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The map label for this gene is tilS/hprT [H]

Identifier: 66044223

GI number: 66044223

Start: 1103827

End: 1104384

Strand: Direct

Name: tilS/hprT [H]

Synonym: Psyr_0970

Alternate gene names: 66044223

Gene position: 1103827-1104384 (Clockwise)

Preceding gene: 66044220

Following gene: 66044224

Centisome position: 18.11

GC content: 58.96

Gene sequence:

>558_bases
ATGTCCGCTGATCTCGAGCATATCCGTCAAGTCATGCGCGAAGCTGACTGCCTGTACACCGAGGCCGAAGTGGATGCGGC
CATTGCCCGCGTCGGCGCGCAGATCAATGCCGAACTGGCCGAGCGCAATCCTGTCGTGTTCTGCGTCATGAATGGCGGCC
TGATTTTCTCCGGCAAGCTGCTCACCCATCTGAACTTCCCGCTGGAAGCGTCCTACCTGCACGCGACGCGTTATCGCAAC
GAAACCACCGGCGGCGATCTGTTCTGGAAAGCCAAGCCGGAAGTCTCGTTCATGGACCGTGACGTGCTGATCATCGACGA
CATCCTCGATGAAGGCCACACCCTGGGCGCCATCATCGACTTCTGTAAACACGCCGGTGCCCGCGCTGTGCACACGGCCG
TGCTGATCGACAAGGACCACGACCGCAAGGCTCGCCCGGACCTGAAAGCCGATTACGTCGGCCTGCCGTGCATCGACCGT
TATATCTTCGGCTTCGGTATGGACTACAAAGGTTACTGGCGTAATGCTGCCGGTATCTATGCGGTCAAAGGGATGTAA

Upstream 100 bases:

>100_bases
TGCAAGACACTCGCAACGTTAGTCCAGAATCACTTGCTCTGGCTTGAACGGATGCGTACCTTTGCCCGCTTTTCTCAAAC
TGCCTTACCTGGAGAGCGTC

Downstream 100 bases:

>100_bases
GGCATTCTGACGACCGCTTCTGCGAACAGGGGCGGTCGTCGATTGGCAATTATATGGAAACCCTCAATGAAGCTACTGAT
TCCCGGCGCCGTGGCGTTGG

Product: hypoxanthine-guanine phosphoribosyltransferase

Products: NA

Alternate protein names: tRNA(Ile)-lysidine synthase; tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase; Hypoxanthine-guanine phosphoribosyltransferase; HGPRT; HGPRTase [H]

Number of amino acids: Translated: 185; Mature: 184

Protein sequence:

>185_residues
MSADLEHIRQVMREADCLYTEAEVDAAIARVGAQINAELAERNPVVFCVMNGGLIFSGKLLTHLNFPLEASYLHATRYRN
ETTGGDLFWKAKPEVSFMDRDVLIIDDILDEGHTLGAIIDFCKHAGARAVHTAVLIDKDHDRKARPDLKADYVGLPCIDR
YIFGFGMDYKGYWRNAAGIYAVKGM

Sequences:

>Translated_185_residues
MSADLEHIRQVMREADCLYTEAEVDAAIARVGAQINAELAERNPVVFCVMNGGLIFSGKLLTHLNFPLEASYLHATRYRN
ETTGGDLFWKAKPEVSFMDRDVLIIDDILDEGHTLGAIIDFCKHAGARAVHTAVLIDKDHDRKARPDLKADYVGLPCIDR
YIFGFGMDYKGYWRNAAGIYAVKGM
>Mature_184_residues
SADLEHIRQVMREADCLYTEAEVDAAIARVGAQINAELAERNPVVFCVMNGGLIFSGKLLTHLNFPLEASYLHATRYRNE
TTGGDLFWKAKPEVSFMDRDVLIIDDILDEGHTLGAIIDFCKHAGARAVHTAVLIDKDHDRKARPDLKADYVGLPCIDRY
IFGFGMDYKGYWRNAAGIYAVKGM

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0634

COG function: function code F; Hypoxanthine-guanine phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4504483, Length=156, Percent_Identity=25, Blast_Score=69, Evalue=3e-12,
Organism=Escherichia coli, GI87081689, Length=164, Percent_Identity=23.780487804878, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005904
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR000836
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF00156 Pribosyltran; PF11734 TilS_C [H]

EC number: =2.4.2.8 [H]

Molecular weight: Translated: 20682; Mature: 20551

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS00213 LIPOCALIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSADLEHIRQVMREADCLYTEAEVDAAIARVGAQINAELAERNPVVFCVMNGGLIFSGKL
CCCCHHHHHHHHHHHHHEEEHHHHHHHHHHHCCHHCHHHHCCCCEEEEEECCCEEEECEE
LTHLNFPLEASYLHATRYRNETTGGDLFWKAKPEVSFMDRDVLIIDDILDEGHTLGAIID
EEEECCCCCHHHHHHHHCCCCCCCCCEEEEECCCCEECCCCEEEEEHHHCCCCHHHHHHH
FCKHAGARAVHTAVLIDKDHDRKARPDLKADYVGLPCIDRYIFGFGMDYKGYWRNAAGIY
HHHHCCCCCEEEEEEEECCCCCCCCCCCCCCEECCCHHHHHHHHCCCCCCCCCCCCCCEE
AVKGM
EEECC
>Mature Secondary Structure 
SADLEHIRQVMREADCLYTEAEVDAAIARVGAQINAELAERNPVVFCVMNGGLIFSGKL
CCCHHHHHHHHHHHHHEEEHHHHHHHHHHHCCHHCHHHHCCCCEEEEEECCCEEEECEE
LTHLNFPLEASYLHATRYRNETTGGDLFWKAKPEVSFMDRDVLIIDDILDEGHTLGAIID
EEEECCCCCHHHHHHHHCCCCCCCCCEEEEECCCCEECCCCEEEEEHHHCCCCHHHHHHH
FCKHAGARAVHTAVLIDKDHDRKARPDLKADYVGLPCIDRYIFGFGMDYKGYWRNAAGIY
HHHHCCCCCEEEEEEEECCCCCCCCCCCCCCEECCCHHHHHHHHCCCCCCCCCCCCCCEE
AVKGM
EEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]