Click here to switch to the map view.

The map label for this gene is rsuA [C]

Identifier: 66044638

GI number: 66044638

Start: 1571249

End: 1571959

Strand: Reverse

Name: rsuA [C]

Synonym: Psyr_1390

Alternate gene names: 66044638

Gene position: 1571959-1571249 (Counterclockwise)

Preceding gene: 66044639

Following gene: 66044637

Centisome position: 25.8

GC content: 60.76

Gene sequence:

>711_bases
ATGACAGACCCCATTCGCCTTTCCAAACGCCTTATCGAGCTGGTCGGTTGCTCCCGCCGGGAGGCCGAACTGTTTATCGA
GGGTGGCTGGGTCACTGTGGACGGCGAGGTCATCGAAGAACCGCACTTCAAGGTCAGCACCCAGAAAATCGAGCTCAGCC
CGGACGCCAAGGCCGACTCTCCGGAGCCTGTGACGATCATCCTGCACAAACCCGCCTCGGCAAGCGCCGAAGAAGCGTTG
CAGATGATCACGCCGGGTACGCTCTCCGTAGAGCACAGCTACAGCAAGCGTCCGCTCAAGGGCCATTTCATGCGCCTTGA
GGCGATTTCAAGCCTGCAGGCCAACGCCAGCGGCTTGATGGTGTTCAGCCAGGACTGGAAAATCCTGCGCAAGCTCACCG
ATGACCGCAGCAAGATCGAGCAGGAATACGTGGTGGAAATCTCGGGCGAGATGGCTGCTCATGGCCTCAACCGGCTGAAC
CACGGCCTGACCTACAAAGGCAAGGAGCTGCCAGCCGTGAAAGCCAGCTGGCAGAACGAGAGCCGCCTGCGCTTTGCAAT
GAAAAACCCGCAGCCCGGCATCATCGCCCTGCTGTGCGAGGCCGTCGGCCTGAAGATCGTCGCTATCCGCCGCATCCGCA
TCGGCGGCGTGTCCATGGGCAAACTGCCCGAGGGCCAGTGGCGCTACATGACGGCCAAGGAAAAATTCTGA

Upstream 100 bases:

>100_bases
ATCACTGACACCGATTGCAGCGCAAATGCAATGTTGCCGCGATTAACCGTCGCCGGGCGGTTACAATGGCGACTTTATTT
TAGCAAACGGCTTTTTTGTC

Downstream 100 bases:

>100_bases
CCCTCGCGCCACCCACCTTATTACAGCGCGCATGACCACCGTCATGCGCTGCCCTACGACTTATCAGGAAACACGCCTCA
TGATGAACAACGATGTACTG

Product: RNA-binding S4

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 236; Mature: 235

Protein sequence:

>236_residues
MTDPIRLSKRLIELVGCSRREAELFIEGGWVTVDGEVIEEPHFKVSTQKIELSPDAKADSPEPVTIILHKPASASAEEAL
QMITPGTLSVEHSYSKRPLKGHFMRLEAISSLQANASGLMVFSQDWKILRKLTDDRSKIEQEYVVEISGEMAAHGLNRLN
HGLTYKGKELPAVKASWQNESRLRFAMKNPQPGIIALLCEAVGLKIVAIRRIRIGGVSMGKLPEGQWRYMTAKEKF

Sequences:

>Translated_236_residues
MTDPIRLSKRLIELVGCSRREAELFIEGGWVTVDGEVIEEPHFKVSTQKIELSPDAKADSPEPVTIILHKPASASAEEAL
QMITPGTLSVEHSYSKRPLKGHFMRLEAISSLQANASGLMVFSQDWKILRKLTDDRSKIEQEYVVEISGEMAAHGLNRLN
HGLTYKGKELPAVKASWQNESRLRFAMKNPQPGIIALLCEAVGLKIVAIRRIRIGGVSMGKLPEGQWRYMTAKEKF
>Mature_235_residues
TDPIRLSKRLIELVGCSRREAELFIEGGWVTVDGEVIEEPHFKVSTQKIELSPDAKADSPEPVTIILHKPASASAEEALQ
MITPGTLSVEHSYSKRPLKGHFMRLEAISSLQANASGLMVFSQDWKILRKLTDDRSKIEQEYVVEISGEMAAHGLNRLNH
GLTYKGKELPAVKASWQNESRLRFAMKNPQPGIIALLCEAVGLKIVAIRRIRIGGVSMGKLPEGQWRYMTAKEKF

Specific function: Responsible For Synthesis Of Pseudouridine From Uracil-516 In 16s Ribosomal RNA. [C]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 26347; Mature: 26216

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: PS50889 S4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDPIRLSKRLIELVGCSRREAELFIEGGWVTVDGEVIEEPHFKVSTQKIELSPDAKADS
CCCHHHHHHHHHHHHCCCCCCCEEEEECCEEEECCCEECCCCEEEEEEEEEECCCCCCCC
PEPVTIILHKPASASAEEALQMITPGTLSVEHSYSKRPLKGHFMRLEAISSLQANASGLM
CCCEEEEEECCCCCCHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEE
VFSQDWKILRKLTDDRSKIEQEYVVEISGEMAAHGLNRLNHGLTYKGKELPAVKASWQNE
EECCCHHHHHHHHCCHHHHCCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCEEECCCCCC
SRLRFAMKNPQPGIIALLCEAVGLKIVAIRRIRIGGVSMGKLPEGQWRYMTAKEKF
CEEEEEECCCCCCHHHHHHHHHCEEEEEEEEEEECCCCCCCCCCCCEEEEECCCCC
>Mature Secondary Structure 
TDPIRLSKRLIELVGCSRREAELFIEGGWVTVDGEVIEEPHFKVSTQKIELSPDAKADS
CCHHHHHHHHHHHHCCCCCCCEEEEECCEEEECCCEECCCCEEEEEEEEEECCCCCCCC
PEPVTIILHKPASASAEEALQMITPGTLSVEHSYSKRPLKGHFMRLEAISSLQANASGLM
CCCEEEEEECCCCCCHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEE
VFSQDWKILRKLTDDRSKIEQEYVVEISGEMAAHGLNRLNHGLTYKGKELPAVKASWQNE
EECCCHHHHHHHHCCHHHHCCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCEEECCCCCC
SRLRFAMKNPQPGIIALLCEAVGLKIVAIRRIRIGGVSMGKLPEGQWRYMTAKEKF
CEEEEEECCCCCCHHHHHHHHHCEEEEEEEEEEECCCCCCCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]