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The map label for this gene is yafL [H]

Identifier: 66043746

GI number: 66043746

Start: 517592

End: 518269

Strand: Reverse

Name: yafL [H]

Synonym: Psyr_0479

Alternate gene names: 66043746

Gene position: 518269-517592 (Counterclockwise)

Preceding gene: 66043748

Following gene: 66043744

Centisome position: 8.5

GC content: 60.03

Gene sequence:

>678_bases
ATGCGTCCTTTTTTCAAGACATGGCTAACCATTTGCCTATTTATGCCTCTGGCCGCCCACGCCACCAATCGTGAGCAACA
ACTTCCTGCGAGCTTCTCGGGCTACACCGCCAAAAGCCACTCTTCACCGGCACTCGCGACTCGCAATGCACCCCAGGAAG
CCGCCACCACCCGAACTCAGTACGGCGCCACCGCTGCCGTCCAGAAGCCGCTTTCACGCAAGAACGCCAAGAAAGTCGCG
GCCTTGCAGGCTTCCGCCCCCGCCAAGCAAGGCAGCGCCGTTGTAAAGCGCGCCTTGCAGGCTGTGGGCACGCCTTATCG
CTGGGGCGGCACCACGCCTGGCAAAGGCCTGGACTGCAGCGGACTGGTCAAATACGCCTACACGGATGTCCGTGAGGTCG
ACCTGCCGCGCACCTCCAATGCCATGGCCCAAGGTCATGGTCAGACGGTGGATCGCAAGGACCTGAAGCCCGGTGATCTG
CTGTTCTTCAACATCAAGAGCCGCAACATCAACCACGTGGCCATTTACCTGGGCGACAACAAGTTCGTTCATGCGCCGCG
CCGTGGCAAGGCCGTAACTGTCGATACGCTGAACAAACCGTATTGGAACAGCCACTACAAGATTGCCAAGCGTGTGCTGC
CCAAGCAGGCAGGGCAGATGCGTGTGGTTCAGCGCTGA

Upstream 100 bases:

>100_bases
AAAACGCAATGATGCCACTGTGATGCGCTCGACAAATGGGGTCAGTTTTTTTGAAAAAGCTGTTATAAAGTTGCGCGCTG
ACCACCATGAGCCCTGCCGA

Downstream 100 bases:

>100_bases
TCCTGGCCTGAATCTGCGCTCTGCAGGCTGCATCTGAAAAAGGGGTCTTGAACGCGTTCAAGACCCCTTTTTCGTTCCTG
CAGCATCCCTGTGATCAGAA

Product: NLP/P60

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MRPFFKTWLTICLFMPLAAHATNREQQLPASFSGYTAKSHSSPALATRNAPQEAATTRTQYGATAAVQKPLSRKNAKKVA
ALQASAPAKQGSAVVKRALQAVGTPYRWGGTTPGKGLDCSGLVKYAYTDVREVDLPRTSNAMAQGHGQTVDRKDLKPGDL
LFFNIKSRNINHVAIYLGDNKFVHAPRRGKAVTVDTLNKPYWNSHYKIAKRVLPKQAGQMRVVQR

Sequences:

>Translated_225_residues
MRPFFKTWLTICLFMPLAAHATNREQQLPASFSGYTAKSHSSPALATRNAPQEAATTRTQYGATAAVQKPLSRKNAKKVA
ALQASAPAKQGSAVVKRALQAVGTPYRWGGTTPGKGLDCSGLVKYAYTDVREVDLPRTSNAMAQGHGQTVDRKDLKPGDL
LFFNIKSRNINHVAIYLGDNKFVHAPRRGKAVTVDTLNKPYWNSHYKIAKRVLPKQAGQMRVVQR
>Mature_225_residues
MRPFFKTWLTICLFMPLAAHATNREQQLPASFSGYTAKSHSSPALATRNAPQEAATTRTQYGATAAVQKPLSRKNAKKVA
ALQASAPAKQGSAVVKRALQAVGTPYRWGGTTPGKGLDCSGLVKYAYTDVREVDLPRTSNAMAQGHGQTVDRKDLKPGDL
LFFNIKSRNINHVAIYLGDNKFVHAPRRGKAVTVDTLNKPYWNSHYKIAKRVLPKQAGQMRVVQR

Specific function: Unknown

COG id: COG0791

COG function: function code M; Cell wall-associated hydrolases (invasion-associated proteins)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nlpC/p60 family [H]

Homologues:

Organism=Escherichia coli, GI1786421, Length=124, Percent_Identity=46.7741935483871, Blast_Score=129, Evalue=1e-31,
Organism=Escherichia coli, GI1787944, Length=121, Percent_Identity=42.1487603305785, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI1788501, Length=160, Percent_Identity=34.375, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1788001, Length=111, Percent_Identity=40.5405405405405, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000064 [H]

Pfam domain/function: PF00877 NLPC_P60 [H]

EC number: NA

Molecular weight: Translated: 24703; Mature: 24703

Theoretical pI: Translated: 11.14; Mature: 11.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPFFKTWLTICLFMPLAAHATNREQQLPASFSGYTAKSHSSPALATRNAPQEAATTRTQ
CCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCEECCCCCHHHHHHHHH
YGATAAVQKPLSRKNAKKVAALQASAPAKQGSAVVKRALQAVGTPYRWGGTTPGKGLDCS
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHH
GLVKYAYTDVREVDLPRTSNAMAQGHGQTVDRKDLKPGDLLFFNIKSRNINHVAIYLGDN
HHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEECCC
KFVHAPRRGKAVTVDTLNKPYWNSHYKIAKRVLPKQAGQMRVVQR
EEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEECCC
>Mature Secondary Structure
MRPFFKTWLTICLFMPLAAHATNREQQLPASFSGYTAKSHSSPALATRNAPQEAATTRTQ
CCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCEECCCCCHHHHHHHHH
YGATAAVQKPLSRKNAKKVAALQASAPAKQGSAVVKRALQAVGTPYRWGGTTPGKGLDCS
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHH
GLVKYAYTDVREVDLPRTSNAMAQGHGQTVDRKDLKPGDLLFFNIKSRNINHVAIYLGDN
HHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEECCC
KFVHAPRRGKAVTVDTLNKPYWNSHYKIAKRVLPKQAGQMRVVQR
EEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7596361; 9278503 [H]