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The map label for this gene is gltD [H]

Identifier: 66043679

GI number: 66043679

Start: 446857

End: 448275

Strand: Direct

Name: gltD [H]

Synonym: Psyr_0412

Alternate gene names: 66043679

Gene position: 446857-448275 (Clockwise)

Preceding gene: 66043678

Following gene: 66043680

Centisome position: 7.33

GC content: 61.31

Gene sequence:

>1419_bases
ATGGCTGAACGTCTCAGTAACGACTTCCAGTTCATCGACGTCGGACGTAAAGACCCGAAGAAGAAACTGCTGCGTCAACG
CAAGAAAGAGTTCGTGGAAATCTACGAGCCTTTCAAGCCCCAGCAGTCCGCCGATCAGGCGCACCGCTGCCTGGGTTGCG
GCAACCCGTACTGCGAATGGAAGTGCCCGGTGCACAACTTCATTCCCAACTGGCTCAAGCTGGTCGCCGAGGGCAATATC
CTCGCGGCAGCGGAATTGTCGCACCAGACCAACACCCTGCCGGAAGTCTGCGGCCGCGTGTGCCCGCAGGATCGCCTGTG
TGAAGGTGCCTGCACACTGAACGACGGTTTCGGTGCGGTGACCATCGGGTCGGTCGAGAAGTACATCACCGATACCGCCT
TTGCCATGGGCTGGCGTCCGGACATGTCCAAGGTCGTGCCGACCGGCAAGCGCGTCGCGATCATCGGCGCAGGGCCTGCG
GGCCTGGGCTGTGCCGACGTGCTGGTGCGTGGCGGCGTAACCCCGGTGGTGTTCGACAAGAATCCGGAAATCGGCGGTCT
GCTGACCTTCGGCATTCCGGAATTCAAGCTGGAGAAATCCGTGCTGAGCCATCGCCGTGAAGTGTTCACCGGCATGGGCA
TCGAATTCCGTCTCAACACCGAAGTCGGCAAGGATGTCACCATCGATCAACTGCTCGCAGAATACGATGCGGTGTTCATG
GGCATGGGCACCTACACCTACATGAAAGGCGGCTTCCCCGGTGAGGATCTGCCAGGCGTGCACGATGCGCTCGACTTCCT
GATCGCCAACGTCAACCGCAACCTGGGCTTTGAAAAGGCCCCGGAAGACTTCGTCGACATGAAGGGCAAGCGGGTCGTGG
TTCTGGGCGGTGGCGACACGGCGATGGACTGCAACCGCACCTCTATCCGCCAGGGCGCCAAGTCAGTGACCTGCGCCTAC
CGTCGCGACGAAGAAAACATGCCTGGCTCGCGCAAGGAAGTGAAAAACGCCAAGGAAGAAGGCGTGAAGTTCCTCTACAA
CCGTCAGCCGATCGCCATCATCGGCGAGGGCAAGGTTGAAGGCGTGAAAGTGGTCGAGACGCGCCTGGGCGAACCGGATG
CCCGTGGTCGTCGCAGTCCCGAGCCTATTCCGGGCTCCGAAGAGATCATCCCGGCAGAAGCCGTGGTCATCGCCTTCGGC
TTCCGCCCAAGCCCGGCACCCTGGTTCGAGCAGTTCACCATCAGCACCGACAGCCAGGGCCGTGTCGTGGCCCCGGAACA
GGCGCAGTACAAGCACCAGACCAGCAACCCGAAGATCTTCGCCGGTGGCGACATGGTGCGCGGCTCCGACCTGGTGGTGA
CCGCTATCTTCGAAGGCCGTAATGCGGCTGAAGGGATTCTGGATTACCTGGGCGTCTGA

Upstream 100 bases:

>100_bases
AGCTTCAAGCCGCAAGTTTGCCACCCTGTGAATTGAACTGAATTGATTTCTTGCAGCTTGAAGCTTGTCGCTTGAAGCTG
CCGCAAAGAGGCTCCAGAGA

Downstream 100 bases:

>100_bases
GCCTTGTGTTGCCGGGGTGTGTCGTTGCTGACGCGCACCCCGATAACAAGCGTCTACCCTTCATAGGCACTGTGCCAATG
TCTCTGACATCCCGCCTGGC

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MAERLSNDFQFIDVGRKDPKKKLLRQRKKEFVEIYEPFKPQQSADQAHRCLGCGNPYCEWKCPVHNFIPNWLKLVAEGNI
LAAAELSHQTNTLPEVCGRVCPQDRLCEGACTLNDGFGAVTIGSVEKYITDTAFAMGWRPDMSKVVPTGKRVAIIGAGPA
GLGCADVLVRGGVTPVVFDKNPEIGGLLTFGIPEFKLEKSVLSHRREVFTGMGIEFRLNTEVGKDVTIDQLLAEYDAVFM
GMGTYTYMKGGFPGEDLPGVHDALDFLIANVNRNLGFEKAPEDFVDMKGKRVVVLGGGDTAMDCNRTSIRQGAKSVTCAY
RRDEENMPGSRKEVKNAKEEGVKFLYNRQPIAIIGEGKVEGVKVVETRLGEPDARGRRSPEPIPGSEEIIPAEAVVIAFG
FRPSPAPWFEQFTISTDSQGRVVAPEQAQYKHQTSNPKIFAGGDMVRGSDLVVTAIFEGRNAAEGILDYLGV

Sequences:

>Translated_472_residues
MAERLSNDFQFIDVGRKDPKKKLLRQRKKEFVEIYEPFKPQQSADQAHRCLGCGNPYCEWKCPVHNFIPNWLKLVAEGNI
LAAAELSHQTNTLPEVCGRVCPQDRLCEGACTLNDGFGAVTIGSVEKYITDTAFAMGWRPDMSKVVPTGKRVAIIGAGPA
GLGCADVLVRGGVTPVVFDKNPEIGGLLTFGIPEFKLEKSVLSHRREVFTGMGIEFRLNTEVGKDVTIDQLLAEYDAVFM
GMGTYTYMKGGFPGEDLPGVHDALDFLIANVNRNLGFEKAPEDFVDMKGKRVVVLGGGDTAMDCNRTSIRQGAKSVTCAY
RRDEENMPGSRKEVKNAKEEGVKFLYNRQPIAIIGEGKVEGVKVVETRLGEPDARGRRSPEPIPGSEEIIPAEAVVIAFG
FRPSPAPWFEQFTISTDSQGRVVAPEQAQYKHQTSNPKIFAGGDMVRGSDLVVTAIFEGRNAAEGILDYLGV
>Mature_471_residues
AERLSNDFQFIDVGRKDPKKKLLRQRKKEFVEIYEPFKPQQSADQAHRCLGCGNPYCEWKCPVHNFIPNWLKLVAEGNIL
AAAELSHQTNTLPEVCGRVCPQDRLCEGACTLNDGFGAVTIGSVEKYITDTAFAMGWRPDMSKVVPTGKRVAIIGAGPAG
LGCADVLVRGGVTPVVFDKNPEIGGLLTFGIPEFKLEKSVLSHRREVFTGMGIEFRLNTEVGKDVTIDQLLAEYDAVFMG
MGTYTYMKGGFPGEDLPGVHDALDFLIANVNRNLGFEKAPEDFVDMKGKRVVVLGGGDTAMDCNRTSIRQGAKSVTCAYR
RDEENMPGSRKEVKNAKEEGVKFLYNRQPIAIIGEGKVEGVKVVETRLGEPDARGRRSPEPIPGSEEIIPAEAVVIAFGF
RPSPAPWFEQFTISTDSQGRVVAPEQAQYKHQTSNPKIFAGGDMVRGSDLVVTAIFEGRNAAEGILDYLGV

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=447, Percent_Identity=28.6353467561521, Blast_Score=152, Evalue=9e-37,
Organism=Homo sapiens, GI111118983, Length=158, Percent_Identity=31.6455696202532, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI111118981, Length=158, Percent_Identity=31.6455696202532, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI1789606, Length=470, Percent_Identity=70, Blast_Score=681, Evalue=0.0,
Organism=Escherichia coli, GI1788811, Length=462, Percent_Identity=62.1212121212121, Blast_Score=609, Evalue=1e-175,
Organism=Escherichia coli, GI87082180, Length=456, Percent_Identity=56.359649122807, Blast_Score=524, Evalue=1e-150,
Organism=Escherichia coli, GI1788468, Length=444, Percent_Identity=30.8558558558559, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1789243, Length=419, Percent_Identity=29.1169451073986, Blast_Score=135, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17570289, Length=493, Percent_Identity=35.2941176470588, Blast_Score=248, Evalue=4e-66,
Organism=Caenorhabditis elegans, GI71984108, Length=473, Percent_Identity=29.3868921775898, Blast_Score=148, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI17543860, Length=207, Percent_Identity=28.9855072463768, Blast_Score=75, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6320030, Length=493, Percent_Identity=31.8458417849899, Blast_Score=226, Evalue=9e-60,
Organism=Drosophila melanogaster, GI28574881, Length=482, Percent_Identity=37.9668049792531, Blast_Score=280, Evalue=1e-75,
Organism=Drosophila melanogaster, GI24665539, Length=482, Percent_Identity=37.9668049792531, Blast_Score=280, Evalue=1e-75,
Organism=Drosophila melanogaster, GI24665547, Length=482, Percent_Identity=37.9668049792531, Blast_Score=280, Evalue=2e-75,
Organism=Drosophila melanogaster, GI24665543, Length=482, Percent_Identity=37.9668049792531, Blast_Score=280, Evalue=2e-75,
Organism=Drosophila melanogaster, GI24640763, Length=444, Percent_Identity=27.2522522522523, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI18858217, Length=444, Percent_Identity=27.2522522522523, Blast_Score=146, Evalue=3e-35,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR009051
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 51697; Mature: 51565

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAERLSNDFQFIDVGRKDPKKKLLRQRKKEFVEIYEPFKPQQSADQAHRCLGCGNPYCEW
CCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCEE
KCPVHNFIPNWLKLVAEGNILAAAELSHQTNTLPEVCGRVCPQDRLCEGACTLNDGFGAV
ECCHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHCCHHHHCCCCEEECCCCCEE
TIGSVEKYITDTAFAMGWRPDMSKVVPTGKRVAIIGAGPAGLGCADVLVRGGVTPVVFDK
EECCHHHHHHHHHHHCCCCCCHHHHCCCCCEEEEEECCCCCCCHHHHHHHCCCCEEEECC
NPEIGGLLTFGIPEFKLEKSVLSHRREVFTGMGIEFRLNTEVGKDVTIDQLLAEYDAVFM
CCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHC
GMGTYTYMKGGFPGEDLPGVHDALDFLIANVNRNLGFEKAPEDFVDMKGKRVVVLGGGDT
CCCHHEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCEEEEECCCCC
AMDCNRTSIRQGAKSVTCAYRRDEENMPGSRKEVKNAKEEGVKFLYNRQPIAIIGEGKVE
CCCCCHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCHHEEECCCCEEEEECCCCC
GVKVVETRLGEPDARGRRSPEPIPGSEEIIPAEAVVIAFGFRPSPAPWFEQFTISTDSQG
CEEEEEHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHEEEECCCCCC
RVVAPEQAQYKHQTSNPKIFAGGDMVRGSDLVVTAIFEGRNAAEGILDYLGV
CEECCHHHHHHHCCCCCEEEECCCEECCCCEEEEEEECCCCHHHHHHHHHCC
>Mature Secondary Structure 
AERLSNDFQFIDVGRKDPKKKLLRQRKKEFVEIYEPFKPQQSADQAHRCLGCGNPYCEW
CCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCEE
KCPVHNFIPNWLKLVAEGNILAAAELSHQTNTLPEVCGRVCPQDRLCEGACTLNDGFGAV
ECCHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHCCHHHHCCCCEEECCCCCEE
TIGSVEKYITDTAFAMGWRPDMSKVVPTGKRVAIIGAGPAGLGCADVLVRGGVTPVVFDK
EECCHHHHHHHHHHHCCCCCCHHHHCCCCCEEEEEECCCCCCCHHHHHHHCCCCEEEECC
NPEIGGLLTFGIPEFKLEKSVLSHRREVFTGMGIEFRLNTEVGKDVTIDQLLAEYDAVFM
CCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHC
GMGTYTYMKGGFPGEDLPGVHDALDFLIANVNRNLGFEKAPEDFVDMKGKRVVVLGGGDT
CCCHHEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCEEEEECCCCC
AMDCNRTSIRQGAKSVTCAYRRDEENMPGSRKEVKNAKEEGVKFLYNRQPIAIIGEGKVE
CCCCCHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCHHEEECCCCEEEEECCCCC
GVKVVETRLGEPDARGRRSPEPIPGSEEIIPAEAVVIAFGFRPSPAPWFEQFTISTDSQG
CEEEEEHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHEEEECCCCCC
RVVAPEQAQYKHQTSNPKIFAGGDMVRGSDLVVTAIFEGRNAAEGILDYLGV
CEECCHHHHHHHCCCCCEEEECCCEECCCCEEEEEEECCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3326786; 9278503; 9298646; 9600841; 2643092 [H]