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The map label for this gene is topB [H]

Identifier: 66044720

GI number: 66044720

Start: 1659653

End: 1661674

Strand: Reverse

Name: topB [H]

Synonym: Psyr_1473

Alternate gene names: 66044720

Gene position: 1661674-1659653 (Counterclockwise)

Preceding gene: 66044721

Following gene: 66044719

Centisome position: 27.27

GC content: 55.79

Gene sequence:

>2022_bases
ATGCGTTTGTATCTGTGCGAGAAGCCCAGCCAGGGTAAAGACATCGCCGCTGTACTCGGAGCCAAAACACGAGGTGACGG
CTGCATCAAAGGCAACGGTGTAGCCGTGACCTGGGGAATTGGACACTTGCTGGAAACAGCCCCGCCCGATGCATACGGCG
AGCACCTGAAAAACTGGAGCTTGGACACGCTTCCAATCCTGCCTGCGGAATGGAAAGTGCTCGTCAAGCCGAAAACCGCC
AGTCAGTTCAAGATCGTCAAGCAGCTTCTCAAGCAGGCTACCGAGCTCGTCATTGCGACAGACGCTGACCGCGAGGGGGA
GATGATCGCGCGCGAACTGATCGAGTATTGTGGGTACCGAGGACCGATTCAGAGGCTATGGCTGTCTGCGCTCAATGAAG
CATCCATCCGCCAAGCGCTCAACACCGTCAAGCAAGGCTCAGAAACCTACCCGTTGTACCTCTCGGCGCTCGCTCGCTCC
AGAGCGGACTGGCTCATCGGTATGAATTTTTCGCGGCTATTCACCCTGCTCGGCCGACAGGCCGGGTACACCGGTGTGCT
CTCCGTCGGCCGTGTCCAAACACCGACCCTTCGCCTGGTGGTCGACCGCGACCGGGATATCTCGAACTTCATACCCAAAC
CGTTCTGGAGTGTCGAGGTTCAGCTCTGGACGGCAGGACAATCGTTCTTGGCGAAATGGGTCGCCGATGAATACCTGGTA
GATGAGGAAGGCCGTTGCCTTGATCAAGCTGCTGCAGCTGCTGCACTGGCCGCGCTGAAAAGTAGTCAGGCTGCCTCAAC
AGTTTCTGTTGATACAAAGCGTGGCAAGGATCCAGCTCCGCTCCCGTTCGATCTGAGCACCCTGCAGGAGGTGTGCTCTG
CAAAGTTTGGGATGGGTGTGCAAGAGACGCTGGACGTAGCACAGTCACTTTACGAAACTCACAAGGCGACGACCTACCCA
AGGACCGATTGCGGGTACCTGCCGGAGAGCATGTTTGACGAAGTGCCGATGGTCCTCGATGCTCTGAATCGAACTGATCC
ATCCATCGGCAAGACGCTGCAACTGATCGACCCGGAGCAACGTTCCCGGGCATGGAATGACAAAAAAATCACTGGTCCCC
ACCATGGGATAATCCCCACGCTTGAACCTGCAAACCTTTCAGCAATGTCTGAGAAGGAACGCAAGGTCTATGAATTGATC
CGCGCCCACTTCCTTGCGCAGTTCCTGCCTACCCATGAGTACGACCGGACCGTGGCCACCTTCGAATGCAATGCAGTTCT
GCTGCAAGCAGTGGGCAAGCGAATCGTGATCCCAGGCTGGAAGGTTCTTTTTTCTGCATCGTCCGAAGAAGAGGCTAATG
ACAGTGGCGGCCGCTCTCAGATCCTGCCAGTGCTGCAAATTGGCACTCGGTGTGACGTTCAGGACCTGCATCTCAAATCG
CTGAAAACCGAGCCGCCCAAGGCTTACACCGAAGGTACATTGATCAAAGCGATGAAAACCATTGCCAAGCTGGTGAAGGA
TCCACGACTGGCTCAAAAGCTCAAGGAAACCACCGGTATTGGTACTGAAGCAACTCGCGCAGGCACGATTCAGGGCTTGA
TAGACCGAGGGTCACTCATCAAGAAAGGTAGCGCGCTGCGAGCAACTGAAGCAGCATTCTCGCTAATTGATGCGGTCCCT
CCTGCTGTGGCCGATCCCGGCACAACTGCGATATGGGAGCAAGCGCTTACGATGATCGAGCAAGGCACCTTGACGCTCGA
TGATTTTATCGCCAGGCAATCGAGCTGGATCACCAGGCTCGTCGAACAATACAAAGGCACCACCTTGTCGATCAAAGTGC
CCGAAGGTCCCAAGTGCCCTTTGTGCAGTGCGAAAACCTCGCAGCGTAAAGGATCCTCAGGCGTTTTCTGGGCGTGTTCC
CGCTACCCCGAATGCAAAGGTACGGTGAACATTGGTACAGGGAAGAAAAAATCACCTGGCAAGTCAGCCAAGCCCCGAGC
CGCTACTGTCAAGAGCAGCTGA

Upstream 100 bases:

>100_bases
CATAAACCGATTGTGACTTGGTTTAGGGTTTCATGAGACGGCGTTCAAGATTTAAGTGCATCGTCTCTTTCCTAAGCTGC
AACAACTCATCGGAGTCCTT

Downstream 100 bases:

>100_bases
GAGGCTGGTGATAGCGGGGTTACAAATGATGTGCCACAATGGGGCTACTACCAGTTCCACCCCGCTGGTCACTGACGGTC
ATCAGTGACACCCTGGAGAA

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 673; Mature: 673

Protein sequence:

>673_residues
MRLYLCEKPSQGKDIAAVLGAKTRGDGCIKGNGVAVTWGIGHLLETAPPDAYGEHLKNWSLDTLPILPAEWKVLVKPKTA
SQFKIVKQLLKQATELVIATDADREGEMIARELIEYCGYRGPIQRLWLSALNEASIRQALNTVKQGSETYPLYLSALARS
RADWLIGMNFSRLFTLLGRQAGYTGVLSVGRVQTPTLRLVVDRDRDISNFIPKPFWSVEVQLWTAGQSFLAKWVADEYLV
DEEGRCLDQAAAAAALAALKSSQAASTVSVDTKRGKDPAPLPFDLSTLQEVCSAKFGMGVQETLDVAQSLYETHKATTYP
RTDCGYLPESMFDEVPMVLDALNRTDPSIGKTLQLIDPEQRSRAWNDKKITGPHHGIIPTLEPANLSAMSEKERKVYELI
RAHFLAQFLPTHEYDRTVATFECNAVLLQAVGKRIVIPGWKVLFSASSEEEANDSGGRSQILPVLQIGTRCDVQDLHLKS
LKTEPPKAYTEGTLIKAMKTIAKLVKDPRLAQKLKETTGIGTEATRAGTIQGLIDRGSLIKKGSALRATEAAFSLIDAVP
PAVADPGTTAIWEQALTMIEQGTLTLDDFIARQSSWITRLVEQYKGTTLSIKVPEGPKCPLCSAKTSQRKGSSGVFWACS
RYPECKGTVNIGTGKKKSPGKSAKPRAATVKSS

Sequences:

>Translated_673_residues
MRLYLCEKPSQGKDIAAVLGAKTRGDGCIKGNGVAVTWGIGHLLETAPPDAYGEHLKNWSLDTLPILPAEWKVLVKPKTA
SQFKIVKQLLKQATELVIATDADREGEMIARELIEYCGYRGPIQRLWLSALNEASIRQALNTVKQGSETYPLYLSALARS
RADWLIGMNFSRLFTLLGRQAGYTGVLSVGRVQTPTLRLVVDRDRDISNFIPKPFWSVEVQLWTAGQSFLAKWVADEYLV
DEEGRCLDQAAAAAALAALKSSQAASTVSVDTKRGKDPAPLPFDLSTLQEVCSAKFGMGVQETLDVAQSLYETHKATTYP
RTDCGYLPESMFDEVPMVLDALNRTDPSIGKTLQLIDPEQRSRAWNDKKITGPHHGIIPTLEPANLSAMSEKERKVYELI
RAHFLAQFLPTHEYDRTVATFECNAVLLQAVGKRIVIPGWKVLFSASSEEEANDSGGRSQILPVLQIGTRCDVQDLHLKS
LKTEPPKAYTEGTLIKAMKTIAKLVKDPRLAQKLKETTGIGTEATRAGTIQGLIDRGSLIKKGSALRATEAAFSLIDAVP
PAVADPGTTAIWEQALTMIEQGTLTLDDFIARQSSWITRLVEQYKGTTLSIKVPEGPKCPLCSAKTSQRKGSSGVFWACS
RYPECKGTVNIGTGKKKSPGKSAKPRAATVKSS
>Mature_673_residues
MRLYLCEKPSQGKDIAAVLGAKTRGDGCIKGNGVAVTWGIGHLLETAPPDAYGEHLKNWSLDTLPILPAEWKVLVKPKTA
SQFKIVKQLLKQATELVIATDADREGEMIARELIEYCGYRGPIQRLWLSALNEASIRQALNTVKQGSETYPLYLSALARS
RADWLIGMNFSRLFTLLGRQAGYTGVLSVGRVQTPTLRLVVDRDRDISNFIPKPFWSVEVQLWTAGQSFLAKWVADEYLV
DEEGRCLDQAAAAAALAALKSSQAASTVSVDTKRGKDPAPLPFDLSTLQEVCSAKFGMGVQETLDVAQSLYETHKATTYP
RTDCGYLPESMFDEVPMVLDALNRTDPSIGKTLQLIDPEQRSRAWNDKKITGPHHGIIPTLEPANLSAMSEKERKVYELI
RAHFLAQFLPTHEYDRTVATFECNAVLLQAVGKRIVIPGWKVLFSASSEEEANDSGGRSQILPVLQIGTRCDVQDLHLKS
LKTEPPKAYTEGTLIKAMKTIAKLVKDPRLAQKLKETTGIGTEATRAGTIQGLIDRGSLIKKGSALRATEAAFSLIDAVP
PAVADPGTTAIWEQALTMIEQGTLTLDDFIARQSSWITRLVEQYKGTTLSIKVPEGPKCPLCSAKTSQRKGSSGVFWACS
RYPECKGTVNIGTGKKKSPGKSAKPRAATVKSS

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=506, Percent_Identity=25.4940711462451, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI10835218, Length=706, Percent_Identity=25.2124645892351, Blast_Score=115, Evalue=1e-25,
Organism=Escherichia coli, GI1788061, Length=617, Percent_Identity=40.5186385737439, Blast_Score=446, Evalue=1e-126,
Organism=Escherichia coli, GI1787529, Length=613, Percent_Identity=27.4061990212072, Blast_Score=160, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI32563869, Length=621, Percent_Identity=25.9259259259259, Blast_Score=134, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17555378, Length=622, Percent_Identity=25.5627009646302, Blast_Score=128, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6323263, Length=576, Percent_Identity=25.5208333333333, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24640096, Length=648, Percent_Identity=25, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24585251, Length=713, Percent_Identity=24.5441795231417, Blast_Score=115, Evalue=7e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 73558; Mature: 73558

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLYLCEKPSQGKDIAAVLGAKTRGDGCIKGNGVAVTWGIGHLLETAPPDAYGEHLKNWS
CEEEEECCCCCCCCHHHHHCCCCCCCCCEECCCEEEEECHHHHHHCCCCCHHHHHHCCCC
LDTLPILPAEWKVLVKPKTASQFKIVKQLLKQATELVIATDADREGEMIARELIEYCGYR
CCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHCCCC
GPIQRLWLSALNEASIRQALNTVKQGSETYPLYLSALARSRADWLIGMNFSRLFTLLGRQ
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEECCCHHHHHHHHHHC
AGYTGVLSVGRVQTPTLRLVVDRDRDISNFIPKPFWSVEVQLWTAGQSFLAKWVADEYLV
CCCHHHEECCCCCCCEEEEEEECCCCHHHCCCCCCEEEEEEEEECCHHHHHHHHHHHHHC
DEEGRCLDQAAAAAALAALKSSQAASTVSVDTKRGKDPAPLPFDLSTLQEVCSAKFGMGV
CCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCH
QETLDVAQSLYETHKATTYPRTDCGYLPESMFDEVPMVLDALNRTDPSIGKTLQLIDPEQ
HHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHH
RSRAWNDKKITGPHHGIIPTLEPANLSAMSEKERKVYELIRAHFLAQFLPTHEYDRTVAT
HHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
FECNAVLLQAVGKRIVIPGWKVLFSASSEEEANDSGGRSQILPVLQIGTRCDVQDLHLKS
EEHHHHHHHHCCCEEECCCCEEEEECCCCCCCCCCCCHHHCCHHHHCCCCCCHHHHHHHH
LKTEPPKAYTEGTLIKAMKTIAKLVKDPRLAQKLKETTGIGTEATRAGTIQGLIDRGSLI
CCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCHH
KKGSALRATEAAFSLIDAVPPAVADPGTTAIWEQALTMIEQGTLTLDDFIARQSSWITRL
HCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
VEQYKGTTLSIKVPEGPKCPLCSAKTSQRKGSSGVFWACSRYPECKGTVNIGTGKKKSPG
HHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEECCCCCCCCC
KSAKPRAATVKSS
CCCCCCCCCCCCC
>Mature Secondary Structure
MRLYLCEKPSQGKDIAAVLGAKTRGDGCIKGNGVAVTWGIGHLLETAPPDAYGEHLKNWS
CEEEEECCCCCCCCHHHHHCCCCCCCCCEECCCEEEEECHHHHHHCCCCCHHHHHHCCCC
LDTLPILPAEWKVLVKPKTASQFKIVKQLLKQATELVIATDADREGEMIARELIEYCGYR
CCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHCCCC
GPIQRLWLSALNEASIRQALNTVKQGSETYPLYLSALARSRADWLIGMNFSRLFTLLGRQ
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEECCCHHHHHHHHHHC
AGYTGVLSVGRVQTPTLRLVVDRDRDISNFIPKPFWSVEVQLWTAGQSFLAKWVADEYLV
CCCHHHEECCCCCCCEEEEEEECCCCHHHCCCCCCEEEEEEEEECCHHHHHHHHHHHHHC
DEEGRCLDQAAAAAALAALKSSQAASTVSVDTKRGKDPAPLPFDLSTLQEVCSAKFGMGV
CCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCH
QETLDVAQSLYETHKATTYPRTDCGYLPESMFDEVPMVLDALNRTDPSIGKTLQLIDPEQ
HHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHH
RSRAWNDKKITGPHHGIIPTLEPANLSAMSEKERKVYELIRAHFLAQFLPTHEYDRTVAT
HHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
FECNAVLLQAVGKRIVIPGWKVLFSASSEEEANDSGGRSQILPVLQIGTRCDVQDLHLKS
EEHHHHHHHHCCCEEECCCCEEEEECCCCCCCCCCCCHHHCCHHHHCCCCCCHHHHHHHH
LKTEPPKAYTEGTLIKAMKTIAKLVKDPRLAQKLKETTGIGTEATRAGTIQGLIDRGSLI
CCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCHH
KKGSALRATEAAFSLIDAVPPAVADPGTTAIWEQALTMIEQGTLTLDDFIARQSSWITRL
HCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
VEQYKGTTLSIKVPEGPKCPLCSAKTSQRKGSSGVFWACSRYPECKGTVNIGTGKKKSPG
HHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEECCCCCCCCC
KSAKPRAATVKSS
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2553698; 9097039; 9278503; 10574789 [H]