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The map label for this gene is proS [H]

Identifier: 66044647

GI number: 66044647

Start: 1584734

End: 1586449

Strand: Reverse

Name: proS [H]

Synonym: Psyr_1399

Alternate gene names: 66044647

Gene position: 1586449-1584734 (Counterclockwise)

Preceding gene: 66044648

Following gene: 66044646

Centisome position: 26.03

GC content: 60.02

Gene sequence:

>1716_bases
ATGCGCACCAGTCAATTTTTGCTCGCCACACAGAAAGAAACCCCTTCCGATGCGGTCGTGGTCAGCCATCAGCTGATGCT
GCGCGCCGGCATGATTCGCAAGCTGGCCTCCGGCCTCTACACCTGGCTGCCGATGGGCCTGCGCGTACTGCGCAAGGTCG
AAGCCATCGTTCGTGAAGAAATGGACGCCGCAGGCGCACTCGAAATTCTGATGCCCGGCATTCAACCCGCAGAACTGTGG
CAGGAATCCGGACGCTGGGAGCAGTATGGCCCGGAGCTGATGCGTCTGGTCGACCGCCACAACCGTGAGTTCTGCCTGGG
CCCGACCCACGAAGAAGTCATCACCGATCTGGCACGCAATGAGCTCAACAGCTATAAACAGCTGCCGATCAACATGTACC
AGATCCAGACCAAATTCCGTGACGAGATCCGCCCACGCTTCGGCCTGATGCGTGGACGCGAGTTCGTCATGAAAGACGCC
TACTCGTTCCATGCCGATCACGAATCGTTGCAGGTCACCTACGACCGCATGCATCTGGCGTACAGCAACATCTTCACCCG
ACTGGGCCTGAAGTTTCGCCCGGTCGAAGCGGATAACGGCTCGATTGGTGGCGCAGGCTCGCACGAATTCCACGTACTGG
CCGAATCCGGCGAAGACGATATCGTGTTCAGCGACGGCTCCGATTACGCAGCCAACATCGAAAAGGCCGAGGCGATTCCT
CGCGAGAAGACGCGCCCTGCCGCGACCGAAGAGCTGCGTCTGATCGACACGCCGAACACCAAGACCATCGCCCAACTGGT
CGAAGGTTTCGGTCTGCCAATCGAGAAAACCGTCAAGACCCTGGTGGTGCATGCTGCCGAAGAAGGCAAGCTGATCGCCC
TGATCATCCGCGGCGATCACGAGCTCAATGAAATCAAGGCCAGTCAGCTGGAACAGGTCGCCAATCCGCTGGTCATGGCG
TCCGAAGCCGAACTGCGCGACGCCATCGGCGCAGGCGCGGGCTCACTGGGCCCATTGAACCTGCCACTGCCGTGCATCAT
CGACCGCTCTGTCGAGCTGATGAGCGACTTCAGTGTCGGTGCCAACATCGACGACAAACACTATTTCGGCGTCAACTGGG
AGCGCGATCTGCCGGTTCCGACCGTTGCCGACCTGCGCAACGTGGTTGCGGGCGACCCAAGCCCGGACGGCAAGGGCACG
CTGGAAATCAAGCGCGGCATCGAAGTGGGTCACATCTTCCAGCTGGGCACCAAGTACAGCGAAGCGATGAAGTGCCAGGT
GCTGGGTGAAAACGGCAAGCCGGTCAATCTGGCGATGGGTTGCTACGGCATCGGTGTTTCCCGAGTGGTTGCCGCTGCCA
TCGAACAGAACAGCGACGAAAACGGCATCATCTGGAACGACACCCTCGCGCCGTTCCAGATTGCGCTGGTCCCGCTGCGC
TACGAGACGGACGCCGTTCGTGAAGCGACGGACAAACTCTACGCCGACCTGACGGCTGCCGGTTTTGAAGTACTGCTCGA
CGACCGCGACAAGAAGACCAGCCCGGGCATCAAGTTCGCCGACATGGAACTGATCGGTATCCCGCACCGTATCGTGGTCA
GTGACCGAGGCCTGGCCGAAGGTAATCTGGAATACAAGAGCCGTACCGAATCGCAACCGCAAGCCATTGCGGTCGCGGAC
GTGCTGTCGTTCATCCAGGGCAAAGTGAACCGCTGA

Upstream 100 bases:

>100_bases
TCCAACGCCACATGCAAGCCAGCCCCGCCCTGTACGCGACCTGCACAGCGCCGTACAATGCCAGGTCATTTCCTGTCTCA
GCAACCGACAACGGCCAACC

Downstream 100 bases:

>100_bases
GTCAACGTGCGCACCTGCTCGCAGGTGCGCCGCTCTTCACTCGCCCAATCCGCATCAGAGATCCCATGCTCAAACGAAGC
ACCCTAAGCCTCGGCAGCGC

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 571; Mature: 571

Protein sequence:

>571_residues
MRTSQFLLATQKETPSDAVVVSHQLMLRAGMIRKLASGLYTWLPMGLRVLRKVEAIVREEMDAAGALEILMPGIQPAELW
QESGRWEQYGPELMRLVDRHNREFCLGPTHEEVITDLARNELNSYKQLPINMYQIQTKFRDEIRPRFGLMRGREFVMKDA
YSFHADHESLQVTYDRMHLAYSNIFTRLGLKFRPVEADNGSIGGAGSHEFHVLAESGEDDIVFSDGSDYAANIEKAEAIP
REKTRPAATEELRLIDTPNTKTIAQLVEGFGLPIEKTVKTLVVHAAEEGKLIALIIRGDHELNEIKASQLEQVANPLVMA
SEAELRDAIGAGAGSLGPLNLPLPCIIDRSVELMSDFSVGANIDDKHYFGVNWERDLPVPTVADLRNVVAGDPSPDGKGT
LEIKRGIEVGHIFQLGTKYSEAMKCQVLGENGKPVNLAMGCYGIGVSRVVAAAIEQNSDENGIIWNDTLAPFQIALVPLR
YETDAVREATDKLYADLTAAGFEVLLDDRDKKTSPGIKFADMELIGIPHRIVVSDRGLAEGNLEYKSRTESQPQAIAVAD
VLSFIQGKVNR

Sequences:

>Translated_571_residues
MRTSQFLLATQKETPSDAVVVSHQLMLRAGMIRKLASGLYTWLPMGLRVLRKVEAIVREEMDAAGALEILMPGIQPAELW
QESGRWEQYGPELMRLVDRHNREFCLGPTHEEVITDLARNELNSYKQLPINMYQIQTKFRDEIRPRFGLMRGREFVMKDA
YSFHADHESLQVTYDRMHLAYSNIFTRLGLKFRPVEADNGSIGGAGSHEFHVLAESGEDDIVFSDGSDYAANIEKAEAIP
REKTRPAATEELRLIDTPNTKTIAQLVEGFGLPIEKTVKTLVVHAAEEGKLIALIIRGDHELNEIKASQLEQVANPLVMA
SEAELRDAIGAGAGSLGPLNLPLPCIIDRSVELMSDFSVGANIDDKHYFGVNWERDLPVPTVADLRNVVAGDPSPDGKGT
LEIKRGIEVGHIFQLGTKYSEAMKCQVLGENGKPVNLAMGCYGIGVSRVVAAAIEQNSDENGIIWNDTLAPFQIALVPLR
YETDAVREATDKLYADLTAAGFEVLLDDRDKKTSPGIKFADMELIGIPHRIVVSDRGLAEGNLEYKSRTESQPQAIAVAD
VLSFIQGKVNR
>Mature_571_residues
MRTSQFLLATQKETPSDAVVVSHQLMLRAGMIRKLASGLYTWLPMGLRVLRKVEAIVREEMDAAGALEILMPGIQPAELW
QESGRWEQYGPELMRLVDRHNREFCLGPTHEEVITDLARNELNSYKQLPINMYQIQTKFRDEIRPRFGLMRGREFVMKDA
YSFHADHESLQVTYDRMHLAYSNIFTRLGLKFRPVEADNGSIGGAGSHEFHVLAESGEDDIVFSDGSDYAANIEKAEAIP
REKTRPAATEELRLIDTPNTKTIAQLVEGFGLPIEKTVKTLVVHAAEEGKLIALIIRGDHELNEIKASQLEQVANPLVMA
SEAELRDAIGAGAGSLGPLNLPLPCIIDRSVELMSDFSVGANIDDKHYFGVNWERDLPVPTVADLRNVVAGDPSPDGKGT
LEIKRGIEVGHIFQLGTKYSEAMKCQVLGENGKPVNLAMGCYGIGVSRVVAAAIEQNSDENGIIWNDTLAPFQIALVPLR
YETDAVREATDKLYADLTAAGFEVLLDDRDKKTSPGIKFADMELIGIPHRIVVSDRGLAEGNLEYKSRTESQPQAIAVAD
VLSFIQGKVNR

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=219, Percent_Identity=47.945205479452, Blast_Score=221, Evalue=2e-57,
Organism=Escherichia coli, GI1786392, Length=573, Percent_Identity=67.3647469458988, Blast_Score=811, Evalue=0.0,
Organism=Escherichia coli, GI1788013, Length=129, Percent_Identity=29.4573643410853, Blast_Score=66, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI115532348, Length=210, Percent_Identity=43.3333333333333, Blast_Score=174, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI193203271, Length=86, Percent_Identity=39.5348837209302, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320931, Length=536, Percent_Identity=31.5298507462687, Blast_Score=248, Evalue=3e-66,
Organism=Drosophila melanogaster, GI24656200, Length=233, Percent_Identity=37.3390557939914, Blast_Score=170, Evalue=2e-42,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 63172; Mature: 63172

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTSQFLLATQKETPSDAVVVSHQLMLRAGMIRKLASGLYTWLPMGLRVLRKVEAIVREE
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MDAAGALEILMPGIQPAELWQESGRWEQYGPELMRLVDRHNREFCLGPTHEEVITDLARN
HCCCCCHHHHCCCCCHHHHHHHCCCHHHCCHHHHHHHHHCCCCEECCCCHHHHHHHHHHH
ELNSYKQLPINMYQIQTKFRDEIRPRFGLMRGREFVMKDAYSFHADHESLQVTYDRMHLA
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCEEEEHHHHHHH
YSNIFTRLGLKFRPVEADNGSIGGAGSHEFHVLAESGEDDIVFSDGSDYAANIEKAEAIP
HHHHHHHCCCEEEEEECCCCCCCCCCCCEEEEEEECCCCCEEEECCCCHHHCCHHHHCCC
REKTRPAATEELRLIDTPNTKTIAQLVEGFGLPIEKTVKTLVVHAAEEGKLIALIIRGDH
CCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHEECCCCCEEEEEEECCC
ELNEIKASQLEQVANPLVMASEAELRDAIGAGAGSLGPLNLPLPCIIDRSVELMSDFSVG
CHHHHHHHHHHHHHCCEEEECHHHHHHHHCCCCCCCCCCCCCCCCEECCCHHHHHCCCCC
ANIDDKHYFGVNWERDLPVPTVADLRNVVAGDPSPDGKGTLEIKRGIEVGHIFQLGTKYS
CCCCCCEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCEEHHHHHCCHHH
EAMKCQVLGENGKPVNLAMGCYGIGVSRVVAAAIEQNSDENGIIWNDTLAPFQIALVPLR
HCEEEEEECCCCCEEEEEEEEHHCCHHHHHHHHHHCCCCCCCEEECCCCCCEEEEEEEEE
YETDAVREATDKLYADLTAAGFEVLLDDRDKKTSPGIKFADMELIGIPHRIVVSDRGLAE
CCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCEEECEEEECCCEEEEEECCCCCC
GNLEYKSRTESQPQAIAVADVLSFIQGKVNR
CCCCHHHCCCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRTSQFLLATQKETPSDAVVVSHQLMLRAGMIRKLASGLYTWLPMGLRVLRKVEAIVREE
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MDAAGALEILMPGIQPAELWQESGRWEQYGPELMRLVDRHNREFCLGPTHEEVITDLARN
HCCCCCHHHHCCCCCHHHHHHHCCCHHHCCHHHHHHHHHCCCCEECCCCHHHHHHHHHHH
ELNSYKQLPINMYQIQTKFRDEIRPRFGLMRGREFVMKDAYSFHADHESLQVTYDRMHLA
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCEEEEHHHHHHH
YSNIFTRLGLKFRPVEADNGSIGGAGSHEFHVLAESGEDDIVFSDGSDYAANIEKAEAIP
HHHHHHHCCCEEEEEECCCCCCCCCCCCEEEEEEECCCCCEEEECCCCHHHCCHHHHCCC
REKTRPAATEELRLIDTPNTKTIAQLVEGFGLPIEKTVKTLVVHAAEEGKLIALIIRGDH
CCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHEECCCCCEEEEEEECCC
ELNEIKASQLEQVANPLVMASEAELRDAIGAGAGSLGPLNLPLPCIIDRSVELMSDFSVG
CHHHHHHHHHHHHHCCEEEECHHHHHHHHCCCCCCCCCCCCCCCCEECCCHHHHHCCCCC
ANIDDKHYFGVNWERDLPVPTVADLRNVVAGDPSPDGKGTLEIKRGIEVGHIFQLGTKYS
CCCCCCEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCEEHHHHHCCHHH
EAMKCQVLGENGKPVNLAMGCYGIGVSRVVAAAIEQNSDENGIIWNDTLAPFQIALVPLR
HCEEEEEECCCCCEEEEEEEEHHCCHHHHHHHHHHCCCCCCCEEECCCCCCEEEEEEEEE
YETDAVREATDKLYADLTAAGFEVLLDDRDKKTSPGIKFADMELIGIPHRIVVSDRGLAE
CCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCEEECEEEECCCEEEEEECCCCCC
GNLEYKSRTESQPQAIAVADVLSFIQGKVNR
CCCCHHHCCCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA