEV
CCCEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCEEEEECCCCCCHHHH
AQLVQRYPIKSEVVIGHIRQANVGKVCLSNTHPFVRELWGRNWCFAHNGQLADFTPGVTF
HHHHHHCCCCCCEEEEECCCCCCCEEEECCCCHHHHHHHCCCEEEEECCCCCCCCCCCEE
YRPVGDTDSEAAFCDLLNRVRETFPEPVDVEALLPTLIQACAEYRSKGVFNCLLSDGDWL
EECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCEE
FCFCSTKLVQITRRAPFGPARLKDVDVIVDFQAETTPHDVVTVIATEPLTENENWNRYEP
EEEECCHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCC
GQWSLWRKGECVVQGSVDTVTETARP
CCCCEEECCCEEEECCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7596361; 9278503 [H]