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The map label for this gene is HrcN [H]

Identifier: 66044464

GI number: 66044464

Start: 1369057

End: 1370406

Strand: Reverse

Name: HrcN [H]

Synonym: Psyr_1213

Alternate gene names: 66044464

Gene position: 1370406-1369057 (Counterclockwise)

Preceding gene: 66044465

Following gene: 66044463

Centisome position: 22.49

GC content: 65.7

Gene sequence:

>1350_bases
GTGAATGCAGCACTGAACCTGTGGAAAGACGCCCACGCCAGGCGTTTGAGCGAGTACTGCGCGGTGCGCGTCATTGGCCG
GGTCAGCGCCGTGCGCGGGATTCTGCTGGAGTGCAAGATTCCGTCGGCCAAGGTCGGCGATCTCTGTGAAGTGAGCAAAG
CCGACGGCTCGTTGCTGCTGGCCGAAATCGTCGGCTTCACCCAGGAATGCACGCTGCTCAGTGCCTTGGGCCCACCCGAC
GGTATTCAGGTCGGCGCGCCGATCCGCCCGCTGGGCGTGGCACACCGGATCGGCGTCGATGACAGCCTGCTCGGCTGTGT
ACTGGACGGTTTCGGGCGCCCGCTGATGGGCGATTGCCTCGGTGCTTTCGCCGGCCCCGAGGACCGCCGCACGACGCTGC
CGGTGATCGCCGACGCCCTGCCGCCGACCCAGCGACCGCGTATCACCCGGGCGTTACCCACCGGGATACGCGCCATCGAC
AGTGCGATTCTGCTCGGCGAGGGCCAGCGTGTCGGGCTGTTCGCCGGTGCGGGCTGCGGCAAGACCACGCTGATGGCCGA
ACTGGCGCGCAACATGGATTGTGACGTCATCGTTTTCGGCCTGATCGGCGAACGGGGCCGCGAGCTGCGCGAGTTTCTCG
ACCATGAACTGGATGAAACCCTGCGCCGCCGCTCGGTGCTGGTCTGTGCGACATCCGATCGTTCCAGCATGGAGCGCGCC
CGCGCTGCGTTCACCGCGACCGCCATTGCCGAAGCCTTTCGCGCCCGCGGCCAGAAAGTGTTGTTGCTGCTCGACTCGCT
GACCCGCTTCGCCCGCGCGCAACGGGAAATCGGCATTGCCTCGGGCGAGCCGCTGGGGCGCGGCGGTCTGCCGCCCTCGG
TGTACACCCTGCTGCCACGCCTGGTGGAACGCGCCGGGATGAGCGAAAACGGTTCGATCACCGCGCTTTACACGGTACTG
ATCGAACAGGACTCGATGAACGATCCGGTCGCCGACGAAGTGCGCTCGTTGCTCGACGGGCACATCGTACTGTCGCGCAA
ACTGGCTGAACGCGGGCACTACCCGGCGATCGATGTGTCGGCCAGCATCAGCCGGATCCTGAGCAACGTCACCGGTCGCG
AACATCAACGGGCGAACAATCGCCTGCGTCAGTTGCTGGCCGCCTATAAACAAGTGGAAATGCTCCTGCGCCTGGGCGAA
TACCAGGCCGGGGCCGACCCGGTCACCGACTGTGCCGTGCAACTGAACGAGGCTATAAACGCGTTCCTGCGCCAGGACCT
GCGTGAGCCCGTGCCGTTGCAGGAAACCCTCGACAGGCTGCTGCAACTCACCTCTCAACTGCCGGAGTAG

Upstream 100 bases:

>100_bases
GACGTGTGTACGTAGGCGATGAAGTGGATGGCCTGCGCCTCACCCGTATCGACAATCAGCGTCTGCAATTCGACGGTAAT
CGCCATATCGAGGTGAACTG

Downstream 100 bases:

>100_bases
CCATGGACGAACCACTGGAAGACGACCCGCAACAGGTCGCTCTGCAGCAGGTGATCGGGCTGCTCACGCCACTGCGCCAA
CATCGTCAGGCCAGTGCCGA

Product: type III secretion cytoplasmic ATPase HrcN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MNAALNLWKDAHARRLSEYCAVRVIGRVSAVRGILLECKIPSAKVGDLCEVSKADGSLLLAEIVGFTQECTLLSALGPPD
GIQVGAPIRPLGVAHRIGVDDSLLGCVLDGFGRPLMGDCLGAFAGPEDRRTTLPVIADALPPTQRPRITRALPTGIRAID
SAILLGEGQRVGLFAGAGCGKTTLMAELARNMDCDVIVFGLIGERGRELREFLDHELDETLRRRSVLVCATSDRSSMERA
RAAFTATAIAEAFRARGQKVLLLLDSLTRFARAQREIGIASGEPLGRGGLPPSVYTLLPRLVERAGMSENGSITALYTVL
IEQDSMNDPVADEVRSLLDGHIVLSRKLAERGHYPAIDVSASISRILSNVTGREHQRANNRLRQLLAAYKQVEMLLRLGE
YQAGADPVTDCAVQLNEAINAFLRQDLREPVPLQETLDRLLQLTSQLPE

Sequences:

>Translated_449_residues
MNAALNLWKDAHARRLSEYCAVRVIGRVSAVRGILLECKIPSAKVGDLCEVSKADGSLLLAEIVGFTQECTLLSALGPPD
GIQVGAPIRPLGVAHRIGVDDSLLGCVLDGFGRPLMGDCLGAFAGPEDRRTTLPVIADALPPTQRPRITRALPTGIRAID
SAILLGEGQRVGLFAGAGCGKTTLMAELARNMDCDVIVFGLIGERGRELREFLDHELDETLRRRSVLVCATSDRSSMERA
RAAFTATAIAEAFRARGQKVLLLLDSLTRFARAQREIGIASGEPLGRGGLPPSVYTLLPRLVERAGMSENGSITALYTVL
IEQDSMNDPVADEVRSLLDGHIVLSRKLAERGHYPAIDVSASISRILSNVTGREHQRANNRLRQLLAAYKQVEMLLRLGE
YQAGADPVTDCAVQLNEAINAFLRQDLREPVPLQETLDRLLQLTSQLPE
>Mature_449_residues
MNAALNLWKDAHARRLSEYCAVRVIGRVSAVRGILLECKIPSAKVGDLCEVSKADGSLLLAEIVGFTQECTLLSALGPPD
GIQVGAPIRPLGVAHRIGVDDSLLGCVLDGFGRPLMGDCLGAFAGPEDRRTTLPVIADALPPTQRPRITRALPTGIRAID
SAILLGEGQRVGLFAGAGCGKTTLMAELARNMDCDVIVFGLIGERGRELREFLDHELDETLRRRSVLVCATSDRSSMERA
RAAFTATAIAEAFRARGQKVLLLLDSLTRFARAQREIGIASGEPLGRGGLPPSVYTLLPRLVERAGMSENGSITALYTVL
IEQDSMNDPVADEVRSLLDGHIVLSRKLAERGHYPAIDVSASISRILSNVTGREHQRANNRLRQLLAAYKQVEMLLRLGE
YQAGADPVTDCAVQLNEAINAFLRQDLREPVPLQETLDRLLQLTSQLPE

Specific function: ATPase which is probably required to catalyze the translocation of virulent proteins into the host cell during infection [H]

COG id: COG1157

COG function: function code NU; Flagellar biosynthesis/type III secretory pathway ATPase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATPase alpha/beta chains family [H]

Homologues:

Organism=Homo sapiens, GI32189394, Length=478, Percent_Identity=28.2426778242678, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI50345984, Length=373, Percent_Identity=26.5415549597855, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI4757810, Length=373, Percent_Identity=26.5415549597855, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI19913428, Length=378, Percent_Identity=28.3068783068783, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI19913426, Length=323, Percent_Identity=27.8637770897833, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI19913424, Length=263, Percent_Identity=28.8973384030418, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI1788251, Length=443, Percent_Identity=41.7607223476298, Blast_Score=300, Evalue=2e-82,
Organism=Escherichia coli, GI1790170, Length=381, Percent_Identity=29.9212598425197, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI1790172, Length=380, Percent_Identity=25.5263157894737, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1790217, Length=248, Percent_Identity=29.0322580645161, Blast_Score=75, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI25144756, Length=336, Percent_Identity=30.0595238095238, Blast_Score=127, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI71988080, Length=371, Percent_Identity=26.6846361185984, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71988063, Length=371, Percent_Identity=26.6846361185984, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17570191, Length=442, Percent_Identity=25.5656108597285, Blast_Score=105, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI71988074, Length=361, Percent_Identity=26.3157894736842, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17510931, Length=325, Percent_Identity=28, Blast_Score=102, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17565854, Length=263, Percent_Identity=28.8973384030418, Blast_Score=96, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6322581, Length=408, Percent_Identity=29.9019607843137, Blast_Score=139, Evalue=9e-34,
Organism=Saccharomyces cerevisiae, GI6319370, Length=372, Percent_Identity=27.4193548387097, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6319603, Length=389, Percent_Identity=28.0205655526992, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6320016, Length=277, Percent_Identity=28.158844765343, Blast_Score=84, Evalue=5e-17,
Organism=Drosophila melanogaster, GI28574560, Length=340, Percent_Identity=33.2352941176471, Blast_Score=138, Evalue=7e-33,
Organism=Drosophila melanogaster, GI24638766, Length=388, Percent_Identity=29.1237113402062, Blast_Score=138, Evalue=8e-33,
Organism=Drosophila melanogaster, GI281361666, Length=455, Percent_Identity=26.5934065934066, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI24646341, Length=455, Percent_Identity=26.5934065934066, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI17136796, Length=455, Percent_Identity=26.5934065934066, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI24658560, Length=376, Percent_Identity=26.5957446808511, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24583988, Length=268, Percent_Identity=30.5970149253731, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24583986, Length=268, Percent_Identity=30.5970149253731, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24583984, Length=268, Percent_Identity=30.5970149253731, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI20129479, Length=255, Percent_Identity=28.6274509803922, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24583992, Length=268, Percent_Identity=30.5970149253731, Blast_Score=102, Evalue=4e-22,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020003
- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR004100
- InterPro:   IPR005714 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF02874 ATP-synt_ab_N [H]

EC number: =3.6.3.14 [H]

Molecular weight: Translated: 48618; Mature: 48618

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: PS00152 ATPASE_ALPHA_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAALNLWKDAHARRLSEYCAVRVIGRVSAVRGILLECKIPSAKVGDLCEVSKADGSLLL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHCCCCCCCHH
AEIVGFTQECTLLSALGPPDGIQVGAPIRPLGVAHRIGVDDSLLGCVLDGFGRPLMGDCL
HHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHCCCCHHHHHH
GAFAGPEDRRTTLPVIADALPPTQRPRITRALPTGIRAIDSAILLGEGQRVGLFAGAGCG
HHHCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHEECCCCEEEEEECCCCC
KTTLMAELARNMDCDVIVFGLIGERGRELREFLDHELDETLRRRSVLVCATSDRSSMERA
HHHHHHHHHHCCCCCEEEEEHHCCCHHHHHHHHHHHHHHHHHHCEEEEEECCCHHHHHHH
RAAFTATAIAEAFRARGQKVLLLLDSLTRFARAQREIGIASGEPLGRGGLPPSVYTLLPR
HHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHH
LVERAGMSENGSITALYTVLIEQDSMNDPVADEVRSLLDGHIVLSRKLAERGHYPAIDVS
HHHHCCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCEEEHH
ASISRILSNVTGREHQRANNRLRQLLAAYKQVEMLLRLGEYQAGADPVTDCAVQLNEAIN
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
AFLRQDLREPVPLQETLDRLLQLTSQLPE
HHHHHHHCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNAALNLWKDAHARRLSEYCAVRVIGRVSAVRGILLECKIPSAKVGDLCEVSKADGSLLL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHCCCCCCCHH
AEIVGFTQECTLLSALGPPDGIQVGAPIRPLGVAHRIGVDDSLLGCVLDGFGRPLMGDCL
HHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHCCCCHHHHHH
GAFAGPEDRRTTLPVIADALPPTQRPRITRALPTGIRAIDSAILLGEGQRVGLFAGAGCG
HHHCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHEECCCCEEEEEECCCCC
KTTLMAELARNMDCDVIVFGLIGERGRELREFLDHELDETLRRRSVLVCATSDRSSMERA
HHHHHHHHHHCCCCCEEEEEHHCCCHHHHHHHHHHHHHHHHHHCEEEEEECCCHHHHHHH
RAAFTATAIAEAFRARGQKVLLLLDSLTRFARAQREIGIASGEPLGRGGLPPSVYTLLPR
HHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHH
LVERAGMSENGSITALYTVLIEQDSMNDPVADEVRSLLDGHIVLSRKLAERGHYPAIDVS
HHHHCCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCEEEHH
ASISRILSNVTGREHQRANNRLRQLLAAYKQVEMLLRLGEYQAGADPVTDCAVQLNEAIN
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
AFLRQDLREPVPLQETLDRLLQLTSQLPE
HHHHHHHCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12734178 [H]