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The map label for this gene is gluQ [H]

Identifier: 66044089

GI number: 66044089

Start: 945989

End: 946876

Strand: Reverse

Name: gluQ [H]

Synonym: Psyr_0834

Alternate gene names: 66044089

Gene position: 946876-945989 (Counterclockwise)

Preceding gene: 66044090

Following gene: 66044088

Centisome position: 15.54

GC content: 62.84

Gene sequence:

>888_bases
ATGAAAAAACCGGCCTGTATCGGGCGCTTTGCGCCGACTCCCAGTGGTTACCTGCATTTTGGCTCGCTGGTCGCAGCCCT
CGCCTCCTATCTCGATGCGCGCGCGGCAGACGGTCAGTGGCTGTTGCGCATGGAGGATCTCGACCCGCCGCGTGAAGTAG
CCGGCGCGCAGGCGGGGATTCTCGAGACACTCGAGCGCTACGGTTTTGAATGGGACGGCCAGATGGTGCGGCAAAGCGAG
CGACATGACGCCTACGCCGAGATCCTCCAGCGCTGGTTCAATCATGGCCTGGCCTACGCCTGCACCTGCTCGCGCAAACA
GCTCGAAGGCTTTCAGGGTATTTATCCGGGTTTCTGCCGCAATGCCGGACATTCGCCAGACAACGCGGCGATCCGCATTC
GCGTACCGGAGCTGGAATATCGTTTCGACGACCGGGTTCAGGGCACCTTCAAAATGCACCTGGGCCGCGAAAGCGGTGAC
TTTGTGATCCGCAGGCGCGATGGGCTGTATGCCTATCAACTGGCCGTGGTCATTGACGACGCCTGGCAAGGCGTCACCGA
TATCGTCCGCGGCGCCGACCTGCTCGACTCGACCCCCCGCCAGCTGTACCTGCAGGAACTGCTGGGCCTGCCCCAACCGC
GCTACCTGCATGTACCGTTGATCACCCAGCCGGATGGCCACAAGCTGGGCAAGTCCTATCGCTCCCCACCGCTGCCGGCC
GATCAGGCGACGCCGCTGCTGCTGCGGGCCTTGCGCGCATTGGGGCAACCGCTCGATACGCACATGGCCGACGGCACTGC
ACAGGAAGTGCTTGCCTGGGGCATCCGTCACTGGAACGCTGACCTGATACCCCGACTGCGCAATATTGAAGAAGCCCGGA
TAGACTGA

Upstream 100 bases:

>100_bases
CGAACTGCATGCCAACGCGGAGCATTGGCACAAGACTCTGCATAACCACCCACTGCGTTCCACTTGCCCGCCCAAGCCCG
TCACCCGTAACATCCCGCCC

Downstream 100 bases:

>100_bases
TCCAGAGCGGTCGCCACAAACTGCGACGCAAGGATACTTGCAGGTTATCCGCCATCCGTTAACATGCCGCTCCTCATCTA
CCAGCGCAGGGAGACCAGAT

Product: glutamyl-Q tRNA(Asp) synthetase

Products: NA

Alternate protein names: Glu-Q-RSs [H]

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MKKPACIGRFAPTPSGYLHFGSLVAALASYLDARAADGQWLLRMEDLDPPREVAGAQAGILETLERYGFEWDGQMVRQSE
RHDAYAEILQRWFNHGLAYACTCSRKQLEGFQGIYPGFCRNAGHSPDNAAIRIRVPELEYRFDDRVQGTFKMHLGRESGD
FVIRRRDGLYAYQLAVVIDDAWQGVTDIVRGADLLDSTPRQLYLQELLGLPQPRYLHVPLITQPDGHKLGKSYRSPPLPA
DQATPLLLRALRALGQPLDTHMADGTAQEVLAWGIRHWNADLIPRLRNIEEARID

Sequences:

>Translated_295_residues
MKKPACIGRFAPTPSGYLHFGSLVAALASYLDARAADGQWLLRMEDLDPPREVAGAQAGILETLERYGFEWDGQMVRQSE
RHDAYAEILQRWFNHGLAYACTCSRKQLEGFQGIYPGFCRNAGHSPDNAAIRIRVPELEYRFDDRVQGTFKMHLGRESGD
FVIRRRDGLYAYQLAVVIDDAWQGVTDIVRGADLLDSTPRQLYLQELLGLPQPRYLHVPLITQPDGHKLGKSYRSPPLPA
DQATPLLLRALRALGQPLDTHMADGTAQEVLAWGIRHWNADLIPRLRNIEEARID
>Mature_295_residues
MKKPACIGRFAPTPSGYLHFGSLVAALASYLDARAADGQWLLRMEDLDPPREVAGAQAGILETLERYGFEWDGQMVRQSE
RHDAYAEILQRWFNHGLAYACTCSRKQLEGFQGIYPGFCRNAGHSPDNAAIRIRVPELEYRFDDRVQGTFKMHLGRESGD
FVIRRRDGLYAYQLAVVIDDAWQGVTDIVRGADLLDSTPRQLYLQELLGLPQPRYLHVPLITQPDGHKLGKSYRSPPLPA
DQATPLLLRALRALGQPLDTHMADGTAQEVLAWGIRHWNADLIPRLRNIEEARID

Specific function: Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5- dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in the wobble positi

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=248, Percent_Identity=34.6774193548387, Blast_Score=132, Evalue=3e-31,
Organism=Escherichia coli, GI226510925, Length=284, Percent_Identity=49.2957746478873, Blast_Score=282, Evalue=2e-77,
Organism=Escherichia coli, GI1788743, Length=238, Percent_Identity=35.7142857142857, Blast_Score=147, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI71993394, Length=248, Percent_Identity=29.8387096774194, Blast_Score=106, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6324540, Length=247, Percent_Identity=32.3886639676113, Blast_Score=140, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24665321, Length=248, Percent_Identity=32.6612903225806, Blast_Score=130, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022380
- InterPro:   IPR000924
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: NA

Molecular weight: Translated: 33285; Mature: 33285

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKPACIGRFAPTPSGYLHFGSLVAALASYLDARAADGQWLLRMEDLDPPREVAGAQAGI
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHCCHHHH
LETLERYGFEWDGQMVRQSERHDAYAEILQRWFNHGLAYACTCSRKQLEGFQGIYPGFCR
HHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHCCCEEEEECCHHHHHHCCCCCCHHHH
NAGHSPDNAAIRIRVPELEYRFDDRVQGTFKMHLGRESGDFVIRRRDGLYAYQLAVVIDD
CCCCCCCCCEEEEECCCCCCCCCCCCCEEEEEEECCCCCCEEEEECCCCEEEEEEEEECH
AWQGVTDIVRGADLLDSTPRQLYLQELLGLPQPRYLHVPLITQPDGHKLGKSYRSPPLPA
HHCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHCCCCCCCC
DQATPLLLRALRALGQPLDTHMADGTAQEVLAWGIRHWNADLIPRLRNIEEARID
CCCCHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHCCHHHCCC
>Mature Secondary Structure
MKKPACIGRFAPTPSGYLHFGSLVAALASYLDARAADGQWLLRMEDLDPPREVAGAQAGI
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHCCHHHH
LETLERYGFEWDGQMVRQSERHDAYAEILQRWFNHGLAYACTCSRKQLEGFQGIYPGFCR
HHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHCCCEEEEECCHHHHHHCCCCCCHHHH
NAGHSPDNAAIRIRVPELEYRFDDRVQGTFKMHLGRESGDFVIRRRDGLYAYQLAVVIDD
CCCCCCCCCEEEEECCCCCCCCCCCCCEEEEEEECCCCCCEEEEECCCCEEEEEEEEECH
AWQGVTDIVRGADLLDSTPRQLYLQELLGLPQPRYLHVPLITQPDGHKLGKSYRSPPLPA
HHCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHCCCCCCCC
DQATPLLLRALRALGQPLDTHMADGTAQEVLAWGIRHWNADLIPRLRNIEEARID
CCCCHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]