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The map label for this gene is yeiE [H]

Identifier: 66044725

GI number: 66044725

Start: 1667185

End: 1668228

Strand: Reverse

Name: yeiE [H]

Synonym: Psyr_1478

Alternate gene names: 66044725

Gene position: 1668228-1667185 (Counterclockwise)

Preceding gene: 66044727

Following gene: 66044724

Centisome position: 27.38

GC content: 53.54

Gene sequence:

>1044_bases
ATGAAGCTAAGCCTACGCCAGCTACAAATTTTCTGCGCCGTCGCGCAGCACGGTAGCACCACCAGCGCCGCCATTTCGGT
GTCACTGTCCCAATCTGCAACCAGCGCCGCACTGAACGAGTTGGAAAGCGCCTTGGATACCAGGCTGTTCGACCGTGTAG
GCAAGCGTTTAGTGCTGAACGATAACGGCCATGCCTTACTGCCTCAAGCCAGAAGGATGCTTGAGAATGCACAACAGATA
GAGGCCAGTTTCTTACCCGGCCATGCCGGGCTGAAGACACGCTTGCGGGTAGGATGCAGTACCACGATAGGCAATTATGT
TCTGCCATTGATTCTCGGTGAGCTACGTCAATCAACACCACATTTGAGGGTCGATGTCGACATGGCCAACAGCATGGCTA
TCGCAAGAAGAGTCGCTGATTTCGAGATTGATATGGGCCTGATCGAGGGGCCTTGCCACCTGCCCGAACTCAGTGCCGAG
CCTTGGCTAATTGATGAGTTGCTTATTGCCGCAGGCCAACGGCACCCCTTGGCACTAAAGCAGCAGGTCACACTTGATGA
TCTTCGTACCGCTGAATGGCTACTGCGCGAGCCAGGTTCTGGTACCCGTGAAGAAGTCGAACAATTGCTGCTGCGTCATT
TGCACGATCTCGCCGAAACGCGCCAGATCGGCAGTTCAGAGGCCATCAAGCATACCCTCGCACAAGGCATCGGCATCAGT
TGCCTGTCCCGCTGGGTCGTTCGGGATCTACTGGCCTCTGAAGTCTTGGTTGAACTGCCGCACGCGTTGCCTGCACTGAC
CCGACGTTTTTTTCTGATCCGTCACCGGGACAAATACATCTCGCCTGGGCTTGAGCGATTCTGGGAAAAATGCAGTCAAT
TCGTGAGCCAGGAGCAGCTAGACCTATTTATAAAACAGGTAAGAGCTACCAAAACAAGTTGTTTCCGGAATTTATCAACG
ACGCATAAAGTCAACTCATTGCCCTGCAGCGATGAACGAGTTGTATATGCCAAGGCGCGACTGGGTGCCTTGCTTACCCT
ATGA

Upstream 100 bases:

>100_bases
AGTGTGCCTTCGATATGACATGGTTGAAGTATAGCTTGATGGGAATAGCGGTAAAAACGGATAGTATAGGTTTATATGAC
CTATAAAGAAGGTAGATTAC

Downstream 100 bases:

>100_bases
GGAAATTCTGATGAATGTGATAAAGAAAACCGTCACAGCCCTGACGTTGGGTGTTGCGTTGGCCGCATCGATGAGCGCGT
GGGCCGCGCAAGATCGCTGG

Product: regulatory protein, LysR:LysR, substrate-binding

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MKLSLRQLQIFCAVAQHGSTTSAAISVSLSQSATSAALNELESALDTRLFDRVGKRLVLNDNGHALLPQARRMLENAQQI
EASFLPGHAGLKTRLRVGCSTTIGNYVLPLILGELRQSTPHLRVDVDMANSMAIARRVADFEIDMGLIEGPCHLPELSAE
PWLIDELLIAAGQRHPLALKQQVTLDDLRTAEWLLREPGSGTREEVEQLLLRHLHDLAETRQIGSSEAIKHTLAQGIGIS
CLSRWVVRDLLASEVLVELPHALPALTRRFFLIRHRDKYISPGLERFWEKCSQFVSQEQLDLFIKQVRATKTSCFRNLST
THKVNSLPCSDERVVYAKARLGALLTL

Sequences:

>Translated_347_residues
MKLSLRQLQIFCAVAQHGSTTSAAISVSLSQSATSAALNELESALDTRLFDRVGKRLVLNDNGHALLPQARRMLENAQQI
EASFLPGHAGLKTRLRVGCSTTIGNYVLPLILGELRQSTPHLRVDVDMANSMAIARRVADFEIDMGLIEGPCHLPELSAE
PWLIDELLIAAGQRHPLALKQQVTLDDLRTAEWLLREPGSGTREEVEQLLLRHLHDLAETRQIGSSEAIKHTLAQGIGIS
CLSRWVVRDLLASEVLVELPHALPALTRRFFLIRHRDKYISPGLERFWEKCSQFVSQEQLDLFIKQVRATKTSCFRNLST
THKVNSLPCSDERVVYAKARLGALLTL
>Mature_347_residues
MKLSLRQLQIFCAVAQHGSTTSAAISVSLSQSATSAALNELESALDTRLFDRVGKRLVLNDNGHALLPQARRMLENAQQI
EASFLPGHAGLKTRLRVGCSTTIGNYVLPLILGELRQSTPHLRVDVDMANSMAIARRVADFEIDMGLIEGPCHLPELSAE
PWLIDELLIAAGQRHPLALKQQVTLDDLRTAEWLLREPGSGTREEVEQLLLRHLHDLAETRQIGSSEAIKHTLAQGIGIS
CLSRWVVRDLLASEVLVELPHALPALTRRFFLIRHRDKYISPGLERFWEKCSQFVSQEQLDLFIKQVRATKTSCFRNLST
THKVNSLPCSDERVVYAKARLGALLTL

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788481, Length=291, Percent_Identity=46.7353951890034, Blast_Score=259, Evalue=1e-70,
Organism=Escherichia coli, GI1788748, Length=292, Percent_Identity=26.3698630136986, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI157672245, Length=203, Percent_Identity=29.5566502463054, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI145693105, Length=291, Percent_Identity=25.085910652921, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI87082132, Length=299, Percent_Identity=23.7458193979933, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI87081904, Length=269, Percent_Identity=28.996282527881, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1790399, Length=272, Percent_Identity=25.3676470588235, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787601, Length=289, Percent_Identity=23.1833910034602, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787879, Length=251, Percent_Identity=25.8964143426295, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788296, Length=249, Percent_Identity=27.3092369477912, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1786401, Length=303, Percent_Identity=24.4224422442244, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789204, Length=257, Percent_Identity=24.5136186770428, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 38682; Mature: 38682

Theoretical pI: Translated: 8.10; Mature: 8.10

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSLRQLQIFCAVAQHGSTTSAAISVSLSQSATSAALNELESALDTRLFDRVGKRLVLN
CCCHHHHHHHHHHHHHCCCCCHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEC
DNGHALLPQARRMLENAQQIEASFLPGHAGLKTRLRVGCSTTIGNYVLPLILGELRQSTP
CCCCEECHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
HLRVDVDMANSMAIARRVADFEIDMGLIEGPCHLPELSAEPWLIDELLIAAGQRHPLALK
CEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHH
QQVTLDDLRTAEWLLREPGSGTREEVEQLLLRHLHDLAETRQIGSSEAIKHTLAQGIGIS
HHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHH
CLSRWVVRDLLASEVLVELPHALPALTRRFFLIRHRDKYISPGLERFWEKCSQFVSQEQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHH
DLFIKQVRATKTSCFRNLSTTHKVNSLPCSDERVVYAKARLGALLTL
HHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCEEEEHHHHHHHHCC
>Mature Secondary Structure
MKLSLRQLQIFCAVAQHGSTTSAAISVSLSQSATSAALNELESALDTRLFDRVGKRLVLN
CCCHHHHHHHHHHHHHCCCCCHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEC
DNGHALLPQARRMLENAQQIEASFLPGHAGLKTRLRVGCSTTIGNYVLPLILGELRQSTP
CCCCEECHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
HLRVDVDMANSMAIARRVADFEIDMGLIEGPCHLPELSAEPWLIDELLIAAGQRHPLALK
CEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHH
QQVTLDDLRTAEWLLREPGSGTREEVEQLLLRHLHDLAETRQIGSSEAIKHTLAQGIGIS
HHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHH
CLSRWVVRDLLASEVLVELPHALPALTRRFFLIRHRDKYISPGLERFWEKCSQFVSQEQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHH
DLFIKQVRATKTSCFRNLSTTHKVNSLPCSDERVVYAKARLGALLTL
HHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCEEEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]