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The map label for this gene is rimO [H]

Identifier: 66044640

GI number: 66044640

Start: 1572595

End: 1573938

Strand: Reverse

Name: rimO [H]

Synonym: Psyr_1392

Alternate gene names: 66044640

Gene position: 1573938-1572595 (Counterclockwise)

Preceding gene: 66044641

Following gene: 66044639

Centisome position: 25.83

GC content: 61.16

Gene sequence:

>1344_bases
ATGTCCACCGTCACCACGCCGTCCGCCCCCAAGGTTGGATTCGTTTCACTGGGTTGCCCAAAGGCACTCGTTGACTCTGA
ACGCATCCTGACCCAGCTGCGTATGGAAGGTTACGAAGTCGTTGCGACCTACGAGGACGCCGACGTGGTCGTGGTCAACA
CCTGTGGCTTCATCGATACAGCCAAGGCCGAGTCGCTGGAAGTGATCGGCGAAGCCATCAAGGAAAACGGCAAGGTCATC
GTGACCGGCTGCATGGGCGTCGATGCGAGTGTCATTCGCAATGTGCACCCGAGCGTGCTGTCGGTGACCGGCCCGCAGCA
GTACGAGCAGGTGGTCAACGCAGTGCACGATGTGGTGCCGCCGCGCAAGGATCACAACCCGTTGATCGACCTGGTGCCGC
CACAAGGCGTCAAGCTGACCCCGCGTCACTACGCGTATCTGAAAATTTCCGAAGGCTGCAACCACAGTTGCAGCTTCTGC
ATCATCCCGTCGATGCGCGGCAAGCTGGTCAGCCGCCCGGTGGGTGATGTACTGGACGAGGCCAAGCGCCTGGTCAAATC
CGGGGTGAAGGAACTGCTGGTCATTTCCCAGGACACCAGCGCCTATGGCGTTGACGTCAAATACCGCACCGGTTTCTGGG
ACGGTCAGCCGGTCAAAACCCGCATGACCGAGCTGTGCCAGGCGCTGGGCTCGATGGGTGTCTGGGTGCGCCTGCACTAT
GTCTACCCGTACCCGCACGTCGACGAACTGATCCCGTTGATGGCCGCGGGCAAGATCCTGCCGTACCTGGACATCCCGTT
CCAGCACGCCAGCCCGAAAATCCTCAAGCTGATGAAGCGTCCGGCCTTTGAAGACAAGACGCTGGCGCGCATCAAGAACT
GGCGCGAGCAGTGCCCGGACCTGATCATCCGCTCGACCTTCATCGTCGGTTTTCCCGGTGAAACCGAAGAAGACTTCCAG
TACCTGCTGGACTGGCTGACCGAAGCGCAGCTGGACCGGGTTGGCTGCTTCCAGTACTCGCCGGTTGAAGGCGCGCCCGC
CAATCTGCTGGACGCGGCCATCGTGCCGGACGACGTCAAGCAGGATCGCTGGGATCGCTTCATGGCTCATCAGCAGGCAA
TCAGCGCTGCGCGCCTGCAGATGAAGATCGGCAAGGAAATCGAAGTGCTGATCGACGAAGTCGACGATCGTGGTGCCGTG
GGCCGCTGCTTCTTCGACGCCCCGGAAATCGACGGCAACGTGTTCATCGGCCTGGACGACGGCAGCACCGTTCAGCCCGG
GGACAAGATCATGTGCCGGGTGACCGATGCCGACGAATACGACCTGTGGGCCGAGATGCTCTAA

Upstream 100 bases:

>100_bases
ACTCGCCGCTAGAATTGCGCACTTTTTGATCAGAGGCGCAGCAAGCGCCCAGTCAGCGCCTGCGCTTTGCGTCCGGGCGC
CCTTCACGCCGAGGTTTGCC

Downstream 100 bases:

>100_bases
GCGCTCTCCCTGGTCGCAAGCGAAAGCCCTGCCCTTTAACCGGCAGGGCTTTTTCGTCTCGGGCTGTTGTCTATCCAGGA
CCGGCTTAAAGGAGGACGCG

Product: ribosomal protein S12 methylthiotransferase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO [H]

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MSTVTTPSAPKVGFVSLGCPKALVDSERILTQLRMEGYEVVATYEDADVVVVNTCGFIDTAKAESLEVIGEAIKENGKVI
VTGCMGVDASVIRNVHPSVLSVTGPQQYEQVVNAVHDVVPPRKDHNPLIDLVPPQGVKLTPRHYAYLKISEGCNHSCSFC
IIPSMRGKLVSRPVGDVLDEAKRLVKSGVKELLVISQDTSAYGVDVKYRTGFWDGQPVKTRMTELCQALGSMGVWVRLHY
VYPYPHVDELIPLMAAGKILPYLDIPFQHASPKILKLMKRPAFEDKTLARIKNWREQCPDLIIRSTFIVGFPGETEEDFQ
YLLDWLTEAQLDRVGCFQYSPVEGAPANLLDAAIVPDDVKQDRWDRFMAHQQAISAARLQMKIGKEIEVLIDEVDDRGAV
GRCFFDAPEIDGNVFIGLDDGSTVQPGDKIMCRVTDADEYDLWAEML

Sequences:

>Translated_447_residues
MSTVTTPSAPKVGFVSLGCPKALVDSERILTQLRMEGYEVVATYEDADVVVVNTCGFIDTAKAESLEVIGEAIKENGKVI
VTGCMGVDASVIRNVHPSVLSVTGPQQYEQVVNAVHDVVPPRKDHNPLIDLVPPQGVKLTPRHYAYLKISEGCNHSCSFC
IIPSMRGKLVSRPVGDVLDEAKRLVKSGVKELLVISQDTSAYGVDVKYRTGFWDGQPVKTRMTELCQALGSMGVWVRLHY
VYPYPHVDELIPLMAAGKILPYLDIPFQHASPKILKLMKRPAFEDKTLARIKNWREQCPDLIIRSTFIVGFPGETEEDFQ
YLLDWLTEAQLDRVGCFQYSPVEGAPANLLDAAIVPDDVKQDRWDRFMAHQQAISAARLQMKIGKEIEVLIDEVDDRGAV
GRCFFDAPEIDGNVFIGLDDGSTVQPGDKIMCRVTDADEYDLWAEML
>Mature_446_residues
STVTTPSAPKVGFVSLGCPKALVDSERILTQLRMEGYEVVATYEDADVVVVNTCGFIDTAKAESLEVIGEAIKENGKVIV
TGCMGVDASVIRNVHPSVLSVTGPQQYEQVVNAVHDVVPPRKDHNPLIDLVPPQGVKLTPRHYAYLKISEGCNHSCSFCI
IPSMRGKLVSRPVGDVLDEAKRLVKSGVKELLVISQDTSAYGVDVKYRTGFWDGQPVKTRMTELCQALGSMGVWVRLHYV
YPYPHVDELIPLMAAGKILPYLDIPFQHASPKILKLMKRPAFEDKTLARIKNWREQCPDLIIRSTFIVGFPGETEEDFQY
LLDWLTEAQLDRVGCFQYSPVEGAPANLLDAAIVPDDVKQDRWDRFMAHQQAISAARLQMKIGKEIEVLIDEVDDRGAVG
RCFFDAPEIDGNVFIGLDDGSTVQPGDKIMCRVTDADEYDLWAEML

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12 [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI93277076, Length=420, Percent_Identity=27.1428571428571, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI28872782, Length=481, Percent_Identity=24.3243243243243, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI28872784, Length=481, Percent_Identity=24.3243243243243, Blast_Score=133, Evalue=3e-31,
Organism=Escherichia coli, GI1787057, Length=444, Percent_Identity=70.2702702702703, Blast_Score=644, Evalue=0.0,
Organism=Escherichia coli, GI1786882, Length=399, Percent_Identity=28.8220551378446, Blast_Score=147, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17553146, Length=470, Percent_Identity=25.9574468085106, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI71996771, Length=260, Percent_Identity=28.0769230769231, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21356207, Length=480, Percent_Identity=24.7916666666667, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI19922432, Length=471, Percent_Identity=23.5668789808917, Blast_Score=114, Evalue=9e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 49628; Mature: 49496

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS50926 TRAM ; PS00430 TONB_DEPENDENT_REC_1 ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTVTTPSAPKVGFVSLGCPKALVDSERILTQLRMEGYEVVATYEDADVVVVNTCGFIDT
CCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCEEEEECCCCCCC
AKAESLEVIGEAIKENGKVIVTGCMGVDASVIRNVHPSVLSVTGPQQYEQVVNAVHDVVP
CCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHCCCCCEEEECCHHHHHHHHHHHHHHCC
PRKDHNPLIDLVPPQGVKLTPRHYAYLKISEGCNHSCSFCIIPSMRGKLVSRPVGDVLDE
CCCCCCCEEEECCCCCCEECCCEEEEEEECCCCCCCCCEEEECCCCCHHHCCCHHHHHHH
AKRLVKSGVKELLVISQDTSAYGVDVKYRTGFWDGQPVKTRMTELCQALGSMGVWVRLHY
HHHHHHHHHHHEEEEECCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEEE
VYPYPHVDELIPLMAAGKILPYLDIPFQHASPKILKLMKRPAFEDKTLARIKNWREQCPD
EECCCCHHHHHHHHHCCCCCCEECCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LIIRSTFIVGFPGETEEDFQYLLDWLTEAQLDRVGCFQYSPVEGAPANLLDAAIVPDDVK
HEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCHHHHHHHCCCCHHH
QDRWDRFMAHQQAISAARLQMKIGKEIEVLIDEVDDRGAVGRCFFDAPEIDGNVFIGLDD
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCEEEEECCCCCCCEEEEEECC
GSTVQPGDKIMCRVTDADEYDLWAEML
CCCCCCCCCEEEEEECCCCHHHHHHCC
>Mature Secondary Structure 
STVTTPSAPKVGFVSLGCPKALVDSERILTQLRMEGYEVVATYEDADVVVVNTCGFIDT
CCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCEEEEECCCCCCC
AKAESLEVIGEAIKENGKVIVTGCMGVDASVIRNVHPSVLSVTGPQQYEQVVNAVHDVVP
CCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHCCCCCEEEECCHHHHHHHHHHHHHHCC
PRKDHNPLIDLVPPQGVKLTPRHYAYLKISEGCNHSCSFCIIPSMRGKLVSRPVGDVLDE
CCCCCCCEEEECCCCCCEECCCEEEEEEECCCCCCCCCEEEECCCCCHHHCCCHHHHHHH
AKRLVKSGVKELLVISQDTSAYGVDVKYRTGFWDGQPVKTRMTELCQALGSMGVWVRLHY
HHHHHHHHHHHEEEEECCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEEE
VYPYPHVDELIPLMAAGKILPYLDIPFQHASPKILKLMKRPAFEDKTLARIKNWREQCPD
EECCCCHHHHHHHHHCCCCCCEECCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LIIRSTFIVGFPGETEEDFQYLLDWLTEAQLDRVGCFQYSPVEGAPANLLDAAIVPDDVK
HEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCHHHHHHHCCCCHHH
QDRWDRFMAHQQAISAARLQMKIGKEIEVLIDEVDDRGAVGRCFFDAPEIDGNVFIGLDD
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCEEEEECCCCCCCEEEEEECC
GSTVQPGDKIMCRVTDADEYDLWAEML
CCCCCCCCCEEEEEECCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA