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The map label for this gene is bfpB [H]

Identifier: 66044764

GI number: 66044764

Start: 1703673

End: 1705409

Strand: Reverse

Name: bfpB [H]

Synonym: Psyr_1517

Alternate gene names: 66044764

Gene position: 1705409-1703673 (Counterclockwise)

Preceding gene: 66044765

Following gene: 66044763

Centisome position: 27.99

GC content: 57.51

Gene sequence:

>1737_bases
ATGAGCATGCCCTTCATGCGTCAGACCGCTCTGAGTCTCGCTGTTCTAACGGCATCAGCATGCTCAGTGCAACGGGTGGA
TGAAGCCGCTGCACGGGCAGAGGCTACCGCCGATTCTGCCGGCCGTTACGCGGCTATCCAGCGTAACAAGCAGCAACAGG
AACGTCGGGACACAGTGATATTCAGCGACAAACCCTGGGTTTCAACGCAGCCAGTTGTTGCGAGACGAGGCATCCCCACC
AAGTATGACTGTGAGGTCGCATATAGGCCTGCTGGCAGTGTCGGAATTGCTGAAATTGCCCAGTACATTTCCAGACAGTG
CGGGATCCCTGTCCTGGTCTCGCCGGATGCGCTCAATCCTGGCCTGCTGAACGCTAATGCACCTGCACAGGGCAACAATG
CGCCTCCAATCAGCACGGCCCCCAACCCTGATAGCCTGGCCGGGTTGCTGCCTGCAGGTATCACGGGCGGCTCCAACCTG
GCGCAGGCCTCTCAGGGGAGAAGTTCTGACTTCGCATCCATGCTGACACCGAACCTCGTCAGTGGGTTGCGCTTCACAGG
GAAAGCCTCGGGTCTGCTGGATGAGGTCACCGCGCGGCTGGGACTCACCTATCGATTCAATCCCACCTCACGGTCAGTGC
AGGTCAGCTATTTCGACACCAAGGTGTTTGACGTCTACGCATTTGGAGACGTGCAGGAAATCAAAAGCACCGTCAGATCC
GGTATGACCACCTCCTCAGGTTCGGGAAGTGGTTCGTCGAGCGGATCATCGTCAGGTAGCAGTTCCTCAGGCGTTTCGGG
TGACTCGGGCAGCAACCAGAGCACTACAGTCACTCTCAACACATCGATCCTGACTGACATCCAGAGCAACGTGAGGGCAA
TGCTGAGTACCTCGCCGCCTGGTCGCATGTATCTGTCACCGTCGACCGGGACGTTGACCGTGACTGACCGGCCCGACGTG
CTTTCGAACGTTGAGACCTATCTGGCCAAGACCAACCACGCGATCACCCAACAAGTGCTGTTCAATGTGAAGGTGTTTGA
GGCGACGCTGACCGACACCGATCAGCTGGCTTTGAACTGGGCGGCCGTCTACAACTCGCTGAGCACAAAGTGGGGCCTGT
CTCTCTCGAATACTGTGCCAGGCATCAGCTCCAGCGCAATCTCGGGCTCTGTTGGCATTGTCGATACGGCCAACTCTGCC
TGGGCAGGTTCGAACGCGATCATCCAGGCAATCGCAGAGCAGGCCCGGATCTCGAATGTTCGATCGCCGTCTGTGACCAC
ATTGAATCTGCAGCCTGCTCCGCTGCAGATCGGCAACGTCCAAGGCTACATCCCATCGGTTCAGACCAACACCACGGCGT
CAGTGGGCTCCAGCACGGCAATTACTCCGGGCACTATCACCTCCGGCTTCAACATGACACTGCAGCCTCGCTTGATGGAC
GATGACGAGATGTTGCTCATGGTATCGATCAATATGAGCTCCAAACCTACCTTCGAGCCATTCACGAGCAACGGCTCCAG
CGTCCAGATCCCCAACTACGATGCGAAAAGTTTGTCACCGAAGGTGAAGCTGCGCAGCGGTCAGACATTGATCCTGTCGG
GCTTTGAGGAGCTCAGCGACAACACGGACAAGATCGGCACAGGTTCCCCCGGATTCTTCGGGCTTGGAGGTGGGCGTAAG
CGCACCTCGTCAAAAAGCGTTCTGGTAGTGCTGATCACCCCGATTGTGACCAACTGA

Upstream 100 bases:

>100_bases
GGCAGATATTCCCGCTGTACGACAACGCCAAACGGTCCTTTGTGGTGGACGGTAATGAGGGCAATGGTCAGCAGGTTCTG
CACGTATCGGAGAGGAAAAA

Downstream 100 bases:

>100_bases
CATGGCGAACCTGTTCTCTCTGATACGAAAGCGCGCTACTCGCCCCCCAACGCCCAGCGATGGCAAAGTTCAGATTCTGA
CTTATCACGGACGATCTTTC

Product: type II and III secretion system protein

Products: NA

Alternate protein names: Bundle-forming pilus B [H]

Number of amino acids: Translated: 578; Mature: 577

Protein sequence:

>578_residues
MSMPFMRQTALSLAVLTASACSVQRVDEAAARAEATADSAGRYAAIQRNKQQQERRDTVIFSDKPWVSTQPVVARRGIPT
KYDCEVAYRPAGSVGIAEIAQYISRQCGIPVLVSPDALNPGLLNANAPAQGNNAPPISTAPNPDSLAGLLPAGITGGSNL
AQASQGRSSDFASMLTPNLVSGLRFTGKASGLLDEVTARLGLTYRFNPTSRSVQVSYFDTKVFDVYAFGDVQEIKSTVRS
GMTTSSGSGSGSSSGSSSGSSSSGVSGDSGSNQSTTVTLNTSILTDIQSNVRAMLSTSPPGRMYLSPSTGTLTVTDRPDV
LSNVETYLAKTNHAITQQVLFNVKVFEATLTDTDQLALNWAAVYNSLSTKWGLSLSNTVPGISSSAISGSVGIVDTANSA
WAGSNAIIQAIAEQARISNVRSPSVTTLNLQPAPLQIGNVQGYIPSVQTNTTASVGSSTAITPGTITSGFNMTLQPRLMD
DDEMLLMVSINMSSKPTFEPFTSNGSSVQIPNYDAKSLSPKVKLRSGQTLILSGFEELSDNTDKIGTGSPGFFGLGGGRK
RTSSKSVLVVLITPIVTN

Sequences:

>Translated_578_residues
MSMPFMRQTALSLAVLTASACSVQRVDEAAARAEATADSAGRYAAIQRNKQQQERRDTVIFSDKPWVSTQPVVARRGIPT
KYDCEVAYRPAGSVGIAEIAQYISRQCGIPVLVSPDALNPGLLNANAPAQGNNAPPISTAPNPDSLAGLLPAGITGGSNL
AQASQGRSSDFASMLTPNLVSGLRFTGKASGLLDEVTARLGLTYRFNPTSRSVQVSYFDTKVFDVYAFGDVQEIKSTVRS
GMTTSSGSGSGSSSGSSSGSSSSGVSGDSGSNQSTTVTLNTSILTDIQSNVRAMLSTSPPGRMYLSPSTGTLTVTDRPDV
LSNVETYLAKTNHAITQQVLFNVKVFEATLTDTDQLALNWAAVYNSLSTKWGLSLSNTVPGISSSAISGSVGIVDTANSA
WAGSNAIIQAIAEQARISNVRSPSVTTLNLQPAPLQIGNVQGYIPSVQTNTTASVGSSTAITPGTITSGFNMTLQPRLMD
DDEMLLMVSINMSSKPTFEPFTSNGSSVQIPNYDAKSLSPKVKLRSGQTLILSGFEELSDNTDKIGTGSPGFFGLGGGRK
RTSSKSVLVVLITPIVTN
>Mature_577_residues
SMPFMRQTALSLAVLTASACSVQRVDEAAARAEATADSAGRYAAIQRNKQQQERRDTVIFSDKPWVSTQPVVARRGIPTK
YDCEVAYRPAGSVGIAEIAQYISRQCGIPVLVSPDALNPGLLNANAPAQGNNAPPISTAPNPDSLAGLLPAGITGGSNLA
QASQGRSSDFASMLTPNLVSGLRFTGKASGLLDEVTARLGLTYRFNPTSRSVQVSYFDTKVFDVYAFGDVQEIKSTVRSG
MTTSSGSGSGSSSGSSSGSSSSGVSGDSGSNQSTTVTLNTSILTDIQSNVRAMLSTSPPGRMYLSPSTGTLTVTDRPDVL
SNVETYLAKTNHAITQQVLFNVKVFEATLTDTDQLALNWAAVYNSLSTKWGLSLSNTVPGISSSAISGSVGIVDTANSAW
AGSNAIIQAIAEQARISNVRSPSVTTLNLQPAPLQIGNVQGYIPSVQTNTTASVGSSTAITPGTITSGFNMTLQPRLMDD
DEMLLMVSINMSSKPTFEPFTSNGSSVQIPNYDAKSLSPKVKLRSGQTLILSGFEELSDNTDKIGTGSPGFFGLGGGRKR
TSSKSVLVVLITPIVTN

Specific function: Is absolutely required for pilus biogenesis, and for EPEC localized adherence and autoaggregation. Acts at a step in the BFP biogenic pathway after production and processing of the structural pilus subunit BfpA [H]

COG id: COG1450

COG function: function code NU; Type II secretory pathway, component PulD

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011514
- InterPro:   IPR004846 [H]

Pfam domain/function: PF00263 Secretin; PF07655 Secretin_N_2 [H]

EC number: NA

Molecular weight: Translated: 60349; Mature: 60218

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMPFMRQTALSLAVLTASACSVQRVDEAAARAEATADSAGRYAAIQRNKQQQERRDTVI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHCCEEE
FSDKPWVSTQPVVARRGIPTKYDCEVAYRPAGSVGIAEIAQYISRQCGIPVLVSPDALNP
ECCCCCCCCCCHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCEEECCCCCCC
GLLNANAPAQGNNAPPISTAPNPDSLAGLLPAGITGGSNLAQASQGRSSDFASMLTPNLV
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHCCCCCHHHHHHCHHHH
SGLRFTGKASGLLDEVTARLGLTYRFNPTSRSVQVSYFDTKVFDVYAFGDVQEIKSTVRS
HCCEECCCCCCHHHHHHHHHCCEEEECCCCCEEEEEEECCEEEEEEECCCHHHHHHHHHC
GMTTSSGSGSGSSSGSSSGSSSSGVSGDSGSNQSTTVTLNTSILTDIQSNVRAMLSTSPP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHEEECCCCC
GRMYLSPSTGTLTVTDRPDVLSNVETYLAKTNHAITQQVLFNVKVFEATLTDTDQLALNW
CCEEECCCCCEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHEEEEEEEECCCCHHHHHHH
AAVYNSLSTKWGLSLSNTVPGISSSAISGSVGIVDTANSAWAGSNAIIQAIAEQARISNV
HHHHHCCCHHCCCEECCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCC
RSPSVTTLNLQPAPLQIGNVQGYIPSVQTNTTASVGSSTAITPGTITSGFNMTLQPRLMD
CCCCEEEEECCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCEECC
DDEMLLMVSINMSSKPTFEPFTSNGSSVQIPNYDAKSLSPKVKLRSGQTLILSGFEELSD
CCCEEEEEEECCCCCCCCCCCCCCCCEEECCCCCHHHCCCEEEECCCCEEEEECHHHHCC
NTDKIGTGSPGFFGLGGGRKRTSSKSVLVVLITPIVTN
CCCCCCCCCCCEEECCCCCCCCCCCCEEEEEEECCCCC
>Mature Secondary Structure 
SMPFMRQTALSLAVLTASACSVQRVDEAAARAEATADSAGRYAAIQRNKQQQERRDTVI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHCCEEE
FSDKPWVSTQPVVARRGIPTKYDCEVAYRPAGSVGIAEIAQYISRQCGIPVLVSPDALNP
ECCCCCCCCCCHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCEEECCCCCCC
GLLNANAPAQGNNAPPISTAPNPDSLAGLLPAGITGGSNLAQASQGRSSDFASMLTPNLV
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHCCCCCHHHHHHCHHHH
SGLRFTGKASGLLDEVTARLGLTYRFNPTSRSVQVSYFDTKVFDVYAFGDVQEIKSTVRS
HCCEECCCCCCHHHHHHHHHCCEEEECCCCCEEEEEEECCEEEEEEECCCHHHHHHHHHC
GMTTSSGSGSGSSSGSSSGSSSSGVSGDSGSNQSTTVTLNTSILTDIQSNVRAMLSTSPP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHEEECCCCC
GRMYLSPSTGTLTVTDRPDVLSNVETYLAKTNHAITQQVLFNVKVFEATLTDTDQLALNW
CCEEECCCCCEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHEEEEEEEECCCCHHHHHHH
AAVYNSLSTKWGLSLSNTVPGISSSAISGSVGIVDTANSAWAGSNAIIQAIAEQARISNV
HHHHHCCCHHCCCEECCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCC
RSPSVTTLNLQPAPLQIGNVQGYIPSVQTNTTASVGSSTAITPGTITSGFNMTLQPRLMD
CCCCEEEEECCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCEECC
DDEMLLMVSINMSSKPTFEPFTSNGSSVQIPNYDAKSLSPKVKLRSGQTLILSGFEELSD
CCCEEEEEEECCCCCCCCCCCCCCCCEEECCCCCHHHCCCEEEECCCCEEEEECHHHHCC
NTDKIGTGSPGFFGLGGGRKRTSSKSVLVVLITPIVTN
CCCCCCCCCCCEEECCCCCCCCCCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8626330; 10496929; 8932312 [H]