Click here to switch to the map view.

The map label for this gene is gshB [H]

Identifier: 66043754

GI number: 66043754

Start: 524260

End: 525219

Strand: Reverse

Name: gshB [H]

Synonym: Psyr_0487

Alternate gene names: 66043754

Gene position: 525219-524260 (Counterclockwise)

Preceding gene: 66043761

Following gene: 66043753

Centisome position: 8.62

GC content: 62.19

Gene sequence:

>960_bases
ATGAGCGTTCGCCTAGGGATTGTCATGGACCCCATTGAGCGCATTTCCTATAAAAAGGACAGCTCGCTGGCCATGCTGCT
TGCCGCCCAGGAACGCGGCTGGTCACTGTTCTATATGGAACAGAAAGACCTGTATCAGAACGCCGGCCAGGCCCGCGCAC
GCATGAAGCCGCTCAAGGTGTTTGCCGATCCGGCCAAGTGGTTCGAGTTCGAAGCCGAAGTCGACGCAGGTCTCGACGAT
CTGGACGTGATCCTGATGCGCAAGGATCCGCCGTTCGACATGGAGTTCGTCTACGCCACCTACCTGCTGGAGCAGGCCGA
GCGTGCCGGCGTCCTGGTGGTCAACAAGCCGCAGAGCCTGCGCGACTGCAACGAGAAGCTGTTCGCCACGCTGTTTCCGC
AGTGCACGCCGCCGACGCTGGTCAGCCGCCGCGCGGACATCATTCGCGAATTCGCCGAACAGCACGGCGACGTGATCCTC
AAGCCGCTGGACGGCATGGGCGGCGCATCGATCTTCCGCCATCGTGCGGGCGATCCGAACCTGTCAGTGATCCTCGAAAC
CCTGACCGCCCACGGCACGCAGCAAATCATGGCACAGGGTTACCTACCCGCCATCAAGGACGGCGACAAGCGCATTCTGA
TGGTCGATGGCGAACCGGTTCCGTATTGCCTGGCGCGCATCCCGGCAGCCGGCGAAACGCGTGGCAACCTAGCGGCAGGC
GGACGTGGCGAGGCCCGTCCATTGAGCGACAAGGACCGCTGGATCGCCGAACAGATCGGCCCGACCCTGCGTGAAAAAGG
CCTGCTGTTCGTCGGACTCGACGTGATCGGTGAGCACCTGACCGAAATCAACGTCACCAGCCCGACCTGCATCCGCGAAA
TCGACAACGCCTTCGGCACCAACATTGGTGGCCTGCTGATGGACGCGATCGAGAAGAAGCTGCAAGCGCGCAAGGGCTGA

Upstream 100 bases:

>100_bases
CATCGCGGCAGGCCTGCCCCTTGACCGGGAGCAATCGGAGCGCCACCCTGATGCCAATTTTACGGCCGTTGCGGCCCTTA
CCGATCAGAGGATATTGCCC

Downstream 100 bases:

>100_bases
TCAGCCGCTGACGCCGCTGGCCACGGGCAACACCGTGCGCCACGGCGTCGGATCGATTCCCTTCGTCCGATCCGCAGCAG
AGCCGCGCGGCACATTGCGT

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MSVRLGIVMDPIERISYKKDSSLAMLLAAQERGWSLFYMEQKDLYQNAGQARARMKPLKVFADPAKWFEFEAEVDAGLDD
LDVILMRKDPPFDMEFVYATYLLEQAERAGVLVVNKPQSLRDCNEKLFATLFPQCTPPTLVSRRADIIREFAEQHGDVIL
KPLDGMGGASIFRHRAGDPNLSVILETLTAHGTQQIMAQGYLPAIKDGDKRILMVDGEPVPYCLARIPAAGETRGNLAAG
GRGEARPLSDKDRWIAEQIGPTLREKGLLFVGLDVIGEHLTEINVTSPTCIREIDNAFGTNIGGLLMDAIEKKLQARKG

Sequences:

>Translated_319_residues
MSVRLGIVMDPIERISYKKDSSLAMLLAAQERGWSLFYMEQKDLYQNAGQARARMKPLKVFADPAKWFEFEAEVDAGLDD
LDVILMRKDPPFDMEFVYATYLLEQAERAGVLVVNKPQSLRDCNEKLFATLFPQCTPPTLVSRRADIIREFAEQHGDVIL
KPLDGMGGASIFRHRAGDPNLSVILETLTAHGTQQIMAQGYLPAIKDGDKRILMVDGEPVPYCLARIPAAGETRGNLAAG
GRGEARPLSDKDRWIAEQIGPTLREKGLLFVGLDVIGEHLTEINVTSPTCIREIDNAFGTNIGGLLMDAIEKKLQARKG
>Mature_318_residues
SVRLGIVMDPIERISYKKDSSLAMLLAAQERGWSLFYMEQKDLYQNAGQARARMKPLKVFADPAKWFEFEAEVDAGLDDL
DVILMRKDPPFDMEFVYATYLLEQAERAGVLVVNKPQSLRDCNEKLFATLFPQCTPPTLVSRRADIIREFAEQHGDVILK
PLDGMGGASIFRHRAGDPNLSVILETLTAHGTQQIMAQGYLPAIKDGDKRILMVDGEPVPYCLARIPAAGETRGNLAAGG
RGEARPLSDKDRWIAEQIGPTLREKGLLFVGLDVIGEHLTEINVTSPTCIREIDNAFGTNIGGLLMDAIEKKLQARKG

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=315, Percent_Identity=67.9365079365079, Blast_Score=438, Evalue=1e-124,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 35357; Mature: 35226

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVRLGIVMDPIERISYKKDSSLAMLLAAQERGWSLFYMEQKDLYQNAGQARARMKPLKV
CCEEECEEECHHHHHCCCCCCCEEEEEEECCCCCEEEEEEHHHHHHCHHHHHHHCCHHHE
FADPAKWFEFEAEVDAGLDDLDVILMRKDPPFDMEFVYATYLLEQAERAGVLVVNKPQSL
EECHHHHEEEECHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCEEEEECCHHH
RDCNEKLFATLFPQCTPPTLVSRRADIIREFAEQHGDVILKPLDGMGGASIFRHRAGDPN
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHCCCCCC
LSVILETLTAHGTQQIMAQGYLPAIKDGDKRILMVDGEPVPYCLARIPAAGETRGNLAAG
HHHHHHHHHHCCHHHHHHCCCCCCCCCCCCEEEEECCCCCCHHHHHCCCCCCCCCCCCCC
GRGEARPLSDKDRWIAEQIGPTLREKGLLFVGLDVIGEHLTEINVTSPTCIREIDNAFGT
CCCCCCCCCCHHHHHHHHHCHHHHHCCEEEEEHHHHHHHHHEECCCCCHHHHHHHHHHCC
NIGGLLMDAIEKKLQARKG
CHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SVRLGIVMDPIERISYKKDSSLAMLLAAQERGWSLFYMEQKDLYQNAGQARARMKPLKV
CEEECEEECHHHHHCCCCCCCEEEEEEECCCCCEEEEEEHHHHHHCHHHHHHHCCHHHE
FADPAKWFEFEAEVDAGLDDLDVILMRKDPPFDMEFVYATYLLEQAERAGVLVVNKPQSL
EECHHHHEEEECHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCEEEEECCHHH
RDCNEKLFATLFPQCTPPTLVSRRADIIREFAEQHGDVILKPLDGMGGASIFRHRAGDPN
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHCCCCCC
LSVILETLTAHGTQQIMAQGYLPAIKDGDKRILMVDGEPVPYCLARIPAAGETRGNLAAG
HHHHHHHHHHCCHHHHHHCCCCCCCCCCCCEEEEECCCCCCHHHHHCCCCCCCCCCCCCC
GRGEARPLSDKDRWIAEQIGPTLREKGLLFVGLDVIGEHLTEINVTSPTCIREIDNAFGT
CCCCCCCCCCHHHHHHHHHCHHHHHCCEEEEEHHHHHHHHHEECCCCCHHHHHHHHHHCC
NIGGLLMDAIEKKLQARKG
CHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]