Click here to switch to the map view.

The map label for this gene is pelA [H]

Identifier: 66044435

GI number: 66044435

Start: 1338293

End: 1339618

Strand: Reverse

Name: pelA [H]

Synonym: Psyr_1184

Alternate gene names: 66044435

Gene position: 1339618-1338293 (Counterclockwise)

Preceding gene: 66044439

Following gene: 66044433

Centisome position: 21.98

GC content: 59.13

Gene sequence:

>1326_bases
ATGAGCATCGGTATCAACTCATCCACCTCCTACCAACCCGCTTCAACCCAGCTGGACTTCTCGGCGCTGAGCGGCAAGAG
TCCGCAGACCAATACGTTCAGCGATGAGAGCACTGCACAGGGCGTCGACCCGAGTGCCCTGCTGTTCGACACTGCCAGAC
AGAAAGACGTCAGCTTCGGACAGCCCGACAATACGGTTCAAAACCCCACCGACAGCAGCGCTGCAACTGACCCTCAATCC
AACGTCGTCAAGCTGCTCAGTGCACTCGTCACATCGCTGCTGCAAATGCTCATGAACCTCAATAAAAAACAGGACACGGG
TCAGGACTCCAACGAGTGGCAAGACCCGTTTCAAAACGAGGGAGGACTCGGTACGCCATCGGCCGAAGGCAGTGACGGTG
GTACTCAGGAGGCTTCAGGCGGTGACGAGGGCGGCGGTACAACGGCGGCGACGGGTGGTGACGGTGGTGGCGGTACATCC
CCGACCACTGAAGGTGACGGCGGCGGTACATCTCCAACCGCTGAAGGTGACGGCGGCGGTAGTTATGTGTCGACTGGCGC
AGACGGCAGCGGCGCGCCTTCGACCGAGGACGGAACCGGCGGAGGCGGTGGCAGTGATGGCGTGACGCCTCAAGTCACCC
CGCAACTGGCCAATCCCGGCCGCAACTCGGGCAACGGCACGGTTTCGGATACAACGGGCTCTCTTGAGCAATCCGGCGAA
GTCAATGTCGTGAAAGACACCATCAAAGTCGGTGCTGGCCAGGTGTTTGACGGCCACGGTGCAACCTTTACCGCCGACAA
GTCGATGGGCACCGGCGACCAGGACGAACATCAGAAGCCCCTGTTCGAACTGGCCGAAGGCGCTGTGCTGAAAAACGTGA
ACCTGGGCGAGAACGAAGCCGACGGCATCCACGTGAATGCCAAAAACTCGGAGCAAGTCACCATTGATAACGTGCACGCC
CAGAACGTCGGTGAAGACATGATCACGGTCAAGGGCGAAGGCGGCGCAAAGGTCACCAACCTGAACATCACTAACAGCAG
CGCCAACGGTGCCGATGACAAGGTCATTCAGCTCAATGCCGATACCCACCTGAAAGTCGACGGTTTCAAGGCCACCGATT
TCGGTACGCTGGTCCGCACCAATGGTGGCAAGCAGTTTGATGACATGAGTGTCGAGCTCAATGGCGTGGACGCGACCCAT
GGCAAGTTCGCACTCGTCAAAAGCGACAGTGACGATCTGAAACTGGCCACTGGCGACATCGCCATGACCGACGTCAAGCA
CGCCTATGACAAGACCAAGGCGTCTACCCAGCACACCGAGCTCTGA

Upstream 100 bases:

>100_bases
CTCATCGACCTATAGAACCCGACAAGGACGCGGCGAGCAACGCCGGTAAATCCGGAAGACGACGCTGGTTCATATGTCCA
TCCCATCGAGGTAAAGCACC

Downstream 100 bases:

>100_bases
TACGACGCATACATGAGAAACAAGCAAAGGGGCGCACTCCGTGGAAGGCGCCCCTTTGCTTTACCGGCTATGGGTCGAAA
CGCTTATGAGCGCCCTGGCT

Product: type III helper protein HrpW1

Products: NA

Alternate protein names: Pectin lyase [H]

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MSIGINSSTSYQPASTQLDFSALSGKSPQTNTFSDESTAQGVDPSALLFDTARQKDVSFGQPDNTVQNPTDSSAATDPQS
NVVKLLSALVTSLLQMLMNLNKKQDTGQDSNEWQDPFQNEGGLGTPSAEGSDGGTQEASGGDEGGGTTAATGGDGGGGTS
PTTEGDGGGTSPTAEGDGGGSYVSTGADGSGAPSTEDGTGGGGGSDGVTPQVTPQLANPGRNSGNGTVSDTTGSLEQSGE
VNVVKDTIKVGAGQVFDGHGATFTADKSMGTGDQDEHQKPLFELAEGAVLKNVNLGENEADGIHVNAKNSEQVTIDNVHA
QNVGEDMITVKGEGGAKVTNLNITNSSANGADDKVIQLNADTHLKVDGFKATDFGTLVRTNGGKQFDDMSVELNGVDATH
GKFALVKSDSDDLKLATGDIAMTDVKHAYDKTKASTQHTEL

Sequences:

>Translated_441_residues
MSIGINSSTSYQPASTQLDFSALSGKSPQTNTFSDESTAQGVDPSALLFDTARQKDVSFGQPDNTVQNPTDSSAATDPQS
NVVKLLSALVTSLLQMLMNLNKKQDTGQDSNEWQDPFQNEGGLGTPSAEGSDGGTQEASGGDEGGGTTAATGGDGGGGTS
PTTEGDGGGTSPTAEGDGGGSYVSTGADGSGAPSTEDGTGGGGGSDGVTPQVTPQLANPGRNSGNGTVSDTTGSLEQSGE
VNVVKDTIKVGAGQVFDGHGATFTADKSMGTGDQDEHQKPLFELAEGAVLKNVNLGENEADGIHVNAKNSEQVTIDNVHA
QNVGEDMITVKGEGGAKVTNLNITNSSANGADDKVIQLNADTHLKVDGFKATDFGTLVRTNGGKQFDDMSVELNGVDATH
GKFALVKSDSDDLKLATGDIAMTDVKHAYDKTKASTQHTEL
>Mature_440_residues
SIGINSSTSYQPASTQLDFSALSGKSPQTNTFSDESTAQGVDPSALLFDTARQKDVSFGQPDNTVQNPTDSSAATDPQSN
VVKLLSALVTSLLQMLMNLNKKQDTGQDSNEWQDPFQNEGGLGTPSAEGSDGGTQEASGGDEGGGTTAATGGDGGGGTSP
TTEGDGGGTSPTAEGDGGGSYVSTGADGSGAPSTEDGTGGGGGSDGVTPQVTPQLANPGRNSGNGTVSDTTGSLEQSGEV
NVVKDTIKVGAGQVFDGHGATFTADKSMGTGDQDEHQKPLFELAEGAVLKNVNLGENEADGIHVNAKNSEQVTIDNVHAQ
NVGEDMITVKGEGGAKVTNLNITNSSANGADDKVIQLNADTHLKVDGFKATDFGTLVRTNGGKQFDDMSVELNGVDATHG
KFALVKSDSDDLKLATGDIAMTDVKHAYDKTKASTQHTEL

Specific function: Catalyzes the depolymerization of both polygalacturonate and pectins of methyl esterification degree from 22 to 89%, with an endo mode of action. In contrast to the majority of pectate lyases, displays high activity on highly methylated pectins. Is not ab

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide lyase 3 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004898
- InterPro:   IPR012334
- InterPro:   IPR011050 [H]

Pfam domain/function: PF03211 Pectate_lyase [H]

EC number: =4.2.2.2; =4.2.2.10 [H]

Molecular weight: Translated: 44714; Mature: 44583

Theoretical pI: Translated: 4.04; Mature: 4.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIGINSSTSYQPASTQLDFSALSGKSPQTNTFSDESTAQGVDPSALLFDTARQKDVSFG
CCCCCCCCCCCCCCCCEEEHHHHCCCCCCCCCCCCCCCCCCCCCHHEEECCHHHCCCCCC
QPDNTVQNPTDSSAATDPQSNVVKLLSALVTSLLQMLMNLNKKQDTGQDSNEWQDPFQNE
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCHHCCC
GGLGTPSAEGSDGGTQEASGGDEGGGTTAATGGDGGGGTSPTTEGDGGGTSPTAEGDGGG
CCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SYVSTGADGSGAPSTEDGTGGGGGSDGVTPQVTPQLANPGRNSGNGTVSDTTGSLEQSGE
CEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCEEECCCCCHHCCCC
VNVVKDTIKVGAGQVFDGHGATFTADKSMGTGDQDEHQKPLFELAEGAVLKNVNLGENEA
EEEEEHHHHHCCCEEECCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCEEECCCCCCCCC
DGIHVNAKNSEQVTIDNVHAQNVGEDMITVKGEGGAKVTNLNITNSSANGADDKVIQLNA
CCEEEECCCCCEEEEECCCCCCCCCCEEEEECCCCCEEEEEEEECCCCCCCCCEEEEECC
DTHLKVDGFKATDFGTLVRTNGGKQFDDMSVELNGVDATHGKFALVKSDSDDLKLATGDI
CCEEEECCEECCCCCEEEECCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCEEEEECCE
AMTDVKHAYDKTKASTQHTEL
EEHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SIGINSSTSYQPASTQLDFSALSGKSPQTNTFSDESTAQGVDPSALLFDTARQKDVSFG
CCCCCCCCCCCCCCCEEEHHHHCCCCCCCCCCCCCCCCCCCCCHHEEECCHHHCCCCCC
QPDNTVQNPTDSSAATDPQSNVVKLLSALVTSLLQMLMNLNKKQDTGQDSNEWQDPFQNE
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCHHCCC
GGLGTPSAEGSDGGTQEASGGDEGGGTTAATGGDGGGGTSPTTEGDGGGTSPTAEGDGGG
CCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SYVSTGADGSGAPSTEDGTGGGGGSDGVTPQVTPQLANPGRNSGNGTVSDTTGSLEQSGE
CEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCEEECCCCCHHCCCC
VNVVKDTIKVGAGQVFDGHGATFTADKSMGTGDQDEHQKPLFELAEGAVLKNVNLGENEA
EEEEEHHHHHCCCEEECCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCEEECCCCCCCCC
DGIHVNAKNSEQVTIDNVHAQNVGEDMITVKGEGGAKVTNLNITNSSANGADDKVIQLNA
CCEEEECCCCCEEEEECCCCCCCCCCEEEEECCCCCEEEEEEEECCCCCCCCCEEEEECC
DTHLKVDGFKATDFGTLVRTNGGKQFDDMSVELNGVDATHGKFALVKSDSDDLKLATGDI
CCEEEECCEECCCCCEEEECCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCEEEEECCE
AMTDVKHAYDKTKASTQHTEL
EEHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10658655 [H]