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The map label for this gene is rfbB [H]

Identifier: 66044181

GI number: 66044181

Start: 1053455

End: 1054555

Strand: Reverse

Name: rfbB [H]

Synonym: Psyr_0926

Alternate gene names: 66044181

Gene position: 1054555-1053455 (Counterclockwise)

Preceding gene: 66044182

Following gene: 66044180

Centisome position: 17.31

GC content: 58.13

Gene sequence:

>1101_bases
ATGATCCTCGTCACAGGCGGAGCCGGCTTCATCGGCTCGAATTTCGTTCTGCAGTGGTGCGCTCGTAATGGCGAGCCGGT
GTTGAACCTCGATGCGCTGACCTACGCTGGCAATCTGGCCAACCTGCAGTCGCTGGAAGGCAACGAGCAGCATCGTTTTG
TTCACGGCAATATTGGTGATGCTGCATTGCTGGACCGGCTGTTTGCCGAGCATCGTCCGCGGGCTGTGGTGCATTTCGCT
GCGGAATCGCATGTGGACCGTTCGATTACCGGTCCCGAGGCATTCGTCGAGACCAACGTGATGGGTACGTTCCGTTTGCT
GGAAGCGGCTCGTGCGTACTGGAACGGTCTGGAGGCGGATGACAAGGCTGCGTTCCGTTTCCTGCATGTGTCTACCGACG
AGGTCTATGGCACGCTGGGCGCGAACGATCCGGCTTTTACCGAGACCACGCCGTATCAGCCGAACAGCCCCTATTCGGCC
AGCAAGGCCGCCAGTGATCATTTGGTGCGTTCCTACCATCACACCTACGGCATGCCGGTGTTGACCACCAACTGTTCGAA
CAACTACGGGCCTTTCCACTTTCCGGAAAAGCTGATCCCGCTGATGATCGTCAACGCATTGGCCGGCAAGGCGCTGCCGG
TCTACGGTGATGGCCAGCAGATCCGCGACTGGTTGTACGTCGAAGATCATTGCTCAGGTATTCGCCGTGTTCTGGAAGCC
GGTGCGCTGGGCGAGACTTACAACATTGGCGGCTGGAACGAGAAGGCCAATATCGATATCGTGCAGACCCTGTGTACCCT
GCTCGATGAACTGGCTCCCGCCGCCGCGCGTCAGGTCATCAATCAGAAGACCGGTCAGCCGGTCAGCGCTTACGCCGAGC
TGATCACTTATGTAACCGATCGCCCGGGCCATGACCGCCGCTACGCGATCGATGCGCGCAAGATTGAACGCGAGCTGGGC
TGGAAGCCTGCAGAAACCTTTGAGACCGGTATTCGCAAGACGGTGGAATGGTATCTGACCAATCAGAAATGGGTCAGTGG
TGTCATGGACGGAAGTTACCGTGACTGGATCGTGCAACAGTATGAGGTCAGCGACGCATGA

Upstream 100 bases:

>100_bases
TTTTGGCAAACCGGGTATACCGTGCTTAGATCCAACGTATTTTATTTCTAGATTTAGGTACATAATATTTACGTCTACTT
TATAGACTGAGACCTACACT

Downstream 100 bases:

>100_bases
AGATTCTGCTGCTGGGCAAGAATGGGCAGGTTGGTTGGGAACTGCAGCGCGCTCTGGCGCCGCTGGGCGAGGTGATCGCG
CTGGATCGTCAGGGTGCCGA

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MILVTGGAGFIGSNFVLQWCARNGEPVLNLDALTYAGNLANLQSLEGNEQHRFVHGNIGDAALLDRLFAEHRPRAVVHFA
AESHVDRSITGPEAFVETNVMGTFRLLEAARAYWNGLEADDKAAFRFLHVSTDEVYGTLGANDPAFTETTPYQPNSPYSA
SKAASDHLVRSYHHTYGMPVLTTNCSNNYGPFHFPEKLIPLMIVNALAGKALPVYGDGQQIRDWLYVEDHCSGIRRVLEA
GALGETYNIGGWNEKANIDIVQTLCTLLDELAPAAARQVINQKTGQPVSAYAELITYVTDRPGHDRRYAIDARKIERELG
WKPAETFETGIRKTVEWYLTNQKWVSGVMDGSYRDWIVQQYEVSDA

Sequences:

>Translated_366_residues
MILVTGGAGFIGSNFVLQWCARNGEPVLNLDALTYAGNLANLQSLEGNEQHRFVHGNIGDAALLDRLFAEHRPRAVVHFA
AESHVDRSITGPEAFVETNVMGTFRLLEAARAYWNGLEADDKAAFRFLHVSTDEVYGTLGANDPAFTETTPYQPNSPYSA
SKAASDHLVRSYHHTYGMPVLTTNCSNNYGPFHFPEKLIPLMIVNALAGKALPVYGDGQQIRDWLYVEDHCSGIRRVLEA
GALGETYNIGGWNEKANIDIVQTLCTLLDELAPAAARQVINQKTGQPVSAYAELITYVTDRPGHDRRYAIDARKIERELG
WKPAETFETGIRKTVEWYLTNQKWVSGVMDGSYRDWIVQQYEVSDA
>Mature_366_residues
MILVTGGAGFIGSNFVLQWCARNGEPVLNLDALTYAGNLANLQSLEGNEQHRFVHGNIGDAALLDRLFAEHRPRAVVHFA
AESHVDRSITGPEAFVETNVMGTFRLLEAARAYWNGLEADDKAAFRFLHVSTDEVYGTLGANDPAFTETTPYQPNSPYSA
SKAASDHLVRSYHHTYGMPVLTTNCSNNYGPFHFPEKLIPLMIVNALAGKALPVYGDGQQIRDWLYVEDHCSGIRRVLEA
GALGETYNIGGWNEKANIDIVQTLCTLLDELAPAAARQVINQKTGQPVSAYAELITYVTDRPGHDRRYAIDARKIERELG
WKPAETFETGIRKTVEWYLTNQKWVSGVMDGSYRDWIVQQYEVSDA

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=341, Percent_Identity=36.0703812316716, Blast_Score=219, Evalue=4e-57,
Organism=Homo sapiens, GI42516563, Length=342, Percent_Identity=23.0994152046784, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI4504031, Length=351, Percent_Identity=23.9316239316239, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI56237023, Length=324, Percent_Identity=26.2345679012346, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI56118217, Length=324, Percent_Identity=26.2345679012346, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI189083684, Length=324, Percent_Identity=26.2345679012346, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI1788353, Length=369, Percent_Identity=60.9756097560976, Blast_Score=464, Evalue=1e-132,
Organism=Escherichia coli, GI48994969, Length=353, Percent_Identity=62.8895184135977, Blast_Score=456, Evalue=1e-129,
Organism=Escherichia coli, GI1786974, Length=363, Percent_Identity=27.8236914600551, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788366, Length=304, Percent_Identity=27.9605263157895, Blast_Score=86, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17568069, Length=347, Percent_Identity=34.0057636887608, Blast_Score=200, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI115532424, Length=352, Percent_Identity=32.1022727272727, Blast_Score=179, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17539532, Length=342, Percent_Identity=24.5614035087719, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17539422, Length=248, Percent_Identity=26.2096774193548, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17507723, Length=249, Percent_Identity=26.5060240963855, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI133901786, Length=248, Percent_Identity=26.2096774193548, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI133901788, Length=248, Percent_Identity=26.2096774193548, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17539424, Length=248, Percent_Identity=26.2096774193548, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI133901790, Length=248, Percent_Identity=26.6129032258064, Blast_Score=78, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6319493, Length=297, Percent_Identity=26.5993265993266, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21356223, Length=342, Percent_Identity=25.7309941520468, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI19923002, Length=377, Percent_Identity=26.525198938992, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24158427, Length=292, Percent_Identity=27.0547945205479, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 40698; Mature: 40698

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILVTGGAGFIGSNFVLQWCARNGEPVLNLDALTYAGNLANLQSLEGNEQHRFVHGNIGD
CEEEECCCCCHHHHHHHHHHHCCCCCEEEEHHHHHCCCCHHHHHCCCCCCCEEEECCCCH
AALLDRLFAEHRPRAVVHFAAESHVDRSITGPEAFVETNVMGTFRLLEAARAYWNGLEAD
HHHHHHHHHHCCCCEEEEEEHHHCCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHCCCCCC
DKAAFRFLHVSTDEVYGTLGANDPAFTETTPYQPNSPYSASKAASDHLVRSYHHTYGMPV
CHHEEEEEEEEHHHHEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCE
LTTNCSNNYGPFHFPEKLIPLMIVNALAGKALPVYGDGQQIRDWLYVEDHCSGIRRVLEA
EEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHH
GALGETYNIGGWNEKANIDIVQTLCTLLDELAPAAARQVINQKTGQPVSAYAELITYVTD
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHHHHHCC
RPGHDRRYAIDARKIERELGWKPAETFETGIRKTVEWYLTNQKWVSGVMDGSYRDWIVQQ
CCCCCCEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHEE
YEVSDA
EECCCC
>Mature Secondary Structure
MILVTGGAGFIGSNFVLQWCARNGEPVLNLDALTYAGNLANLQSLEGNEQHRFVHGNIGD
CEEEECCCCCHHHHHHHHHHHCCCCCEEEEHHHHHCCCCHHHHHCCCCCCCEEEECCCCH
AALLDRLFAEHRPRAVVHFAAESHVDRSITGPEAFVETNVMGTFRLLEAARAYWNGLEAD
HHHHHHHHHHCCCCEEEEEEHHHCCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHCCCCCC
DKAAFRFLHVSTDEVYGTLGANDPAFTETTPYQPNSPYSASKAASDHLVRSYHHTYGMPV
CHHEEEEEEEEHHHHEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCE
LTTNCSNNYGPFHFPEKLIPLMIVNALAGKALPVYGDGQQIRDWLYVEDHCSGIRRVLEA
EEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHH
GALGETYNIGGWNEKANIDIVQTLCTLLDELAPAAARQVINQKTGQPVSAYAELITYVTD
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHHHHHCC
RPGHDRRYAIDARKIERELGWKPAETFETGIRKTVEWYLTNQKWVSGVMDGSYRDWIVQQ
CCCCCCEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHEE
YEVSDA
EECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8253667 [H]