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The map label for this gene is rutB [H]

Identifier: 66044416

GI number: 66044416

Start: 1318952

End: 1319638

Strand: Reverse

Name: rutB [H]

Synonym: Psyr_1165

Alternate gene names: 66044416

Gene position: 1319638-1318952 (Counterclockwise)

Preceding gene: 66044417

Following gene: 66044415

Centisome position: 21.66

GC content: 63.9

Gene sequence:

>687_bases
ATGAGCGAACGTCATATTGCATCGGCGCCCTACCCCTGGCCCTGGAACGGGCAATTGCATGCGCACAACACGGCCTTGAT
CGTGATCGACATGCAGACCGACTTCTGCGGCGTCGGCGGCTACGTGGACAGCATGGGTTACGATCTGGCGCTGACCCGTG
CGCCCATCGAGCCGATCAAGGCGCTGCTCGCCACAATGCGCCCGCTGGGCTTCACCATCATCCATACCCGCGAAGGTCAT
CGCCCGGACCTCAGTGACTTGCCCGCCAACAAACGCTGGCGCTCGCAGCGCATCGGCGCGGGAATCGGCGACCCCGGTCC
GTGCGGCAAGATTCTGGTGCGTGGCGAGCCGGGCTGGGAAATCATTGACGAGTTGGCACCGCTGCCCGGCGAAATCGTGC
TCGACAAGCCCGGCAAAGGCTCCTTCTGCGCCACCGATCTGGAACTGATTCTGCGCACTCGCGGCATCGACAATCTGATC
CTGACCGGCATCACCACCGACGTCTGCGTGCACACCACCCTGCGCGAAGCCAATGACCGTGGCTTCGAGTGCCTGCTGCT
GGAAGACTGCTGCGGGGCGACCGACCCTGACAACCATGCGGCGGCCCTGAGCATGGTCAAAATGCAGGGTGGCGTGTTCG
GTGCGGTCGGCCATTCCTCGATGCTTCGCGACCTGCTGGGAGCATGA

Upstream 100 bases:

>100_bases
TGGGAAGACGATGTCGTGCATACCGATGGCAGCTCGTGCGGTTTTGCGGCCCCCGAGCGCGACTTCAAACCCACCCCCGG
CAGCTGGAAGGAGTAACGCC

Downstream 100 bases:

>100_bases
ACATGCGCGCCCCGAGCCTTGAGACGATTGGTGCGAGCAAATACTTCGGCAGTTTTTGCGCGCTCGACGAGGTGTCCTTC
AAGGTCCGCGCCGGCACCGT

Product: isochorismatase hydrolase

Products: NA

Alternate protein names: Ureidoacrylate amidohydrolase [H]

Number of amino acids: Translated: 228; Mature: 227

Protein sequence:

>228_residues
MSERHIASAPYPWPWNGQLHAHNTALIVIDMQTDFCGVGGYVDSMGYDLALTRAPIEPIKALLATMRPLGFTIIHTREGH
RPDLSDLPANKRWRSQRIGAGIGDPGPCGKILVRGEPGWEIIDELAPLPGEIVLDKPGKGSFCATDLELILRTRGIDNLI
LTGITTDVCVHTTLREANDRGFECLLLEDCCGATDPDNHAAALSMVKMQGGVFGAVGHSSMLRDLLGA

Sequences:

>Translated_228_residues
MSERHIASAPYPWPWNGQLHAHNTALIVIDMQTDFCGVGGYVDSMGYDLALTRAPIEPIKALLATMRPLGFTIIHTREGH
RPDLSDLPANKRWRSQRIGAGIGDPGPCGKILVRGEPGWEIIDELAPLPGEIVLDKPGKGSFCATDLELILRTRGIDNLI
LTGITTDVCVHTTLREANDRGFECLLLEDCCGATDPDNHAAALSMVKMQGGVFGAVGHSSMLRDLLGA
>Mature_227_residues
SERHIASAPYPWPWNGQLHAHNTALIVIDMQTDFCGVGGYVDSMGYDLALTRAPIEPIKALLATMRPLGFTIIHTREGHR
PDLSDLPANKRWRSQRIGAGIGDPGPCGKILVRGEPGWEIIDELAPLPGEIVLDKPGKGSFCATDLELILRTRGIDNLIL
TGITTDVCVHTTLREANDRGFECLLLEDCCGATDPDNHAAALSMVKMQGGVFGAVGHSSMLRDLLGA

Specific function: In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby rele

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family. RutB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081820, Length=210, Percent_Identity=34.2857142857143, Blast_Score=111, Evalue=4e-26,
Organism=Escherichia coli, GI87081992, Length=189, Percent_Identity=30.1587301587302, Blast_Score=74, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019916
- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: NA

Molecular weight: Translated: 24603; Mature: 24471

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSERHIASAPYPWPWNGQLHAHNTALIVIDMQTDFCGVGGYVDSMGYDLALTRAPIEPIK
CCCCCCCCCCCCCCCCCEEEECCEEEEEEECCCCCCCCCCHHHHCCCEEEEECCCHHHHH
ALLATMRPLGFTIIHTREGHRPDLSDLPANKRWRSQRIGAGIGDPGPCGKILVRGEPGWE
HHHHHHCCCCEEEEEECCCCCCCHHCCCCCCCHHHHHCCCCCCCCCCCCEEEEECCCCHH
IIDELAPLPGEIVLDKPGKGSFCATDLELILRTRGIDNLILTGITTDVCVHTTLREANDR
HHHHHCCCCCEEEEECCCCCCEEHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHCCCC
GFECLLLEDCCGATDPDNHAAALSMVKMQGGVFGAVGHSSMLRDLLGA
CEEEEEEEHHCCCCCCCCHHHHHHHHHHCCCEEEECCHHHHHHHHHCC
>Mature Secondary Structure 
SERHIASAPYPWPWNGQLHAHNTALIVIDMQTDFCGVGGYVDSMGYDLALTRAPIEPIK
CCCCCCCCCCCCCCCCEEEECCEEEEEEECCCCCCCCCCHHHHCCCEEEEECCCHHHHH
ALLATMRPLGFTIIHTREGHRPDLSDLPANKRWRSQRIGAGIGDPGPCGKILVRGEPGWE
HHHHHHCCCCEEEEEECCCCCCCHHCCCCCCCHHHHHCCCCCCCCCCCCEEEEECCCCHH
IIDELAPLPGEIVLDKPGKGSFCATDLELILRTRGIDNLILTGITTDVCVHTTLREANDR
HHHHHCCCCCEEEEECCCCCCEEHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHCCCC
GFECLLLEDCCGATDPDNHAAALSMVKMQGGVFGAVGHSSMLRDLLGA
CEEEEEEEHHCCCCCCCCHHHHHHHHHHCCCEEEECCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA