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The map label for this gene is rsmE [H]

Identifier: 66043720

GI number: 66043720

Start: 488697

End: 489416

Strand: Reverse

Name: rsmE [H]

Synonym: Psyr_0453

Alternate gene names: 66043720

Gene position: 489416-488697 (Counterclockwise)

Preceding gene: 66043721

Following gene: 66043718

Centisome position: 8.03

GC content: 63.19

Gene sequence:

>720_bases
ATGAGACTGTCGCGCTTTTTTACTGAAACGCCGCTGAGTCTGGGCGAGCATGAACTGCCTGAGGCCCAGGCGCATTACAT
CAGCCGCGTGCTGCGCATGACCGAAGGTGACGCGCTGCAACTGTTCGACGGGTCGGGCCAGGAGTTTCGCGGCACGTTGC
TGGAAGTCGGCAAGAAGCGCGTGCATGTGCAGCTCACCGAAAGCTTCGCGGGGCAGGTGGAGTCAGGCCTGCGCATTCAT
CTCGGTCAAGGCCTGTCGCGCGGCGAGCGGATGGACTGGGCCATTCAAAAAGCCACCGAGCTGGGCGTCAGCGAAATTAC
GCCGATTGTCAGCGAACGCTGCGAGGTGCGCCTCAAGGACGAACGTGCCGAGAAGCGCCAGGCACACTGGCAGCAGATCG
CGATCAGCGCCTGTGAGCAATGTGGTCGCTCCGTGGTGCCGGTGATTCATGCACCGATGCCGCTGGCCGAGTGGATCAAG
CACACCGAAGCCGACCTGAAACTGGTCCTGCACCCGGTGGCCGAACCCCTGACCAGCCATGACAAGCCCGCCAGTCTGGC
CTTTCTGATTGGTCCGGAAGGCGGTCTGAACGATGCGGAAGTCACTCAGGCGCAAGACGCAGGCTTCCACGCCGCGCGCC
TCGGCCCACGCGTGCTGCGCACTGAAACCGCTCCCGTGGTCGCGCTCAGCGTGGCACAACAGCTGTGGGGCGATTTTTGA

Upstream 100 bases:

>100_bases
TTCGCGGACAAGTCCGCTCCTACAGGGGCCTGGCTGCACATGCGGGAGCGAGCCGGGTGACGACACCCTCACAGAATGAA
CACCGGAACCGAGACACACC

Downstream 100 bases:

>100_bases
TCATTTGATGCGTCCTCAAACGCGCTGCACGCTCAGAGTCAGACACCGGCTCGCTCCCACAAGGATTTGCATTTACACAG
CCATTGAGGCCTGAACCTGG

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MRLSRFFTETPLSLGEHELPEAQAHYISRVLRMTEGDALQLFDGSGQEFRGTLLEVGKKRVHVQLTESFAGQVESGLRIH
LGQGLSRGERMDWAIQKATELGVSEITPIVSERCEVRLKDERAEKRQAHWQQIAISACEQCGRSVVPVIHAPMPLAEWIK
HTEADLKLVLHPVAEPLTSHDKPASLAFLIGPEGGLNDAEVTQAQDAGFHAARLGPRVLRTETAPVVALSVAQQLWGDF

Sequences:

>Translated_239_residues
MRLSRFFTETPLSLGEHELPEAQAHYISRVLRMTEGDALQLFDGSGQEFRGTLLEVGKKRVHVQLTESFAGQVESGLRIH
LGQGLSRGERMDWAIQKATELGVSEITPIVSERCEVRLKDERAEKRQAHWQQIAISACEQCGRSVVPVIHAPMPLAEWIK
HTEADLKLVLHPVAEPLTSHDKPASLAFLIGPEGGLNDAEVTQAQDAGFHAARLGPRVLRTETAPVVALSVAQQLWGDF
>Mature_239_residues
MRLSRFFTETPLSLGEHELPEAQAHYISRVLRMTEGDALQLFDGSGQEFRGTLLEVGKKRVHVQLTESFAGQVESGLRIH
LGQGLSRGERMDWAIQKATELGVSEITPIVSERCEVRLKDERAEKRQAHWQQIAISACEQCGRSVVPVIHAPMPLAEWIK
HTEADLKLVLHPVAEPLTSHDKPASLAFLIGPEGGLNDAEVTQAQDAGFHAARLGPRVLRTETAPVVALSVAQQLWGDF

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=242, Percent_Identity=47.1074380165289, Blast_Score=199, Evalue=1e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 26390; Mature: 26390

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSRFFTETPLSLGEHELPEAQAHYISRVLRMTEGDALQLFDGSGQEFRGTLLEVGKKR
CCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCHH
VHVQLTESFAGQVESGLRIHLGQGLSRGERMDWAIQKATELGVSEITPIVSERCEVRLKD
EEEEEHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCEEEHH
ERAEKRQAHWQQIAISACEQCGRSVVPVIHAPMPLAEWIKHTEADLKLVLHPVAEPLTSH
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHCHHHHHHHHHHHHHHHHCC
DKPASLAFLIGPEGGLNDAEVTQAQDAGFHAARLGPRVLRTETAPVVALSVAQQLWGDF
CCCCEEEEEECCCCCCCCHHHHHHHHCCCHHHHCCCHHHHCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRLSRFFTETPLSLGEHELPEAQAHYISRVLRMTEGDALQLFDGSGQEFRGTLLEVGKKR
CCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCHH
VHVQLTESFAGQVESGLRIHLGQGLSRGERMDWAIQKATELGVSEITPIVSERCEVRLKD
EEEEEHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCEEEHH
ERAEKRQAHWQQIAISACEQCGRSVVPVIHAPMPLAEWIKHTEADLKLVLHPVAEPLTSH
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHCHHHHHHHHHHHHHHHHCC
DKPASLAFLIGPEGGLNDAEVTQAQDAGFHAARLGPRVLRTETAPVVALSVAQQLWGDF
CCCCEEEEEECCCCCCCCHHHHHHHHCCCHHHHCCCHHHHCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8332061 [H]