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The map label for this gene is HrpS [H]

Identifier: 66044442

GI number: 66044442

Start: 1352287

End: 1353195

Strand: Direct

Name: HrpS [H]

Synonym: Psyr_1191

Alternate gene names: 66044442

Gene position: 1352287-1353195 (Clockwise)

Preceding gene: 66044441

Following gene: 66044443

Centisome position: 22.19

GC content: 60.95

Gene sequence:

>909_bases
ATGAATCTCGATGATGAATTTGACGATCACCTGGATGCGGAGCGCGTACCCAATCTGGGGATCGTCGCCGAAAGTATTTC
GCAGCTCGGCATTGATGTCTTGCTGTCGGGCGAGACCGGTACCGGTAAAGACACCATTGCCCGGCGCATTCACAACATGT
CCGGGCGCCAGGGTCGTTTCGTCCCGATGAACTGCGCAGCCATTCCGGAATCGCTCGCCGAGAGCGAGCTGTTCGGAGTG
GTGAGTGGGGCCTATACCGGCGCCGACCGCTCCCGAATGGGCTACATCGAGGCGGCGCAGGGCGGAACCTTGTACCTGGA
CGAGATCGATAGCATGCCGCTCGCCCTCCAGGCCAAGCTGCTGAGGGTGCTTGAAACCCGTGCACTGGAGCGTCTGGGTT
CGACCTCCACGATCAACCTGGATATCTGTGTGATTGCCTCGGCCCAGGCCTGTCTGGACGACGCCGTCGAAGAGGGGAAG
TTTCGGCGCGACCTGTACTTTCGCCTGAACGTGCTGACCCTCAAACTGCCGCCGCTGCGCGATCAGCCCGAGCGTATCCT
GCCGCTGTTCACCCGGTTTGTGGCGGCGTCGGCCAAAGAACTGAGCGTCCCGATTCCCGATGTATGTCCGTTGCTTCAGC
AGGTACTGACGGGGCATCACTGGCCCGGCAATATCCGCGAGCTCAAGGCCGCTGCAAAGCGCCATGTATTGGGCTTTCCT
CTGCTTGGGGCTGACTCGCAGACCGAAGAGCACCTGGCGTGCGGGCTCAAGTTCCAGCTGCGGGCGATCGAAAAGGCCTT
GATTCAGCAAGCGCTCAAGCGCCACCGCAATTGCATCGACGCGGCCAGCCTGGAGCTCGATATCCCACGTCGTACGCTCT
ACCGCCGCATCAAGGAATTGAGCATCTGA

Upstream 100 bases:

>100_bases
CCGCATGAAAGAGCTGGGTGTTGCTTCGCATATCGACCTGGTAGCCGAGTCCTGAATCGCTGCTGCGCTCGCGCAACGCT
GATGCCATTGGAGTCATGAA

Downstream 100 bases:

>100_bases
TTTTTTGCAGAAGTTCTGGAACCGATTCGCGGACGCATGCCACCTAGCTGTACCAAGCAATTACGCTGGTACAGACGAAG
GGGTATGACGTTATGACTAT

Product: type III transcriptional regulator HrpS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MNLDDEFDDHLDAERVPNLGIVAESISQLGIDVLLSGETGTGKDTIARRIHNMSGRQGRFVPMNCAAIPESLAESELFGV
VSGAYTGADRSRMGYIEAAQGGTLYLDEIDSMPLALQAKLLRVLETRALERLGSTSTINLDICVIASAQACLDDAVEEGK
FRRDLYFRLNVLTLKLPPLRDQPERILPLFTRFVAASAKELSVPIPDVCPLLQQVLTGHHWPGNIRELKAAAKRHVLGFP
LLGADSQTEEHLACGLKFQLRAIEKALIQQALKRHRNCIDAASLELDIPRRTLYRRIKELSI

Sequences:

>Translated_302_residues
MNLDDEFDDHLDAERVPNLGIVAESISQLGIDVLLSGETGTGKDTIARRIHNMSGRQGRFVPMNCAAIPESLAESELFGV
VSGAYTGADRSRMGYIEAAQGGTLYLDEIDSMPLALQAKLLRVLETRALERLGSTSTINLDICVIASAQACLDDAVEEGK
FRRDLYFRLNVLTLKLPPLRDQPERILPLFTRFVAASAKELSVPIPDVCPLLQQVLTGHHWPGNIRELKAAAKRHVLGFP
LLGADSQTEEHLACGLKFQLRAIEKALIQQALKRHRNCIDAASLELDIPRRTLYRRIKELSI
>Mature_302_residues
MNLDDEFDDHLDAERVPNLGIVAESISQLGIDVLLSGETGTGKDTIARRIHNMSGRQGRFVPMNCAAIPESLAESELFGV
VSGAYTGADRSRMGYIEAAQGGTLYLDEIDSMPLALQAKLLRVLETRALERLGSTSTINLDICVIASAQACLDDAVEEGK
FRRDLYFRLNVLTLKLPPLRDQPERILPLFTRFVAASAKELSVPIPDVCPLLQQVLTGHHWPGNIRELKAAAKRHVLGFP
LLGADSQTEEHLACGLKFQLRAIEKALIQQALKRHRNCIDAASLELDIPRRTLYRRIKELSI

Specific function: Regulates the activation of the sigma factor hrpL which itself induces the expression of hprD as well as other hrp loci which are involved in plant pathogenicity, hrmA and avr genes. Probably interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=288, Percent_Identity=43.0555555555556, Blast_Score=207, Evalue=6e-55,
Organism=Escherichia coli, GI1789233, Length=295, Percent_Identity=41.6949152542373, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI87082117, Length=220, Percent_Identity=50.4545454545455, Blast_Score=190, Evalue=1e-49,
Organism=Escherichia coli, GI1789087, Length=238, Percent_Identity=44.9579831932773, Blast_Score=189, Evalue=1e-49,
Organism=Escherichia coli, GI1786524, Length=286, Percent_Identity=40.5594405594406, Blast_Score=185, Evalue=4e-48,
Organism=Escherichia coli, GI1787583, Length=213, Percent_Identity=45.5399061032864, Blast_Score=181, Evalue=7e-47,
Organism=Escherichia coli, GI1790299, Length=312, Percent_Identity=37.8205128205128, Blast_Score=177, Evalue=7e-46,
Organism=Escherichia coli, GI1790437, Length=278, Percent_Identity=37.7697841726619, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI1788905, Length=209, Percent_Identity=43.5406698564593, Blast_Score=171, Evalue=5e-44,
Organism=Escherichia coli, GI87081872, Length=236, Percent_Identity=41.1016949152542, Blast_Score=168, Evalue=4e-43,
Organism=Escherichia coli, GI87082152, Length=215, Percent_Identity=42.3255813953488, Blast_Score=160, Evalue=9e-41,
Organism=Escherichia coli, GI1789828, Length=238, Percent_Identity=36.5546218487395, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI87081858, Length=286, Percent_Identity=30.0699300699301, Blast_Score=105, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 33400; Mature: 33400

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLDDEFDDHLDAERVPNLGIVAESISQLGIDVLLSGETGTGKDTIARRIHNMSGRQGRF
CCCCCCHHHCCCHHHCCCCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCE
VPMNCAAIPESLAESELFGVVSGAYTGADRSRMGYIEAAQGGTLYLDEIDSMPLALQAKL
EECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCEEEEECCCCCCHHHHHHH
LRVLETRALERLGSTSTINLDICVIASAQACLDDAVEEGKFRRDLYFRLNVLTLKLPPLR
HHHHHHHHHHHCCCCCEEEEEEEEECCHHHHHHHHHHHCCHHHHHEEEEEEEEEECCCCC
DQPERILPLFTRFVAASAKELSVPIPDVCPLLQQVLTGHHWPGNIRELKAAAKRHVLGFP
CCHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCC
LLGADSQTEEHLACGLKFQLRAIEKALIQQALKRHRNCIDAASLELDIPRRTLYRRIKEL
EECCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEECCCHHHHHHHHHHH
SI
CC
>Mature Secondary Structure
MNLDDEFDDHLDAERVPNLGIVAESISQLGIDVLLSGETGTGKDTIARRIHNMSGRQGRF
CCCCCCHHHCCCHHHCCCCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCE
VPMNCAAIPESLAESELFGVVSGAYTGADRSRMGYIEAAQGGTLYLDEIDSMPLALQAKL
EECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCEEEEECCCCCCHHHHHHH
LRVLETRALERLGSTSTINLDICVIASAQACLDDAVEEGKFRRDLYFRLNVLTLKLPPLR
HHHHHHHHHHHCCCCCEEEEEEEEECCHHHHHHHHHHHCCHHHHHEEEEEEEEEECCCCC
DQPERILPLFTRFVAASAKELSVPIPDVCPLLQQVLTGHHWPGNIRELKAAAKRHVLGFP
CCHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCC
LLGADSQTEEHLACGLKFQLRAIEKALIQQALKRHRNCIDAASLELDIPRRTLYRRIKEL
EECCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEECCCHHHHHHHHHHH
SI
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8106313 [H]