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The map label for this gene is fliY [H]

Identifier: 66044227

GI number: 66044227

Start: 1106447

End: 1107268

Strand: Reverse

Name: fliY [H]

Synonym: Psyr_0974

Alternate gene names: 66044227

Gene position: 1107268-1106447 (Counterclockwise)

Preceding gene: 66044229

Following gene: 66044226

Centisome position: 18.17

GC content: 58.76

Gene sequence:

>822_bases
GTGAATTATCGCGCTCTGCTGGGCATCGGCCTGGTCACTCTGGCAGCCTCCACGCAAGTTCTGGCCGGCGCCACGCTGGA
TCGCGTGCAGAAAAACAAAGAGCTGGTCAACGTCCTCATGGAAAGTTACCCGCCCTTCTCTTTCCTGAATGACAAGAATG
AACTGGACGGCTTCGATGTCGACGTCGCCAAGGCCGTGGCGCAGAAACTGGGCGTCAAGCTGCGCCTCGAAACACCGTCT
TGGGACGTGATCGCCGCGGGCCACTGGAGCGGACGTTATGACATCTGCGTCTGCTCGATGACACCGAGCAAGGCCCGCGC
CGAAGTGTTCAACTTCCCGGTCGAGTACTACGCCTCGCCTGCAGTGATCGTGGTCAACGCCTCGGATGATCGTATCCATT
CGGCCGAGGACCTGAGCGGCAAGAAAGTCGGCCTGACCAGCGCCTCCAGCTACGAAAGTTACCTGAACAAGAACCTGGTC
ATCGATGGCGCCGAAGACAAGCCCCTGCAGTACCCGTTCGAAGACGTACAGATCGCCCCGTACGACAACGACAACGTGGC
CTTTCAAGACCTTGGGCTGGGTGCTGGCAAGCGGCTGGACGCGATACTGACCAATCTGGTGACCGCAAAACCGCGTCTGG
ATCAGGACAAGCGCTTCAAACTGGCGGGCGAGCCGCTGTATGAAGAACCCAACTCCGTGGCCATCGAAAAAGACGATCCG
GAATGGGACGCCAAGGTTCGCGAAGTCTTCGCCGAACTCAAGAGCGACGGCACCCTGAGCAAGCTGTCGCAAAAGTGGAT
CGGTGCTGACATCAGCAAATGA

Upstream 100 bases:

>100_bases
CTTATATGTATATGAAAAAAACTCATTATGAGTCAGTCTTGTATAACGTATTTAATACGGCCCTTTCATCAAACTACAAA
CACTTTTTCAGGGGATATCT

Downstream 100 bases:

>100_bases
CTTCCTTTCAGCCCCAGCGCCCACCCGAGCAGGCCGAGCAGGCCGAGCAGAGCCTGCTCAAGCGAGTTTTCGGCTTCCGT
ACGCGGCTTTACCTGACCTG

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: CBP; Protein fliY; Sulfate starvation-induced protein 7; SSI7 [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MNYRALLGIGLVTLAASTQVLAGATLDRVQKNKELVNVLMESYPPFSFLNDKNELDGFDVDVAKAVAQKLGVKLRLETPS
WDVIAAGHWSGRYDICVCSMTPSKARAEVFNFPVEYYASPAVIVVNASDDRIHSAEDLSGKKVGLTSASSYESYLNKNLV
IDGAEDKPLQYPFEDVQIAPYDNDNVAFQDLGLGAGKRLDAILTNLVTAKPRLDQDKRFKLAGEPLYEEPNSVAIEKDDP
EWDAKVREVFAELKSDGTLSKLSQKWIGADISK

Sequences:

>Translated_273_residues
MNYRALLGIGLVTLAASTQVLAGATLDRVQKNKELVNVLMESYPPFSFLNDKNELDGFDVDVAKAVAQKLGVKLRLETPS
WDVIAAGHWSGRYDICVCSMTPSKARAEVFNFPVEYYASPAVIVVNASDDRIHSAEDLSGKKVGLTSASSYESYLNKNLV
IDGAEDKPLQYPFEDVQIAPYDNDNVAFQDLGLGAGKRLDAILTNLVTAKPRLDQDKRFKLAGEPLYEEPNSVAIEKDDP
EWDAKVREVFAELKSDGTLSKLSQKWIGADISK
>Mature_273_residues
MNYRALLGIGLVTLAASTQVLAGATLDRVQKNKELVNVLMESYPPFSFLNDKNELDGFDVDVAKAVAQKLGVKLRLETPS
WDVIAAGHWSGRYDICVCSMTPSKARAEVFNFPVEYYASPAVIVVNASDDRIHSAEDLSGKKVGLTSASSYESYLNKNLV
IDGAEDKPLQYPFEDVQIAPYDNDNVAFQDLGLGAGKRLDAILTNLVTAKPRLDQDKRFKLAGEPLYEEPNSVAIEKDDP
EWDAKVREVFAELKSDGTLSKLSQKWIGADISK

Specific function: Part of a binding-protein-dependent transport system for cystine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=281, Percent_Identity=32.7402135231317, Blast_Score=139, Evalue=1e-34,
Organism=Escherichia coli, GI1787085, Length=231, Percent_Identity=25.5411255411255, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788648, Length=238, Percent_Identity=24.7899159663866, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788649, Length=234, Percent_Identity=24.7863247863248, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1787031, Length=262, Percent_Identity=25.5725190839695, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787088, Length=233, Percent_Identity=22.7467811158798, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 30035; Mature: 30035

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYRALLGIGLVTLAASTQVLAGATLDRVQKNKELVNVLMESYPPFSFLNDKNELDGFDV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCH
DVAKAVAQKLGVKLRLETPSWDVIAAGHWSGRYDICVCSMTPSKARAEVFNFPVEYYASP
HHHHHHHHHCCCEEEEECCCCCEEEECCCCCCEEEEEEECCCCHHHHHHHCCCHHHCCCC
AVIVVNASDDRIHSAEDLSGKKVGLTSASSYESYLNKNLVIDGAEDKPLQYPFEDVQIAP
EEEEEECCCCCCCCHHCCCCCEEECCCCHHHHHHCCCCEEEECCCCCCCCCCCCCCEECC
YDNDNVAFQDLGLGAGKRLDAILTNLVTAKPRLDQDKRFKLAGEPLYEEPNSVAIEKDDP
CCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCEEEECCCC
EWDAKVREVFAELKSDGTLSKLSQKWIGADISK
CHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNYRALLGIGLVTLAASTQVLAGATLDRVQKNKELVNVLMESYPPFSFLNDKNELDGFDV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCH
DVAKAVAQKLGVKLRLETPSWDVIAAGHWSGRYDICVCSMTPSKARAEVFNFPVEYYASP
HHHHHHHHHCCCEEEEECCCCCEEEECCCCCCEEEEEEECCCCHHHHHHHCCCHHHCCCC
AVIVVNASDDRIHSAEDLSGKKVGLTSASSYESYLNKNLVIDGAEDKPLQYPFEDVQIAP
EEEEEECCCCCCCCHHCCCCCEEECCCCHHHHHHCCCCEEEECCCCCCCCCCCCCCEECC
YDNDNVAFQDLGLGAGKRLDAILTNLVTAKPRLDQDKRFKLAGEPLYEEPNSVAIEKDDP
CCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCEEEECCCC
EWDAKVREVFAELKSDGTLSKLSQKWIGADISK
CHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]