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The map label for this gene is ilvE [H]

Identifier: 66044399

GI number: 66044399

Start: 1299152

End: 1300171

Strand: Direct

Name: ilvE [H]

Synonym: Psyr_1148

Alternate gene names: 66044399

Gene position: 1299152-1300171 (Clockwise)

Preceding gene: 66044398

Following gene: 66044403

Centisome position: 21.32

GC content: 59.12

Gene sequence:

>1020_bases
ATGAGTCAAGAAAGCATCAACTGGGACACTCTGGGTTTTGATTACATCAAGACCGACAAGCGCTACCTTTCTCATTGGCG
CGACGGTGCCTGGGATCAGGGCTCACTGACCGAGGACAACACGCTGCATATCAGCGAAGGGTCGACCGCGCTGCATTATG
GCCAGCAGTGCTTCGAGGGCCTCAAGGCCTACCGCTGCAAGGATGGCTCGATCAATCTGTTCCGTCCCGATCAGAACGCC
CAGCGCATGCAGCGCAGCTGCTCCCGTCTGTTGATGCCCCACGTCCCGACCGATGTCTTCATTGAAGCCTGCAAGCAGGT
GGTCAAGGCCAACGAACGCTTCATCCCGCCTTACGGTTCTGGCGGCGCGCTGTACCTGCGCCCGTTCGTCATCGGTGTGG
GTGACAACATCGGCGTGCGCACGGCGCCTGAATTCATCTTCTCGATTTTCTGCATCCCGGTGGGCGCCTATTTCAAGGGG
GGCCTGAAGCCGAACAATTTCGTCATCTCCGGCTACGACCGCGCTGCGCCCAACGGTACAGGTGCAGCCAAGGTCGGCGG
TAACTACGCCGCCAGCCTGATGCCCGGTTCCGAAGCCAAGAAGCACAGCTTTGCAGATTGCATCTATCTCGATCCGCAGA
CCCATTCCAAGATCGAGGAAGTGGGCTCGGCCAACTTCTTTGCCATCACCAAGGATGATGTGTTCCTGACGCCCAAGTCG
CCATCGGTGCTGCCGGGTATCACCCGCCTGTCGTTGATCGAACTGGCGAAATCCCGTCTGGGCCTGACGGTCGAAGAGGG
TGATGTGTTCATCGACCAGCTCGGCGAGTTCAAGGAAGCCGGCGCATGCGGTACGGCTGCGGTCATTACTCCGATCGGCG
GGATTTCCTACAAGGACAACCTGCACGTGTTCTACAGCCAGACCGAAGTCGGTCCTGTGACTCAGCGTCTGTACAAGGAG
CTGACCGGCGTTCAGTCCGGTGACATCGAGGCTCCAGCCGGCTGGATCGTCAAGGTGTGA

Upstream 100 bases:

>100_bases
TCGGTTTGCATTTATTTCGAGCCTCATGGCCTGAATGATCTGGCAGAATAAGGCATTGGCCTGACTCGACCAGGCAGTCG
CTAAAATGATAGAGGGCGTT

Downstream 100 bases:

>100_bases
TGAGCTGACCCGGATCGTTCCAGCGTCGAACGCTGGAACGATCATTGACCACAGGTTCAGGCTTCAGGTGTTTTTTCTTC
CTCAAGCGGCTCTTTCGCCG

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MSQESINWDTLGFDYIKTDKRYLSHWRDGAWDQGSLTEDNTLHISEGSTALHYGQQCFEGLKAYRCKDGSINLFRPDQNA
QRMQRSCSRLLMPHVPTDVFIEACKQVVKANERFIPPYGSGGALYLRPFVIGVGDNIGVRTAPEFIFSIFCIPVGAYFKG
GLKPNNFVISGYDRAAPNGTGAAKVGGNYAASLMPGSEAKKHSFADCIYLDPQTHSKIEEVGSANFFAITKDDVFLTPKS
PSVLPGITRLSLIELAKSRLGLTVEEGDVFIDQLGEFKEAGACGTAAVITPIGGISYKDNLHVFYSQTEVGPVTQRLYKE
LTGVQSGDIEAPAGWIVKV

Sequences:

>Translated_339_residues
MSQESINWDTLGFDYIKTDKRYLSHWRDGAWDQGSLTEDNTLHISEGSTALHYGQQCFEGLKAYRCKDGSINLFRPDQNA
QRMQRSCSRLLMPHVPTDVFIEACKQVVKANERFIPPYGSGGALYLRPFVIGVGDNIGVRTAPEFIFSIFCIPVGAYFKG
GLKPNNFVISGYDRAAPNGTGAAKVGGNYAASLMPGSEAKKHSFADCIYLDPQTHSKIEEVGSANFFAITKDDVFLTPKS
PSVLPGITRLSLIELAKSRLGLTVEEGDVFIDQLGEFKEAGACGTAAVITPIGGISYKDNLHVFYSQTEVGPVTQRLYKE
LTGVQSGDIEAPAGWIVKV
>Mature_338_residues
SQESINWDTLGFDYIKTDKRYLSHWRDGAWDQGSLTEDNTLHISEGSTALHYGQQCFEGLKAYRCKDGSINLFRPDQNAQ
RMQRSCSRLLMPHVPTDVFIEACKQVVKANERFIPPYGSGGALYLRPFVIGVGDNIGVRTAPEFIFSIFCIPVGAYFKGG
LKPNNFVISGYDRAAPNGTGAAKVGGNYAASLMPGSEAKKHSFADCIYLDPQTHSKIEEVGSANFFAITKDDVFLTPKSP
SVLPGITRLSLIELAKSRLGLTVEEGDVFIDQLGEFKEAGACGTAAVITPIGGISYKDNLHVFYSQTEVGPVTQRLYKEL
TGVQSGDIEAPAGWIVKV

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI50658084, Length=334, Percent_Identity=32.6347305389222, Blast_Score=165, Evalue=7e-41,
Organism=Homo sapiens, GI38176287, Length=339, Percent_Identity=32.1533923303835, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI296010906, Length=339, Percent_Identity=32.1533923303835, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI296010904, Length=339, Percent_Identity=32.1533923303835, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI258614015, Length=287, Percent_Identity=33.4494773519164, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI296010902, Length=339, Percent_Identity=26.8436578171091, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI296010900, Length=339, Percent_Identity=26.8436578171091, Blast_Score=108, Evalue=6e-24,
Organism=Escherichia coli, GI48994963, Length=322, Percent_Identity=33.2298136645963, Blast_Score=142, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17568601, Length=347, Percent_Identity=30.2593659942363, Blast_Score=153, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17565728, Length=336, Percent_Identity=29.4642857142857, Blast_Score=105, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6322608, Length=332, Percent_Identity=30.421686746988, Blast_Score=164, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6322002, Length=319, Percent_Identity=30.4075235109718, Blast_Score=155, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24641779, Length=348, Percent_Identity=29.0229885057471, Blast_Score=156, Evalue=2e-38,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 37023; Mature: 36892

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQESINWDTLGFDYIKTDKRYLSHWRDGAWDQGSLTEDNTLHISEGSTALHYGQQCFEG
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHH
LKAYRCKDGSINLFRPDQNAQRMQRSCSRLLMPHVPTDVFIEACKQVVKANERFIPPYGS
HHHEECCCCCEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
GGALYLRPFVIGVGDNIGVRTAPEFIFSIFCIPVGAYFKGGLKPNNFVISGYDRAAPNGT
CCEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCC
GAAKVGGNYAASLMPGSEAKKHSFADCIYLDPQTHSKIEEVGSANFFAITKDDVFLTPKS
CCEECCCCEEEECCCCCCCCCCCCCCEEEECCCHHHHHHHHCCCCEEEEECCCEEECCCC
PSVLPGITRLSLIELAKSRLGLTVEEGDVFIDQLGEFKEAGACGTAAVITPIGGISYKDN
CCCCCCHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCC
LHVFYSQTEVGPVTQRLYKELTGVQSGDIEAPAGWIVKV
EEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEC
>Mature Secondary Structure 
SQESINWDTLGFDYIKTDKRYLSHWRDGAWDQGSLTEDNTLHISEGSTALHYGQQCFEG
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHH
LKAYRCKDGSINLFRPDQNAQRMQRSCSRLLMPHVPTDVFIEACKQVVKANERFIPPYGS
HHHEECCCCCEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
GGALYLRPFVIGVGDNIGVRTAPEFIFSIFCIPVGAYFKGGLKPNNFVISGYDRAAPNGT
CCEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCC
GAAKVGGNYAASLMPGSEAKKHSFADCIYLDPQTHSKIEEVGSANFFAITKDDVFLTPKS
CCEECCCCEEEECCCCCCCCCCCCCCEEEECCCHHHHHHHHCCCCEEEEECCCEEECCCC
PSVLPGITRLSLIELAKSRLGLTVEEGDVFIDQLGEFKEAGACGTAAVITPIGGISYKDN
CCCCCCHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCC
LHVFYSQTEVGPVTQRLYKELTGVQSGDIEAPAGWIVKV
EEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]