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The map label for this gene is rlmF [H]

Identifier: 66044338

GI number: 66044338

Start: 1226571

End: 1227557

Strand: Reverse

Name: rlmF [H]

Synonym: Psyr_1085

Alternate gene names: 66044338

Gene position: 1227557-1226571 (Counterclockwise)

Preceding gene: 66044339

Following gene: 66044336

Centisome position: 20.14

GC content: 62.41

Gene sequence:

>987_bases
ATGACCGATACCCGCAAACCACCGCGCAAGAAGCCTCAGCGGCCAGCCAAACCTGCTGCGCCGCGTGAAAAGGCCACCTT
GCACCCGCGCAATCGGCATCAGGGCCAGTACGACTTTGCGAAGCTGATCAAATCCAGCCCCGAGCTGGCAGCGTTCGTGA
TCCTCAATCCTTACGGCAAGGAAAGCATCGACTTCGCCAATCCGCAGGCGGTACGGGTGTTCAATCGCGCGCTGCTCAAG
GCGTTCTATGGCATCGCCCATTGGGACATTCCAGCCGATTACCTGTGCCCGCCAATTCCCGGGCGTGCGGACTACCTGCA
CTTCCTCGCCGACCTGCTGGCCGAAGACAATGAAGGCGTGATACCACGCGGCGCATCCATCAAGGCGCTGGACATCGGCA
CCGGCGCCAATTGCATCTACCCGTTGTTGGGGCATAGCGACTATGGCTGGCAGTTCGTGGGTTCGGACATCGACAGCACC
GCCATCGCCGCAGCCACCACGATTGTGAAGGCCAACGGCCTCAGCAAGGCCATCAGCGTGCGCCAGCAGGCCAATCGCAG
GCAGATCCTGCTGGGGCTGCTCGACAGCAGCGAACGCTTCCACGTCAGCCTCTGCAACCCGCCCTTCCATGCCTCGCTGG
AAGAAGCGCAGCGTGGCAGCCAGCGCAAGTGGCGGGCGCTGGGCAAGGCTGATCCCAAGCGCAAGCTGCCGGTGCTCAAT
TTCGGTGGACAGTCGCAGGAGTTGTGGTGCGACGGCGGCGAGATCGGCTTCATAACGCAGTTGATCCAGGAAAGCGCCTT
GCTGCCGAGCCAGGTGGCGTGGTTCAGCACACTGGTGTCCAAGGCCTCCAACCTGCCGCCGATCCACAGCGCCCTGAAAA
AAGCCGGCGCGCTGGAAGTGAAGGTAGTGGAAATGGGCCAGGGCCAGAAGCAAAGCCGTTTTGTGGCCTGGACCTTCCTC
GACAAGGCACAGCGCGCACCAGGCTGA

Upstream 100 bases:

>100_bases
TACTGACTTATTCGCGAGCAAGGCCTGTCACGCCAAAGGCGACGCTGCGCTTGCTCGCGAATAAGCCAGGCAACTCAACC
TCACGGCTGACATCGAATCC

Downstream 100 bases:

>100_bases
CTCGACTATCGTGAACAAGCGCCTAATCTCGACTATCGTGCGACGCCGCGTCGGCAGGCCGTAATCTCGACTATCGTGCG
ACTCCACAGCGACATGCCTT

Product: putative SAM-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA mA1618 methyltransferase; rRNA adenine N-6-methyltransferase [H]

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MTDTRKPPRKKPQRPAKPAAPREKATLHPRNRHQGQYDFAKLIKSSPELAAFVILNPYGKESIDFANPQAVRVFNRALLK
AFYGIAHWDIPADYLCPPIPGRADYLHFLADLLAEDNEGVIPRGASIKALDIGTGANCIYPLLGHSDYGWQFVGSDIDST
AIAAATTIVKANGLSKAISVRQQANRRQILLGLLDSSERFHVSLCNPPFHASLEEAQRGSQRKWRALGKADPKRKLPVLN
FGGQSQELWCDGGEIGFITQLIQESALLPSQVAWFSTLVSKASNLPPIHSALKKAGALEVKVVEMGQGQKQSRFVAWTFL
DKAQRAPG

Sequences:

>Translated_328_residues
MTDTRKPPRKKPQRPAKPAAPREKATLHPRNRHQGQYDFAKLIKSSPELAAFVILNPYGKESIDFANPQAVRVFNRALLK
AFYGIAHWDIPADYLCPPIPGRADYLHFLADLLAEDNEGVIPRGASIKALDIGTGANCIYPLLGHSDYGWQFVGSDIDST
AIAAATTIVKANGLSKAISVRQQANRRQILLGLLDSSERFHVSLCNPPFHASLEEAQRGSQRKWRALGKADPKRKLPVLN
FGGQSQELWCDGGEIGFITQLIQESALLPSQVAWFSTLVSKASNLPPIHSALKKAGALEVKVVEMGQGQKQSRFVAWTFL
DKAQRAPG
>Mature_327_residues
TDTRKPPRKKPQRPAKPAAPREKATLHPRNRHQGQYDFAKLIKSSPELAAFVILNPYGKESIDFANPQAVRVFNRALLKA
FYGIAHWDIPADYLCPPIPGRADYLHFLADLLAEDNEGVIPRGASIKALDIGTGANCIYPLLGHSDYGWQFVGSDIDSTA
IAAATTIVKANGLSKAISVRQQANRRQILLGLLDSSERFHVSLCNPPFHASLEEAQRGSQRKWRALGKADPKRKLPVLNF
GGQSQELWCDGGEIGFITQLIQESALLPSQVAWFSTLVSKASNLPPIHSALKKAGALEVKVVEMGQGQKQSRFVAWTFLD
KAQRAPG

Specific function: Specifically methylates the adenine in position 1618 of 23S rRNA [H]

COG id: COG3129

COG function: function code R; Predicted SAM-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. METT10D/rlmF family [H]

Homologues:

Organism=Homo sapiens, GI122114654, Length=301, Percent_Identity=32.5581395348837, Blast_Score=170, Evalue=2e-42,
Organism=Escherichia coli, GI87081786, Length=304, Percent_Identity=53.6184210526316, Blast_Score=340, Evalue=6e-95,
Organism=Caenorhabditis elegans, GI32564931, Length=307, Percent_Identity=27.6872964169381, Blast_Score=138, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI32564933, Length=307, Percent_Identity=25.7328990228013, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI281363415, Length=303, Percent_Identity=30.03300330033, Blast_Score=124, Evalue=8e-29,
Organism=Drosophila melanogaster, GI19922302, Length=303, Percent_Identity=30.03300330033, Blast_Score=124, Evalue=8e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016909
- InterPro:   IPR010286 [H]

Pfam domain/function: PF05971 Methyltransf_10 [H]

EC number: =2.1.1.181 [H]

Molecular weight: Translated: 36034; Mature: 35902

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTRKPPRKKPQRPAKPAAPREKATLHPRNRHQGQYDFAKLIKSSPELAAFVILNPYGK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEECCCCC
ESIDFANPQAVRVFNRALLKAFYGIAHWDIPADYLCPPIPGRADYLHFLADLLAEDNEGV
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHHHHHCCCCCC
IPRGASIKALDIGTGANCIYPLLGHSDYGWQFVGSDIDSTAIAAATTIVKANGLSKAISV
CCCCCCEEEEECCCCCCHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHCCHHHHHHH
RQQANRRQILLGLLDSSERFHVSLCNPPFHASLEEAQRGSQRKWRALGKADPKRKLPVLN
HHHCCCCEEEEEEECCCCEEEEEECCCCCCCCHHHHHCCCHHHHHHHCCCCCCCCCCEEE
FGGQSQELWCDGGEIGFITQLIQESALLPSQVAWFSTLVSKASNLPPIHSALKKAGALEV
CCCCCCEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEE
KVVEMGQGQKQSRFVAWTFLDKAQRAPG
EEEECCCCCCCCEEEEEEEHHHHHCCCC
>Mature Secondary Structure 
TDTRKPPRKKPQRPAKPAAPREKATLHPRNRHQGQYDFAKLIKSSPELAAFVILNPYGK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEECCCCC
ESIDFANPQAVRVFNRALLKAFYGIAHWDIPADYLCPPIPGRADYLHFLADLLAEDNEGV
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHHHHHCCCCCC
IPRGASIKALDIGTGANCIYPLLGHSDYGWQFVGSDIDSTAIAAATTIVKANGLSKAISV
CCCCCCEEEEECCCCCCHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHCCHHHHHHH
RQQANRRQILLGLLDSSERFHVSLCNPPFHASLEEAQRGSQRKWRALGKADPKRKLPVLN
HHHCCCCEEEEEEECCCCEEEEEECCCCCCCCHHHHHCCCHHHHHHHCCCCCCCCCCEEE
FGGQSQELWCDGGEIGFITQLIQESALLPSQVAWFSTLVSKASNLPPIHSALKKAGALEV
CCCCCCEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEE
KVVEMGQGQKQSRFVAWTFLDKAQRAPG
EEEECCCCCCCCEEEEEEEHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA