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The map label for this gene is purR [H]

Identifier: 66044032

GI number: 66044032

Start: 881642

End: 882655

Strand: Reverse

Name: purR [H]

Synonym: Psyr_0777

Alternate gene names: 66044032

Gene position: 882655-881642 (Counterclockwise)

Preceding gene: 66044036

Following gene: 66044031

Centisome position: 14.48

GC content: 63.51

Gene sequence:

>1014_bases
ATGACCAGACCCGTAACGCTCAAGGATGTTGCCAACCATGCAGGTCTTTCCAAGGCTGCGGTGTCGCGCTATCTGAACCG
AAGCATCAGCCTGCCGCCGGAAACCGCGCAACGTATCGACCTGGCGATCAAGGCGCTGGACTACCGGGGCAACAGTCTGG
CCCGGCGCCTGAGCAAAGGCGGCAGTGAGACCATCGGCCTGGTGCTGCCGGACATCGCCAACCCGTTTTTCGCCGAGCTG
GCAGACGCGGCCGAGGAAGAGGCGTCCGCACACGGTTATAACCTGGTGCTGTGTGTCACCCGCAACCTGCTTGAGCGCGA
AAGCACGTTTGTGCGTTGGCTGGATTCACGTAACGTCGATGGCTTGCTACTGGTCAGCAACCGCCCGGATGACGGCACGC
TGGCCAGCCAGCTGATGAGCTACCGCAATATCATTCTGCTCGACGAAGACGTTCCCGGCACGTCGCAGCCCAAAGTCTTC
GCCGACAACCATCAGGGCGGTCGCCTGGCGACTGACTATCTGATCCGCCATGGCCATCGACGCATCGCTCATGTGTCCGG
GCCGTCGGCCTTGATGAGTGCTCGCGAGCGCTATGCCGGCTACCGGGAAGCGCTCGCGCTGGCGAGCATCGCAGAGGTGC
CGGAATACCTGTGCTTTGGGGATTACAGCCGTGAGTTCGGGCGTATCGCGACCGCACAGCTACTCACCCTGCCCGACCCT
CCGGAGGCGATTTTCGCCGCCAGTGACTTCATCGCCCTGGGCGTGCTCGATGTCCTGCGTGAGCGCACTATCAGCGTGCC
CGGTGCCATGTCGCTGGTGGGTTTCGACGACGCCGTCTACGCCAGCCTGCTGACCCCGCCGCTGTGTACCATCCGCCAAT
CCTCCAGAGAGCTTGGCCGTCTCGGCGTGGCGCAACTGCTGCAGAGGATGACGGGCGGCGACTGTGCGCAGGACATCGTG
CGCGTACCCGTAGAGCTGGTCTGTCGGGGCACGGTAGCAACGCGCAGTGACTGA

Upstream 100 bases:

>100_bases
GTCACCCGCAGAGAAAATCGTCGGGCTGTTCGGGCACGCATGAGAAGATACCGGCACCTCACTTCAATACTTCGTTTTTC
AGGCCACAAAGCCGACCGCC

Downstream 100 bases:

>100_bases
TTGCGGGGCAACAAGTAACACCCGGCCTTAAATCAGTGCACTGCCGTTTGCCTCCTGAAAAGCCGATGATTGCGGAAAAC
TGTGGCGCATCAAGGCTGTA

Product: LacI transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MTRPVTLKDVANHAGLSKAAVSRYLNRSISLPPETAQRIDLAIKALDYRGNSLARRLSKGGSETIGLVLPDIANPFFAEL
ADAAEEEASAHGYNLVLCVTRNLLERESTFVRWLDSRNVDGLLLVSNRPDDGTLASQLMSYRNIILLDEDVPGTSQPKVF
ADNHQGGRLATDYLIRHGHRRIAHVSGPSALMSARERYAGYREALALASIAEVPEYLCFGDYSREFGRIATAQLLTLPDP
PEAIFAASDFIALGVLDVLRERTISVPGAMSLVGFDDAVYASLLTPPLCTIRQSSRELGRLGVAQLLQRMTGGDCAQDIV
RVPVELVCRGTVATRSD

Sequences:

>Translated_337_residues
MTRPVTLKDVANHAGLSKAAVSRYLNRSISLPPETAQRIDLAIKALDYRGNSLARRLSKGGSETIGLVLPDIANPFFAEL
ADAAEEEASAHGYNLVLCVTRNLLERESTFVRWLDSRNVDGLLLVSNRPDDGTLASQLMSYRNIILLDEDVPGTSQPKVF
ADNHQGGRLATDYLIRHGHRRIAHVSGPSALMSARERYAGYREALALASIAEVPEYLCFGDYSREFGRIATAQLLTLPDP
PEAIFAASDFIALGVLDVLRERTISVPGAMSLVGFDDAVYASLLTPPLCTIRQSSRELGRLGVAQLLQRMTGGDCAQDIV
RVPVELVCRGTVATRSD
>Mature_336_residues
TRPVTLKDVANHAGLSKAAVSRYLNRSISLPPETAQRIDLAIKALDYRGNSLARRLSKGGSETIGLVLPDIANPFFAELA
DAAEEEASAHGYNLVLCVTRNLLERESTFVRWLDSRNVDGLLLVSNRPDDGTLASQLMSYRNIILLDEDVPGTSQPKVFA
DNHQGGRLATDYLIRHGHRRIAHVSGPSALMSARERYAGYREALALASIAEVPEYLCFGDYSREFGRIATAQLLTLPDPP
EAIFAASDFIALGVLDVLRERTISVPGAMSLVGFDDAVYASLLTPPLCTIRQSSRELGRLGVAQLLQRMTGGDCAQDIVR
VPVELVCRGTVATRSD

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=332, Percent_Identity=34.0361445783133, Blast_Score=190, Evalue=1e-49,
Organism=Escherichia coli, GI1790194, Length=330, Percent_Identity=34.8484848484849, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1787948, Length=332, Percent_Identity=32.8313253012048, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1788474, Length=334, Percent_Identity=31.1377245508982, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI1789202, Length=287, Percent_Identity=31.7073170731707, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1787580, Length=321, Percent_Identity=28.9719626168224, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1786540, Length=332, Percent_Identity=32.2289156626506, Blast_Score=134, Evalue=6e-33,
Organism=Escherichia coli, GI1789068, Length=316, Percent_Identity=30.0632911392405, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1789456, Length=335, Percent_Identity=29.5522388059701, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1790715, Length=323, Percent_Identity=26.3157894736842, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI48994940, Length=314, Percent_Identity=27.7070063694268, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1787906, Length=343, Percent_Identity=26.8221574344023, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1790192, Length=278, Percent_Identity=29.8561151079137, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1786268, Length=303, Percent_Identity=22.1122112211221, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1790689, Length=334, Percent_Identity=23.3532934131737, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36661; Mature: 36530

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRPVTLKDVANHAGLSKAAVSRYLNRSISLPPETAQRIDLAIKALDYRGNSLARRLSKG
CCCCCCHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCC
GSETIGLVLPDIANPFFAELADAAEEEASAHGYNLVLCVTRNLLERESTFVRWLDSRNVD
CCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCC
GLLLVSNRPDDGTLASQLMSYRNIILLDEDVPGTSQPKVFADNHQGGRLATDYLIRHGHR
EEEEEECCCCCHHHHHHHHHHCCEEEEECCCCCCCCCCEEECCCCCCCHHHHHHHHCCCH
RIAHVSGPSALMSARERYAGYREALALASIAEVPEYLCFGDYSREFGRIATAQLLTLPDP
HEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEECCCC
PEAIFAASDFIALGVLDVLRERTISVPGAMSLVGFDDAVYASLLTPPLCTIRQSSRELGR
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHCCCHHHHHHHHHHHHH
LGVAQLLQRMTGGDCAQDIVRVPVELVCRGTVATRSD
HHHHHHHHHHCCCHHHHHHHHCCHHHHCCCCCCCCCC
>Mature Secondary Structure 
TRPVTLKDVANHAGLSKAAVSRYLNRSISLPPETAQRIDLAIKALDYRGNSLARRLSKG
CCCCCHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCC
GSETIGLVLPDIANPFFAELADAAEEEASAHGYNLVLCVTRNLLERESTFVRWLDSRNVD
CCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCC
GLLLVSNRPDDGTLASQLMSYRNIILLDEDVPGTSQPKVFADNHQGGRLATDYLIRHGHR
EEEEEECCCCCHHHHHHHHHHCCEEEEECCCCCCCCCCEEECCCCCCCHHHHHHHHCCCH
RIAHVSGPSALMSARERYAGYREALALASIAEVPEYLCFGDYSREFGRIATAQLLTLPDP
HEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEECCCC
PEAIFAASDFIALGVLDVLRERTISVPGAMSLVGFDDAVYASLLTPPLCTIRQSSRELGR
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHCCCHHHHHHHHHHHHH
LGVAQLLQRMTGGDCAQDIVRVPVELVCRGTVATRSD
HHHHHHHHHHCCCHHHHHHHHCCHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA