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The map label for this gene is dnaB [H]

Identifier: 66044778

GI number: 66044778

Start: 1720372

End: 1721718

Strand: Reverse

Name: dnaB [H]

Synonym: Psyr_1531

Alternate gene names: 66044778

Gene position: 1721718-1720372 (Counterclockwise)

Preceding gene: 66044779

Following gene: 66044777

Centisome position: 28.25

GC content: 54.12

Gene sequence:

>1347_bases
ATGGACATTTCTCATATGTCACCTCCCCACTCCGTGGAAGCCGAACAAGCTGTGCTTGGCGGACTGATGCTTGATAACGA
TTCCTGGGACCTGATCGCAGACCGTATTAAAGCCGATGATTTCTACCGGGCCGAGCACCGTCTGATATTCCAGAGCATTG
CTGGGCTGGCTGAATCGAACAGGCCATTTGACGTGGTCACGGTTTCCGACTCCATTACAGCAATAGATGCTGCTGGGGGC
TTGGCTTATCTCAGTGAGCTCGCCAAAAACACGCCTTCTGTGGCCAACATCAAGGCTTATGCAGAAACAGTGAGTAACCG
AGCTCATCTACGCCGGATTATTTCACACGGCTATGCATGTACCCGGGAGGCTTCAGATCCTCAAGCGAATGCGTCTGAGG
TTCAGGACGTCATCGAGCAGCAACTTTTTGCTTTGGGGCAAGGACGGCAGACTTCCGACTTTGTGGACGTCAACCAGATG
CTTATGCAGGTGGTCGACAAGATCGATCTTCGCTTCAACGCTGGGGAGTCGGTCGTTGGATTGCCCACAGGGCTTTCCGA
TCTTGATGAATTGACGGGCGGACTACAACCAGCCGATCTGATCATCATTGCGGCCAGACCCTCCATGGGCAAAACCAGCC
TGGCGCTCAACTTGGTAGACGCTGCGCTTCAAAAAGATACTCAGGCCACTGTTCAGGTCTACAGCCTCGAGATGCCGGCA
GAGGCGATCATTTACAGGCTCATCTCGATCCTGGGGCACCTGAACCTGGAGAAGTTGATACGCGGGCAGTTGGAGGATGA
GGACTGGCCGAAGCTCGCGATGGCAGTGCAAAAGATCAACTCGTATGGCGATCGCCTTGTAATTGATGATCAAGCTGACC
TCACTCCCTCGGCAATTCGTGCGCGGGCTCGCAGAGGCGCACGGCGTTTCGGAAAGCCAGTCCTGATATTGATCGATTAT
CTGCAAATGATGAAATGTCCAGGTAAAGAAAATCGGACCAATGAAATCAGTGAGATATCTCGGTCACTCAAGGCTTTGGC
CAAAGAGTTCGACTGTCCTGTCGTGGCGCTCTCTCAGCTCAACAGAGAACTTGAGCGGCGTCCGAACAAGCGACCTATCA
ATGCAGACCTCCGTGAGTCGGGCGCGCTTGAGCAAGACGCTGACCTGATTCTGTTCGTATATCGGGACGAGGTTTACAAC
CCCGACAGCGAGCAAAAAGGGGTCGCCGAACTCATTCTCAGCAAGCATCGAAACGGCGCGCTCGGCACCGTCCGAACGGC
CTTCATCCCCCAACACACCCGTTTCGAAAACCTCTCGGCCGCCGCATGGCAAGGAGCTCGATCATGA

Upstream 100 bases:

>100_bases
TGAAATCTCAACATCAGGGCTGGACGCAAGGTTCTAGAGCAGCGTTGCACAGAGGCGTCGATCAGCACTCCCAGTCCCCG
GCAAGAATCGGGAGGGCTTG

Downstream 100 bases:

>100_bases
GCGCGCGCAATAAACCTCTTTGCATAACTCATGAGTGCCGCACGTGCGGCCACCACCACCCTTTCACCGCGGTCATGCCA
GCAGGAAAACGTGCGCCCGA

Product: DnaB helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MDISHMSPPHSVEAEQAVLGGLMLDNDSWDLIADRIKADDFYRAEHRLIFQSIAGLAESNRPFDVVTVSDSITAIDAAGG
LAYLSELAKNTPSVANIKAYAETVSNRAHLRRIISHGYACTREASDPQANASEVQDVIEQQLFALGQGRQTSDFVDVNQM
LMQVVDKIDLRFNAGESVVGLPTGLSDLDELTGGLQPADLIIIAARPSMGKTSLALNLVDAALQKDTQATVQVYSLEMPA
EAIIYRLISILGHLNLEKLIRGQLEDEDWPKLAMAVQKINSYGDRLVIDDQADLTPSAIRARARRGARRFGKPVLILIDY
LQMMKCPGKENRTNEISEISRSLKALAKEFDCPVVALSQLNRELERRPNKRPINADLRESGALEQDADLILFVYRDEVYN
PDSEQKGVAELILSKHRNGALGTVRTAFIPQHTRFENLSAAAWQGARS

Sequences:

>Translated_448_residues
MDISHMSPPHSVEAEQAVLGGLMLDNDSWDLIADRIKADDFYRAEHRLIFQSIAGLAESNRPFDVVTVSDSITAIDAAGG
LAYLSELAKNTPSVANIKAYAETVSNRAHLRRIISHGYACTREASDPQANASEVQDVIEQQLFALGQGRQTSDFVDVNQM
LMQVVDKIDLRFNAGESVVGLPTGLSDLDELTGGLQPADLIIIAARPSMGKTSLALNLVDAALQKDTQATVQVYSLEMPA
EAIIYRLISILGHLNLEKLIRGQLEDEDWPKLAMAVQKINSYGDRLVIDDQADLTPSAIRARARRGARRFGKPVLILIDY
LQMMKCPGKENRTNEISEISRSLKALAKEFDCPVVALSQLNRELERRPNKRPINADLRESGALEQDADLILFVYRDEVYN
PDSEQKGVAELILSKHRNGALGTVRTAFIPQHTRFENLSAAAWQGARS
>Mature_448_residues
MDISHMSPPHSVEAEQAVLGGLMLDNDSWDLIADRIKADDFYRAEHRLIFQSIAGLAESNRPFDVVTVSDSITAIDAAGG
LAYLSELAKNTPSVANIKAYAETVSNRAHLRRIISHGYACTREASDPQANASEVQDVIEQQLFALGQGRQTSDFVDVNQM
LMQVVDKIDLRFNAGESVVGLPTGLSDLDELTGGLQPADLIIIAARPSMGKTSLALNLVDAALQKDTQATVQVYSLEMPA
EAIIYRLISILGHLNLEKLIRGQLEDEDWPKLAMAVQKINSYGDRLVIDDQADLTPSAIRARARRGARRFGKPVLILIDY
LQMMKCPGKENRTNEISEISRSLKALAKEFDCPVVALSQLNRELERRPNKRPINADLRESGALEQDADLILFVYRDEVYN
PDSEQKGVAELILSKHRNGALGTVRTAFIPQHTRFENLSAAAWQGARS

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=449, Percent_Identity=50.1113585746102, Blast_Score=421, Evalue=1e-119,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 49413; Mature: 49413

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDISHMSPPHSVEAEQAVLGGLMLDNDSWDLIADRIKADDFYRAEHRLIFQSIAGLAESN
CCCCCCCCCCCCCHHHHHHCCEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
RPFDVVTVSDSITAIDAAGGLAYLSELAKNTPSVANIKAYAETVSNRAHLRRIISHGYAC
CCEEEEEECCCEEEHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEE
TREASDPQANASEVQDVIEQQLFALGQGRQTSDFVDVNQMLMQVVDKIDLRFNAGESVVG
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCEECCCCCEEE
LPTGLSDLDELTGGLQPADLIIIAARPSMGKTSLALNLVDAALQKDTQATVQVYSLEMPA
CCCCHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCHHEEEEEEECCCH
EAIIYRLISILGHLNLEKLIRGQLEDEDWPKLAMAVQKINSYGDRLVIDDQADLTPSAIR
HHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHH
ARARRGARRFGKPVLILIDYLQMMKCPGKENRTNEISEISRSLKALAKEFDCPVVALSQL
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHH
NRELERRPNKRPINADLRESGALEQDADLILFVYRDEVYNPDSEQKGVAELILSKHRNGA
HHHHHHCCCCCCCCCCHHHCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCC
LGTVRTAFIPQHTRFENLSAAAWQGARS
CHHHHHHHCCCCCCCCCCHHHHHCCCCC
>Mature Secondary Structure
MDISHMSPPHSVEAEQAVLGGLMLDNDSWDLIADRIKADDFYRAEHRLIFQSIAGLAESN
CCCCCCCCCCCCCHHHHHHCCEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
RPFDVVTVSDSITAIDAAGGLAYLSELAKNTPSVANIKAYAETVSNRAHLRRIISHGYAC
CCEEEEEECCCEEEHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEE
TREASDPQANASEVQDVIEQQLFALGQGRQTSDFVDVNQMLMQVVDKIDLRFNAGESVVG
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCEECCCCCEEE
LPTGLSDLDELTGGLQPADLIIIAARPSMGKTSLALNLVDAALQKDTQATVQVYSLEMPA
CCCCHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCHHEEEEEEECCCH
EAIIYRLISILGHLNLEKLIRGQLEDEDWPKLAMAVQKINSYGDRLVIDDQADLTPSAIR
HHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHH
ARARRGARRFGKPVLILIDYLQMMKCPGKENRTNEISEISRSLKALAKEFDCPVVALSQL
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHH
NRELERRPNKRPINADLRESGALEQDADLILFVYRDEVYNPDSEQKGVAELILSKHRNGA
HHHHHHCCCCCCCCCCHHHCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCC
LGTVRTAFIPQHTRFENLSAAAWQGARS
CHHHHHHHCCCCCCCCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]